• Title, Summary, Keyword: Y. enterocolitica

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Growth Characteristics and Plasmid Profiles of Yersinia enterocolitica lsolated from Springs Water (약수터수로부터 분리한 Yersinia enterocolitica의 성장특성 및 Plasmid 유형)

  • 차인호;김미희;이상준
    • Journal of Life Science
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    • v.7 no.3
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    • pp.212-218
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    • 1997
  • The studies were conducted to explore the dffects of growth or survival against various factors and plasmid profiles of 49 Y. enterocolitica isolated from springs water. In the presence of calcium hypochlorite, y. enterocolitica was entirely extinguished by exposure for 33 hours at 0.8 ppm concentration, and was grown up to 7% NaCl, but not at 95 NaCI. Y. enterocolitica was presented optimal growth at pH 7.0 anad 9.0, and not allowth the growth at pH3.0, 5.0 and 11.0. The optimal temperature for growth of Y. enterocolitica was 25$\circ$C and 35$\circ$C, and allowed the growth at refrigerant temperature, 5$\circ$C. Y. enterocolitica was remarkably decreased by exposure for 30 seconds under UV light, and entirely extinguished by exposure for 90 seconds. Therefore, UV light was effective for sterilization of Y. enterocolitica. Fourty-nine strains of Y. enterocolitica were harbor plasmid DNA of approximately 46 Kb molecular weight.

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Effects of Glycine Betaine and Related Osmolytes on Growth of Osmotically Stressed Yersinia enterocolitica (삼투압 스트레스를 받은 Yersinia enterocolitica의 성장에 미치는 glycine betaine을 비롯한 osmolyte의 영향)

  • Park, Shin
    • Applied Biological Chemistry
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    • v.38 no.3
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    • pp.218-223
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    • 1995
  • Osmolytes accumulated in the osmotically stressed Yersinia enterocolitica ATCC 9610 were investigated using natural abundance $^{13}C$ NMR spectroscopy. Glycine betaine, one of the more common and most effective osmolytes found in nature, was the dominant osmolyte in osmotically stressed Y. enterocolitica cells. Glycine betaine concentration was 41.8 times higher (801.9 nmol/mg protein) in stressed cells than in unstressed cells (19.2 nmol/mg protein). Proline was the minor osmolyte, and its concentration was 284.8 nmol/mg protein. The effects of glycine betaine and related osmolytes on growth rate of osmotically stressed Y. enterocolitica were investigated to identify their ability as osmolytes for Y. enterocolitica. When glycine betaine and proline were added in MMA medium containing 2.5% NaCl, the growth rate with glycine betaine (1 mM) was 3.6 times higher than in control (no addition of osmolyte), and that with proline was 1.3 times higher. Dimethylglycine (5 mM) also increased the growth rate 3.1 folds. On the other hand, monomethylElycine had no effect on growth of osmotically stressed and unstressed Y. enterocolitica. When carnitine was added in MMA medium containing 2.5% NaCl, carnitine (5 mM) increased the growth rate 2.4 folds, but choline had no effect on growth of osmotically stressed Y. enterocolitica. The above results indicate that glycine betaine is the dominant osmolyte in osmotically stressed Y. enterocolitica, and proline, dimethylglycine and carnitine also act as minor osmolytes.

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Prevalence of antibody against 38 kDa outer membrane protein of Yersinia enterocolitica in swine (국내 사육돼지에서의 Yersinia enterocolitica 38 kDa outer membrane protein에 대한 항체가 분포)

  • Shin, Seong-jae;Park, Joo-youn;Choi, In-soo;Shin, Na-ri;Yoo, Han-sang
    • Korean Journal of Veterinary Research
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    • v.41 no.1
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    • pp.73-78
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    • 2001
  • Yersinia enterocolitica is an inhabitant in the lower intestinal tract of many domestic and wild animals as well as in the nature. Of the several forms of diseases caused by Y. enterocolitica, an acute enteritis, especially in young children, is the most common form. Infection of the bacteria usually occurs through fecal-oral route by contaminated foods or water, especially mountainspring water. Of the domestic animals, swine has been known as one of the most important carrier in the human infection. Based on the knowledge, prevalence of antibody against Y enterocolitica was investigated with swine sera collected from Korea for the survey of Y enterocolitica infection in swine. As the first step of this survey, we analyzed outer membrane protein (OMP) profiles of the representative strains of Y enterocolitica isolated from the feces of piglets and mountainspring water in Korea. Thirty-eight kDa OMP was identified as the common OMP regardless of origin, serotype, or biotype of Y enterocolitica isolates. Presence of antibody specific for 38 kDa OMP of Y enterocolitica in 1,076 swine sera collected from November 1999 to October 2000 was analysed with ELISA. Antibody titer in sows was significantly higher than that in piglets, growing pigs and finishing pigs (p<0.05). Also, there was seasonal difference in the prevalence of antibody against Y enterocolitica. These results would provide the basic knowledge for controlling the Y enterocolitica infection in human as well as swine.

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Biotype, serotype and antibiotics susceptibility of Yersinia enterocolitica isolated from zoo animals (동물원(動物園) 야생동물(野生動物)에서 분리(分離)한 Yersinia enterocolitica 의 생물형(生物型), 혈청형(血淸型) 및 항생제(抗生劑) 감수성(感受性))

  • Park, Seog-gee;Youn, En-sun;Kim, En-jung
    • Korean Journal of Veterinary Research
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    • v.34 no.1
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    • pp.85-91
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    • 1994
  • A study on the isolation of Yersiniae from the feces of wild animals(mammals 376, birds 19 and reptiles 13) in zoo and the biotype and serotype and susceptibility of 12 antibiotics was carried. Out of 408 animals, Yersiniae were isolated from 28 animals(6.9%). Of 28 isolates, 27 isolates(96.4%) were Y. enterocolitica and 1(3.6%) was Y. kristensenii. According to the species, 25(6.6%) of Y. enterocolitica and 1(0.3%) of Y. kristensenii were isolated from 376 mammals, 2(15.4%) of Y. enterocolitica from 13 reptiles but not isolated from 19 birds. According to the eating pattern, 8(5.2%) of Y. enterocolitica were isolated from 155 carnivora, 13(10%) of Y. enterocolitica from 123 herbivora, and 6(4.9%) of Y. enterocolitica and 1(0.8%) of Y. enterocolitica from 123 omnivora. Out of 27 isolates of Y. enterocolitica, all were biotype 1. And predominant serotype was 0:21(40.7%), and 0:5(37.0%), 0:6(11.1%), 0:1(3.7%), 0:9(3.7%) and untypable(3.7%). Yersiniae isolated from zoo animals were resistant to cephalothin(100%), ampicillin(96.4%), carbenicillin(96.4%) and tetracycline(14.3%) and streptomycin(3.6%) and susceptible to chloramphenicol(100%), colistin(100%), gentamicin(100%), kanamycin(100%), nalidixic acid(100%), polymyxin B(100%) and tobramycin(100%). The predominant multiple resistance pattern was Am-Cf-Cb(82.1%), and Am-Cf-Cb-Te(10.7%) and Am-Cf-Cb-Te-Sm(3.7%).

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Characteristics of Yersinia enterocolitica Isolated from Foods (식품에서 분리한 Yersinia enterocolitica의 특성조사)

  • Lim, Soon-Young;Lee, Dong-Ha;Park, Sun-Hee;Park, Young-Sig;Yoon, Suk-Kwon;Kim, Chang-Min
    • Korean Journal of Food Science and Technology
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    • v.31 no.1
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    • pp.183-188
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    • 1999
  • The incidence of Yersinia enterocolitica in raw meat was determined over 10 month period. Y. enterocolitica was isolated from 8.5% of beef, 17.0% of pork and 25.6% of chicken. Overall prevalence of Y. enterocolitica in raw meat was 17.5%. Seasonal difference was observed in isolation rate in which pork and chicken samples collected in the second half of the year twice was more than that of the first half of the year. Serotypes of Y. enterocolitica isolates were O:5 (17.3%), O:8 (8.6%), O:3 (6.2%), O:1,2 (1.2%), and others. The antibiotics susceptibility tests showed Y. enterocolitica isolates were resistant to carbenicillin, ampicillin, erythromycin, penicillin and bacitracin. In contrast it showed sensitivity to polymyxin B, kanamycin, ciprofloxacin, gentamicin, trimethoprim/sulfamethoxazole, nalidixic acid, and tetracycline. PCR with specific primers derived from ail gene of Y. enterocolitica was applied to detect and confirm pathogenic Y. enterocolitica. About 10% of the isolated Y. enterocolitica proved to be pathogenic and most of them were found in pork. However, proper cooking and meat process can kill and remove all Y. enterocolitica in meat, because the organism is very sensitive to heat.

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Predicting the Contamination of Listeria Monocytogenes and Yersinia enterocolitica in Pork Production Using Monte Carlo Simulation (몬테카를로 시뮬레이션을 이용한 돈육 생산공정에서의 Listeria monocytogenes 및 Yersinia enterocolitica의 오염수준 예측)

  • Rho, Min-Jeong;Chung, Myung-Sub;Park, Ji-Yong
    • Korean Journal of Food Science and Technology
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    • v.35 no.5
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    • pp.928-936
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    • 2003
  • Monte Carlo simulation was used to predict the contamination levels of Listeria monocytogenes and Yersinia enterocolitica in final pork products. Mean values of the estimated log contaminated levels of L. monocytogenes on carcasses, cut meats, and cut meats after storage were -4.59, -4.46 and -4.45 $log_{10}CFU/cm^2$ respectively. The mean values of estimated log contaminated levels of Y. enterocolitica on carcasses, cut meats, and cut meats after storage were -3.44, -4.00 and -3.97 $log_{10}CFU/cm^2$, respectively. Sensitivity analysis showed that L. monocytogenes and Y. enterocolitica in pork was most sensitive to the prevalence of L. monocytogenes and Y. enterocolitica in the equipment used.

Detection of Pathogenic Yersinia enterocolitica Strains by a Rapid and Specific Multiplex PCR Assay

  • Kim Young-Sam;Kim Jong-Bae;Eom Yong-Bin
    • Biomedical Science Letters
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    • v.10 no.4
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    • pp.333-339
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    • 2004
  • A multiplex PCR assay targeting the yst and 16S rRNA genes of Yersinia enterocolitica was developed to specifically identify pathogenic Y. enterocolitica from pure culture. Simultaneous amplification of 145 and 416 bp fragments of the yst and 16S rRNA genes of Y. enterocolitica was obtained using the primer pairs in a single reaction. Validation of the assay was performed with the reference Yersinia strains and other members of the family Enterobacteriaceae. The defined primer pairs amplified the targeted sequence from only pathogenic Y. enterocolitica strains, whereas none of the other bacterial species yielded any amplified fragments. Within an assay time of 4 h, this assay offers a very specific, reliable, and inexpensive alternative to the conventional phenotypic assays used in clinical laboratories to identify pathogenic Y. enterocolitica.

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Comparison of Biotyping, Serotyping and Molecular Typing of Yersinia enterocolitica Isolated from Spring water in Seoul (서울시내 약수에서 분리한 Yersinia enterocolitica의 생물형, 혈청형 및 분자학적 형별비교)

  • 이영기;최성민;오수경;신재영
    • Journal of environmental and Sanitary engineering
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    • v.14 no.4
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    • pp.99-109
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    • 1999
  • Enteropathogenic Yersina enterocolitica is an important cause of human and animal disease. Phenotypic and genotypic characteristics currently used to identify Yersinia enterocolitica are not necessarily sufficient to differentiate pathogenic from non-pathogenic strains or to analyze the epidemiology of yersiniae at a molecular level. To improve the characterization of Yersinia enterocolitica, A total of 65 isolates of Yersinia enterocolitica were examined with bioserotyping, antibiotic susceptibilities, PFGE, PCR-ribotyping. Genomic DNA pattern generated by PFGE are highly specific for different strains of an organism and have significant value in epidemiologic investigations. The PFGE analysis of Not I-digested chromosomal DNA of Y. enterocolitica were performed with a CHEF Mapper(Bio-Rad, USA). Not I generated 19 restriction endonuclease digestion profiles(REDP). PCR-ribotyping, performed with primers complementry to conserved regions of 16S and 23S rRNA gene, generated 13 ribotypes. PCR-ribotyping can be considered a good technich for subtyping strains of Y.enterocolitica.

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Characteristics of Yersinia enterocolitica Isolated from Frozen Foods (냉동식품에서 분리한 Yersinia enterocolitica의 특성)

  • Lim, Soon-Young;Yoon, Suk-Kwon
    • Korean Journal of Food Science and Technology
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    • v.32 no.6
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    • pp.1336-1340
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    • 2000
  • Overall prevalence of Y. enterocolitica in frozen foods was 5.6% (35 cases of 624 samples). Seasonal variation of contamination was observed. Isolation rate of Y. enterocolitica from samples collected in the second half of the year was six times higher than those of the first half of the year. Serotype of the isolated Y. enterocolitica was mainly serotype O:5 (9 cases). However, 25 cases of 35 isolates could not be serotyped with antiserum used in this study. The biotype test showed that all isolates were non-pathogenic type 1A. The polymerase chain reaction test with ail gene specific primers also confirmed that pathogenic strains were not found in frozen food isolates.

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Detection of Pathogenic Yersinia Enterocolitica in Drinking Water and Vegetables by Mutiplex-PCR (Multiplex-PCR에 의한 먹는샘물 및 야채류로부터의 병원성 Yersinia enterocolitica의 신속검출)

  • 이택수;박부길;오덕환
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.1
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    • pp.35-41
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    • 2003
  • The study was conducted to develope a rapid method for the detection of Yersinia enterocolitica in spring water and vegetables via multiplex polymerase chain reaction (PCR) technique using ail, yst, uirF and subgenus-specific Y16S primers. Specificity and sensitivity of multiplex PCR and application of best primers for the detection of Y. enterocolitica from spring water and vegetables were investigeted. Y. enterocolitica ATCC 27729 strains gave 356 bP and 200 bp (Y16S) and 134 bp (yst) bands. but Y. enterocolitica ATCC 9610 and ATCC 23715 strains gave 200 bp and 134 bp bands.In the meanwhile, non-pathogenic Yersinia species, such as Y. frederikseni, Y. inter-media, Y. kristenseni and Y. pseudotuberculosis gave only single 200 bp band, and other bacteria including Escherichia coli O157:H7 ATCC 25392, Shigella dysenteri. Staphylococcu aureus ATCC 25923 and Listeria mo-nocytogenes ATCC 19111 did not show any bands. Among primers, yst and Y16S primer showed the best sensitivity. Seven CFU/mL Y. enterocolitica cells could be detected with yst and Y16S primers and the sensitivity was significantly improved by the further 2nd PCR after 38 cycles of first PCR amplication. Spring water, cabbage and mushroom were inoculated with Y. enterocolitica to determine the sensitivity of multiplex-PCR for the rapid detection of Y. enterocolitica. Multiplex-PCR assay could detect 7 or 70 cells in spring water and vegetables using whole cell lysate with repeating PCR amplication.