• Title/Summary/Keyword: Vimentin

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An Immuno-Electronmicroscopic Study on the Synoviocytes in the Knee Joint of the Human (인체 무릎관절 윤활세포에 관한 면역전자현미경적 연구)

  • Hwang, Douk-Ho;Chang, Ka-Young;Lee, Wang-Jae;Park, Kyung-Han;Lee, Jong-Bum
    • Applied Microscopy
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    • v.26 no.1
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    • pp.11-16
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    • 1996
  • This study was designed to observe the ultrastructure of synoviocytes which are concerned with phagocytic function in the knee joint of the human. The synovia were dissected and were fixed for two hours in 0.2% glutaraldehyde and 4% paraformaldehyde solution and processed and finally infused in 2.3 M sucrose and 20% PVP solution. The tissues were cut with the cryoultramicrotome and labelled with primary antibodies (anti-tubulin, anti-vimentin) and secondary antibody-6 nm colloidal gold particles. The tissues were observed under transmission electronmicroscope. The results were followings. 1. In phagocytic synovial cells, the distributions of tubulin were cytoplasm, especially around vacuoles. 2. In phagocytic synovial cells, the distributions of vimentin were cytoplasm. 3. Both tubulin and vimentin were not located inside of vacuoles. On the basis of above findings, it is obvious that the phagocytic functions are concerned with tubulin, and the phagocytic synovial cells contain vimentin.

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Overexpressions of Vimentin and Integrins in Human Metastatic Spine Tumors

  • Park, Sung Bae;Ryu, Young-Joon;Chung, Young Seob;Kim, Chi Heon;Chung, Chun Kee
    • Journal of Korean Neurosurgical Society
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    • v.57 no.5
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    • pp.329-334
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    • 2015
  • Objective : To comparatively investigate the expression of several integrins in specimens of human bone metastases and degenerative bone tissue. Methods : Degenerative cancellous tissue was obtained from a sample of human degenerative spine. Thirteen human specimens were obtained from metastatic spine tumors, whose primary cancer was colon cancer (n=3), hepatocellular cancer (n=3), lung cancer (n=4), and breast cancer (n=3). The expression of vimentin and integrins ${\alpha}v$, ${\beta}1$, and ${\beta}3$ was assessed in metastatic and degenerative specimens by immunohistochemistry and real-time reverse transcription-polymerase chain reaction (qRT-PCR). Results : Immunohistochemical staining showed that vimentin and integrin ${\alpha}v$ was broadly expressed in all tissues examined. By contrast, integrin ${\beta}1$ was weakly expressed only in 38.4% (5/13) of tissues. Integrin ${\beta}3$ was consistently negative in all cases examined. qRT-PCR analysis showed that vimentin gene expression was higher in all metastatic specimens, as compared to degenerative bone. The gene expression of integrin ${\alpha}v$ in breast specimen was significantly higher than others (p=0.045). The gene expression of integrin ${\beta}1$ was also higher in all metastatic specimens than in degenerative bone tissue. The gene expression of integrin ${\beta}3$ was variable. Conclusion : Spinal metastatic tumors have mesenchymal characteristics such as increased expression of vimentin. The increased expression of integrin ${\alpha}v$ and ${\beta}1$ in spine metastatic tumors suggests that adhesive molecules such as integrin may have implications for the prevention of spine metastasis.

Carcinosarcoma of the Esophagus with Cartilagenous Production -A Case Report - (연골 분화를 보인 식도 암육종 -1례 보고-)

  • 양수호;이철범;한동수;안명주;백홍규;함시영;정원상;강정호;지행옥
    • Journal of Chest Surgery
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    • v.31 no.4
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    • pp.422-426
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    • 1998
  • Progressive dysphagia in a 53 year old man was caused by a giant polypoid tumor in the lower intrathoracic esophagus. Radical transthoracic esophagectomy and esophagogastrostomy were carried out. Microscopic examination of the tumor revealed a true carcinosarcoma, composed of a mixture of basaloid squamous cell carcinoma and chondrosarcoma with multiple cartilagenous productions. Carcinoma metastases were found in the subcarinal and perigastric lymph nodes. Immunohistochemically, squamous area displayed strong positive to cytokeratin, and basaloid area showed positive immunoreaction to high molecular weight cytokeratin (34${\beta}$E12). Spindle cell sarcoma reacted to vimentin and smooth muscle actin. Chondrosarcomatous area reacted to vimentin and S-100 protein. He received postoperative chemotherpy and radiotherapy. He has been free of disease for 11 months.

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Low Expression of the FoxO4 Gene may Contribute to the Phenomenon of EMT in Non-small Cell Lung Cancer

  • Xu, Ming-Ming;Mao, Guo-Xin;Liu, Jian;Li, Jian-Chao;Huang, Hua;Liu, Yi-Fei;Liu, Jun-Hua
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.9
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    • pp.4013-4018
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    • 2014
  • Because of its importance in tumor invasion and metastasis, the epithelial-mesenchymal transition (EMT) has become a research focus in the field of cancer. Recently, evidence has been presented that FoxO4 might be involved in EMT. Our study aimed to detect the expression of FoxO4, E-cadherin and vimentin in non-small cell lung cancers (NSCLCs). We also investigated clinical features and their correlations with the markers. In our study, FoxO4, E-cadherin and vimentin were assessed by immunohistochemistry in a tissue microarray (TMA) containing 150 cases of NSCLC. In addition, the expression level of FoxO4 protein was determined by Western blotting. The percentages of FoxO4, E-cadherin and vimentin positive expression in NSCLCs were 42.7%, 38.7% and 55.3%, respectively. Immunoreactivity of FoxO4 was low in NSCLC when compared with paired normal lung tissues. There were significant correlations between FoxO4 and TNM stage (P<0.001), histological differentiation (P=0.004) and lymph node metastasis (P<0.001), but no significant links with age (P=0.323), gender (P=0.410), tumor size (P=0.084), smoking status (P=0.721) and histological type (P=0.281). Our study showed that low expression of FoxO4 correlated with decreased expression of E-cadherin and elevated expression of vimentin. Cox regression analysis indicated FoxO4 to be an independent prognostic factor in NSCLC (P=0.046). These data suggested that FoxO4 might inhibit the process of EMT in NSCLC, and might therefore be a target for therapy.

Distinction Between Reactive Mesothelial and Carcinoma Cells in Serous Effusions by Mucin- and Immune-cytochemical Panel (점액 및 면역 세포화학적 Panel 염색에 의한 장액성 삼출액내 반응성 중피세포와 암종세포의 감별)

  • Kim, Byung-Heon
    • The Korean Journal of Cytopathology
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    • v.9 no.1
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    • pp.1-14
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    • 1998
  • The cytologic distinction of carcinoma cells from reactive mesothelial cells can be difficult, especially in specimens containing abundant reactive mesotheilal cells and inflammatory cells with scant carcinoma cells. This study evaluates the usefulness of mucin and immunocytochemistry for discrimination between reactive mesotheilal cells and carcinoma cells, and sensitivity and specificity of these stains for the detection of metastatic carcinoma in serous effusions. Immunocytochemical panel including mucin cytochemistry with the periodic acid-Schiff(PAS) reaction after or without diastase digestion was undertaken on 127 serous effusion specimens with histologically confirmed diagnoses. The specimens including cell smears and cell blocks were stained with PAS and antibodies to carcinoembryonic antigen(CEA), epithelial membrane antigen(EMA), cytokeratln(CK), and vimentin. The sensitivities of these stains for metastatic carcinoma(127 cases) were 49%(46/94) in PAS, 48%(60/124) in CEA, 89%(97/109) in EMA, 88%(93/106) in CK, and 25%(20/81) in vimentin. The sensitivities of stains for reactive mesothelial cells(36 cases) were 19%(7/36) in EMA, 78%(28/36) in CK, and 75%(27/36) in vimentin. The PAS and CEA stains were not reacted with all cases of benign reactive serous effusions containing abundant reactive mesothelial cells. The specificities of stains for metastatic carcinoma(127 cases) were 100% in PAS, 100% in CEA, 81% in EMA, 22% in CK, and 25% in vimentin. The optimal combination of stains for use in a panel was PAS and CEA. Combined results from these two stains yielded an advanced sensitivity of 8% in PAS and 4% in CEA for metastatic carcinoma. EMA was also cosiderably useful for identification of carcinoma cells. CK and vimentin were not suitable for distinguishing between reactive mesothelial cells and carcinoma cells.

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Effect of Glial-neuronal Cell Co-culture on GFAP Expression of Astrocytes (신경세포가 별아교세포의 아교섬유성 산단백질 표현에 미치는 영향)

  • Bae Hyung-Mi;Park Jung-Sun;Yeon Dong-Soo
    • The Korean Journal of Physiology and Pharmacology
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    • v.1 no.3
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    • pp.285-296
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    • 1997
  • Injury to brain transforms resting astrocytes to their reactive form, the hallmark of which is an increase in glial fibrillary acidic protein (GFAP), the major intermediate filament protein of their cell type. The overall glial response after brain injury is referred to as reactive gliosis. Glial-neuronal interaction is important for neuronal migration, neurite outgrowth and axonal guidance during ontogenic development. Although much attention has been given to glial regulation of neuronal development and regeneration, evidences also suggest a neuronal influence on glial cell differentiation, maturation and function. The aim of the present study was to analyze the effects of glial-hippocampal neuronal co-culture on GFAP expression in the co-cultured astrocytes. The following antibodies were used for double immunostaining chemistry; mouse monoclonal antibodies for confirm neuronal cells, rabbit anti GFAP antibodies for confirm astrocytes. Primary cultured astrocytes showed the typical flat polygonal morphology in culture and expressed strong GFAP and vimentin. Co-cultured hippocampal neurons on astrocytes had phase bright cell body and well branched neurites. About half of co-cultured astrocytes expressed negative or weak GFAP and vimentin. After 2 hour glutamate (0.5 mM) exposure of glial-neuronal co-culture, neuronal cells lost their neurites and most of astrocytes expressed strong CFAE and vimentin. In Western blot analysis, total GFAP and vimentin contents in co-cultured astrocytes were lower than those of primary cultured astrocytes. After glutamate exposure of glial-neuronal co-culture, GFAP and vimentin contents in astrocytes were increased to the level of primary cultured astrocytes. These results suggest that neuronal cell decrease GFAP expression in co-cultured astrocytes and hippocampal neuronal-glial co-culture can be used as a reactive gliosis model in vitro for studying GFAP expression of astrocytes.

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Pathologic observations on the canine mammary gland tumors and immunohistochemical study on the origin of chondroid tissue in mammary gland mixed tumors (개 유선종양의 병리학적 관찰 및 유선 혼합종양의 연골기원에 관한 면역조직화학적 연구)

  • Kim, Yong-baek;Seo, Il-bok;Kim, Jae-hoon;Bak, Eun-jung;Kim, Dae-yong;Han, Jeong-hee
    • Korean Journal of Veterinary Research
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    • v.37 no.4
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    • pp.843-854
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    • 1997
  • Sixteen mammary gland tumors were collected from Seoul National University and Kangwon National University. The average age of the bitches with mammary gland tumor was 10 years. Total 17(60.7%) out of 28 tumor masses observed in 4th and 5th glands. Classification of these tumors according to Hampe and Misdorp were simple adenoma, complex adenoma, benign mixed tumor, papillary adenocarcinoma, solid adenocarcinoma and malignant mixed tumor. Immunohistochemical reaction of the intermediate filaments against normal canine mammary gland showed as followed; anti-cytokeratin 18 was strong and anti-cytokeratin 14 was moderate to the luminal epithelium. Anti-cytokeratin 14 and anti-pancytokeratin to the myoepithelium were showed strong, but anti-vimentin was weak in reactivity. Anti-vimentin to the interstitial cells was represented strong reactivity. The origin of cartilage in mixed tumor of canine mammary gland was studied immunohistochemically with antibodies against intermediate filament. In mammary gland mixed tumors, cartilage tumor tissues were surrounded with the irregularly demarcated three zones composed of adjacent star shaped cells in myxoid areas, proliferative spindle shaped cells and basal located proliferated cells. From basal proliferated cells to star shaped cells, the immunohistochemical reactivity of myoepithelium specific anti-pancytokeratin was decreased gradually and the reactivity of interstitial cell specific anti-vimentin was increased gradually. Based on these immunohistochemical staining patterns, we suggested that the origin of cartilagenous components in canine mammary gland mixed tumor is most likely to the proliferation and metaplsia of myoepithelium.

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Histological Features of the Testis in the Long-beaked Common Dolphin from Korean Waters

  • Kim, Juhwan;Jang, Sungwoong;Jeon, Yubyeol;An, Du Hae;Kim, Doo Nam;Hyun, Sang-Hwan;Moon, Changjong
    • Journal of Embryo Transfer
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    • v.28 no.1
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    • pp.73-78
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    • 2013
  • The present study examined the histological characteristics of adult testis in the long-beaked common dolphin (Delphinus capensis) from Korean waters and the localization of DEAD-box polypeptide 4 (DDX4; a germ cell marker) and vimentin (a Sertoli cell marker) expression in the dolphin testis compared with that in terrestrial mammals, including dogs and rats. The seminiferous tubules of dolphin testis have very small or completely closed lumens, and spermatogenic cells and Sertoli cells within the tubules cannot be differentiated. Immunohistochemical analysis showed that, in the dolphin testis, DDX4- and vimentin-positive cells were scattered extensively within the tubule, whereas in the dog and rat testis, DDX4 immunoreactivity was localized in spermatogenic cells of the adluminal compartment, and vimentin immunoreactivity was localized in Sertoli cells of the basal compartment in the seminiferous epithelium. These results suggest that the histological characteristics of the seminiferous tubules in the dolphin testis differ from those of terrestrial species.

Effect of Exercise on Muscle Recovery of Sciatic Nerve Injured Rats (궁둥신경 손상을 유발한 흰쥐에서 운동이 근육재생에 미치는 영향)

  • Ro, Hyo-Lyun
    • Proceedings of the KAIS Fall Conference
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    • pp.855-858
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    • 2010
  • 이 연구의 목적은 흰쥐의 궁둥신경의 손상을 준 뒤 달리기운동을 시켜 운동이 근육재생에 효과가 있는지를 밝힐 목적으로 시행하였다. 수컷 Sprague-Dawley계 흰쥐의 궁둥신경을 압궤손상을 준후, 하루 60분 씩 주 5회 운동을 시켰다. 궁둥신경 압궤손상 후 12일(5일간 운동), 19일(10일간 운동), 26일(15일간 운동), 33일(20일간 운동 및 61일(40일간 운동)에 흰쥐를 희생시켜 장딴지근육을 절취하여 냉동절편을 만들었으며 중간세사인 desmin과 vimentin에 대한 면역조직화학적 염색방법을 시행하였고 NADH-TR을 이용한 효소조직화학적 방법으로 뼈대근육의 변성 등을 관찰하였다. 중간세사단백 중 desmin은 근육섬유의 변성 및 재생과정 모두에서 발현되며 vimentin은 재생과정에서만 발현되었다. 대조군보다 실험군에서 desmin과 vimentin의 면역 반응성이 높았고, 실험군의 근육섬유들은 손상 후 61일째에 횡단면에서 각을 갖고 있어 정상 근육섬유임을 입증해 주었으나 대조군의 근육섬유는 정상으로 회복되지 않은 둥근 근육섬유가 관찰되었다. 19일째의 NADH-TR반응에서 대조군에서는 근육 섬유형 군집(fiber type grouping)으로 인해 diformazan 과립이 뭉쳐져 있음을 볼 수 있으나 실험군에서는 점점 diformazan 과립이 고르게 분포됨을 볼 수 있었다. 재신경지배가 일어 날 때 나타나는 표적근육섬유(target fiber)는 NADH-TR반응에서 26일 실험군의 일부 근육섬유에서 처음으로 관찰되었다. 61일째 NADH-TR반응에서 대조군은 아직도 diformazan 과립이 근육섬유성 군집을 보였으나 실험군에서는 정상군과 다름없는 염색성이 관찰되었다. 이상의 결과로 흰쥐 궁둥신경 압궤손상 후 트레드밀 달리기 운동이 흰쥐 다리 뼈대근육의 중간세사발현에 효과가 있다고 생각된다.

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