• Title, Summary, Keyword: Swine Tissues

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Screening of Specific Genes Expressed in the Swine Tissues and Development of a Functional cDNA Chip

  • Kim, Chul Wook;Chang, Kyu Tae;Hong, Yeon Hee;Kwon, Eun Jung;Jung, Won Yong;Cho, Kwang Keun;Chung, Ki Hwa;Kim, Byeong Woo;Lee, Jung Gyu;Yeo, Jung-Sou;Kang, Yang Su;Joo, Young Kuk
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.7
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    • pp.933-941
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    • 2005
  • To develop a functional cDNA chip, specific genes expressed in the tissues of swine Kagoshima Berkshire were screened. A total of 4,434 ESTs were obtained by constructing a cDNA library from total RNA isolated from the muscle and fat tissues, affirming their functions by investigating similarity of nucleotide sequences with the database at the NCBI. Among them, 1,230 ESTs were confirmed as novel genes, which, to date, have not been identified. Attaching the genes to a cDNA microarray slide revealed expression patterns of genes in muscle and fat according to the growth stages of swine. As specific genes expressed in the muscle tissues of swine with body weight of 30 kg, 60 genes including actin, myosin, tropomysin, transfer RNA-trp synthetase, Kel-like protein 23, KIAA0182 and COI, Foocen-m, etc were obtained. In addition, 18 novel genes were obtained. As specific genes expressed in fat tissues of swine with body weight of 30 kg, 47 genes including annexin II, Collagen, Fibronectin, Pleckstrin homology domain, serine protease, etc were obtained. 21 novel genes were also obtained. The genes specifically expressed in the muscle and fat tissues of swine affect contraction and relaxation of the muscle and the fat. However, studies on the expression mechanisms of the genes are insufficient. To reveal species of structural genes in swine muscle and fat tissue, interrelation studies in expression and function of genes by using the cDNA chip should be conducted.

Determination of florfenicol residues in swine tissues using high-performance liquid chromatography with ultraviolet photometric detector

  • Chae, Won-Seok;Yoo, Chang-Yeul;Tutkun, Lutfiye;Kim, Suk;Lee, Hu-Jang
    • Journal of Preventive Veterinary Medicine
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    • v.42 no.4
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    • pp.171-176
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    • 2018
  • A study of the tissue depletion of florfenicol (FFC) administered orally to pigs at a dose of 0.05 kg/ton feed for 7 days was performed. Sixteen healthy cross swine were administered with FFC. Four treated animals were arbitrarily selected to be sacrificed 1, 3 and 5 days after the end of treatment. FFC residue concentrations in muscle, liver, kidney, and fat were determined using high-performance liquid chromatography (HPLC) with ultraviolet photometric detector at 230 nm. The correlation coefficient ($R^2$) of the calibration curve for florfenicol amine (FFCa) was > 0.997 and the limits of detection and quantification were 0.012 and $0.040{\mu}g/mL$, respectively. Recovery rates in swine edible tissues ranged from 79.1 to 93.5%. In the FFC-treated group, FFC residues at 3 days post-treatment were below the maximum residue limits (MRLs) in muscle, kidney and fat, and those at 5 days post-administration were below the MRLs in all edible tissues. These results suggest that the withdrawal period of FFC after the drug treatment might be 5 days, which is a sufficient amount of time for reduction of the FFC residues below the MRLs in all edible tissues.

Studies on the detection of sulfonamide residues in swine edible tissues (돈육내 sulfonamides의 잔류물질 검출에 관한 연구)

  • Shin, Youn-kyung;Kim, Tae-jong;Yoon, Hwa-joong
    • Korean Journal of Veterinary Research
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    • v.34 no.4
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    • pp.843-850
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    • 1994
  • The study was carried out to determine the residues of sulfonamides in swine edible tissues with high performance thin layer chromatography. For this purpose, the Rf values of sulfonamides in various solvent systems and the recovery rate of sulfameathazine from sampike saples were obtained. Thirty-four samples collected from meat market in Seoul were analyzed. The results obtained from the present study were followings: 1. The average recovery rate of sulfamathazine residues from spiked tissues 0.05, 0.1, 0.5 and 1mg/kg sample weight was 85%. 2. Two of 34 samples of pork for domestic consumption were reported to have been exceeded 0.05 ppm in sulfamethazine residues degree. 3. On the basis of the results, the degree of residues of sulfamethazine in swine meat for domestic consumption is seemed not to be dangerous for public health.

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A Study on the Sulfametazine residues in Swine Tissues (일부지역 돼지장기 및 근육내 잔류설파메타진에 대한 조사연구)

  • 김영철;이용욱
    • Journal of Food Hygiene and Safety
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    • v.5 no.4
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    • pp.197-204
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    • 1990
  • This study was carried out to determine the sulfametazine residues in swine tissues. For this experiment, 22 samples of porks were collected at meat markets in Seoul, 21 samples of swine muscles for export were collected at slaughterhouses in Gyunggi areas, and 18 samples of swine livers, kidneys, and muscles were collected from 6 swine at slaugterhouse in Seoul from the end of August to the early of October and were analyzed by High Performance Liquid Chromatography (HPLC). the results obtained were summarized as follows. 1. The sulfametazine residues were liver > kidney > muscle, and among liver, kidney and muscle were very significantly different(p<0.005). 2. The sulfametazine residue in liver was very significantly higher than that in muscle (p<0.005), the sulfametazine residue in kidney was significantly higher than that in muscle (p<0.005), but, the sulfametazine residue in liver was not significantly higher than that in kidney(p<0.05). 3. the sulfametazine residues in swine muscles for export wer exhibited a tendency to lower in small breeding size farms. 4. the sulfametazine fesidue in one of 22 samples of porks for domestic consumption was exceeded 0.1 ppm.

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cDNA Microarray Analysis of the Gene Expression Profile of Swine Muscle

  • Kim, Chul Wook;Chang, Kyu Tae;Hong, Yeon Hee;Jung, Won Yong;Kwon, Eun Jung;Cho, Kwang Keun;Chung, Ki Hwa;Kim, Byeong Woo;Lee, Jung Gyu;Yeo, Jung Sou;Kang, Yang Su;Joo, Young Kuk
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.8
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    • pp.1080-1087
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    • 2005
  • By screening specific genes related to the muscle growth of swine using cDNA microarray technology, a total of 5 novel genes (GF (growth factor) I, II, III, IV and V) were identified. Results of southern blotting to investigate the number of copies of these genes in the genome of swine indicated that GF I, GF III, and GF V existed as one copy and GF II, and GF IV existed as more than two copies. It was suggested that there are many isoforms of these genes in the genome of swine. Also, results of northern blotting to investigate whether these genes were expressed in grown muscle, using GF I, III, and V indicated that all the genes were much more expressed in the muscle of swine with body weight of 90 kg. Expression patterns of these genes in other organs, namely muscle and propagation and fat tissues, were investigated by extracting RNA from the tissues. These genes were not expressed in the propagation and fat tissues, but were expressed in the muscle tissue. To determine the mechanism of muscle growth, further studies should be preceded using the 3 specific genes related to muscle growth, that is GF I, III, and V.

Comparative Studies on the Free Amino Acids in Hog Cholera Infected Swine Tissues (돈(豚)콜레라 바이러스 감염조직(感染組織)의 유리(遊離)아미노산(酸)에 관(關)한 비교연구(比較硏究))

  • Yong, Mahn Joong
    • Korean Journal of Veterinary Research
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    • v.6 no.1
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    • pp.31-36
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    • 1966
  • The free amino acid contents in several tissues of swine were analyzed qualitatively by means of two dimentional paper chromatography. The tissues used were liver, kidney and spleen that were obtained from normal, immunized and hog cholera infected swines. The results obtained are as follows: 1. Liver: 20 amino acids were detected in normal, 17 in immunized and 15 in infected swines. 2. Kidney: 16 amino acids were detected in normal, 13 in immunized and infected swines. 3. Spleen: 15 amino acids were detected in normal in immunized and 13 in infected swines. 4. Glutamic acid, leucine, serine and threonine were present in high concentration in all of the cases examined. 5. The free amino acids were appeard to be decreased in the infected tissues with hog cholera virus.

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Development of Nested RT-PCR for the Detection of Swine Hepatitis E virus in Formalin-fixed, Paraffin-embedded Tissues and Comparison with in situ Hybridization

  • Park, Jung-eun;Ha, Seung-kwon;Chae, Chan-hee
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • pp.26-26
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    • 2003
  • Hepatitis E virus (HEV) has been recognized as a major cause of enterically transmitted non-A, non-B hepatitis in many developing countries. The taxonomy of HEV is not clear and the virus remains unclassified. The objective of this study was to optimize conditions and procedures to detect swine HEV in formalin-fixed, paraffin-embedded tissues by nested RT-PCR and compare this detection method with in situ hybridization. (omitted)

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A study on determination of carbadox and olaquindox in swine tissues by matrix solid Phase disperse method" (MSPD 방법에 의한 돈육중 Carbadox와 Olaquindox 분석법 연구)

  • 황래홍;김영수;김기근
    • Korean Journal of Veterinary Service
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    • v.19 no.2
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    • pp.163-171
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    • 1996
  • This study was carried out to determination of carbadox and olaquindox residues in swine tissues by MSPD(matrix solid phase disperse)method. The results obtained were as follows ; 1. Optimal wavelengths of UV for carbadox and olaquindox were 310 and 370nm, respectively 2. Ethyl acetate-Acetonitrile(8:2) was found to be adequate as extractant in this method. 3. The average overall recovery of carbadox at the 0.01, 0.05, and 1.0PPM spike levels was 89. 2% and that of olaquindox was 89.9%, and the detection limits were 0.5ng for carbadox and olaquindox.

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Effects of Dietary ${\beta}$-Cyclodextrin on Plasma Lipid and Tissue Cholesterol Content in Swine

  • Park, B.S.;Jang, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.1
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    • pp.100-105
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    • 2007
  • This study examined the effects of dietary ${\beta}$-cyclodextrin (${\beta}CD$) on the cholesterol of blood and tissues of swine. Thirty six male castrated swine ($Landrace{\times}Yolkshire{\times}Duroc$) weighing 50 kg were randomly assigned to one of four dietary groups until their weight reached 110 kg. The groups were: basal diet without ${\beta}CD$ (control) and basal diets containing 1.5%, 3.0%, or 5.0% ${\beta}CD$. Diets and water were offered ad libitum. No significant difference was found between treatments in terms of feeding performance measured by daily intake, daily weight gain, and feed efficiency. Addition of ${\beta}CD$ to the diets significantly reduced total lipid, triglyceride and total cholesterol levels in swine blood, particularly in the group receiving 5.0% ${\beta}CD$, which showed decreases (p<0.05) of 21.9%, 55.6% and 27.7%, respectively. Cholesterol levels in back fat, loin, belly and ham portions of swine fed ${\beta}CD$ significantly differed (p<0.05) from controls, especially in the 5.0% ${\beta}CD$-fed group, with reductions of 26.0%, 27.5%, 17.9% and 18.3%, respectively. These results suggested that the addition of ${\beta}CD$ to the diet of swine could reduce their body cholesterol by decreasing the migration of cholesterol through the blood.

A case report of swine kidney worm infection in a wild feral pig (야생 멧돼지에서 돼지신충(swine kidney worm) 감염 예)

  • Suh, Myung-deuk;Shin, Gee-wook;Kim, Chong-sup;Kwak, Soo-dong;Kim, Soon-bok;Yeon, Seung-chan
    • Korean Journal of Veterinary Research
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    • v.42 no.1
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    • pp.109-113
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    • 2002
  • This is the first case report of Stephanurus dentatus infection of a feral pig in Korea. In late April, 2000, a weakened feral pig was caught by blow gun from a very low level mountain near the Gyeongsang National University. We autopsied the feral pig in the laboratory of veterinary anatomy at the College of Veterinary Medicine. A total of 27 adult parasites, 11 females and 16 males, and numerous eggs were observed from the cysts formed in the perirenal tissues and ureters. The average size of males was $25.1{\pm}3.2mm$ long and of the females was $34.2{\pm}2.9mm$. The worms were stout, the females being about 2mm broad, and the internal organs were partly visible through the cuticle. The shape of thin-shelled eggs found in the cysts of perirenal tissues and ureter was ellisoidal and oval, and measured $40{\sim}65{\times}90{\sim}115{\mu}m$. The adult parasites were found in cysts which varied from 0.6 to 4cm in diameter, each cyst usually containing a pair of adult worms embedded in green pus. The ureter was thickened and almost occluded, with consequent hydronephrosis.