• Title, Summary, Keyword: Tricalcium phosphate

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AN EXPERIMENTAL STUDY ON THE EFFECT OF TRICALICUM PHOSPHATE AND DURAPATITE UPON THE PULP IN DOGS' TEETH (Tricalcium phosphate와 Durapatite가 치수조직(齒髓組織)에 미치는 영향(影響)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Yang, Moon-Kyu
    • Restorative Dentistry and Endodontics
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    • v.9 no.1
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    • pp.7-14
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    • 1983
  • The purpose of this study was to investigate pulpal responses to tricalcium phosphate and durapatite which recently introduced as a bone substitute. Tricalcium phosphate and durapatite were placed on the amputated pulp tissue in the dog's teeth. Animals were sacrificed after 1, 2, 3 and 4 weeks and specimens were decalcified, embedding, sectioned and stained routinly. Microscopic examination reveals as follows; 1. Tricalcium phosphate: Severe inflammatory change was seen in the all cases and calcified masses were seen at 2 weeks. Calcified masses were enlarged according to the time elapsed. 2. Durapatite: Severe inflammatory change and pus cavities were found at 1 week. There was no evidence of healthy cell component in the remaining pulp tissue and degenerative change was obvious at 2, 3 and 4 weeks. 3. Dentin bridge was not formed in the both cases. In the case of tricalcium phosphate osteoblasts were observed unevenly around the calcified masses which were composed of hematophilic substance in central portion and eosinophilic substance in peripheral region of the masses.

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Effect of Silicon contained Coralline Hydroxyapatite and Beta Tricalcium Phosphate in human intrabony defects (성인 치조골 내 결손부에서 Silicon contained Coralline Hydroxyapatite와 Beta Tricalcium Phosphate 합성제재의 효과에 대한 임상적 고찰)

  • Jang, Yong-Ju;Kim, Yong-Tae;Park, Jung-Chul;Kim, Chang-Sung;Choi, Seong-Ho;Kim, Chong-Kwan
    • The journal of the Korean dental association
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    • v.47 no.9
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    • pp.596-606
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    • 2009
  • Aim : The ultimate goal of periodontal treatment is regeneration of periodontium that have been lost due to inflammatory periodontal disease. Recently, Silicon contained Coralline Hydroxyapatite and Beta Tricalcium Phosphate bone substitute have been introduced to achieve periodontal regeneration. The purpose of this study is to evaluate the effect of the Silicon contained Coralline Hydroxyapatite and Beta Tricalcium Phosphate(BoneMedik-$DM^{(R)}$, Meta Biomed Co., Ltd. Oksan, Korea) on periodontal intrabony defects. Methods and materials : Clinical effects of Silicon contained Coralline Hydroxyapatite and Beta Tricalcium Phosphate implantation in intrabony defects were evaluated 6 months after surgery in Sixty-one intrabony defects from Fourty-six patients with chronic periodontitis. Twenty-nine experimental defects in twenty-five patients received the Silicon contained Coralline Hydroxyapatite and Beta Tricalcium Phosphate(test group), while Thirty-Three defects in twenty-one patients were treated with flap procedure only( control group). Comparative observation were done for preoperative and postoperative differences between control and experimental clinical parameters,-clinical attachment 10ss(CAL), probing depth(PD), bone probing depth(BPD), gingi val recession. Results : Postoperative improvements in CAL, PD, BPD were observed in both test and control groups(P<0.0l). However, the improvements in CAL, PD, BPD of the test group were significantly greater than control group. Conclusion : Healing of the both groups were uneventful during experimental periods. Use of Silicon contained Coralline Hydroxyapatite and Beta Tricalcium Phosphate in a flap operation resulted in significantly greater improvements in CAL, PD, and BPD over flap operation alone. Silicon contained Coralline Hydroxyapatite and Beta Tricalcium Phosphate will be good bone substitute materials for treatment of intrabony defects.

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Solubilization of Insoluble Phosphates by Penicillium sp. GL-101 Isolated from Soil (토양에서 분리한 Penicillium sp. GL-101에 의한 난용성 인산염의 가용화)

  • Choi, Myoung-Chul;Chung, Jong-Bae;Sa, Tong-Min;Lim, Sun-Uk;Kang, Sun-Chul
    • Applied Biological Chemistry
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    • v.40 no.4
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    • pp.329-333
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    • 1997
  • Phosphate solubilizing microorganisms (1,000 bacteria and 200 fungi) were isolated from soil around Kyungnam and Kyungbook regions using potato dextrose agar-calcium phosphate medium. A fungus with the greatest phosphate solubilizing activity was selected and identified to Penicillium sp. GL-101, based on the morphological characteristics of conidiophore and conidia; flask shape of phialide, simple branching type of conidiophore, and columnar shape of conidial head, in malt extract agar and potato dextrose agar media. The optimum temperature and initial pH to solubilize rock phosphate in potato dextrose broth-rock phosphate medium were $25^{\circ}C$ and pH 7.5, respectively. In these optimum conditions, phosphate solubilizing activities of Penicillium sp. GL-101 against four types of insoluble phosphate: tricalcium-phosphate, aluminium phosphate, hydroxyapatite and rock phosphate, were quantitatively determined. As results, this fungus highly discharged free phosphates to the culture broth with the concentrations of 1,152 ppm against tricalcium-phosphate, 565 ppm against rock phosphate, 292 ppm against aluminium phosphate, and 217 ppm against hydroxyapatite, respectively.

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Solubilization of Insoluble Phosphates by Aspergillus sp. PS-104 Isolated from Soil (토양에서 분리한 Aspergillus sp. PS-104 균주에 의한 난용성 인산염 분해)

  • Kang, Sun-Chul;Shin, Seung-Yong
    • Korean Journal of Environmental Agriculture
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    • v.26 no.1
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    • pp.36-41
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    • 2007
  • Phosphate-solubilizing microorganisms were isolated from soil around Kyungnam and Kyungbook regions using potato dextrose agar-calcium phosphate medium. A fungus with the greatest phosphate-solubilizing activity was selected and identified to Aspergillus sp. PS-104, based on the morphological characteristics of conidiophore and conidia; unbranching type of conidiophore, terminally swelling of conidiophore and septate of mycelium, in malt extract agar and potato dextrose agar media. The optimum temperature and initial pH to solubilize rock phosphate in potato dextrose broth-rock phosphate medium were $30^{\circ}C$ and pH 7.0, respectively. In these optimum conditions, phosphate-solubilizing activities of Aspergillus sp. PS-104 against four twos of insoluble phosphate, tricalcium phosphate, aluminium phosphate, hydroxyapatite and rock phosphate, were quantitatively determined. As result, the maximum phosphate-solubilizing activity was obtained with tricalcium-phosphate (1,900 ppm) while minimum activity was obtained with hydroxyapatite (320 ppm). Futhermore, phosphate-solubilizing activity of Aspergillus sp. PS-104 was found higher when treated with nitrates as compared to the ammonium salts as a nitrogen sources.

The Effect of $\beta$-Tricalcium Phosphate and Deproteinized Bovine Bone on Bone Formation in the Defects of Rat Calvaria (흰쥐 두개골 결손부에서 베타-트리칼슘 인산염과 탈단백우골의 골형성 효과)

  • Jung, Seung-Gon;Park, Hong-Ju;Ryu, Sun-Youl
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.32 no.4
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    • pp.313-323
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    • 2010
  • Purpose: This study was conducted to evaluate the effect of beta-tricalcium phosphate (Cerasorb$^{(R)}$, Germany) and deproteinized bovine bone (Bio-Oss$^{(R)}$, Switzerland) grafted to the defect of rat calvaria artificially created and the effect of use of absorbable membrane (BioMesh$^{(R)}$, Korea) on new bone formation. Materials and Methods: Transosseous circular calvarial defects with diameters of 5 mm were prepared in the both parietal bone of 30 rats. In the control group I, no specific treatment was done on the defects. In the control group II, the defects were covered with absorbable membrane. In the experimental group I, deproteinized bovine bone was grafted without absorbable membrane; in the experimental group II, deproteinized bovine bone was grafted with absorbable membrane; in the experimental group III, beta-tricalcium phosphate was grafted without absorbable membrane; in the experimental group IV, beta-tricalcium phosphate was grafted with absorbable membrane. The animals were sacrificed after 3 weeks and 6 weeks respectively, and histologic and histomorphometric evaluations were performed. Results: Compare to the control groups, the experimental groups showed more newly formed bone. Between the experimental groups, beta-tricalcium phosphate showed more resorption than deproteinized bovine bone. Stabilization of grafted material and interception of the soft tissue invasion was observed in the specimen treated with membrane. There was no statistical difference between the experimental group I, III and experimental group II, IV classified by graft material, but statistically significant increase in the amount of newly formed bone was observed in the experimental group I, II and II, IV classified by the use of membrane (P<0.05). Conclusion: Both beta-tricalcium phosphate and deproteinized bovine bone showed similar osteoconductibility, but beta-tricalcium phosphate is thought to be closer to ideal synthetic graft material because it showed higher resorption rate in vivo. Increased new bone formation can be expected in bone graft with use of membrane.

Inorganic Phosphate Solubilization by Immobilized Pantoea agglomerans under in vitro Conditions (고정화된 Pantoea agglomerans에 의한 난용성 인산의 가용화)

  • Kim, Eun-Hee;Park, Sung-Ae;Park, Myoung-Su;Yang, Jin-chul;Madhaiyan, Munusamy;Seshadri, Sundaram;Sa, Tong-Min
    • Korean Journal of Soil Science and Fertilizer
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    • v.37 no.1
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    • pp.36-40
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    • 2004
  • It is now widely accepted that immobilized microbial cells can overcome some of the problems associated with microbial survival stability, efficacy, storage, transportation and ease of application in agricultural environments. Pantoea agglomerans, a phosphate solubilizing bacterium, was immobilized in alginate, agar and gelatin carriers. All the three immobilfized carriers with bacterial cells of P. agglomerans were compared for solubilization of tricalcium phosphate in pure liquid cultures. While alginate beads were tested for phosphate solubilization on alternate days up to five days, agar beads and gelatin cubes were subjected for one time phosphate solubilization analysis after seven days. Both alginate and agar immobilized cells of P. agglomerans exhibited higher efficiency in increasing the solubilizaliun of tricalcium phosphate than gelatin immobilized cells. The culture filtrate of alginate bead inoculation treatment registered a rapid increase in soluble phosphate concentration upon incubation. A corresponding decrease in the pH of the medium was also observed in all the treatments.

A Study on Binary System of Calcium Phosphate-Kaolin, -Quartz, -Feldspar and Limestone (Calcium Phosphate와 Kaolin, Quartz, Feldspar, Limestone과의 이성분계 소성에 관한 연구)

  • 이응상;이성희
    • Journal of the Korean Ceramic Society
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    • v.25 no.5
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    • pp.437-442
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    • 1988
  • We have suffered number of problems in supplying bone ash for bone china bodies as raw materals, because of its impurity and quantity. To reduce these problems, we have synthesized tricalcium phosphate that was reacted by H2PO4 and CaCO3 ; 3Ca(OH)2+2H3PO4longrightarrowCa3(PO4)2+6H2O. Therefore, we have studied solid reactions of synthesized tricalcium phosphate withkaoline, limestone, feldspar and silica, respectively.

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Isolation and Cultural Characteristics of a Phosphate-solubilizing Fungus, Penicillium sp. PS-113 (인산가용화 사상균, Penicillium sp. PS-113 균주의 분리 및 배양특성)

  • 강선철;최명철
    • KSBB Journal
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    • v.13 no.5
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    • pp.497-501
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    • 1998
  • A fungus with high phosphate solubilizing activites was isolated from soil using potato dextrose agar-calcium phosphate medium and identified to Penicillium sp. PS-113, based on the morphological characteristics of conidiophore and conidia; flask shape of phialide, simple branching type of conidiophore, and columnar shape of conidial head, in malt extract agar and potato dextrose agar media. The optimum temperature ad initial pH to solubilize rock phosphate in potato dextrose broth-rock phosphate medium were 30$^{\circ}C$ and pH 8.0, respectively. In these conditions phosphate solubilizing activities of Penicillium sp. PS-113 against four types of insoluble phosphate like tricalcium-phosphate, aluminium phosphate, hydroxyapatite and rock phosphate were quantitatively determined. As results, this fungus highly produced free phosphates to the culture broth with the concentrations of 1,283 ppm against tricalcium-phosphate, 585 ppm against rock phosphate, 528 ppm against aluminium phosphate, and 242 ppm against hydroxyapatite, respectively.

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Fabrication of Hydroxyapatite Whiskers by Hydrolysis of α-TCP (α-TCP의 가수분해에 의한 수산화아파타이트 휘스커의 제조)

  • 백동주;양태영;이윤복;윤석영;박홍채
    • Journal of the Korean Ceramic Society
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    • v.40 no.6
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    • pp.608-614
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    • 2003
  • Well developed hydroxyapatite whiskers (length 5 ${\mu}{\textrm}{m}$, diameter 0.5 ${\mu}{\textrm}{m}$) have been synthesized by the hydrolysis reaction of $\alpha$-tricalcium phosphate ($\alpha$-Ca$_3$(PO$_4$)$_2$) under pH 9.1 at 9$0^{\circ}C$ for 6 h. The effect of reaction conditions (temperature, time, pH) on the conversion of $\alpha$-tricalcium phosphate to hydroxyapatite was examined. In addition, the hydroryapatite was characterized in terms of microstructure, composition and thermal stability using XRD, SEM, ICP, and TGA instruments.