• Title/Summary/Keyword: Serum

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Immunoradiometric Assay using Monoclonal Antibody Against Human Serum Transferrin Receptor for Diagnosis of Iron Deficiency (사람 혈청 트란스페린수용체의 단클론 항체를 이용한 방사면역측정과 철영양상태의 진단)

  • 김승렬
    • Journal of Nutrition and Health
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    • v.29 no.9
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    • pp.971-980
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    • 1996
  • The soluble transferrin receptor(TfR) in human serum has been shown recently to be a truncated form of intact membrane bound receptor containing most of the extracellular domain. We purfied the transferin-free TfR from human serum by immounoaffinity chromatography which produced the single protein identity in high resolution gel chormatography. The monoclonal antibodies(MAb) against purifed serum TfR were produced by fusion of spleen cells o fimmunized Balb/c mice and SP2 cells. Ten hybrids producing MAb specific for serum TfR were identifed and determine their iostypes. A immunoraddiometric assay (IRMA) for serum TfR was established using two monoclonal IgG1 antibodies as the coating and indicator antibodies on the bosis of their suitability in sandwich IRMA of serum TfR. The mean serum TfR levels in the 15 normal male, 15 normal female, and 19 iron-deficient subjects were 5.4$\pm$0.98, 4.6$\pm$0/76, and 18.0$\pm$12.8mg/1, respectively, and the difference in mean values between normal and iron deficient subjects was significant(p=0.0005). There existed the inverse logarithmic relationship(r=-0.9336, p<0.0001) between the serum TfR and ferritin levels.

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Serum Vitamin A Response to SHort-term Carotenoids Foods Supplementation in Female University Students (여대생의 Carotenoids 식품 보충 섭취에 따른 혈청 비타민 A 함량 변화)

  • 나유경;김영남
    • Journal of Nutrition and Health
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    • v.30 no.8
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    • pp.952-959
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    • 1997
  • Korean intakes of Vitamin A have not reached RDA for a long time according to the National Nutrition Survey. The pupose of this study was to examine the serum response to short-term carotenoids-rich vegetable juices supplementation. A total of 31 female students were recruited for this study. Their average serum retinol content before supplementation began was 14.74$\mu\textrm{g}$/100ml, and 87% of them had lower than adequate levels. Serum $\beta$-carotene, $\alpha$-carotene, lycopene and lutein contents were 0.77, -, 0.74 and 8.29$\mu\textrm{g}$/100$m\ell$ respectively. The serum responses to 3 kinds of juice supplementation for 3 weeks are summarized as follows. A small but steady increase in serum retinol concentration was detected with the supplementation (p<.05), but the kinds of juice made no significant difference. Serum $\beta$-carotene contents also increased with the supplementation(p<.01), but the kinds of juice again made no significant difference. Serum lycopene increased with the lycopene-containing vegetable juice and tomato juice supplementation, but the increase wasn't significant. There was no change in serum lycopene levels with the lycopene free carrot juice supplementation. Finally, there were no signifnicant changes in serum lutein and $\alpha$-carotene levels with the 3 kinds of juice supplementation.

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Effect of Mulberry Fruit Tea on the Serum Lipid Profiles and Cardiovascular Disease Markers of Middle-Aged People Living in Choongnam (오디 분말차 급여가 일부 충남지역에 거주하는 중년 남.녀의 혈청 지질 및 심혈관계 인자에 미친 영향)

  • Kim Ae-Jung;Yuh Chung-Suk;Bang In-Soo
    • Journal of the East Asian Society of Dietary Life
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    • v.16 no.4
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    • pp.408-413
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    • 2006
  • This study was conducted to examine the effects of mulberry fruit tea (MFT) on the levels of serum lipid profiles and serum cardiovascular disease markers in sixty middle-aged people(30 males and 30 females) who consumed MFT for 4 weeks. Anthropometric measurements, serum lipid profile levels, and serum cardiovascular disease markers were analyzed before and after consumption of MFT. After consumption of MFT, no significant differences were observed in anthropometric measurements, levels of serum aspartate transaminase (AST) and alanine transaminase (ALT) in males and females. Among serum lipid profiles, triglyceride and LDL-cholesterol were decreased significantly, whereas HDL-cholesterol was significantly increased.

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An Effect of Carbon Tetrachloride Treatment on the Serum Levels of Acid Phosphatase Activity in Rats (흰쥐에 사염화탄소 투여가 혈청 Acid Phosphatase활성에 미치는 영향)

  • 윤종국;신중규;차상은
    • Journal of Environmental Health Sciences
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    • v.17 no.2
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    • pp.121-126
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    • 1991
  • To clarify a cause of increased serum level of acid phosphatase in CCl$_{4}$-treated rats, the acid phosphatase activity of liver was compared with that serum. Concomitantly, the serum and liver acid phosphatase activity of CCl$_{4}$-treated rats were compared with that of CCl$_{4}$-treated rats pretreated with prednisolone or actinomycin D. In CCl$_{4}$-treated rats, the activity of serum acid phosphatase was significiantly increased whereas that of liver acid phosphatase was rather slightly decreased. the pretreatment of prednisolone led to the decreased activity of serum and liver acid phosphatase in CCl$_{4}$-treated rats. But the pretreatment of actinomycin D rather increased the activity of liver and serum enzyme. In conclusion, it is likely the increased activity of serum acid phosphatase is based on the excess leaking of acid phosphatase into blood by the increased membrane permeability of both liver cell and lysosome in it.

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Immunological ObservatioIn on Gandidiasis (Candida증(症) 발생(發生)의 면역학적(免疫學的) 기전(機轉)에 관(關)한 연구(硏究))

  • Kim, Hong-Sik
    • The Korean Journal of Mycology
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    • v.6 no.2
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    • pp.15-18
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    • 1978
  • The study was performed to investigate the inhibitory action on the growth rate of Candida albicans under the various immunological conditions, Candida albicans was grown in broth media containing 0.1ml. (in total broth volume 4.0ml.) of normal human serum, diabetic patient serum, albumin solution, artifical bulla content and nutrient solution as control subject. The inhibitory action of Candida albicans was indicated by mesurement of transparency rate with use of the spectrophotometer. The results are as follows: 1. Normal human serum shows inhibitory effect on the growth of the candida distinctly. 2. Albumin solution reveals almost similar to that of the effect of normal human serum. 3. Artificial bulla content which obtained by irradiation of ultraviolot ray after application of 1% 8-Methoxy-psolaren cream and the diabetic patient serum shows reduction of inhibitory effect as compared with that of the normal human serum. 4. It is estimated that the titer of negative effect of diabetic patient serum is not related with the variation of immunoglobulin titer in patient serum.

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Effect of Zinc Supplementation on Serum Cholesterol Concentration of Young Women (아연 보충이 젊은 여성의 혈청 콜레스테롤 농도에 미치는 영향)

  • 송미영
    • Journal of Nutrition and Health
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    • v.23 no.4
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    • pp.237-247
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    • 1990
  • This study was to investigate the effect of zinc supplementation on serum cholesterol concentration of young women. Thirty healthy students were divided into Zn and placebo groups, and were orally given with zinc(50mg/day, 220mg as ZnSO4·7H2O) or placebo for 2 month (June 9-August 7, 1988). Changes of plasma zinc, serum total cholesterol, HDL-cholesterol (HDL-C), LDL-cholesterol(LDL-C) and total lipid were analyzed from the initiation to 1 month after the end of zinc supplementation at monthly interval. Plasma zinc, serum LDL-C content and LDL-C/HDL-C were significantly increased by zinc supplementation. Serum total cholesterol content tended to be increased by zinc supplementation but was not significantly different between the two groups. Serum HDL-C content was significantly decreased by zinc supplementation. Serum total lipid content was not different between the two groups during experimental period. Thus, in this study considering the effect of zinc supplementation on serum cholesterol concentration, we conclude that the effect of zinc supplementation on coronary heart disease may be negative.

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Effect of Dietary Protein and Magnesium Levels on Serum Lipid Contents and Enzyme Activities in Rats (단백질과 마그네슘 공급수준이 흰쥐의 혈청중 지질함량 및 효소활성에 미치는 영향)

  • 정복미
    • Journal of Nutrition and Health
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    • v.26 no.8
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    • pp.925-932
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    • 1993
  • To study the effects of dietary protein and magnesium level on serum lipid contents and enzyme activities, male Sprague-Dawley rats were divided into six groups. Six groups of animals were fed six experimental diets different with protein(8% and 20%) and magnesium levels (Mg-free, 400mg/kg diet and 800mg/kg diet). The serum lipid contents and enzyme activities of rats fed different dietary protein and magnesium levels were examined. Serum triglyceride contents was significantly decreased as the dietary magnesium level was increased. Serum phospholipid contents was significantly higher in magnesium deficient group than those in the other groups. Serum alkaline phosphatase activity was significantly decreased in magnesium dificient group compared with other groups, but serum glutamic oxaloacetic transaminase and glutamic pyruvic transaminase activities was significantly increased in magnesium dificient group than other groups. In conclusion, serum triglyceride contents in low protein level was remarkably decreased as the dietary magnesium level was increased.

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Effects of Dokwhalgisaentang on the Blood in Rats (독활기생탕(獨活寄生湯)이 백서(白鼠)의 혈액(血液)에 미치는 영향(影響))

  • Kim, Hui-Cheol
    • The Journal of Internal Korean Medicine
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    • v.16 no.2
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    • pp.51-60
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    • 1995
  • In order to study the effect of Dokwhalgisaentang on the blood in normal rats with Dokwhalgisaentang extract solution was adminstrated in rats. The rats carried on every day for 5days, l0days and 15days respecitively on the corresponding blood in rats, and thereafter the contents of serum BUN, creatinine, creatine, total protein, albumin, total bilirubin, cholesterol were measured in rats. The following results have been obtained : 1. Dokwhalgisaentang extract decreased the serum BUN significantly in rats. 2. Dokwhalgisaentang extract decreased the serum creatine significantly in rats. 3. Dokwhalgisaentang extract decreased the serum creatinine significantly in rats. 4. Dokwhalgisaentang extract decreased the serum total protein significantly in rats. 5. Dokwhalgisaentang extract decreased the serum albumin significantly in rats. 6. Dokwhalgisaentang extract increased the serum total bilirubin significantly in rats. 7. Dokwhalgisaentang extract decreased the serum cholresterol significantly in rats.

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STUDIES ON THE EFFECT OF THE BUPLEURI RADIX ON THE SERUM TRANSAMINASE ACTIVITIES AND THE CONTENTS OF TOTAL CHOLESTEROL IN SERUM FROM THE ALLOXAN-DIABETIC RABBITS (시호(柴胡)가 Alloxan 투여가토혈청중(投與家兎血淸中) Cholesterol 함량(含量) 및 Transaminase 활성도(活性度)에 미치는 영향(影響))

  • Rhee, Kyung-Sup
    • The Journal of Internal Korean Medicine
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    • v.1 no.1
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    • pp.35-43
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    • 1976
  • The effects of root of the Bupleurum falcatum L. on the serum transaminase (SGP-T, SGO-T) activities and the content of total cholesterol in serum from normal and alloxan-diabetic rabbits were observed. The preparations were extracted by methanol, and extracted samples were orally administrated to the normal and alloxandiabetic rabbits. The transaminase activities and the content of total cholesterol in serum of normal rabbits were significantly increased by administration of the alloxan. The increased serum transaminase activities of the alloxandiabetic rabbits were significantly decreased after 96 hours by administration of the extracted samples. The increased content of total cholesterol in serum of the alloxan-diabetic rabbits were gradually decreased by administration of the extracted samples and were recovered to the control levels after 12 days. According to the above results, it would be concluded that the extract of Bupleuri Radix influences for the recovery of the increased contents of total cholesterol and serum transaminase activities by alloxan.

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Effect of Bile Deprivation on Serum Lipids and Gastrin in the Rat (흰쥐의 담즙 상실이 혈청 지질과 Gastrin 대사에 미치는 영향)

  • 송경희
    • Journal of Nutrition and Health
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    • v.20 no.1
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    • pp.46-53
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    • 1987
  • The effect of bile deprivation on serum lipid and gastrin contents was investigat\ulcornered after choledocho-urinary cystostomy in Sprague-Dawley rats. Bile deprivated rats were compared with sham operated control group. Gastrin levels in serum and antral tissue were measured and serum lipid concentrations were also measured. Gastrin levels of serum and tissue after bile deprivation were increased significantly compared with those of the controL At the end of 1st and 2nd week after bile deprivation, serum cholesterol and triglyceride contents were significantly lower han those of the control. By 4th week, there was no significant difference between two groups. Increases in serum and antral gastrin levels temporarily coincided well with decreases in serum lipid contents after bile deprivation. These results suggest that there is increase in biosynthesis and release of gastrin and decrease in fat absorption at early stage of bile deprivation.

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