• Title, Summary, Keyword: Tricalcium phosphate

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Polyvinylidene fluoride/hydroxyapatite/β-tricalcium phosphate multifunctional biocomposite: Potentialities for bone tissue engineering

  • Bonadio, Taiana G.M.;Freitas, Valdirlei F.;Tominaga, Tania T.;Miyahara, Ricardo Y.;Rosso, Jaciele M.;Cotica, Luiz F.;Baesso, Mauro L.;Weinand, Wilson R.;Santos, Ivair A.;Guo, Ruyan;Bhalla, Amar S.
    • Current Applied Physics
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    • v.17 no.5
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    • pp.767-773
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    • 2017
  • Bioactive ferroelectric composites based on polyvinylidene fluoride, hydroxyapatite and ${\beta}-tricalcium$ phosphate have been synthesized and their structural, microstructural, bioactive, and ferroelectric properties are characterized. Structural and FTIR investigations showed the presence of the polar polyvinylidene fluoride (${\beta}_2$) phase, while ferroelectric characterizations revealed remnant polarizations and coercive field, around $0.04{\mu}C/cm^2$ and 28 kV/cm, respectively, for these biocompatible samples. Structural and microstructural analysis of samples previously immersed in simulated body fluid for 7 days revealed a large apatite phase growth ($1.45{\mu}m$) on the composites' surfaces as a strong indication of their elevated bioactivity and potentialities for bone tissue engineering.

Chemical solution derived hydroxyapatite films on Si substrates (화학 용액법으로 Si 기판 위에 제조한 하이드록시아파타이트 박막에 관한 연구)

  • 송종은;류현욱;신종윤;김병훈;김윤호;임용무
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.9 no.6
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    • pp.570-573
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    • 1999
  • Hydroxyapatite films were prepared on Si(100) substrates by using a sol-gel method with calcium nitrate and phosphoric acid as starting materials. Precursor sols were spin-coated onto the substrates and prefired at $500^{\circ}C$ for 10 min in air. Formation of the hydroxypatite structure was confirmed in the sample annealed at $500^{\circ}C$ by the X-ray diffraction $\theta$-2$\theta$ scans and a tricalcium phosphate phase was observed in the samples annealed at both temperature regions of $500^{\circ}C$~$700^{\circ}C$ and $900^{\circ}C$. From the results of Fourier transform infrared spectroscopy, the change of a carbon content and improvement of crystallinity have been discussed as a function of increase of annealing temperature.

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Microsotructure Designed Porous Apatite Ceramics Prepared by Hydrothermal Method

  • Ioku, Koji;Fukuhara, Michiko;Fujimori, Hirotaka;Goto, Seishi
    • The Korean Journal of Ceramics
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    • v.5 no.2
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    • pp.162-164
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    • 1999
  • Microsotructure designed porous ceramics of calcium hydroxyapatite $(Ca_{10}(PO_4)_6(OH)_2)$ were prepared by hydrothermal method. The particle size, shape, and the micro-pore size of the porous hydroxyapatite ceramics could becontrolled. The hydroxyapatite was non-stoichiometric apatite with calcium deficient compositions (Ca/P ratio < 1.67). The composition of non-stoichiometric hydroxyapatite ceramics could be controlled from 1.50 to 1.63 in Ca/P ratio. The hydroxyapatite ceramics preparedc at $105^{\circ}C$ under the saturated vapor pressure for 20h were composed of rod-shaped crystals with about 10$\mu\textrm{m}$ in length with the mean aspect ratio of 40. The porous ceramics of calcium deficient hydroxyapatite had about 45% porosity with the inter-connecting pore structure. Porous hydroxyapatite ceramics prepared hydrothermally had the compressive strength of about 10 to 30 MPa. In addition, porous ceramics of $\beta$-tricalcium phosphate ($\beta-Ca_3(PO_4)_2$) were prepared from the calcium deficient hydroxyapatite.

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A histomorphometric study of dental implants with different surface characteristics

  • Pak, Hyun-Soon;Yeo, In-Sung;Yang, Jae-Ho
    • The Journal of Advanced Prosthodontics
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    • v.2 no.4
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    • pp.142-147
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    • 2010
  • PURPOSE. One of the major keys to achieve successful osseointegration of the implant is its surface properties. The aim of this study was to investigate the bone response to dental implants with different surface characteristics using the rabbit tibia model. Tricalcium phosphate (TCP) coated, anodic oxidized and turned (control) surfaces were compared. MATERIALS AND METHODS. Seventy two implants were placed in the tibia of eighteen rabbits. Nine rabbits were sacrificed at 3 weeks of healing and the remaining nine were sacrificed at 6 weeks of healing. The bone-to-implant contact (BIC) and the bone volume density (BVD) were assessed by light microscope after 3 and 6 weeks of healing. RESULTS. Statistical analysis showed that no significant differences in the BIC and BVD were observed between the different implant surfaces and the control group at 3 weeks and 6 weeks of healing. Data also suggested that the BVD of all the surfaces showed significant difference at 3 and 6 weeks. CONCLUSION. The present study has showed that osseointegration occurred in all investigated types of surface-treated implants. In the current study all of the threads of the implants were observed to calculate BIC and BVD values (instead of choosing some of the threads from the bone cortex for example), which didn't make BIC or BVD percentage values better than in the control group, therefore the clinical relevance of these results remains to be shown.

Fabrication of Blended PCL/β-TCP Scaffolds by Mixture Ratio of β-TCP using Polymer Deposition System (폴리머 적층 시스템을 이용한 β-TCP 혼합 비율에 따른 PCL/β-TCP 인공지지체의 제작)

  • Ha, Seong-Woo;Kim, Jong Young
    • Journal of the Korean Society for Precision Engineering
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    • v.31 no.9
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    • pp.791-797
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    • 2014
  • Abstract Scaffold used as a carrier of the cell has been actively conducted using plenty of technology in tissue engineering. ${\beta}$-tricalcium phosphate (${\beta}$-TCP) material has shown good biocompatibility and osteoconductive ability when it was implanted as a bone graft substitute in osseous defect in human and animal studies for bone regeneration. In this study, we fabricated the blended polycaprolactone (PCL) and ${\beta}$-TCP scaffold by the polymer deposition system (PDS). The PCL/${\beta}$-TCP scaffold was fabricated at a temperature of $110^{\circ}C$, pressure of 650 kPa, and scan velocity of 100 mm/sec. The Overall geometry and size of the scaffold were fixed circle type with a diameter of 10 mm and a height of 4 mm. PCL/${\beta}$-TCP scaffold was observed by scanning electron microscopy. Cell attachment and proliferation of the scaffold containing 30 wt% ${\beta}$-TCP was superior to those containing 10 wt% and 20 wt% ${\beta}$-TCP.

Solid State Sintering of Calcium Phosphate Ceramic Composites and Their Cellular Response

  • Cho, Yeong-Cheol;Kong, Young-Min;Riu, Doh-Hyung
    • Korean Journal of Metals and Materials
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    • v.50 no.9
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    • pp.691-695
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    • 2012
  • Calcium phosphate ceramic composites, consisting of hydroxyapatite(HA) and tricalcium phosphate (TCP), were fabricated by solid state sintering in order to investigate the effect of their initial compositions on microstructural evolutions and biocompatibility. All the sintered calcium phosphate ceramics exhibited almost full densification, while the grain growth of the composites increased with an increasing TCP content in the green body. The TCP phase transformed into a Ca-deficient HA phase during sintering via the diffusion of calcium ions from the HA phase into the TCP phase. The phases formed in the composites significantly affected the biocompatibility of the composites. The HA-matrix ceramic composites with TCP had a better cellular response than the pure HA ceramics, presumably due to the newly formed Ca-deficient HA.

Numerical analysis of fracture mechanisms for porous calcium phosphate (다공성 칼슘포스파이트에 대한 파괴분석)

  • Park, Jin-Hong;Bae, Ji-Yong;Shin, Jae-Bum;Jeon, In-Su
    • Proceedings of the KSME Conference
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    • pp.1301-1302
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    • 2008
  • In this study, the fracture strength for fracture mechanism porous calcium phosphate made from sintered with ${\beta}$-tricalcium phosphate obtained by wet precipitation procedure is analyzed using finite element method and experiment measurement. First, three $3{\times}3{\times}3mm^3$ and $5{\times}5{\times}5mm^3$ specimens are prepared and tomographic images of one $5{\times}5{\times}5mm^3$ specimen are obtained by micro focus X-ray CT. The compression tests using the specimens are carried out to measure the elastic modulus and fracture strength to analyze the fracture mechanism of porous calcium phosphate specimen. The tomographic images are reconstructed by 3D reconstruction program. The finite elements are directly built up in the reconstructed specimen. The numerical simulation for the compression tests is performed using the element. The mechanism of calcium phosphate of simulation are obtained by the compression tests using there cylindric specimen of height 19.5 mm and diameter 10 mm. From the results, the applicability of porous calcium phosphate is evaluated to care fracture and vacant bone of a patient as the reinforcement material.

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Characterization of Phosphate Solubilizing Yeasts from Korean Traditional Fermented Foods (한국 전통 발효식품에서 분리한 인산가용화 효모의 특성)

  • Park, In-Cheol;Kim, Jeong-Seon;Jung, Joo Ae;Yoo, Jae-Hong
    • The Korean Journal of Mycology
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    • v.41 no.4
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    • pp.218-224
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    • 2013
  • Of 1,100 yeast strains which were isolated from various Korean fermented foods, screened for phosphate solubilization, five strains showed the ability to solubilize tricalcium phosphate. The 26S rDNA domain D1-D2 sequence analysis revealed the identification of strain Y393 and Y524 as Pichia anomala (99.8 and 100% identity, respectively), Y669 as Pichia farinosa (100% identity), Y901 as Candida versatilis (100% identity), and Y1101 as Pichia subpelliculosa (100% identity). All the phosphate solubilizing strains showed mesophilic characteristics. The temperature range for growth of 4 strains was $20{\sim}35^{\circ}C$ and P. farinosa Y669 was able to grow up to $45^{\circ}C$. The strain C. versatilis Y907 was able to grow at pH range of 5.0~6.0 and showed halophilic characteristics with tolerance to 15% of NaCl concentration. The Phosphate solubilizing yeast strains were survived well in bed soil for 8 weeks which were maintained densities of $10^7{\sim}10^8$ cfu/g. The highest phosphate solubilizing activity was observed in P. subpelliculosa Y1101. It solubilized 697.2 ug/mL of phosphorus from tricalcium phosphate with decrease in pH from 6.8 to 4.37 after 11 days of inoculation.

Effects of various toothpastes on remineralization of white spot lesions

  • Jo, Su-Yeon;Chong, Hyun-Jeong;Lee, Eon-Hwa;Chang, Na-Young;Chae, Jong-Moon;Cho, Jin-Hyoung;Kim, Sang-Cheol;Kang, Kyung-Hwa
    • The korean journal of orthodontics
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    • v.44 no.3
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    • pp.113-118
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    • 2014
  • Objective: The purpose of this in vitro study was to examine the effects of fluoridated, casein phosphopeptide-amorphous calcium phosphate complex (CPP-ACP)-containing, and functionalized ${\beta}$-tricalcium phosphate (fTCP)-containing toothpastes on remineralization of white spot lesions (WSLs) by using Quantitative light-induced fluorescence (QLF-D) Biluminator$^{TM}$ 2. Methods: Forty-eight premolars, extracted for orthodontic reasons from 12 patients, with artificially induced WSLs were randomly and equally assigned to four treatment groups: fluoride (1,000 ppm), CPP-ACP, fTCP (with sodium fluoride), and control (deionized water) groups. Specimens were treated twice daily for 2 weeks and stored in saliva solution (1:1 mixture of artificial and human stimulated saliva) otherwise. QLF-D Biluminator$^{TM}$ 2 was used to measure changes in fluorescence, indicating alterations in the mineral contents of the WSLs, immediately before and after the 2 weeks of treatment. Results: Fluorescence greatly increased in the fTCP and CPP-ACP groups compared with the fluoride and control groups, which did not show significant differences. Conclusions: fTCP- and CPP-ACP-containing toothpastes seem to be more effective in reducing WSLs than 1,000-ppm fluoride-containing toothpastes.

Characteristic Analysis and Fabrication of Bioceramic Scaffold using Mixing Ratios of TCP/HA by Fused Deposition Modeling (압출 적층 조형 기술을 이용한 TCP/HA 의 혼합비율에 따른 바이오 세라믹 인공지지체의 제작 및 특성 연구)

  • Sa, Min-Woo;Kim, Jong Young
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.38 no.11
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    • pp.1273-1281
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    • 2014
  • Tissue engineering is an emerging research field that has the potential to restore, regenerate and repair damaged bone tissue and organs. Tricalcium phosphate and hydroxyapatite biomaterials-based calcium phosphate are excellent materials that have both osteoconduction and biocompatibility for bone tissue regeneration. In this study, solution structures were successfully fabricated using a fused deposition modeling system based on deposition and heating devices. The morphology characteristics of the bioceramic scaffolds sintered at a temperature of $1,300^{\circ}C$ were analyzed by scanning electron microscopy. The effects of various blended TCP/HA ratio on the microstructure and shrinkage were studied. The mechanical properties of the scaffolds were measured using a compression testing machine from stress-strain curves on the crosshead velocity of 1 mm/min. The fabricated scaffolds were evaluated by cell proliferation tests of MG-63 cells. The results of this study suggest that the blended TCP(75 wt%)/HA(25 wt%) scaffold is an appropriate scaffold for bone tissue regeneration.