• Title, Summary, Keyword: Streptococcus faecium

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Characteristics of the antibacterial substances produced by Lactobacillus casei subsp. and Streptococcus faecium (Lactobacillus casei subsp. 및 Streptococcus faecium이 생산한 항균성물질의 성상)

  • Kang, Kyoung-koo;Mah, Jum-sool
    • Korean Journal of Veterinary Research
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    • v.33 no.3
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    • pp.393-406
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    • 1993
  • Antibacterial substances produced by Lactobacillus casei subsp. and Streptococcus faecium were examined for its antibacterial effects against some pathogenic bacteria. They were partially purified with ammonium sulfate precipitation, methanol-acetone extraction, G-50 gel filtration and examined its characteristics. When L casei subsp. and Str faecium were cultivated in MRS broth, stationary phase of L casei is until 24 hours and Str faecium is 20 hours. pH change of the cultured medium was both decreased after 12 hours and then constant at pH 4.5~4.6 after 28 hours. MRS broth culture fluids of L casei subsp. and Str faecium appeared the antibacterial effects by the spot-on-the-lawn method against ETEC, Sal pullorum and Sta aureus. Culture filtrates of L casei subsp. and Str faecium also appeared the antibacterial effects by the disc diffusion method. Culture filtrates of L casei sub. rhamnosus 7469 produced 0.032M of lactic acid and 0.01M of acetic acid. Str faecium 27273 also produced 0.027M of lactic acid and 0.01M of acetic acid. Protein concentrations of culture filtrates produced by L casei sub rhamnosus 7469 and Str faecium 27273 was $495{\mu}g/m{\ell}$ and $594{\mu}g/m{\ell}$, respectively. Antibacterial substances which are partially purified by ammonum sulfate precipitation, methanol-acetone extraction and G-50 gel filtration inhibit the growth of ETEC, Sal pullorum and Sta aureus. Characteristics of purified antibacterial substances was examined. Its molecular weight was about 31Kd, stabilized at $100^{\circ}C/20min.$ and some of proteolytic enzyme treatment.

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Production of L-α-Glycerophosphate Oxidase by streptococcus faecium M74 · LC (Streptococcus faecium M74 · LC에 의한 L-α-Glycerophosphate Oxidase의 생산)

  • Lee, In-Ae;Lee, Eun-Sook;Lee, June-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.5
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    • pp.684-688
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    • 2003
  • The objective of this study was to search for the best strain as a source of L- $\alpha$-glycerophosphate oxidase (GPO) production and to establish the process technology for the purification of GPO on an industrial scale. The GPO was produced by culturing Streptococcus faecium, and purified by ammonium sulfate, DEAE-cellulose and hydroxyapatite chromatography. The relative activity was 60 units/L for 5. faecim ATCC 12755, 65 units/L for 5. faecium ATCC 19634, and 67 units/L for 5. faecium $M_{74}$.LC, respectively. The optimum condition for fermentation was $37^{\circ}C$ for temperature, 300 rpm for stir rate, 0.5 L/min for aeration rate and 17 hours. The main culture medium prepared by the modified AC medium. AC medium consists of 0.1% glucose, 0.2% glycerol, 1.0% tryptone and 1.0% yeast extract, 0.5% $K_2HP0_4$, pH 7.0. The GPO was purified by ammonium sulfate fractionation and ion exchange column chromatography, The yield and purity were 17.2% and 5.3 fold, respectively.

Isolation and Identification of Lactic Acid Bacteria for Preparation of Probiotics (생균제용 유산균의 분리 및 동정)

  • 이진규;김운태;이준호;유재홍;신원철
    • Microbiology and Biotechnology Letters
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    • v.19 no.5
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    • pp.429-432
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    • 1991
  • In order to prepare probiotics, a strain which has resistant against acid, bile salts and antibiotics was isolated from infant and anirnat feces. Among these isolated strains, TK- 19 showed a strong acid tolerance and growth at 500 $\mu g$/ml of Na-deoxycholate. Also strain TK-19 was resistant to kanarnycin (30 $\mu g$/ml), streptomycin (10 $\mu g$/ml), nisin (50 $\mu g$/ml) and tetracycline (10 $\mu g$/ml). The isolated strain TK-19 was identified as Streptococcus faeciam, and was named Streptococcus faeciurn TK-19.

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Antibacterial Activity of the Honey Bee Venom against Bacterial Mastitis Pathogens Infecting Dairy Cows

  • Han, Sang-Mi;Lee, Kwang-Gill;Yeo, Joo-Hong;Kweon, Hae-Yong;Kim, Bong-Soon;Kim, Jae-Myung;Baek, Ha-Ju;Kim, Soon-Tae
    • International Journal of Industrial Entomology
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    • v.14 no.2
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    • pp.137-142
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    • 2007
  • The antibacterial activity of Korean honey bee venom (KBV) was examined against seven major bacterial mastitis pathogens, Enterococcus faecium, Escherichia coli, Methicillin resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, Streptococcus intermedius, Streptococcus oralis and Streptococcus uberis isolated from infected mammary quarters of cows. Seven bacterial mastitis pathogens were studied for antibacterial activity of the KBV by disc diffusion assay, minimal inhibitory concentrations (MIC) and bacterial count in milk samples. The KBV showed activity against Ent. faecium, E. coli, MRSA, Staph. aureus, Strep. intermedius. The order of susceptibility of the bacteria against the KBV was Staph. aureus>MRSA>E. coli>Strep. intermedius>Ent. faecium>Strep. oralis>Strep. uberis. The MIC against Staph. aureus, MRSA and E. coli were stronger effect as compared with standard drug. The effect of the KBV ($100{\mu}g\;ml^{-1}$) on the viability of Ent. faecium, E. coli, MRSA, Staph. aureus, Strep. intermedius, Strep. oralis and Strep. uberis in milk differed significantly with each other within 12 h incubation period. The results indicate that KBV has significant antibacterial effects against major bacterial mastitis bacteria, Ent. faecium, E.coli, MRSA, Staph. aureus, Strep. intermedius. Results of the study indicate the potential use of KBV as alternative to antibiotic therapy. Further investigations are needed though to confirm its efficacy and its effects on the animals.

Simple and Rapid Extraction of a Bacteriocin Produced by Streptococcus parauberis Z49 from Fermented Cultures (발효배양액에서 Streptococcus parauberis Z49균주가 생산하는 Bacteriocin의 간편한 추출)

  • Park, Hong-Je;Khang, Yong-Ho
    • Korean Journal of Microbiology
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    • v.46 no.3
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    • pp.291-295
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    • 2010
  • A novel bacteriocin produced by Streptococcus parauberis Z49 strain was characterized and efficiently extracted from fermented cultures by use of aqueous two-phase systems. The nisin-like bacteriocin, which was active even after a heat treatment at $121^{\circ}C$ for 15 min and in the broad pH range from 2 to 12, showed inhibition of bacterial growth of Micrococcus luteus, Lactobacillus spp., Lactobacillus fermentum, Enterococcus faecium, Listereia monocytogenes, and Pseudomonas fluorescens. Optimal conditions of PEG 600/$Na_2SO_4$ aqueous two-phase systems for the simple and rapid extraction of a novel bacteriocin were determined to be PEG 600 15%, $Na_2SO_4$ 30%, and NaCl 8%, where the bacteriocin was concentrated in PEG layer.

Rapid and Simple Method for Isolating Plasmid DNA from Lactic acid Bacteria (유산균 Plasmid DNA의 신속 간편한 분리방법)

  • Bae, Hyung-Seok;Baek, Young-Jin;Kim, Young-Ki;Yoo, Min;Park, Moo-Young
    • Microbiology and Biotechnology Letters
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    • v.13 no.3
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    • pp.289-296
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    • 1985
  • A simple procedure for rapid isolation of plasmid DNA from lactobacillus species and streptococcus species is described. Lactic acid bacteria were cultured in the TCM broth containing 0.5% glycine and plasmid DNA was isolated from cells treated with mutanolysin by alkaline-detergent lysis method. Good results for releasing and isolating plasmid DNA from lactobacillus species were obtained by treatment of cells with 30$\mu\textrm{g}$ of mutanolysin per ml at 37$^{\circ}C$ for 5 to 10 min. For the streptococcus species, the optimum conditions were slightly different. The procedure could be used for rapid characterization of plasmid DNA in Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus helveticus, Streptococcus lactis, Streptococcus faecalis, Streptococcus faecium, and Streptococcus cremoris strains. Using this procedure, plasmids isolated from $1.5m\ell$ cultures could readily be visualized in agarose gel.

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Effect of Germanium-132 on the Growth of Lactic Acid Bacteria (젖산균의 성장에 미치는 Ge-132의 영향)

  • Park, Seok-Kyu;Lee, Sang-Won;Takafumi Kasumi
    • Korean Journal of Food Preservation
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    • v.6 no.4
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    • pp.506-513
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    • 1999
  • The growth of lactic acid bacteria was investigated in liquid broth medium containing organic germanium compound(Ge-132, carboxyethylgermanium sesquioxide) in the range of 0.01 to 10mg/ml. Most of all lactic acid bacteria tested were tolerant and could grow better to the high Ge-132 concentration. However, the growth of Leuconostoc mesenteroides and Pediococcus pentosaceus were inhibited in the presence of 10mg/m1 Ge-132. Among 22 strains tested, lactic acid bacteria that were grown to a high degree(about 2 times) by addition of Ge-132 (10mg/ml)were Lactococcus lactis, Lc. cremoris, Lc. diacetilactis, Enterococcus faecium and Streptococcus faecalis. The growth of these strains were markedly accelerated in the culture medium supplemented with 1.omg/ml Ge-132 The optimal concentration of glucose for growth of Lc. lactic was found to be high in medium containing Ge-132 as compared with the case of control. During cultivation viscosity in culture broths of Lc. lactis and Lc. cremoris was rapidly elevated by adding Ge-132 to medium containing high concentration of glucose, and then decreased after incubation of long time. However, in the cultivation of Lc. diacetilactis, E, faecium and S. faecalis, viscosity of culture broths was not increased, even though Ge-132 was shown to be an effective stimulant of growth.

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Antimicrobial Resistance Patterns of Vancomycin-Resistant Streptococcus equinus Isolated from Animal Foods and Epidemiological Typing of Resistant S. equinus by Microbial Uniprimer Kit

  • Choi, Sung-Sook;Lee, Jin-Woo;Kang, Byoung-Yong;Ha, Nam-Joo
    • Archives of Pharmacal Research
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    • v.26 no.8
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    • pp.638-643
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    • 2003
  • Raw milk samples, and cow and chicken intestines were tested to isolate vancomycin-resistant, gram-positive bacteria. From these samples, we isolated seven vancomycin-resistant Streptococcus equinus, two vancomycin-resistant viridans Streptococcus and two vancomycin-resistant Enterococcus faecium. The MICs of several antibiotics, including vancomycin, against these strains were tested. Seven isolates of S. equinus showed high level resistance to vancomycin and teicoplanin (>100 $\mu$ g/mL). The cell wall thickness of these strains was compared with that of the sensitive strain by TEM and no differences were obserbed between these strains. We compared the strains of vancomycin-resistant Streptococcus equinus using PCR with Microbial Uniprimer Kit. We concluded that it is necessary to combine other methods in order to cluster and identify all isolates of S. equinus.

Gliotoxin is Antibacterial to Drug-resistant Piscine Pathogens

  • Feng, Haoran;Liu, Sen;Su, Mingzhi;Kim, Eun La;Hong, Jongki;Jung, Jee H.
    • Natural Product Sciences
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    • v.24 no.4
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    • pp.225-228
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    • 2018
  • By activity-guided fractionation, gliotoxin was isolated as an antibacterial metabolite of the fungus Penicillium decumbens which was derived from the jellyfish Nemopilema nomurai. Gliotoxin was further evaluated for antibacterial activity against several piscine and human MDR (multidrug resistance) pathogens. Gliotoxin showed significant antibacterial activity against Gram-positive piscine pathogens such as Streptococcus iniae FP5228, Streptococcus iniae FP3187, Streptococcus parauberis FP3287, Streptococcus parauberis SPOF3K, S. parauberis KSP28, and Lactococcus garvieae FP5245. Gliotoxin showed strong activity especially against S. parauberis SPOF3K and S. iniae FP5228, which are resistant to oxytetracycline. It is noteworthy that gliotoxin effectively suppressed streptococci which are the major pathogens for piscine infection and mortality in aquaculture industry. Gliotoxin also showed strong antibacterial activity against multidrug- resistant human pathogens (MDR) including Enterococcus faecium 5270 and MRSA (methicillin-resistant Staphylococcus aureus) 3089.

Microbiological Studies of Foods - Microbial Load and Microflora of Dried File Fish Fillet - (식품(食品)의 미생물학적(微生物學的) 조사연구(調査硏究) - 시판(市販) 쥐치포의 미생물(微生物) 분포(分布) -)

  • Yoo, Jin Young;Chung, Dong Hyo;Kim, Jun Pyong
    • Microbiology and Biotechnology Letters
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    • v.13 no.1
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    • pp.1-5
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    • 1985
  • File fish fillets collected from markets were analyzed for their microbial load and microflora, resulting in the level of aerobic mesophiles ranged from $3.5{\times}10^3$ to $1.1{\times}10^8CFU/g$, total coliforms from less than 2.3 to $4.6{\times}10^5MPN/g$, fecal coliforms from 1ess than 2.3 to $1.1{\times}10^5MPN/g$, Enterobacteriaceae from $3{\times}10$ to $4.4{\times}10^5CFU/g$ and fecal Streptococci from $1.6{\times}10^2$ to $7.6{\times}10^6CFU/g$ having Staphylococcus hominis, Staphylococcus hemolyticus, Micrococcus varians, Micrococcus luteus and Streptococcus cremoris as constituent microorganisms of aerobic mesophiles, Escherichia coli and Proteus rettgeri of Family Enterobacteriaceae, and Streptococcus faecium and Streptococcus avium of fecal Streptococci.

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