• Title, Summary, Keyword: TGEV

Search Result 30, Processing Time 0.03 seconds

Preventive Effects on Transmissible Gastroenteritis(TGE) Using by TGEV Antiserum I. Serological Results, RT-PCR for Fecal and Small Intestin, FA Test (항혈청 투여에 따른 돼지 전염성 위장염 예방효과 I. 혈청학적 결과, RT-PCR 검사, 형광항체검사)

  • Chi, Yong-Zhe;Han, Jeong-Hee;Kwon, Hyuk-Moo;Hahn, Tae-Wook;Jeong, Hyun-Kyu;Park, Bong-Kyun
    • Korean Journal of Veterinary Pathology
    • /
    • v.6 no.1
    • /
    • pp.11-18
    • /
    • 2002
  • The purpose of this study was to investigate to potective effects against transmissible gastyoenteritis virus (TGEV) infection in piglets by administration of the TGEV antiserum orally at 5 hrs, 24hrs and 36hrs after birth. five piglets administered the antiserum were experimentally infected with TGEV at four-day-old. Control group were four piglets infected with TGEV only. Serum antibody titers against TGEV were examined by serum neutralization(SN) test, dectection for TGEV or TGEV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the TGEV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of TGEV in feces and small intestines by RT- PCR were 24.5% and 20.0% in TGEV antiserum treated group and 44.0% and 75.0% in control group, respectively. 3 The detection rate of TGEV antigen in the small intestine by IFA were 26.7% in TGEV antiserum treated group and 75.0% in control group, respectively. It was concluded that oral administration of antiserum against TGEV to piglets was effective in preventing TGEV infection.

  • PDF

Preventive Effects on Transmissible Gastroenteritis Using by TGEV Antiserum II. Clinical Sign, Histopathological and Immunohistochemical Findings (항혈청 투여에 의한 돼지 전염성 위장염의 예방효과 II.임상검사, 병리조직학적 검사, 면역조직화학적 검사)

  • Chi, Yong-Zhe;Han, Jeong-Hee;Kwon, Hyuk-Moo;Jeong, Hyun-Kyu
    • Korean Journal of Veterinary Pathology
    • /
    • v.7 no.1
    • /
    • pp.43-54
    • /
    • 2003
  • The purpose of this study was to investigate protective effects against transmissible gastroenteritis virus (TGEV) infection in piglets by administration of the TGEV antiserum orally at 2hrs, 24hrs and 36hrs after birth. Five piglets administered with the TGEV antiserum were experimentally challenged with TGEV at four-day-old. Control group was four piglets challenged with TGEV only. Clinical signs and gross, histopathological and immunohistochemical findings were examined. In clinical signs, piglets of the control group appeared the typical signs such as severe watery diarrhea, depression and anorexia but piglets of the TGEV antiserum adminstered group recovered progressively. In clinical signs, piglets of the control group appeared the typical signs such as severe watery diarrhea, depression and anorexia but piglets of the TGEV antiserum adminstered group recovered progressively. In mortality, control group showed 75%, but TGEV antiserum adminstered group showed 20.0 %, respectively. In gross findings, piglets of the control group appeared the typical findings of congestion, distension of lumen, contaning curdes of undigested milk in stomach. But gross findings of piglets of the TGEV antiserum adminstered group appeared milder than them of control group. In histopathological findings, piglets of the control group appeared the typical findings of villous atrophy and fusion, congesion, exfoliation, vacuolation, squamation, loss of cilia and proliferation of crypt. But histopathological findings of piglets of the TGEV antiserum adminstered group appeared milder than them of control group. In immunohistochemical findings, piglets of the TGEV antiserum adminstered group showed more intensive in reaction for IgA and IgG than them of control group. The recation for IgA was stronger than that of IgG. It was concluded that oral administration of TGEV antiserum to piglets was effective to prevent TGEV infection and reduce their mortality.

  • PDF

Molecular biological characterization of transmissible gastroenteritis viruses isolated in Korea (돼지 전염성 위장염 바이러스(국내분리주)의 분자생물학적 특성 규명)

  • Kwon, Hyuk-moo;Pi, Jae-ho
    • Korean Journal of Veterinary Research
    • /
    • v.38 no.2
    • /
    • pp.304-313
    • /
    • 1998
  • Sixteen Korean field transmissible gastroenteritis viruses (TGEVs) were isolated using swine testicular cell (STC) and the genomic diversity of them was analyzed. All TGEV isolates produced a typical cytopathic effect in STC and were confirmed as TGEV by immunofluorescence assay using monoclonal antibody against TGEV and PCR using TGEV specific primers. RNAs from TGEV field isolates and vaccine TGEV were extracted and amplified by RT and PCR. The RT-PCR products were digested with selected restriction enzymes and analyzed RFLP patterns. The N-terminal end region of S gene and ORF 3 and 3-1 genes of TGEV amplified by TGEV specific primer pairs seemed to be conserved. Most specific variations were detected in S gene amplified by TGEV 4/6 primer pairs which includes antigenic sites A and D. When the PCR products were treated with Sau3AI and Ssp I, Bvac(vaccine strain), field isolates 133 and 347 were differentiated from Miller and Purdue types. In the case of D5 field isolates, it was classified into Purdue type by Sau 3AI but classified into independent TGEV by Ssp I. Two different TGEV strains from D2 sample were confirmed by plaque purification and RT-PCR-RFLP analysis. To investigate the change occurring in TGEV genome after serial passage, the TGEV P44 strain was passaged through STC. There were specific changes in S gene and a large deletion was observed in ORF 3 and 3-1 genes. These studies showed that a distinct difference in genome exists among TGEV field isolates.

  • PDF

The transmuted GEV distribution: properties and application

  • Otiniano, Cira E.G.;de Paiva, Bianca S.;Neto, Daniele S.B. Martins
    • Communications for Statistical Applications and Methods
    • /
    • v.26 no.3
    • /
    • pp.239-259
    • /
    • 2019
  • The transmuted generalized extreme value (TGEV) distribution was first introduced by Aryal and Tsokos (Nonlinear Analysis: Theory, Methods & Applications, 71, 401-407, 2009) and applied by Nascimento et al. (Hacettepe Journal of Mathematics and Statistics, 45, 1847-1864, 2016). However, they did not give explicit expressions for all the moments, tail behaviour, quantiles, survival and risk functions and order statistics. The TGEV distribution is a more flexible model than the simple GEV distribution to model extreme or rare events because the right tail of the TGEV is heavier than the GEV. In addition the TGEV distribution can adjusted various forms of asymmetry. In this article, explicit expressions for these measures of the TGEV are obtained. The tail behavior and the survival and risk functions were determined for positive gamma, the moments for nonzero gamma and the moment generating function for zero gamma. The performance of the maximum likelihood estimators (MLEs) of the TGEV parameters were tested through a series of Monte Carlo simulation experiments. In addition, the model was used to fit three real data sets related to financial returns.

Analysis of the spike glycoprotein gene and nonstructural protein gene of transmissible gastroenteritis virus using PCR and RFLP analysis (PCR과 RFLP분석을 이용한 transmissible gastroenteritis virus의 spike glycoprotein gene과 nonstructural protein gene의 분석)

  • Kwon, Hyuk-moo
    • Korean Journal of Veterinary Research
    • /
    • v.36 no.3
    • /
    • pp.627-633
    • /
    • 1996
  • To analyze the genomic diversity of transmissible gastroenteritis virus (TGEV), the N-terminal half of the spike (S) glycoprotein gene and nonstructural protein gene (open reading frames 3 and 3-1) were amplified by reverse transcriptase reaction and polymerase chain reaction (RT-PCR), and analyzed using restriction fragment length polymorphism (RFLP) patterns of the amplified DNA. In this study, TGEV Miller (M6) and Purdue (P115) strains were used as reference strains, and two vaccine strains (MSV and STC3) and four Korea isolates (P44, VRI-WP, VRI-41, and VRI-48) were analyzed. All TGEV strains were amplified with three TGEV primer pairs. Although there was some exception in RFLP analysis, this method differentiated TGEV strains into following groups : Miller group (M6 and MSV), Purdue group (PUS, STC3, P44, VRI-WP, VRI-41, and VRI-48). Using Sau3AI and SspI, VRI-48 was differentiated from the Miller and Purdue type viruses. The RT/PCR in conjuction with RFLP analysis was a rapid and valuable tool for differentiating several strains of TGEV. This study revealed the occurences of distinct difference in genome of TGEV strains.

  • PDF

Sequence of the spike gene containing antigenic sites A and D of transmissible gastroenteritis virus isolated in Korea (국내분리 돼지 전염성 위장염 바이러스의 antigenic site A와 D를 포함하는 spike gene의 염기서열 분석)

  • Kwon, Hyuk-moo;Pi, Jae-ho;Seong, Hwan-woo
    • Korean Journal of Veterinary Research
    • /
    • v.38 no.2
    • /
    • pp.319-327
    • /
    • 1998
  • The nucleotide sequences of spike (S) glycoprotein containing antigenic sites A and D of TGEV isolated in Korea were determined and compared with published sequences for TGEVs. The TGEV 133 and DAE5 strains had 97.40% nucleotide sequence similarity. The overall nucleotide sequence similarity of the 133 and DAE5 strains compared with other TGEV strains was between 96.86% and 99.15%. The similarity of the predicted amino acid sequence of the 133 and DAE5 strains was 94.93%. The TGEV 133 and DAE5 strains had 94.93-98.61% amino acid similarity with published sequences of other TGEV strains. The sequences of amino acid codons in the antigenic sites A and D were identical among all the viruses although there were several nucleotide changes in region containing antigenic sites A and D of Korean TGEV isolates. By phylogenetic analysis of the sequences, two Korean isolates 133 and DAE5 seemed to be derived from different lineages. These studies showed that a distinct difference in genome exists among TGEV field strains isolated in Korea.

  • PDF

Differentiation between Porcine Epidemic Diarrhea Virus and Transmissible Gastroenteritis Virus in Formalin-fixed Paraffin-embedded Tissues by Multiplex RT-nested PCR and Comparison with in situ Hybridization

  • Jung, Kwon-il;Kim, Jung-hyun;Chae, Chan-hee
    • Proceedings of the Korean Society of Veterinary Pathology Conference
    • /
    • /
    • pp.27-27
    • /
    • 2003
  • Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) infections are considered difficult to distinguish clinically and histopathologically. Prompt differentiation between PEDV- and TGEV-associated enteritis would greatly facilitate the management of disease in countries where PEDV and TGEV are epizootic. Rapid differential diagnosis and treatment are crucial to reducing mortality and morbidity from PEDV- and TGEV-induced enteritis in piglets. The objective for this study was to develop a protocol to differentiate between PEDV and TGEV directly from formalin-fixed, paraffin-embedded tissue, using a multiplex reverse transcription-nested polymerase chain reaction (RT-nPCR) assay. (omitted)

  • PDF

Improvement of Virus Productivity by Sodium Butyrate in the Production of Porcine Transmissible Gastroenteritis Virus Vaccine (Sodium butyrate에 의한 돼지 전염성 위장염 바이러스 백신의 생산성 향상)

  • Lee, Chang-Jin;Kim, Cheol-Min;Jeong, Yeon-Ho
    • KSBB Journal
    • /
    • v.26 no.2
    • /
    • pp.107-111
    • /
    • 2011
  • The essential operating parameters in virus vaccine production are multiplicity of infection (MOI), harvest time, and infection time. Stimulating agents also can be applied in order to improve vaccine productivity further. We investigated the optimum operating conditions in porcine transmissible gastroenteritis virus (TGEV) vaccine production and the applicability of sodium butyrate (NaBu) as a stimulating agents for the improvement of vaccine productivity. The optimum MOI, infection time, and harvest time for high production of TGEV by swine testicle (ST) cells were found to be 0.0001 pfu/cell, 3 day after cell inoculation, and 24 hpi, respectively. NaBu is known as a histone deacetylase inhibitor that has been widely used for the high expression of recombinant protein using mammalian cells and for the enhancement of virus propagation. So we tried to examine the potential of NaBu as a stimulating agent and to determine the optimum concentration by comparing TGEV titers with different range of NaBu concentration. TGEV titer with 5 mM NaBu was 1.5 times higher than control. Therefore, we concluded that NaBu can be a promising agent for stimulating various vaccine production including TGEV and the optimum NaBu concentration for TGEV production was determined to be 5 mM.

Sequence analysis and cDNA probe hybridization of the nucleocapsid(N) protein gene of transmissible gastroenteritis virus(TGEV) and porcine epidemic diarrhea virus(PEDV) (Transmissible gastroenteritis virus(TGEV)와 porcine epidemic diarrhea virus(PEDV)의 nucleocapsid(N) 단백질 유전자에 대한 염기서열 분석과 cDNA probe hybridization)

  • Park, Ji-yong;Kim, Chul-joong;Shin, Kwang-soon;Kim, Won-yong;Kang, Shien-young;Park, Yong-ho;Han, Hae-jung;Park, Yong-ha
    • Korean Journal of Veterinary Research
    • /
    • v.35 no.3
    • /
    • pp.515-530
    • /
    • 1995
  • Coronaviridae에 속하는 transmissible gastroenteritis virus(TGEV)와 porcine epidemic diarrhea virus(PEDV)를 specific하게 detection할 수 있는 방법을 개발하고자 본 연구를 수행하였다. 두 바이러스 모두 RNA 바이러스이기 때문에 reverse transcription-polymerase chain reaction(RT-PCR)으로 nucleocapsid(N) protein gene의 cDNA를 증폭시켰다. SmaI으로 처리한 pTZ19R에 ligation시킨 후 염기서열을 밝히고자 sequencing하였다. 각각의 prototype virus와 비교하여 상동성을 밝혔다. 두 바이러스에 대한 cDNA probe를 제작하여 Southern blot hybridization을 실시하였다. TGEV의 경우 백신주인 P45와 병독주인 Miller strain을 사용하였다. cDNA를 증폭시키기 위해 N1/N1R과 N2/N2R 두 가지 primer를 이용한 결과, N1/N1R primer의 경우 586bp 크기의 PCR product를 얻을 수 있었고, N2/N2R primers로 582bp의 cDNA를 증폭시킬 수 있었다. PEDV 실험을 위하여 PED 임상 증상을 나타내는 분변을 이용하여 RT-PCR을 실시하였다. P2/P2R primer로 753bp의 PCR product를 얻을 수 있었다. TGEV의 두 가지 strain의 N protein gene을 sequencing하여 prototype인 Purdue strain과 염기서열 상동성을 조사한 결과, 97%이상의 높은 homology를 나타내었다. PED-V 역시 N protein gene을 sequencing하여 CV777과 염기서열 상동성을 조사한 결과 97%이상의 homology로 PEDV임을 알 수 있었다. TGEV와 PEDV의 염기서열을 비교한 결과 29%의 낮은 homology를 관찰할 수 있었다. 두 가지 바이러스의 N protein gene에 대한 cDNA probe를 제작하여 Southern blot hybridization을 한 결과, 각 바이러스에 매우 특이적 반응을 나타내었다.

  • PDF

Protective effects of IgY against diarrhea in suckling piglets -I. Serological result, FA test and RT-PCR- (IgY 투여에 따른 포유자돈의 설사에 대한 예방효과 -I. 혈청학적 결과, 형광항체검사 및 RT-PCT 검사-)

  • Jin, Wen;Yoon, Byung-Il;Han, Jeong-Hee
    • Korean Journal of Veterinary Service
    • /
    • v.31 no.1
    • /
    • pp.101-111
    • /
    • 2008
  • The purpose of this study was to investigate the protective effects against porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) in suckling piglets by oral administration of IgY. Twenty piglets were divided into two groups with the same number: group I (treated with IgY) and group II (not treated). Group I was administerd orally with IgY for three days from one-day-old and experimentally challenged with PEDV and TGEV at four-day-old. The other was administered with saline solution and challenged with PEDV and TGEV at four-day-old. Serum antibody titers against PEDV and TGEV were examined by enzyme-linked immunosorbent assay (ELISA) and the detection of PEDV or TGEV antigen from feces and small intestines was performed by reverse transcription-polymerase chain reaction (RT-PCR) and indirect immunofluorescence (IFA). The antibody titers of the group I was higher than that of the other, and lasted at the end of experiment. In the detection tests of both virus from feces and small intestine, the rate of the group I was lower. Based on these results, oral administration of IgY may be effective to prevent the diarrhea caused by PEDV and TGEV.