• Title, Summary, Keyword: Spodoptera exigua

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Analysis of the Isozyme Loci of the Beet Armyworm, Spodoptera exigua(H bner) (파밤나방(Spodoptera exigua(H bner))의 동위효소 유전좌위 분석)

  • 김용균;김경성
    • Korean journal of applied entomology
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    • v.37 no.1
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    • pp.19-22
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    • 1998
  • Number of loci, allele frequencies, and subunit structures of 17 kinds of isozymes were analyzed in a laboratory strain of the beet armyworm, Spodoptera exigua (Hubner) to get genetic markers. These isozymes had 30 loci with 21 polymorphic (70.0% polymorphism); effective number of alleles per locus, average heterozygosity (H,), and inbreeding coefficient (F) were 2.52, 32.8%, and 2 1.0%, respectively.

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Comparative effects of nicotine and diazinon on larval mortality and activity of cytochrome P-450 monooxygenases in Helicoverpa assulta and Spodoptera exigua (담배나방과 파밤나방의 유충사망률과 cytochrome P-450 monooxygenases의 활성에 미치는 니코틴과 다이아지논의 영향)

  • 이정호;부경생
    • Korean journal of applied entomology
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    • v.32 no.2
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    • pp.225-235
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    • 1993
  • This study was carried out to investigate effects of diazinon, an organophosphate, and nicotine, a plant~originated toxic chemical, on larval mortality and activity of cytochrome P-45D monooxygenases, a major detoxifwation enzyme system, in Helicoverpa assulta and Spodoptera exigua. Diazinon treatment gave a higher mortaliLy to H. assulta larvae than S. exigua larvae. In contrast to the case of diazinon, nicotine caused a higher mortahty to S. exigua than to H. assulla larvae. It was partly due to the fact that nicotine induced the actiVIty of midgut cytochrome P-450 monooxygenases (MFO) more than diazinon did in If assulta larvae. When If. aSSlllta larvae were reared on their host p\am. NicotwlIa tuoocum leaves, other componentS were mostly metabolized with the exception of dietary nicotine.

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Identification of an entomopathogenic fungus, Nomuraea rileyi ANU101, infecting the beet armyworm, Spodoptera exigua, in Korea (국내 서식하는 파밤나방(Spodoptera exigua)에서 분리된 곤충병원성곰팡이 (Nomuraea rileyi ANU101)의 동정)

  • Hwang, Ji-Hyeun;Park, Bok-Ri;Kim, Geun-Seop;Lee, Sun-Gu;Kim, Yong-Gyun
    • The Korean Journal of Mycology
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    • v.37 no.2
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    • pp.139-143
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    • 2009
  • Several entomopathogenic fungi have been exploited to be developed into biological control agents in insect pest management. The beet armyworm, Spodoptera exigua, is a serious insect pest infesting various crops, but not effectively controlled by commercial chemical pesticides due to its high insecticide resistance. A fungal isolate was isolated from S. exigua larvae collected from cabbage field in Andong, Korea. The fungus could be cultured in potato dextrose agar. Larvae of S. exigua injected with the cultured conidia showed a potent entomopathogenicity. To identify the fungus isolate, its internal transcribed space (ITS) and surrounding partial 18S/28S regions were sequenced. The ITS sequence was highly matched (99%) to that of Nomuraea rileyi. Morphological characters of its hyphae and conidia were well fit to those of known N. rileyi. This study reports the first record of an entomopathogenic fungus, N. rileyi, in Korea.

Pathogenicity and Multiplication of Entomopathogenic Nematode, Steinernema carpocapsae Weiser, on Been Armyworm, Spodoptera exigua (Hubner) and Tobacco Cutworm, Spodoptera litura(Fabricius) (파밤나방(Spodoptera exigua (Hubner))과 담배거세미나방(Spodoptera litura(Fabricius))에 대한 곤충병원선충(Steinernema carpocapsae Weiser)의 감염력 및 증식력)

  • 한상찬;이성섭;김용균
    • Korean journal of applied entomology
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    • v.38 no.3
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    • pp.255-260
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    • 1999
  • Pathogenicity and multiplication of entomopathogenic nematode, Steinernema carpocapsae Weiser, ere analyzed in two insect hosts, Spodoptera exiga (Hubner) and Sp. litura(Fabricius). The estimated ${LC}^{50}s$(lethal concentration of the infective juveniles to kill 50% of the host insect population) were not different between tow insect species or among their developmental stages on the filter paper assay, thought the actual numbers of the infected nematodes were varied among them. The significant variation, however occurred in nematode multiplication between tow inset hosts. Temperature also gave sosignificatn effect on nematode multiplication rate that it took 6 days after infection at $25^{\circ}C$, but did 12 days at $20^{\circ}C$ to show the maximal nematode population peak($\approx$500,000 infective juveniles(IJ) in a 5th instar larva of Sp. litura and $\approx$-100,000 IJ in a 5th instar larva of Sp. exigua).

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Genetic Variation of the Beet Armyworm, Spodoptera exigua (Hubner), Populations in Korea Using Polymorphic Allozymes (다형 동위효소를 이용한 국내 파밤나방(Spodoptera exigua (Hubner)) 집단의 유전변이)

  • 강성영;김용균
    • Korean journal of applied entomology
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    • v.40 no.3
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    • pp.235-243
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    • 2001
  • Genetic variation of the beet armyworm, Spodoptera exigua (Hubner), was analyzed by polymorphic allozymes. Field populations were subdivided by different hosts, geographical locations, and seasons. Estimated average heterozygosity ($0.443\pm$0.013) indicated high genetic variation in all field populations of S. exigua. There were significant inbreeding effects deviated from Hardy-Weinberg equilibrium in each of subpopulations. These significant nonrandom matings were caused by within-subpopulations probably due to sampling errors, but not by mating isolation among subpopulations. Wrights ($F_{ST}$ ) and Neis (D) genetic distances indicated little genetic differentiation among subpopulations, though some southern local subpopulations (Haenam and Sachon) were relatively different of northern subpopulations (Andong and Kunwi). Estimated number of migrants per generation was 5.9 among host subpopulations, 10.6 among geographical subpopulations, and 31.8 among seasonal subpopulations. These genetic analyses suggest that Korean S. exigua subpopulations have little genetic differentiation mostly due to their significant migratory capacity.

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Biochemical Analysis of Physiological Stress Induced by High Frequency Sound Treatment in the Beet Armyworm, Spodoptera exigua (고주파 처리에 따른 파밤나방(Spodoptera exigua)의 생리적 스트레스의 생화학적 분석)

  • Kim, Yong-Gyun;Son, Ye-Rim;Seo, Sam-Yeol;Park, Bok-Ri;Park, Jung-A
    • Korean journal of applied entomology
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    • v.51 no.3
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    • pp.255-263
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    • 2012
  • High frequency sounds disrupt physiological processes, such as feeding behavior, development and immune responses of Spodoptera exigua. We analyzed high frequency sounds with respect to biochemical changes in S. exigua. High frequency sound (5,000 Hz, 95 dB) suppressed protein synthesis and secretion of midgut epithelium. It also significantly inhibited a digestive enzyme activity of phospholipase $A_2$. The gene expression of three different heat shock proteins and apolipophorin III was altered, particularly in midgut tissue in response to high frequency sound treatments. High frequency sound treatments significantly increased sugar and lipid levels in hemolymph plasma. These results suggest that high frequency sounds are a physiological stress that induces biochemical changes in S. exigua.

Attraction Effects of LED Trap to Spodoptera exigua Adults in the Greenhouse (시설재배지에서 LED 트랩을 이용한 파밤나방(Spodoptera exigua) 성충의 유인효과)

  • Kim, Min-Gi;Lee, Hoi-Seon
    • Journal of Applied Biological Chemistry
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    • v.55 no.4
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    • pp.273-275
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    • 2012
  • The attraction effects of light emitting diode (LED) trap to Spodoptera exigua adults were evaluated in greenhouse and compared with those of no light trap and black-light trap, which is typical used in commercial trap. 7 days later, white LED trap ($83.0{\pm}0.7$) was 2.4 times more attractive than black-light trap ($34.0{\pm}2.6$), whereas the no light trap was little attractive to S. exigua. These results suggest that white LED traps could be used for environmental insect control.

Leaf Spray Control Efficacy of the Entomopathogenic Nematode, Steinernema carpocapsae Weiser, Supplemented with the Selected Antidesiccant, Keltrol-F, on the Beet Armyworm, Spodoptera exigua(Hubner) (선발 내건제 Keltrol-F를 이용한 곤충병원선충(Steinernema carpocapsae Weiser)의 파밤나방(Spodoptera exigua(Hubner))에 대한 엽면살포 방제 효과)

  • 이성섭;김용균;한상찬
    • Korean journal of applied entomology
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    • v.39 no.3
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    • pp.199-205
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    • 2000
  • The field control efficacy of entomopathogenic nematode, Steinernema carpocapsae Weiser, was evaluated on the beet armyworm, Spodoptera exigua (Hiibner). The insect pest has been known to be a defoliator at the aerial part of the crop and difficult to be controlled effectively with most commercial chemical insecticides due to its insecticide resistance. To overcome the susceptibility of the nematodes to desiccation when they were applied by leaf spray in field condition, we screened several commercial antidesiccants (alkyl glucoside, CMC, glycerol, Keltrol-F, Kunipia-G, and Laponite LXG) optimal for survival of the nematodes. Keltrol-F (0.1 %) was selected as a candidate supplement for field application of the nematodes. Leaf spray of the nematodes at 5,000 infective juveniles/ml of distilled water containing 0.1 % Keltrol-F resulted in 87.7% control efficacy on the 3rd instar larvae of Sp. exigua.

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