• Title, Summary, Keyword: Soybean Embryo

Search Result 37, Processing Time 0.075 seconds

Effects of Soybean Embryo on Liver Protection and Lipid Metabolism of Alcohol-Fed Rats

  • Lee, Jae-Seok;Kim, Hye-Yun;Park, Kap-Joo;Lee, Hyung-Hoan
    • Food Science and Biotechnology
    • /
    • v.14 no.1
    • /
    • pp.102-107
    • /
    • 2005
  • In this study, the ameliorating effect of soybean embryos on the impact of alcohol consumption was investigated on rat hepatocytes and in reducing total serum cholesterol levels and total serum lipid levels. Liver histology and two clinically important enzyme markers, aspartate aminotransferase (AST) and alanine aminotransferase (ALT), of rats administered with both alcohol and soybean embryo were compared with a control group. The treatment regimen of soybean embryo significantly reduced the serum ALT and AST levels of the subjects, demonstrating the hepato-protective effects of soybean embryo. Electron microscopy indicated that the administration of soybean embryo preserved the important hepatocyte structures and prevented the presence of lipid droplets and secondary lysosomes. Furthermore, total cholesterol and total lipid levels were significantly reduced. These results indicate that treatment with soybean embryo can positively mediate the effects of alcohol on hepatocytes and general liver functions.

Physiological activity and efficacy of cosmetic products in bio-converted soybean embryo extract (생물전환 콩 배아추출물의 생리활성 및 화장품의 효능 연구)

  • Min, Kyung-Nam;Lee, Gun-Hee;Park, Sung-Jin;Choe, Tae-Boo
    • Journal of the Korea Convergence Society
    • /
    • v.10 no.3
    • /
    • pp.211-220
    • /
    • 2019
  • This study was to investigate the physiological activities of bioconversion soybean embryo extract and the effects of sleeping pack containing extract on skin. The aglycone isoflavones of the soybean embryo extract bioconversed with pectinase were identified by HPLC. in vitro tests showed that they were non-toxic, anti-inflammatory, anti-melanin and anti-aging activity. After 4 weeks of human body application with the sleeping pack using extract, the skin surface roughness and eye wrinkles were so improved by low stimulation that the sleeping pack containing bioconversion soybean embryo extract has a positive effect on the skin. We confirmed that the bioconversion soybean embryo extract was effective in enhancing the physiological activity and was useful as a cosmetic material of various formulations.

Transcriptional regulation of soybean ${\beta}-conglycinin$ gene expression. -(I) Identification of a soybean embryo factor interacting with upstream region of soybean ${\beta}-conglycinin$ gene- (대두 ${\beta}-conglycinin$ 유전자 발현의 전사 조절에 관한 연구 -(I) 대두 ${\beta}-conglycinin$ 유전자의 upstream 영역에 결합하는 대두 배 인자의 동정-)

  • Lee, Jeong-Yeon;Chung, Dong-Hyo;Kim, Woo-Yeon
    • Applied Biological Chemistry
    • /
    • v.36 no.6
    • /
    • pp.547-552
    • /
    • 1993
  • Soybean nuclear extracts and S-100 were prepared to examine the soybean embryo factors which bind to the upstream region of soybean ${\beta}-conglycinin$ ${\alpha}'$ subunit gene. SEF3(soybean embryo factor 3), which is presumed to be a trans-acting factor for the expression of the gene, was detected in gel mobility shift assay using the DNA probe containing two AACCCA hexanucleotides. DNA probe containing CATGCAT or AACACA was used to find any other soybean embryo factor interacting with the upstream region of ${\beta}-Conglycinin$ ${\alpha}'$ subunit gene. It was found that there was no common DNA binding protein detected both in nuclear extracts and S-100. The relative levels of SEF3 binding activity both in nuclear extracts and S-100 of maturing soybean seeds were determined. SEF3 activity of nuclear extracts was first detected around 20 days after pollination and significantly increased around 32 days after pollination.

  • PDF

Transcriptional regulation of soybean ${\beta}-conglycinin$ gene expression: -(II) Developmental change of soybean embryo factor 3 activity- (대두 ${\beta}-conglycinin$ 유전자 발현의 전사 조절에 관한 연구 -(II) 대두 발달과정 중의 대두 배 인자 3의 역가 변화-)

  • Lee, Kyung-Hoon;Chung, Dong-Hyo;Kim, Woo-Yeon
    • Applied Biological Chemistry
    • /
    • v.36 no.6
    • /
    • pp.553-556
    • /
    • 1993
  • Soybean nuclear extracts were prepared to examine the expression of SEF3 (soybean embryo factors 3), which binds to the upstream region of soybean ${\beta}-conglycinin$ ${\alpha}'$ subunit gene and is presumed to be a trans-acting factor for the expression of the gene. The relative levels of SEF3 binding activity in nuclear extracts of maturing soybean embryos were determined using the SE3 DNA probe containing two AACCCA hexanucleotides for gel mobility shift assay. The SEF3 activity increased in developing embryos from 16 to 32 days after pollination, whereas the mobility of the SE3-SE3-SEF3 complex decreased. The mobility of the complex was increased by the treatment of nuclear extracts with alkaline phosphatese, which could be inhibited by phosphate. Formation of the SE3-SEF3 complex was not affected by the binding buffer pH between 6.8 and 8.5.

  • PDF

Regeneration of Plants from EMS-treated Immature Embryo Cultures in Soybean [Glycine max(L.) Merr.]

  • Van, Kyu-Jung;Jang, Hyun-Ju;Jang, Young-Eun;Lee, Suk-Ha
    • Journal of Crop Science and Biotechnology
    • /
    • v.11 no.2
    • /
    • pp.119-126
    • /
    • 2008
  • Since somatic embryogenesis combined with ethylmethane sulfonate(EMS) treatments is the most efficient technique for mutagenesis, the embryogenic capacity of four soybean cultivars was evaluated at different EMS concentrations, treatment times, and preculture durations. Two to 4 mm long immature cotyledons were placed in induction medium after EMS treatment, and the numbers of somatic embryos formed per explant were counted four weeks after culture initiation. We observed genotypic differences in the efficiency of somatic embryogenesis from immature embryos among four cultivars treated with different concentrations of EMS for six hours. Cultivars, Sinpaldalkong 2 and Jack, displayed highly efficient somatic embryogenesis regardless of EMS concentration, whereas very low efficiency or no survival was observed in Jinju 1 and Iksannamulkong cultivars. Preculture duration did not influence the efficiency of somatic embryogenesis. Because Sinpaldalkong 2 exhibited the best somatic embryogenesis, much higher concentrations of EMS were used to test somatic embryo formation under different periods of time in this cultivar. Three and six hour treatments with both 1 and 2 mM EMS yielded higher embryo formation than longer periods of time. Increasing the time with embryos in 2 mM EMS caused a reduction in somatic embryogenesis in Sinpaldalkong 2, but many chlorophyll-deficient soybean variants were identified in the $M_1R_0$ and $M_2R_1$ generations. In addition to Jack, Sinpaldalkong 2 is a good genotype for plant regeneration from EMS-treated immature embryo cultures.

  • PDF

Structural Determination of Cerebrosides from Soybean Embryo by Mass Spectrometer (Mass Spectrometer를 이용한 대두 배아 출추 Cerebroside의 구조 분석)

  • Kim, Jung-Hun;Chang, Sug-Youn;Kim, Yeo-Kyung
    • Analytical Science and Technology
    • /
    • v.6 no.3
    • /
    • pp.335-343
    • /
    • 1993
  • The structure of cerebrosides from soybean embryo was determined using fast atom bombardment mass spectrometer (FAB-MS), gas chromatography mass spectrometer (GC-MS) and TLC. The components of cerebroside were determined by GC-MS after acid hydrolysis. The molecular weight distribution of cerebroside was measured by positive mode FAB-MS with LiOH saturated 3-nitrobenzylalcohol(3-NBA) matrix. Structures of individual components of complex mixtures can be determined easily by this process. The major constituent of soybean extracted cerebroside was determined as the glucoside of N-2'-hydroxypalmitoyl-sphingadienine.

  • PDF

Factors Affecting Somatic Embryogenesis from Immature Cotyledon of Soybean

  • Kim, Young Jin;Park, Tae Il;Kim, Hyun Soon;Park, Ho Ki;Chon, Sang Uk;Yun, Song Joong
    • Journal of Plant Biotechnology
    • /
    • v.6 no.1
    • /
    • pp.45-50
    • /
    • 2004
  • Somatic embryos were induced from immature cotyledons and cultured on a MS medium containing 40mg/L 2,4-D. The maximum induction of embryos was obtained from immature cotyledons in a size of 3-4mm, and the highest frequency was obtained in the induction medium at pH 7.0. For embryo development, embryogenic tissues were transferred to a MSM6AC and MSM6 media. Developing embryos were placed at 27$^{\circ}C$with dim light (20$\mu$$molm^{-2}$$s^{-1}$) provided by cool fluorescent tubes (3-D wavelength light is better than standard light). Somatic embryos were clearly developed from globular stage to cotyledonary stages. The color of embryo may be a useful parameter for estimation of embryo quality. When the embryo becomes mature, embryo will be ready for desiccation in order to induce roots and shoots of embryos.

Effect of the Extraction Method on the Soybean Embryo Factor 3 Activity (추출 방법에 따른 대두 배인자 3 역가)

  • Lee, Kyung-Hoon;Chung, Dong-Hyo;Kim, Seong-San;Song, Youn-Ho;Kim, Woo-Yeon
    • Applied Biological Chemistry
    • /
    • v.38 no.1
    • /
    • pp.63-66
    • /
    • 1995
  • Soybean nuclear extracts were prepared to detect SEF3(soybean embryo factor 3), which is presumed to be a trans-acting factor for the expression of the soybean ${\beta}-conglycinin\;{\alpha}'$ subunit gene. To increase the specific activity of DNA probe during labeling with $[{\alpha}-^{32}P]$dATP, dATP was added to a final concentration of 1.1 mM during the chase reaction. It results in approximately four-fold increase of specific activity of the DNA probe. Effects of several modifications in preparation of soybean nuclear extracts were examined. It was found that glycerol is effective to stabilize SEF3 during the preparation of nuclear extracts and polyethylenimine could be used to increase the specific activity of SEF3 in nuclear extracts.

  • PDF

Plant Regeneration of Soybean Cultivars via Somatic Embryogenesis

  • Kwang-Woong Lee
    • Journal of Plant Biology
    • /
    • v.37 no.3
    • /
    • pp.333-341
    • /
    • 1994
  • Effective plant regeneration from immature cotyledons of soybean [Glycine max (L.) Merr.] cultivars was achieved via somatic embryogenesis. Somatic embryogenesis was performed with the cotyledons of immature embryos 14-20 d after flowering. Immature cotyledons of cv. Whangkeum were placed abaxial or adaxial side down on modified MS medium containing 20mg/L 2,4-D. The greatest number of somatic embryos, 1.2 per cotyledon, was produced from those of 4.0-4.9 mm in length which had been placed abaxial side down. Among cvs. Pecking, Whangkeum and Baekwoon, Pecking had the highest embryo induction efficiency with 4.3 somatic embryos per cotyledon in 20mg/L 2,4-D treatment and with 1.0 embryo per cotyledon in 8mg/L NAA treatment. Germinable globular somatic embryos were induced with the highest efficiency, 27.6%, in 20mg/L 2,4-D and were proliferated efficiently on liquid medium containing 10mg/L 2,4-D. The globular somatic embryos developed into germinable mature somatic embryos on medium containing 10 $\mu$M CoCl2, 9% sucrose, and 0.5% activated charcoal. These mature somatic embryos germinated on hormone-free mediu. After transfer to the soil, regenerated plants with seeds were obtained.

  • PDF