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Inhibitory Effect of Garlic on the Growth of Aspergillus parasiticus (한국산 마늘에 의한 Aspergillus parasiticus의 성장 억제 효과)

  • Park, Jeong-Yeong;Kim, Jong-Gyu
    • Journal of Environmental Health Sciences
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    • v.35 no.2
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    • pp.95-99
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    • 2009
  • This study was performed to investigate the possible effect of garlic produced in Korea on the inhibition/reduction of growth of A. parasiticus, a toxigenic strain. The effect was studied using different concentrations of freeze-dried garlic in potato-dextrose agar (PDA) and/or in yeast-extract sucrose (YES) broth at $25^{\circ}C$ for 15 days. While inhibition of the fungal growth due to increasing the concentration of garlic was observed, the more remarkable effect was observed on the ninth day. Reduction of fungal diameter as a result of addition of garlic on PDA was observed to range between 3.4% to 20.1 % while reduction of mycelial weight in YES broth ranged from 9.9% to 30.5%. The 0.5% and 1.0% concentrations of garlic significantly reduced fungal diameter in PDA on the 9th day, while 0.1 %, 0.5%, and 1.0% concentrations of garlic significantly reduced the mycelial weight in YES broth (p<0.05). Dose-response relationships were observed between the concentration of garlic and inhibition of growth both in solid culture and in liquid culture. This study indicates that garlic could be an effective inhibitor at a human consumption level of the growth of A. parasiticus. More research is needed to study the inhibitory effects of the main active component of garlic.

Effect of Sunlight on the Reduction of Mycelia and Aflatoxins (태양광선에 의한 Aflatoxin의 감소 효과)

  • 변영희;김종규
    • Journal of Food Hygiene and Safety
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    • v.14 no.4
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    • pp.428-432
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    • 1999
  • This study was performed to investigate the possible effect of sunlight on the reduction or degradation of mycelia and aflatoxins. The mycelia and aflatoxins were produced by Aspergillus parasiticus ATCC 15517 in a yeast-extract sucrose broth (YES) and potato-dextrose agar (PDA) and then exposed to sunlight. The weight of mycelia was decreased to 76.8% in 8 hours and to 66.7% in 168 hours(p<0.05). The total aflatoxin level was significantly decreased to below 50% (46.3% in the YES broth and 49.6% in the PDA) in 8 hours (p<0.05). After 168 hours, a 90.4% degradation of aflatoxin in the YES broth and a 77.2% degradation of aflatoxin in the PDA was observed, respectively (p<0.01). The results showed that the degradation ratios of total aflatoxin level increased with increased exposure time to sunlight. These results indicate that sunlight could be an effective factor in aflatoxin degradation although its effect on mycelia was less pronounced.

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The Effect of Cultural Conditions on the Aflatoxin Production of Aspergillus Flavus ATCC 15517 (배양조건이 Aspergillus flavus ATCC 15517의 Aflatoxin 생성에 미치는 영향)

  • 정덕화;이용욱;김용호;김성영;김종규
    • Journal of Environmental Health Sciences
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    • v.16 no.1
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    • pp.45-53
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    • 1990
  • To investigate the effect of cultural condition on the aflatoxin production of Aspergillus flayus ATCC 15517, mixed culture with Aspergillus niger, better kind of media and size of Cultural vessels were examined. YES medium was better than SLS medium for this study. Small scale test tube culture was showed the possibility to simply examine the growth, total acidity, pH and aflatoxin production during cultivation, and also could reduce the second contamination of aflatoxin B1 from large scale broth cultured. Especially ELISA method is simple, sensitive and specific and therefore well suited to small scale of test tube culture. Mixed culture significantly reduced the aflatoxin production of Aspergillus fiavus ATCC 15517 and showed almost 95% inhibition of that level during the incubtation.

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Survey and Control of the Occurrence of Mycotoxins from Post-harvest Fruits 1. Mycotoxins Produced by Pencillium Isolates from Apple Pear, Citrus and Grape (수확 후 과실류에 발생하는 진균독소의 탐색 및 방베 1. 사과, 배, 감귤, 포도에서 분리한 Penicillium이 생산하는 주요 진균독소)

  • 오소영
    • Plant Disease and Agriculture
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    • v.5 no.2
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    • pp.100-104
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    • 1999
  • A total of 65 isolates of Penicillium were isolated from decayed post-harvest fruits of apple pear citrus and grape. The Penicillium species isolated from the apple were idnetified as P. aurantiogriseum and P. expansum those from the pear were P. crustosum and P. expansum and those from the grape were P. aurantiogriseum and P. expansum, From decayed citrus fruits. P. digitatum and P. italicum were isolated. Citrinin and patulin from these species in the YES(yeast extract sucrose) broth were extracted with ethyl acetate and purified by thin-layer chromatography(TLC) and high performance liquid chromatography(HPLC) Among 51 isolates of Penicillium from apple pear and grape 7 isolates produced citrinin 13 isolates produces patulin and 12 isolates produced citrinin and patulin also. All 14 isolates of Penicillium from citrus produced only patulin.

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Effect of Panax ginseng on the Growth and Production of Aflatoxin by Aspergillus flayus (인삼이 Aspergillus flayus의 생육 및 Aflatoxin 생성에 미치는 영향)

  • 이창숙;김종규
    • Journal of Environmental Health Sciences
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    • v.20 no.4
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    • pp.90-97
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    • 1994
  • This study was performed to investigate the effect of the Panax ginseng C. A. Meyer on the growth and production of aflatoxin by Aspergillus flayus ATCC 15517. Asp. fiavus with 10$^6$ conidia was incubated at 30$\circ$C for 7 days on YES broth containing 0.1%, 0.5%, and 1.0% of ginseng extract. After incubation, dry mycelial weight, pH, and production of aflatoxin were investigated. The results were as follows:There was no significant difference in dry mycelial weight by the addition of 0.1% and 0.5% ginseng extract. However, it was decreased to the rate of 13.7% by the addition of 1.0% ginseng extract in 7 days. pH changes in cultures were similar regardless of the concentration of ginseng extract. The pH values decreased to minimum in 5 days and again increased. Aflatoxin production was reduced as the concentration of ginseng extract increased. When compared to the control, the production of total aflatoxin significantly reduced to 56.7%, 54.0%, 53.3% in the media of 0.1%, 0.5% and 1.0% of ginseng extract, respectively. No significant difference was observed among ginseng extract groups.

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Effect of Lactobacillus casei and a Fermented Milk on the Growth and Aflatoxin Production of Asperillus Parasiticus (유산균과 그 발효유가 Asperigllus parasiticus의 생육과 Aflatoxin 생성에 미치는 영향)

  • 김종규;이용욱
    • Journal of Food Hygiene and Safety
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    • v.13 no.2
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    • pp.164-170
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    • 1998
  • In this study a commercial fermented milk produced in Korea and a Lactobacillus strain used for the product (L. casei) were found to affect mold growth and inhibit aflatoxin production by Aspergillus parasiticus ATCC 15517. Aflatoxins were determined using an HPLC system that consisted of a $C_{18}$ column and a fluorescence detector. When the fermented milk was added to the yeast-extract broth the levels of aflatoxin $B_{1}\;and\;G_{1}$ significantly decreased by 48.6~58.1% and 29.8~34.2%, respectively (p$B_{1}\;and\;G_{1}$ were found in comparison with the control (monoculture). L. casei was found to be very inhibitory to the growth of A. parasiticus for 5 days, but no significant difference of mycelial weight was observed between the mixed culture and control at the end of incubation. The pH values of the culture broth in mixed culture were observed to be significantly lower than those in monoculture (p

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