• Title, Summary, Keyword: Streptococcus iniae

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Biological characteristics of Streptococcus iniae and Streptococcus parauberis isolated from cultured flounder, Paralichthys olivaceus, In Jeju (제주지역 양식 넙치(Paralichthys olivaceus)로부터 분리되는 Streptococcus iniae와 Streptococcus parauberis의 생물학적 특성)

  • Lee, Chang-Hoon;Kim, Pil-Youn;Ko, Chang-Sik;Oh, Duck-Chul;Kang, Bong-Jo
    • Journal of fish pathology
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    • v.20 no.1
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    • pp.33-40
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    • 2007
  • Biochemical characteristic of Streptococcus iniae and Streptococcus parauberis that are pathogens of streptococcosis of cultured flounder, Paralichthys olivaceus, in Jeju area was examined. The result of experiments on the grow according to temperatures showed that only S. parauberis grew at 10℃, the result of hemolysis test showed that only S. iniae bacteria showed β hemolysis. Only S. parauberis were positive in VP test and HIP test, both bacteria used α-D-glucose, D-mannose, D-psicose, D-trehalose, pyruvatic acid methyl ester, and glycerol as substrates. L-lactic acid was used only S. iniae bacteria, and β-methyl-D-glucosid was used only by S. parauberis. S. iniae exhibited acute infection patten, differently S. parauberis exhibited chronic infection patten in pathogenic test.

Phylogenetic Diversity and Comparison of RAPD Fingerprint Profile of Streptococcus iniae (Streptococcus iniae의 유전학적 다양성과 RAPD fingerprint profile의 비교)

  • Jeong Yong-Uk;Heo Moon-Soo
    • Journal of Life Science
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    • v.16 no.2
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    • pp.345-351
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    • 2006
  • Streptococcus sp. were isolated from cultured flounder (Paralichthys olivaceus) having Streptococcosis during 2004 to 2005 in Jeju Island. Ninety four Streptococcus iniae strains were isolated using biochemical test and multiplex PCR assay. Three genotypes (A, B, C-type) of S. iniae were appeared in the RAPD analysis and they showed international or local genetic polymorphism. Presently, S. iniae having A-type is a dominant S. iniae genotype in Jeju and showed band patterns at about 550, 850, 1000, 1300 and 2000 base pares. In this study, the reported P14 random primer, that used to distinguish serotypes of S. iniae could not be applied to distinguish Jeju island S. iniae's genetic polymorphism.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Streptococcus iniae shows potential as a subunit vaccine against various streptococcal species

  • Kim, Min Sun;Choi, Seung Hyuk;Kim, Ki Hong
    • Journal of fish pathology
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    • v.28 no.1
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    • pp.9-15
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    • 2015
  • The potential of Streptococcus iniae glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an antigen for a subunit vaccine was investigated using a zebrafish model. The recombinant S. iniae GAPDH was purified using His-tag column chromatography, and antisera against the recombinant GAPDH (rGAPDH) were produced by intraperitoneal immunization of rats. By immunization with S. iniae rGAPDH, the survival rates of zebrafish against an S. iniae challenge increased, suggesting that GAPDH would be an antigen capable of inducing protective immune responses in fish. Furthermore, we demonstrated using Western blotting, that the antisera against rGAPDH of S. iniae had cross-reactivity with GAPDH from Streptococcus parauberis and Lactococcus garviae, which are also culprits of streptococcosis in cultured fish in Korea. These results suggest that S. iniae GAPDH may be used as an antigen for the development of a subunit vaccine against streptococcosis caused by diverse cocci in cultured fish.

The Efficacy and Safety on Combination Vaccines : Edwardsiella tarda, Streptococcus iniae and S. parauberis, in Olive flounder, Paralichthys olivaceus (넙치, Paralichthys olivaceus에서 Edwardsiella tarda, Streptococcus iniae와 S. parauberis 혼합백신의 효능 및 안전성 연구)

  • Kwon, Mun-Gyeong;Hwang, Jee Youn;Jung, Sung Hee
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.6
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    • pp.1193-1200
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    • 2014
  • Edwardsiella tarda, Streptococcus iniae and S. parauberis are main bacterial pathogens in aquaculture farms of olive flounder, Paralichthys olivaceus. We have discussed the efficacy and safety of 3 type-combined vaccines (A: S. iniae 1mg + S. parauberis 1mg + E. tarda 1mg, B: S. iniae 1mg + S. parauberis 1mg + E. tarda 0.5mg, C: S. iniae 1.5mg + S. parauberis 1.5mg + E. tarda 1mg) through intraperitoneal injections in olive flounder. None of the vaccines gave rise to any signigicant side effects on histopathology and blood chemistry. The antibody titers and lysozyme activities of A type were higher than those of B, C and control. Four weeks after vaccination, RPS (relative percent survival rates) was 62.5~75% (A type), 50~66.7% (B type) and 55.6~62.5% (C type) respectively. As the results, the combined vaccines are possible to prevent edwardsiellosis and streptococcosis, and A type : S. iniae 1mg + S. parauberis 1mg + E. tarda 1mg, is the most effective out of them.

Use of 16S-23S rRNA Intergenic Spacer Region for identification in the fish pathogenic Streptococcus iniae (16S-23S rRNA Intergenic Spacer Region을 이용한 어류 병원성Streptococcus iniae의 분자생물학적 동정)

  • Jeong, Yong-Uk;Gang, Bong-Jo;Park, Geun-Tae;Heo, Mun-Su
    • Journal of fish pathology
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    • v.17 no.2
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    • pp.91-98
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    • 2004
  • This study was performed for the identification of Streptococcus sp. from cultured flounders (Paralichthys olivaceus) showing streptococcosis in the Jeju island. We isolated 10 strains of Streptococcus iniae from the cultured olive flounders with streptococcosis. Isolated strains were identified in S. iniae since they have formed the expected band through performing PCR assay using specific primers, Sin-1 (5'-CTAGAGTACACATGTACT(AGCT)AAG-3') and Sin-2 (5'-GGATTTTCCACTCCCATTAC-3'). In addition to 16S-23S rRNA intergenic spacers (ISR), operon structure of isolated strains showed that all strains had three 16S-23S rRNA ISR band patterns. The 16S-23S rRNA ISR sequence of isolated strains showed 96% sequence identity with S. iniae (GenBank accession number AF 048773). This paper is the first report that S. iniae is associated with streptococcosis of Olive flounder in Korea.

Comparative Study of Pathogenicity Following Single or Coinfection with Edwardsiella tarda and Streptococcus iniae in Olive Flounder, Paralichthys olivaceus (넙치, Paralichthys olivaceus에서 Edwardsiella tarda와 Streptococcus iniae 단독 또는 혼합감염의 병원성 비교 연구)

  • Kwon, Mun-Gyeong;Jung, Sung-Hee
    • Journal of Fisheries and Marine Sciences Education
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    • v.24 no.4
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    • pp.591-601
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    • 2012
  • Olive flounders, Paralichthys olivaceus, were intraperitoneally challenged with Edwardsiella tarda or Streptococcus iniae or both bacteria simultaneously. The pathogenicity was respectively compared with blood chemical using alanine aminotransferase (ALT), aspatate aminotransferase (AST), glucose and total protein, lysozyme activity, bacterial number of kidney and spleen, histopathological change, and cumulative mortality. The tested group of coinfection showed increased cumulative mortality, bacterial number of kidney and spleen, AST and histopathological change, but not in lysozyme activity compared with others. This study provides support for the conclusion that simultaneous infection with Edwardsiella tarda and Streptococcus iniae in a susceptible host results in higher pathogenicity, leading to the increment of bacterial number and the destruction of the internal organs.

In vitro combination effects of natural substances and antimicrobials against Edwardsiella tarda and Streptococcus iniae (In vitro에서 Edwardsiella tarda와 Streptococcus iniae에 대한 항균제와 천연 유래 항균물질의 병용효과)

  • Bak, Su-Jin;Kang, Bong-Jo;Park, Kwan Ha
    • Journal of fish pathology
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    • v.28 no.1
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    • pp.17-26
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    • 2015
  • The purpose of this study is to explore the possibility of essential oils and flavonoids improving the performance of antibiotics, proving a higher efficacy in the presence of natural substances against Edwardsiella tarda and Streptococcus iniae. Increased efficacy was observed in several cases: both essential oils and flavonoids increased antibacterial efficacy of oxytetracycline and flumequine against E. tarda by 2-fold; essential oils and flavonoids increased antibacterial efficacy of nalidixic acid against E. tarda by 4-fold; flavonoids increased antibacterial efficacy of josamycin against S. iniae by 4-fold. The results obtained in this study suggest that essential oils and flavonoids might be especially useful in increasing the antimicrobial activity of nalidixic acid and josamycin against E. tarda and S. iniae in fish. Natural compounds examined in this study could also be useful to help decrease the overuse of antibiotics in fish.

Studies on a Toxin/Antitoxin System in Streptococcus iniae (어류병원균 Streptococcus iniae의 toxin/antitoxin system에 대한 연구)

  • Yoon, Seongyong;Kim, Yeon Ha;Jeun, Moonjung;Seong, Minji;Yoo, Ah Young;Lee, Donghee;Moon, Ki Hwan;Kang, Ho Young
    • Journal of Life Science
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    • v.29 no.1
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    • pp.97-104
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    • 2019
  • Streptococcus iniae is a typical fish pathogen causing streptococcosis and it can also cause zoonotic infectious diseases. We studied S. iniae FP5228 isolated from infected olive flounder in Wando, Korea. In a study to find virulence factors in FP5228, we found that the number of live bacteria decreased dramatically in culture medium containing S. iniae FP5228 for more than 24 hr. This phenomenon was hypothesized to be related to Toxin ${\zeta}$ and Antitoxin ${\varepsilon}$ genes, components of the Toxin/ Antitoxin (TA) system on the 14 kb plasmid of FP5228. We used a protein overexpression system to identify it. The pBP1140 vector system was constructed to regulate the expression of Toxin ${\zeta}$ and Antitoxin ${\varepsilon}$ by IPTG and Arabinose. E. coli/pBP1140 strain grew slowly in early growth under toxin expression condition, and it was confirmed by microscopic observation that the strain became longer. S. iniae CK287, lacking a 14 kb plasmid of S. iniae FP5228 strain, was constructed. CK287 bacterial cells did not show rapid killing during culture, and the ability to produce biofilm was also decreased, and toxicity was weakened in cytotoxicity test and fish test. These results suggest that the TA system is involved in physiological regulation and expression of virulence factors in S. iniae FP5228.

Stability and efficacy of formalin-killed Streptococcus iniae vaccine for olive flounder, Paralichthys olivaceus (넙치에 대한 b-용혈성 연쇄구균 불활화백신의 안정성과 효능)

  • Cho, Mi-Young;Lee, Deok-Chan;Kim, Jin-Woo;Do, Jung-Wan;Lee, Joo-Seok;Kim, Myoung-Sug;Choi, Mi-Young;Kim, Yi-Cheong;Kang, Bo-Kyou;Yoon, Yong-Dhuk
    • Journal of fish pathology
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    • v.19 no.2
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    • pp.165-172
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    • 2006
  • This study was performed to verify the stability and efficacy of Streptococcus iniae formalin killed cells on storage at refrigerator temperature in olive flounder, Paralichthys olivaceus. The vaccines preserved for 6, 12 and 15months showed high stability of potency. The antibody titers and protection efficacy to challenge test were significantly higher in booster immunized groups than prime immunized groups during storage. Especially, above 60% of relative percent survival obtained at low antibody revel in prime immunized groups indicates that innate and non-specific immune system might act against the S. iniae challenged.

Effect of Dietary Inclusion of Yacon, Ginger and Blueberry on Growth, Feed Utilization, Serum Chemistry and Challenge Test against Streptococcus iniae of Juvenile Rockfish Sebastes schlegeli (배합사료내 야콘, 생강 및 블루베리 첨가에 따른 조피볼락 (Sebastes schlegeli) 치어의 성장, 혈액성상 및 Streptococcus iniae 감염에 미치는 영향)

  • Lee, Ki Wook;Kim, Hee Sung;Cho, Sung Hwoan;Park, Chan-Il;Ha, Min-Su
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.6
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    • pp.823-829
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    • 2016
  • Yacon (YC), ginger (GG) and blueberry (BB) were added to the diet of juvenile rockfish Sebastes schlegeli to investigate the effects of these substances on growth, feed utilization and resistance to Streptococcus iniae compared to feed additive ethoxyquin. A total of 480 fish were distributed in 12 tanks and four experimental diets [control (Con), with 0.01% ethoxyquin, and 1% YC, GG and BB diets] were provided in triplicate. After an 8-week feeding trial, 20 fish from each tank were infected with Streptococcus iniae. Fish fed the BB diet had higher weight gain than fish fed the GG and Con diets. Feed efficiency of fish fed the GG and YC diets was higher than that of fish fed the BB and Con diets. The protein efficiency ratio of fish fed the GG diet was higher than that of fish fed any other diet. The cumulative mortality 54 h after infection of fish fed the Con diet was higher than that of fish fed any other diet. These results indicate that YC, GG, and BB are effective dietary additive, not only improving growth performance and feed utilization but also lowering the mortality of juvenile rockfish exposed to S. iniae.