• Title, Summary, Keyword: Subculture

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The Influence of the Youth Culture on the Street Fashion - On the Focus in 1960s - (청소년(靑少年) 문화(文化)가 스트리트 패션에 미친 영향(影響) - 1960년대(年代)를 중심(中心)으로 -)

  • Kan, Moon-Ja
    • Journal of Fashion Business
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    • v.2 no.1
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    • pp.35-48
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    • 1998
  • The purpose of this paper is to examine the street fashion, which is influenced by the youth culture in 1960s. Youth culture is the youth subculture which appeared multiform in the 1960s. It tried to create a new culture by subverting the ruling class culture with their combative life style and peculiar appearance which are distinguished from mother culture. Youth cultures as youth subculture are symbolically expressed in their style which had strong characteristics of sub-fashion and anti-fashion, and which are different from mother fashion in shape. Therefore youth subculture fashion had an effect on street fashion in the 60s. The influence that the youth culture fashion had had on the street fashion is summarized as follows: Beats fashion was intelligency look on black dress, black polo-neck shirts, black trousers, black glasses and heavy black eyeliner. Mods fashion was neat style on Edwardian suit with narrow trousers, pointer-toeshoes and smooth croped hair. Folkis fashion was ethnic look, middle east asian look, peasant look, Indian style, south america look, gypsy style, natural materials, natural dying, tie-dye, beads and hand made ornaments. Rockers fashion was violence appearance categorized 'blue collar' on black leather jacket decorated with metal studs and hand-painted insignia, blue jeans and boots for motor bike. Swinging London & the Psychedelics fashion was blend mods look with pschedelic color and motive pattern. Hippie fashion was intermix pschedelic and natural or ethnic style. It was appear to be a meaningless, unpredictable assortment of diverse items and style. Faded and tattered jeans, army jackets, vintage hat, beads, embroidered headband, white sheets, diverse theatrical costumes, sandals or bare feet, granny dress, peasant blouse, blanket cape, tie-dye T-shirts and long hair. Greasers fashion was adorned black leather jacket with chains, fringing, badges, insignia and sleeves had been crudely ripped off. They came to be known wore jeans so soaked with oil. Skinheads fashion was aggressive working class identity with donkey jacket, jeans rolled up, collarless union shirts, heavy boots, braces and hair cropped short.

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Selection of High Anthocyanin-Producing Cells from Habituated Callus Derived from Purple Sweetpotato (자색고구마 습관성캘러스로부터 안토시아닌 고생합성 세포주 선발)

  • Park, Hye-Jeong;Kim, Yoon-Sil;Park, Hyeon-Yong
    • Journal of Plant Biotechnology
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    • v.34 no.1
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    • pp.19-24
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    • 2007
  • Callus induction using leaf of purple sweetpotato (PSP) was decreased when subcultured. So we selected habituated callus in MS medium supplemented with $1{\mu}M\;2,4-D$ (2,4-dichlorophenoxyacetic acid) after 6 months of cultures (without subculture). It grew faster and easier than any other callus. It was able to proliferate in MS hormone free solid and liquid medium without any growth regulators and subculture limits. During subculture in liquid medium, a purple mottled spot formed in one of habituated cell aggregates without any treatment. This purple cell aggregates were carefully separated from habituated cell aggregates, and then subcultured by selecting purple cell aggregates for more than 2 years to be isolated. The color value of the pigment extracted of culture was 1.0 mg/mL, which was close to that of a pigment extracted from storage root, which was 1.5 mg/mL. This purple cell aggregates could therefore be used for the industrial mass production of anthocyanin.

Instability of Anthocyanin Accumulation in Vitis vinifera L. var. Gamay Freaux Suspension Cultures

  • Qu Junge;Zhang Wei;Yu Xingju;Jin Meifang
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.2
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    • pp.155-161
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    • 2005
  • The inherent instability of metabolite production in plant cell culture-based bioprocessing is a major problem hindering its commercialization. To understand the extent and causes of this instability, this study was aimed at understanding the variability of anthocyanin accumulation during long-term subcultures, as well as within subculture batches, in Vitis vinifera cell cultures. Therefore, four cell line suspensions of Vitis vinifera L. var. Gamay Freaux, A, B, C and D, originated from the same callus by cell-aggregate cloning, were established with starting anthocyanin contents of $2.73\;\pm\;0.15,\;1.45\;\pm\;0.04,\;0.7\;\pm\;0.024\;and\;0.27\;\pm\;0.04$CV (Color Value)/g-FCW (fresh cell weight), respectively. During weekly subculturing of 33 batches over 8 months, the anthocyanin biosynthetic capacity was gradually lost at various rates, for all four cell lines, regardless of the significant difference in the starting anthocyanin content. Contrary to this general trend, a significant fluctuation in the anthocyanin content was observed, but with an irregular cyclic pattern. The variabilities in the anthocyanin content between the subcultures for the 33 batches, as represented by the variation coefficient (VC), were 58, 57, 54, and $84\%$ for V. vinifera cell lines A, B, C and D, respectively. Within one subculture, the VCs from 12 replicate flasks for each of 12 independent subcultures were averaged, and found to be $9.7\%$, ranging from 4 to $17\%$. High- and low-producing cell lines, VV05 and VV06, with 1.8-fold differences in their basal anthocyanin contents, exhibited different inducibilities to L-phenylalanine feeding, methyl jasmonate and light irradiation. The low-producing cell line showed greater potential in enhanced the anthocyanin production.

Factors Influencing Drinking of Employees: Focus on the White Collar Employees (직장인의 음주에 영향을 미치는 요인에 관한 연구: 사무직 직장인을 중심으로)

  • Kweon, Gu-Young
    • Korean Journal of Social Welfare
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    • v.57 no.2
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    • pp.93-118
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    • 2005
  • This research examined influence of job stress and drinking subculture on the drinking of white collar employees. The results are as follows: First, there are a higher percentage of population among white collar employees who hold drinking problems in terms of frequency of drinking, amount of drinking, frequency of binge drinking comparing with general populace. The results of research revealed that drinking problems of white collar employees is quite close to dangerous level. Second, job stress didn't display a consistent relationship with drinking and drinking problem, however, drinking subculture revealed that it was related with drinking and drinking problem exhibiting significant influence. And, the results of research didn't support tension reduction hypothesis, and it was identified that social learning theory is main factor that will explain drinking and drinking problem of white collar employees. Therefore, researcher suggested as follow; First, practitioners and researchers exert their efforts for studying about drinking in the relationship to white collar employees. They should also have more interests in the topic from a practical perspective. Second, suggest that EAPs be introduced in order to prevent white collar employees from drinking problem and to promote increase of entire welfare. Third, suggest that succeeding research endeavors be required, which shall embrace all of white & blue collar employees.

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Evaluation of Negative Results of BacT/Alert 3D Automated Blood Culture System

  • Kocoglu M. Esra;Bayram Aysen;Balcl Iclal
    • Journal of Microbiology
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    • v.43 no.3
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    • pp.257-259
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    • 2005
  • Although automated continuous-monitoring blood culture systems are both rapid and sensitive, false-positive and false-negative results still occur. The objective of this study, then, was to evaluate negative results occurring with BacT/Alert 3D blood culture systems. A total of 1032 samples were cultured with the BacT/Alert 3D automated blood culture system, using both aerobic (BPA) and anaerobic (BPN) media, and 128 of these samples yielded positive results. A total of 904 negative blood samples were then subcultured in $5\%$ sheep blood agar, eosin methylene blue, chocolate agar, and sabouraud-dextrose agar. Organisms growing on these subcultures were subsequently identified using both Vitek32 (bioMerieux, Durham, NC) and conventional methods. Twenty four $(2.6\%)$ of the 904 subcultures grew on the subculture media. The majority $(83.3\%)$ of these were determined to be gram-positive microorganisms. Fourteen $(58.3\%)$ were coagulase-negative staphylococci, two $(8.3\%)$ were Bacillus spp., one $(4.2\%)$ was Staphylococcus aureus, and one $(4.2\%)$ was identified as Enterococcus faecium. Streptococcus pneumoniae and Neisseria spp. were isolated together in two $(8.3\%)$ vials. Gram-negative microorganisms comprised $12.5\%$ of the subcultures, of which two $(8.3\%)$ were found to be Pseudomonas aeruginosa, and one $(4.2\%)$ was Pseudomonas fluorescens. The other isolate $(4.2\%)$ was identified as Candida albicans. We conclude that the subculture of negative results is valuable in the BacT/Alert 3D system, especially in situations in which only one set of blood cultures is taken.

Development of In vitro Systems for Heat Tolerance Screening and Tuberization of True Potato Seeds (감자의 耐暑性 器內檢定 및 眞正種子의 器內塊莖形成體系 開發)

  • 김현준;김화영;유승렬;김병현;김정간
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.2
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    • pp.71-75
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    • 1997
  • For the establishment of early selection of heat-tolerant clones through in vitro tuberization of true potato seeds, different temperature treatments for in vitro tuberization were investigated. The ratios of tuberization at 2$0^{\circ}C$ on var. Superior and DTO-33 treated with 5 mg/L of BAP and 500 mg/L of CCC, were 85% and 92%, respectively. At 3$0^{\circ}C$, the ratio of tuberization on DTO-33 was 37%, which revealed strongly heat-tolerant clone. In culture system of in vitro tuberization, the number of tubers per flask at 2$0^{\circ}C$ on non-subculture incubation was more than that on subculture incubation. The condition of non-subculture and short-day treatment for 4 weeks was good for production as 10.6 tubers per flask, which was very similar to that of long-day treatment. On the other hand, tuber diameter on long-day treatment was greater as 11.2 mm than on short-day treatment. Therefore, in vitro tuberization from true potato seeds could be induced under the condition of long-day treatment at darkness.

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Gamma Radiation-Induced Changes of Antioxidant Enzymes in Callus Cultures of Cassava(Manihot esculenta Crantz) (감마선에 의한 카사바 (Manihot esculenta Crantz) 배양세포의 항산화효소 활성 변화)

  • 이행순;유순희;권석윤;김재성;곽상수
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.1
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    • pp.53-58
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    • 1999
  • The gamma radiation-induced changes of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in callus cultures of cassava (Manihot esculenta Crantz) selected as a high yield of cell line for SOD were investigated. In normal cultures, the cell growth reached a maximum at 30 days after subculture (DAS), followed by a rapid decrease with further cultures. The SOD and POD specific activities (units/mg protein) showed the highest at the immediately after subculture and subsequently decreased to 20 DAS, and then increased to 30 DAS, whereas the CAT activity showed the lowest at just after subculture, and it continuously increased from 15 DAS to 30 DAS, showing a good correlation with the cell growth. Irradiation of gamma-ray of 50 and 70 Gy on 7 DAS inhibited significantly the cell growth by 50% and 80% at 14 days after treatment (DAT), respectively. In the cells irradiated with 70 Gy, SOD and POD specific activities increased by 4 and 2.5 folds at 14 DAT, respectively, whereas CAT activity was not affected. The results indicate that SOD and POD may be involved in the antioxidative mechanism in relation to oxidative stresses induced by subcultures and by gamma radiation in callus cultures of cassava.

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The Search of the Habitus Formation Process in Professional Football Club Supporters (프로축구 서포터즈의 아비투스 형성과정 탐색)

  • Oh, Byoung-Don;Yu, Young-Seol
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.8
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    • pp.3672-3681
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    • 2013
  • The purpose of this study was to analyze subculture of professional football supporters with a view of Bourdieu's Habitus theory. What sorts of mechanism were worked when supporters formed their Habitus. The methods adopted in the article was qualitative. The qualitative information on the nature of their fandom was gleaned from 'virtual participant observation' or 'interviewing' of professional football fans that participated in discussions on the internet and participant observation of professional fans. The intensity and criteria sampling method was used to select 6 supporters who had participated in the supporting activities for more than five years and played crucial roles in running their organization as officials. The textual analysis which is consisting of translation, coding, and processing was used to mean the identification and exegesis of contextualization cues that make a text meaningful to the professional football supporters. an intended audience. The findings of this study were that (1) the subculture variety of activities for players and coaching staffs were the fundamental factors when supporters formed their subculture. (2) the professional supporters became habitus through the progressively development of subcultures such as enthusiastic supporters, small meetings, and events relating to soccer players.

Effects of Korean Maritime Police Subculture on Organizational Conflict (해양경찰의 하위문화가 조직갈등에 미치는 영향)

  • Lim, You-Seok;Kim, Jong-Gil;You, Young-Hyun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.22 no.6
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    • pp.688-693
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    • 2016
  • In this study, various conflicts in the maritime police organization were diagnosed and police subculture, which is different from dominant mainstream maritime police culture, was concluded to cause conflicts. In addition, maritime police conflicts and conflicts due to the balance, confrontation, and contradiction between bureaucratic and democratic values in the maritime police organization itself are discussed. The results of analysis on the effects of Maritime police subculture on organizational conflict are as follows: First, the influence of cynicism on functional conflict was explored. The higher the level of lies of the persons under investigation, the more functional conflict appeared. Also, if a person showed a low level of credibility among the police, this also led to functional conflict. In addition, if an individual showed low levels of cooperation and credibility with the police, this resulted in hierarchical conflict. Second, the influence of machismo on functional conflict was also explored. It was found that female officers experienced conflicts because of poorer job performance compared to male counterparts. In hierarchical issues, female officers experienced conflict over the reduced scope of tasks assigned to them and lower job performance ability. Third, the effect of non-acceptance of change in functional conflict was not statistically significant. An aggressive attitude toward crime control and rejecting jobs unrelated to crime influenced functional conflict, and an aggressive attitude and performing jobs unrelated to crime lead to functional conflict.

In vitro Multiplication of Haloxylon recurvum (Moq.) - a Plant for Saline Soil Reclamation

  • Dagla Harchand R.;Shekhawat N.S.
    • Journal of Plant Biotechnology
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    • v.7 no.3
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    • pp.155-160
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    • 2005
  • Haloxylon recurvum (Locally known as Khar) is drought and salt tolerant plant of Thar Desert. This plant is a major biomass producer and has economic and ecological importance for the region. There is need for study on biology, propagation and genetic improvement for utilization of this plant for reclamation of saline soils. We report here on in vitro propagation of Haloxylon recurvum (Moq.) using nodal explant. Secretion of phenolic compound from explants was a major constraint for establishment of culture. This was checked by thorough washing and quick transfer of explant on fresh culture medium. Juvenile nodal explant with leaves was found suitable for culture establishment. Benzy-ladenine($4.0\;{\mu}M$) incorporated in Murashige and Skoog (MS) medium with additives (50 mg/L ascorbic acid and 25 mg/L each of adenine sulphate, arginine and citric acid) induced multiple shoots from nodal explant. Addition of $1.0\;{\mu}M$ naphthalene acetic acid (NAA) in combination with $4.0\;{\mu}M$ BAP improved the growth of axillary shoots. Further shoot amplification was achieved by repeated subculture of mother explants on fresh medium. Forty percent of the micropropagated shoots rooted on half-strength MS medium with $4.0\;{\mu}M$ indolebutyric acid (IBA) and 100 mg/L activated charcoal, at $28{\pm}2^{\circ}C$ and $60\%$ RH. Sixty percent of these plantlets were hardened in green house.