• Title/Summary/Keyword: T-2HS-BSA

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Studies on development of ELISA Kits for T-2 toxin (T-2 독소의 측정을 위한 ELISA Kits의 개발에 관한 연구)

  • Yoon, Hwa-joong;Kim, Taejong;Lee, Sung-yun
    • Korean Journal of Veterinary Research
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    • v.37 no.3
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    • pp.613-618
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    • 1997
  • Direct competitive enzyme linked immunosorbent assay(ELISA) with monoclonal antibodies have been studied for quantitative determination of T-2 toxin from the mold corn. The T-2HS, T-2HS-BSA, T-2HS-HRP and monoclonal antibodies against T-2 toxin produced in the studies were qualified for quantitative ELISA test of T-2 toxin. The mean recovery rate from ground com spiked T-2 toxin was 83%. The meaning range of the T-2 test was 60ng to $2{\mu}g$. According to the recovery results with the com spiked T-2, the tests proved to be suitable in the screening of the moldy feed samples for the presence of T-2 toxin and will be able to become the basis of the ELISA test for the quantitative screening kits of T-2 toxin.

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