• Title, Summary, Keyword: Vibrio Parahaemolyticus

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Application of the β-lactamase (VPA0477) Gene for the Detection of Vibrio parahaemolyticus by Polymerase Chain Reaction (β-Lactamase (VPA0477) 유전자를 표적으로 Polymerase chain reaction에 의한 장염비브리오(Vibrio parahaemolyticus)의 검출)

  • Park, Kwon-Sam
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.6
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    • pp.740-744
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    • 2014
  • In this study, the ${\beta}$-lactamase (VPA0477) gene was used as a new target for the PCR-based detection of Vibrio parahaemolyticus. Primers specific for the ${\beta}$-lactamase (VPA0477) gene of V. parahaemolyticus, were designed and incorporated into a PCR-based assay. The assay was able to specifically detect all of the 191 V. parahaemolyticus strains tested, but did not result in amplification of 39 other Vibrio spp. and non-Vibrio spp. strains tested. The detection limit of the assay was 10 CFU of V. parahaemolyticus RIMD2210633 from pure culture broth. The ${\beta}$-lactamase (VPA0477) gene-based assay developed in this study was sensitive and specific, and has great potential for the accurate detection and identification of V. parahaemolyticus in seawater or seafood samples.

Antimicrobial Effect of Lactic acid and Hydrogen Peroxide and Distribution of Vibrio parahaemolyticus from the Incheon Adjacent Sea (인천연안 Vibrio parahaemolyticus의 분포 및 유산과 과산화수소 처리에 의한 항균효과)

  • Jang, Jae-Seon;Cho, Woo-Kyoun;Lee, Hye-Jeong;Lee, Jea-Mann;Kim, Hye-Young;Kim, Yong-Hee
    • Journal of environmental and Sanitary engineering
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    • v.21 no.4
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    • pp.11-18
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    • 2006
  • This study was carried out to investigate the distribution of Vibrio parahaemolyticus in the Incheon adjacent sea, and antimicrobial effect on growth of Vibrio parahaemolyticus in lactic acid and hydrogen peroxide and combination of lactic acid and hydrogen peroxide. The detected strains were compared geographical, months and sample types. The distribution of Vibrio parahaemolyticus was high at Ganghwa county with 66.1%(336 samples), on 7-9 months with 72.4%(386 samples) and from tireland with 75.0%(90 samples), respectively. The minimun inhibitory concentration (MIC) of lactic acid in Vibrio parahaemolyticus were 1250 ppm at pH 6.5 and 7.0, 625 ppm at pH 6.0. respectively. The minimun inhibitory concentration (MIC) of hydrogen peroxide in Vibrio parahaemolyticus were 25 ppm at pH 6.5 and 7.0, 12.5 ppm at pH 6.0, respectively. MICs of combined treatment of lactic acid and hydrogen peroxide in Vibrio parahaemolyticus were 625 ppm of lactic acid with 12.5 ppm of hydrogen peroxide. The correlations between MICs of lactic acid and hydrogen peroxide in Vibrio parahaemolyticus were obtained through the coefficient of determination($R^2$). $R^2$ value were 1.0000. The antimicrobial effect of lactic acid and hydrogen peroxide in Vibrio parahaemolyticus could be confirmed from the result of this experiment.

The Biological Characteristics and Drug Resistance of Vibrio Species (Vibrio균속의 생물학적 성상 및 약제내성에 관하여)

  • Park, Chul-Hee;Lee, Yun-Tai
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.4
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    • pp.413-425
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    • 1987
  • In the present experiments, isolated Vibrio species from marine and clinical specimens from July, 1985 to October, 1986, had the results as follows: 1. The 55 strains of Vibrio were isolated and identified; Vibrio parahaemolyticus was 35 strains, Vibrio vulnificus was 10 strains, Vibrio alginolyticus was 10 strains. 2. In the K-serotyping of Vibrio parahaemolyticus, fourteen serotypes identified but three were not strains typable by the availble K-antisera. 3. In the Kanagawa phenomenon experiment of Vibrio parahaemolyticus, it proved positive reaction, 14 of 15 strains(93%) isolated from the patient and 13 of 20 strains(65%) isolated from the nature. 4. In twelve antibiotic resistance experiments, Vibrio parahaemolyticus and Vibrio alginolyticus showed 100% resistance on ampicilline, but Vibrio vulnificus showed 100% sensitivity. But all of them proved 100% sensitivity on chloramphenicol, tetracycline, nalidixic acid. 5. In the antibiotic resistance patterns, Vibrio parahaemolyticus proved that 15 strains(43%) resisted on 4 antibiotics and 5 strains(14%) resisted on 7 antibiotisc and. Vibrio vulnificus proved that 1 strain(10%) resisted on 2 antibiotics and 6 strains(60%) without resistance, Vibrio alginolyticus proved that 7 strains(70%) resisted on 3 antibiotics and 2 strains(20%) resisted on 8 antibiotics.

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Isolation and Identification of Vibrio vulnificus and Vibrio parahaemolyticus from Coast of Pusan and Daechon (부산과 대천 해안에서 Vibrio vulnificus와 Vibrio parahaemolyticus의 분리 및 동정)

  • Ju, Jin-Woo;Park, Min-Jung;Heo, Moon-Soo;Jung, Cho-Rok
    • The Journal of the Korean Society for Microbiology
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    • v.35 no.4
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    • pp.309-316
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    • 2000
  • This study was focused on the isolation of pathogenic Vibrio species, V. vulnificus and V. parahaemolyticus from marine environment from May to July of 1999. Isolation sites were coast near by Pusan and Daechon. The results obtained were as follows: 1. Seventy strains of V. parahaemolyticus and 19 strains of V. vulnificus were isolated from a total of 120 specimens. 2. Nineteen strains of V. vulnificus did not fermented arabinose and salicin but fermented lactose and cellobiose. All of V. parahaemolyticus isolates did not fermented lactose and cellobiose. 47 strains of V. parahaemolyticus fermented arabinose but 53 strains did not fermented salicin. 3. V. vulnificus and V. parahaemolyticus isolates showed three different API index numbers with 5046105 and 4346107 dominant. 4. V. vulnificus did not grow on 0% and 8% NaCl containing medium. V. parahaemolyticus grew on 8% NaCl containing medium. 5. V. vulnificus isolates and V. parahaemolyticus revealed different outer membrane protein profiles on SDS-PAGE.

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Studies on Vibrio Parahaemolyticus Food Poisoning (장염 Vibrio 식중독의 세균학적 연구)

  • 김자운
    • Journal of Environmental Health Sciences
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    • v.8 no.2
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    • pp.53-55
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    • 1982
  • The author was carried out bacteriological identification, and in order to evaluate the sensitivity of the different chemotherapeutic agents including chloramphenicol to Vibrio parahaemolyticus isolated from the stool of the patient's diarrhea. The results obtained were as follows: 1) Biochemical properties of Vibrio parahaemolyticus strains isolated from patients with diarrheal food poisoning was showed Table 1. 2) The sensitivity pattern of the isolated strains of Vibrio parahaemolyticus were sensitive to chloramphenicol, sulfonamide, kanamycin and colistin. But tetracycline, penicillin and leucomycin were resistant.

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Antimicrobial Effect of Grapefruit Seed Extract on Vibrio parahaemolyticus Isolated from the Southern Adjacent Sea of Korea (남해안연안에 분포되어 있는 Vibrio Parahaemolyticus의 성상 및 Grapefruit Seed Extract 처리에 의한 향균효과)

  • 조성환;강동훈;전상수;정덕화
    • Journal of Food Hygiene and Safety
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    • v.9 no.3
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    • pp.141-149
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    • 1994
  • To investigate the distribution of Vibrio parahaemopyticus in the southern 4 coastal areas, Vibrio parahaemolyticus was isolated from seawater, shellfish and sediment from May to October in 1991, and antimicrobial effect of grapefruit seed extract (GFSE) on the growth of isolated strains were examined. In the 120 sample from 4 coastal areas, 16 strains of Vibrio parahaemolyticus were isolated and identified. The distribution serotype of isolated strains was 10 types of monovalent k-antiserum. Especially k-5 and k-28 were highly distribyted with 3 and 4 strains. 31.3% of isolated strains showed positive on Kanagawa phenomenon test. All isolated Vibrio parahaemolyticus were sensitive to chloramphenicol and gentamycin, 5 and 6 strains were resistant to streptomycin and colistin, respectively. Isolated strains were compared with geographical, month and sample. The distribution of 16 isolated Vibrio parahamolyticus was high at Hadong with 50%(8 strains), on July with 43.8%(7 strains) and from seawater with 37.5%(6 strains) respectively. Minimal inhibitory level of GFSE to Vibrio parahaemolyticus was 50 ppm. With 100 ppm treatment of GFSE, the destroy of cell membrane function, outflow of cell ingredients and ghost morphology of cell were investigated.

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Distribution of Vibrio parahaemolyticus and Vibrio vulnificus in Seawater and Shellfish at Gomso Bay (곰소만의 해수 및 패류에 있어서 장염비브리오균(Vibrio parahaemolyticus)과 비브리오패혈증균(Vibrio vulnificus)의 분포)

  • Lee, Dong-Soo;Shin, Hye-Young;Park, Kwon-Sam;Shin, Il-Shik
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.2
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    • pp.114-120
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    • 2019
  • In the case of being contaminated with pathogenic Vibrio, shellfish pose a serious threat for public health. This study was conducted to investigate the presence of Vibrio parahaemolyticus and V. vulnificus in the seawater, bottom deposit and shellfish samples collected from the Gomso Bay, west coast of Korea. V. parahaemolyticus (30-80 CFU/g) was detected from seawater Jul. to Oct. and highest at Sep. V. parahaemolyticus was detected less than 10 CFU/g from seawater at Nov. with $14.6^{\circ}C$ of seawater temperature. V. parahaemolyticus (180-1,850 CFU/g) was detected more than 100 CFU/g from 3 kinds of shellfish, Short-necked clam Ruditapes philippinarum, Corb shell Cyclina sinensis, and Surf clam Mactra veneriformis, Jul. to Oct. and was highest in Surf clam at Oct. V. parahaemolyticus was detected less than 20 CFU/g from all tested shellfish at Nov. V. vulnificus (1.8-2.7 MPN/100 mL) was detected from seawater Jul. to Oct. and was not detected at Nov. V. vulnificus (18-236.7 MPN/100 g) was also detected from 3 kinds of shellfish Jul. to Oct. and was highest in Corb shell at Jul. V. vulnificus was not detected from all tested shellfish at Nov.

A Profile of Naturally Occurring Plasmids from Selected Strains of Vibrios

  • Kim, Young-Hee
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.1 no.2
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    • pp.93-97
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    • 1997
  • The naturally occurring plasmids of Vibrio species have been isolated in part to investigate their genetic traits. Among six different Vibrio species tested, Vibrio anguillarum, Vibrio fluvialis, Vibrio vulnficus, Vibrio mimicus and Vibrio furnissi did not show any presence of plasmid. One environmental isolate of Vibrio parahaemolyticus harboring plasmid was observed. The isolated plasmid was 8.7 kb by analysis with restriction endonuclease digestion. No common feature was shown relationships between the presence of plasmid and resistance against commonly used antibiotic compounds from the tested Vibrios.

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Detection and Identification of Vibrio Species Using Whole-Cell Protein Pattern Analysis

  • Lee, Chae-Yoon;Hong, Yeun;Ryu, Jio;Kim, Young-Rok;Oh, Sang-Suk;Lee, Soon-Ho;Hwang, In-Gyun;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • v.22 no.8
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    • pp.1107-1112
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    • 2012
  • Outbreaks of foodborne diseases associated with Vibrio species such as V. parahaemolyticus, V. vulnificus, and V. cholerae frequently occur in countries having a dietary habit of raw seafood consumption. For rapid identification of different Vibrio species involved in foodborne diseases, whole-cell protein pattern analysis for 13 type strains of 12 Vibrio species was performed using SDS-PAGE analysis. Pathogenic Vibrio species such as V. parahaemolyticus, V. vulnificus, V. cholerae, V. alginolyticus, V. fluvialis, and V. mimicus were included in the 12 Vibrio species used in this study. Each of the 12 Vibrio species showed clearly specific band patterns of its own. Two different strains of V. parahaemolyticus showed two different SDS-PAGE whole-cell protein patterns, giving the possibility of categorizing isolated strains in the same V. parahaemolyticus species into two subgroups. The 36 Vibrio isolates collected from sushi restaurants in Busan were all identified as V. parahaemolyticus by comparing their protein patterns with those of Vibrio type strains. The identified isolates were categorized into two different subgroups of V. parahaemolyticus. The whole-cell protein pattern analysis by SDS-PAGE can be used as a specific, rapid, and simple identification method for Vibrio spp. involved in foodborne diseases at the subspecies level.