• Title, Summary, Keyword: Streptomyces sp

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Improvement of FK506 Production in the High-Yielding Strain Streptomyces sp. RM7011 by Engineering the Supply of Allylmalonyl-CoA Through a Combination of Genetic and Chemical Approach

  • Mo, SangJoon;Lee, Sung-Kwon;Jin, Ying-Yu;Suh, Joo-Won
    • Journal of Microbiology and Biotechnology
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    • v.26 no.2
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    • pp.233-240
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    • 2016
  • FK506, a widely used immunosuppressant, is a 23-membered polyketide macrolide that is produced by several Streptomyces species. FK506 high-yielding strain Streptomyces sp. RM7011 was developed from the discovered Streptomyces sp. KCCM 11116P by random mutagenesis in our previous study. The results of transcript expression analysis showed that the transcription levels of tcsA, B, C, and D were increased in Streptomyces sp. RM7011 by 2.1-, 3.1-, 3.3-, and 4.1-fold, respectively, compared with Streptomyces sp. KCCM 11116P. The overexpression of tcsABCD g enes in Streptomyces sp. RM7011 gave rise to approximately 2.5-fold (238.1 μg/ml) increase in the level of FK506 production compared with that of Streptomyces sp. RM7011. When vinyl pentanoate was added into the culture broth of Streptomyces sp. RM7011, the level of FK506 production was approximately 2.2-fold (207.7 μg/ml) higher than that of the unsupplemented fermentation. Furthermore, supplementing the culture broth of Streptomyces sp. RM7011 expressing tcsABCD genes with vinyl pentanoate resulted in an additional 1.7-fold improvement in the FK506 titer (498.1 μg/ml) compared with that observed under non-supplemented condition. Overall, the level of FK506 production was increased approximately 5.2-fold by engineering the supply of allylmalonyl-CoA in the high-yielding strain Streptomyces sp. RM7011, using a combination of overexpressing tcsABCD genes and adding vinyl pentanoate, as compared with Streptomyces sp. RM7011 (95.3 μg/ml). Moreover, among the three precursors analyzed, pentanoate was the most effective precursor, supporting the highest titer of FK506 in the FK506 high-yielding strain Streptomyces sp. RM7011.

Turfgrass Probiotics Reduce Population of Large Patch Pathogen and Improve Growth of Zoysiagrass (유용미생물 처리에 따른 들잔디 재배지의 갈색퍼짐병 병원균 감소와 잔디생육 촉진 효과)

  • Bae, Eun-Ji;Cheon, Chang Wook;Hong, A-Reum;Lee, Kwang-Soo;Kwak, Youn-Sig
    • Weed & Turfgrass Science
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    • v.6 no.3
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    • pp.249-261
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    • 2017
  • To prevent large patch disease, caused by Rhizoctonia solani AG-2-2, in zoysiagrass a fungicide, Tebuconazole and three microbial agents Streptomyces sp. Burkholderia sp. and Streptomyces sp. S8 were applied in commercial turfgrass cultivation field in Sanchung, Gyeongnam, Korea. All treatments showed 50% reduced the pathogen population in thatch layer throughout the yearly cultivation period. Not only reduced the pathogen population, Tebuconazole, Streptomyces sp. Burkholderia sp. and Streptomyces sp. S8 treatment also enhanced turfgrass growth, chlorophyll and proline content. Malondialdehyde contents in each treatment was reduced from 6.2~28.9% when compared with the control. Taken together, reduction of pathogen population in soil lowered the disease incidence or severity, and allowed the turfgrass developed as normal condition. The results suggested that the selected microbial agents may use as biological control and growth promotion agents for the Zoysia turfgrass.

Formation of Glucose Isomerase from Alkalophilic Streptomyces sp. B-2 (호알칼리성 Streptomyces sp. B-2의 Glucose Isomerase 생성조건)

  • 이은숙
    • Journal of the East Asian Society of Dietary Life
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    • v.7 no.1
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    • pp.35-39
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    • 1997
  • Studies on the glucose isomerase produced by alkalophilic Streptomyces sp. B-2. Glucose Isomerase (E. C. 5.3.1.5) which reversibly catalyzes reaction between D-glucose and D-fructose was demonstrated in cell free extracts of alkalophilic Streptomyces sp. B-2 isolated form soil. The maximum enzyme activity was found at glucose concentration 4(g/$\ell$) , xylose concentration 6(g/$\ell$), magnesium ion 1.0(g/$\ell$), yeast extract concentration 2.0(g/$\ell$), peptone concentration 3(g/$\ell$).

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Biocontrol of Cabbage Clubroot by the Organic Fertilizer Using Streptomyces sp. AC-3. (Streptomyces sp. AC-3을 이용한 배추 무사마귀병의 생물학적 방제)

  • 주길재;김영목;김정웅;김원찬;이인구;최용화;김진호
    • Microbiology and Biotechnology Letters
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    • v.32 no.2
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    • pp.172-178
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    • 2004
  • This research is performed for a biological control of Chinese cabbage clubroot, we isolated an antagonistic bacterium AC-3 against Plasmodiophora sp., causal pathogens of cabbage clubroot. The isolated strain was identified as Streptomyces sp. by culture morphology, biochemical reactions, and homology research based on l6S rDNA sequences. Streptomyces sp. AC-3 produced chitinase (9.3 units/$m\ell$) in culture broth. So Plasmodiophora sp. mycelia changed abnonnal swelling, curling and branching mycelia by Streptomyces sp. AC-3 culture. In a field infected by Plasmodiophora sp., the treatment of a organic fertilizer added 2% Streptomyces sp. AC-3 microbial inoculant, it resulted in about 50% reducing the severity of cabbage clubroot significantly on cabbage plants compared with treated organic fertilizer plants. Additional disease such as sclerotinia rot, fusarium wilt and pythium rot were also significantly reduced by the treatment of the organic fertilizer added Streptomyces sp. AC-3 microbial inoculant.

Antioxidative Effects of Cultivation of Streptomyces sp. BH-405 Isolated from Marine Origin (해양에서 분리한 Streptomyces sp. BH-405 배양액의 항산화 효과)

  • 류병호;이영숙;양승택
    • KSBB Journal
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    • v.15 no.2
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    • pp.150-155
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    • 2000
  • Antioxidative activity of c비ture of Streptomyces sp. BH-405 was investigated. After removal of pellets of Streptomyces sp. B BH-405, antioxidative substances were is미ated and suc$\infty$sively purified from culture of Streptomyces sp. BH-405 by by thin | layer chromatography $\pi$LC) or silica gel column chromatography. The fraction 3 obtained from ethylether fractionation of the C culture appeared highest level of anti oxidative activity as determined by thiocyanate method. Band 2 obtained by further P purification of this fraction showed higher anti oxidation level than that of same concentration of dl- $\alpha$ -tocopherol, butylated h hydroxy anisole (BHA). The band 2 showed higher rate of 1, 1.diphenyl 2-picrylhydrazyl (DPPH) decolorization than dl-$\alpha$-tocopherol. In the rat liver microsomes, band 2 rapidly inhibited lipid peroxidation which was initiated enzymatically by r reduced nicotinamide adenine dinucleotide phosphate (NADPH) or non-enzymatically by Fenton’s reagent. Band 2 inhibited on | lipid peroxidation of mitochondria or the linoleic acid hydro peroxide induced peroxidation system. It is concluded that band 2 obtained by fractionation of Streptomyces sp. BH-405 cultivation contained antioxidants with the capacity to inhibit oxidative m modification.

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Isolation of a petunia cell growth inhibitor from Streptomyces sp. 9602 (Streptomyces sp. 9602 균주로부터 페튜니아 캘러스 생장억제물질의 분리)

  • 김명조;곽상수
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.3
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    • pp.149-152
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    • 1997
  • To search for a compound inhibiting the petunia callus growth from Streptomyces sp., we investigated the activity in the culture broth of 400 strains. The active compound was successively purified with solvent fractionation, silica gel column chromatography from Streptomyces sp. 9602 strain, and identified as 2, 5, 7-trihydroxy-3-(5'-hydroxyhexyl)-1, 4-naphthoquinone by 1H-NMR, EI-MS, IR and UV. It inhibited the callus growth of petunia by 50% at $32\mu\textrm{g}$/mL.

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Studies on the Cell Immobilization of Alkalophilic Streptomyces sp. B-2 for the Glucose Isomerization (포도당 이성화를 위한 Alkalophilic Streptomyces sp. B-2의 균체 고정화에 관한 연구)

  • 이은숙
    • The Korean Journal of Food And Nutrition
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    • v.11 no.3
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    • pp.319-322
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    • 1998
  • The whole cell of alkalophilic Streptomyces sp. B-2 which produce glucose isomerase was immobilized by entrapment method for the effective production of high fructose syrup. The highest immobilized activity was achieved when the enzyme was bound to 2% $textsc{k}$-carrageenan. Immobilized glucose isomerase the pH optimum was about pH 7.5~8.5. Immobilization of alkalophilic Streptomyces sp. B-2 on 2% $textsc{k}$-carrageenan at 7$0^{\circ}C$ showed an increase in glucose isomerase activity. GI activity of immobilized cells was maximum Co2+ concentration 10-3M, Mg2+ concentration 10-3M.

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A Gap Phase-Specific Inhibitor of the Mammalian Cell Cycle from Streptomyces sp. ZF10 (Streptomyces sp. ZF-10이 생산하는 세포주기 저해제)

  • ;;Hiroyuki Osada
    • Microbiology and Biotechnology Letters
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    • v.22 no.5
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    • pp.495-498
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    • 1994
  • Genistein, a inhibitor of the progression of G$_{1}$ and G$_{2}$ phase of the mammalian cell cycle, was discovered through a unique screening system, in which effects of microbial metabolites on the cycle progression of the cultured mouse mammalian carcinoma cell were monitored by flow cytometry. The inhibitor was extracted from the fermentation broth of Streptomyces sp. ZF10 with ethyl acetate, and purified by silica gel column chromatography and HPLC.

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Regulation of extracellular alkaline protease biosynthesis in a strain of streptomyces sp. (Streptomyces sp. 일주에서 균체외 호염기성 단백질분해 효소의 생합성 조절)

  • 신현승;이계준
    • Korean Journal of Microbiology
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    • v.24 no.1
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    • pp.32-37
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    • 1986
  • In fermentation studies it revealed that Streptomyces sp. SMF 3001 started to synthesize extracellular alkaline protease from early exponential phase of cell growth. The biosynthesis of the alkaline protease was greatly induced by skim milk as a sola nitrogen source and further stimulation was observed under inorganic sulphur limited culture. However, it was found that the biosynthesis was apparently repressed by $NH_4^+$ and free amino acids, specially by cysteine. It was considered that the strain SMF 301 of Streptomyces sp. would produce the alkaline protease for the uptake of sulphur compounds from protein contained in the culture broth.

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Control of Ginseng Damping-off by Streptomyces sp. A75 and A501 (Streptomyces sp. A75와 A501 균주의 인삼 잘록병에 대한 방제효과)

  • Lee, Sang Yeob;Song, Jaekyeong;Yun, Bong-Sik;Park, Kyeong hun;Kim, Jeong Jun;Han, Ji Hee
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.330-337
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    • 2016
  • Streptomyces sp. A75 and A501 inhibited the mycelial growth of pathogenic Rhizoctonia solani and Pythium sp., which cause the ginseng disease known as damping-off. Three methods were evaluated for the control of these pathogens, using a mixture of the culture broths from Streptomyces sp. A75 and A501. The methods tested were seed dipping with 50-fold diluted broth, drenching of soil with 100-fold diluted broth after sowing, and combined seed dipping and drenching. These methods reduced the incidence of ginseng damping-off caused by R. solani by 81.3%, 84.8%, and 32.2% and that caused by Pythium sp. by 51.0%, 52.1%, and 75.3%, respectively. Based on these results, the combination of seed dipping and soil drenching after sowing using a mixture of the culture broths from Streptomyces sp. A75 and A501 effectively reduced the incidence of damping-off in ginseng.