• Title/Summary/Keyword: Zebrafish

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Alcohol Impairs learning of T-maze Task but Not Active Avoidance Task in Zebrafish

  • Yang, Sunggu;Kim, Wansik;Choi, Byung-Hee;Koh, Hae-Young;Lee, Chang-Joong
    • Animal cells and systems
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    • v.7 no.4
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    • pp.303-307
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    • 2003
  • The aim of this study is to investigate whether alcohol alters learning and memory processes pertaining to emotional and spatial factors using the active avoidance and T-maze task in zebrafish. In the active avoidance task, zebrafish were trained to escape from one compartment to another to avoid electric shocks (unconditioned stimulus) following a conditioned light signal. Acquisition of active avoidance task appeared to be normal in zebrafish that were treated with 1% alcohol for 30 min for 17 days until the end of the behavioral test, and retention ability of learned behavior, tested 2 days later, was the same as control group. In the T-maze task, the time to find a reservoir was compared. While the latency was similar during the 1 st training session between control and alcohol-treated zebrafish, it was significantly longer in alcohol-treated zebrafish during retention test 24 h later. Furthermore, when alcohol was treated 30 min after 2nd session without prior treatment, zebrafish demonstrated similar retention ability compared to control. These results suggest that chronic alcohol treatment alters spatial learning of zebrafish, but not emotional learning.

Inheritance of Golden Coloration in the Zebrafish, Danio rerio (Zebrafish (Danio rerio)의 체색 변이에 관한 유전 분석)

  • 송춘복;이병문
    • Journal of Aquaculture
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    • v.10 no.4
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    • pp.395-402
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    • 1997
  • The study has been conducted in order to understand the inheritance of body color in the wild type zebrafish (zebra danio), Danio rerio, and its golden mutant (golden danio). The body color was also studied to determine the effect of golden coloration on the survival rate of zebrafish eggs and larvae up to 15 days after fertilization. Reciprocal monohybrid crosses between the wild and the golden type of zebrafish indicated that golden coloration was controlled by a single gene which had two alleles. Transmission of these alleles from parents to their progenies followed the principles of dominance and segregation based on Mendelian inheritance. Similar results from the reciprocal crosses implied that a locus for golden coloration was located on an autosomal chromosome. On the other hand, average survival rates from four different types of mating between, and within, zebra and golden danio suggested that golden coloration seemed to be associated with the survival rate of zebrafish, especially in its early embryonic stage. This indicated that homozygous recessive golden mutation was likely to weaken the golden danio's chance of survival.

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The difference of metabolic profile between male and female zebrafish

  • Yoon, Dahye;Choi, Jin;Choi, Hyeonsoo;Kim, Suhkmann
    • Journal of the Korean Magnetic Resonance Society
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    • v.20 no.1
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    • pp.13-16
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    • 2016
  • Various experiments using zebrafish have been highlighted recently in the scientific community. Because it is possible to conduct practical experiment from various neurological research to area of genetic study or toxicity experiment. However, gender difference effects are nearly not considered. If the gender differences of zebrafish are considered it is possible to obtain more accurate data. In this study, zebrafish which have different genders were compared each other with NMR-based metabolomics. The extracts of male and female zebrafish were measured by 600 MHz NMR spectrometer. Statistical analysis and target profiling were conducted. As a result, muscle related metabolites were observed in male zebrafish and nerve related metabolites were observed in female zebrafish.

Mind Bomb1 and DeltaD are Localized into Autophagosome after Endocytosis in Zebrafish during Neurogenesis

  • Kim, Min-Jung
    • Development and Reproduction
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    • v.15 no.3
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    • pp.215-221
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    • 2011
  • Endocytosis of the Notch ligand, DeltaD, by mind bomb1 is indispensable for activation of Notch in cell fate determination, proliferation, and differentiation during zebrafish neurogenesis. Loss of mind bomb1 activity as an E3 Ubiquitin ligase causes the accumulation of deltaD at the plasma membrane and results in the ectopic neurogenic phenotype by activation of Notch in early zebrafish embryogenesis. However, the regulatory mechanism of deltaD during neurogenesis is not identified yet. This study aims to analyze the pathway of mib1 and deltaD after endocytosis in vivo during zebrafish embryogenesis. Mind bomb1 and deltaD are co-localized into autophagosome and mutant form of mind bomb1 fails to cargo deltaD into autophagosomes. These findings suggest that mind bomb I mediates deltaD regulation by autophagy in an ubiquitin-dependent manner during zebrafish embryogenesis.

1,8-cineole protected human lipoproteins from modification by oxidation and glycation and exhibited serum lipid-lowering and anti-inflammatory activity in zebrafish

  • Cho, Kyung-Hyun
    • BMB Reports
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    • v.45 no.10
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    • pp.565-570
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    • 2012
  • We recently reported that a water extract of laurel or turmeric, 1,8-cineole enriched fractions, showed hypolipidemic activity in the zebrafish model. Therefore, the present study investigated the cineole's anti-oxidant and anti-inflammatory activities in lipoprotein metabolism in vitro and in vivo. Cineole had inhibitory effects on cupric ion-mediated oxidation of lipoproteins in general, while simultaneously enhancing ferric ion removal ability in high-density lipoprotein (HDL). Hypercholesterolemia was induced in zebrafish using cholesterol-feeding treatment, 4% cholesterol, for 3 weeks. After feeding with or without the addition of cineole, the results revealed that cineole possessed lipid-lowering and anti-inflammatory activities in hypercholesterolemic zebrafish. In addition, serum amyloid A and interleukin-6 levels were lowered and lipid accumulation was decreased in the liver. Conclusively, 1,8-cineole was found to have anti-oxidant activities in lipoprotein metabolism both in vitro and in vivo with simultaneous reduction of lipid accumulation in the liver of zebrafish.

Application of zebrafish as a model for evaluation of vaccine efficacy against Philasterides dicentrarchi (Ciliphora: Scuticociliatia)

  • Lee, Eun-Hye;Kim, Ki-Hong
    • Journal of fish pathology
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    • v.22 no.1
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    • pp.45-52
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    • 2009
  • Zebrafish was firstly applied to an experimental model for scuticociliatosis caused by Philasterides dicentrarchi, a facultative parasitic ciliate in cultured marine fish. The susceptibility of zebrafish to infection of P. dicentrarchi was assessed by intraperitoneal injection of the ciliates, which produced typical symptoms of scuticociliatosis and significant mortality. The potential use of zebrafish as a model to evaluate the vaccine efficacy against scuticociliatosis was analyzed by immunization of zebrafish with the ciliates lysate. Furthermore, the effect of different adjuvants, such as Quillaja saponin (QS), Montanide, and Freund’s incomplete adjuvant (FIA) on the protective efficacy of the vaccine was investigated. Groups of zebrafish injected with QS or Montanide alone showed higher survival of fish against challenge test compared to control fish. The results suggest that adjuvant-mediated enhancement of innate immune responses play important roles in protection of fish against scuticociliatosis. The considerably high survival in the fish immunized with the antigen alone indicates that the ciliate lysate itself is highly immunogenic to zebrafish, which can elicit protective immune responses. The protective potential of the antigen, ciliate lysate, was enforced through combined administration with adjuvants including QS, Montinide and FIA. No or low mortalities in the groups of fish immunized with the antigen plus adjuvants suggests that the adaptive immune responses of zebrafish might be accelerated by the adjuvants or the protective potential of the antigen and adjuvants might synergistically interact. In spite of several shortcomings such as difficulties in sampling of serum and leucocytes enough to routine immunological analyses, zebrafsih might be the most convenient experimental animal for scuticociliatosis.

Anxiolytic effects of an acetylcholinesterase inhibitor, physostigmine, in the adult zebrafish

  • Cho, Han-Eul;Lee, Chang-Joong;Choi, Ji-Seon;Hwang, Jin-Soo;Lee, Yun-Kyoung
    • Animal cells and systems
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    • v.16 no.3
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    • pp.198-206
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    • 2012
  • Anxiety in zebrafish can be determined by examining their bottom-dwelling and light-avoidance behavior. This study determines the effects of physostigmine and scopolamine on anxiety in zebrafish by measuring swimming frequency for three horizontal layers and three vertical columns of a water test tank illuminated by a light source located above the central surface of the tank. In the 1 h session, zebrafish in the control group preferred the bottom layer the most and the center column the least. Zebrafish treated with 2-20 ${\mu}M$ physostigmine were more likely to prefer the to layer than controls, and there were significant pairwise differences between physostigmine-treated zebrafish and controls, indicating the anxiolytic effect of physostigmine. Further, 10 and $20{\mu}M$ physostigmine-treated zebrafish no longer avoided the center column. Scopolamine had no anxiolytic effect on bottom-dwelling and light-avoidance behaviors but suppressed the anxiolytic effect of physostigmine. In terms of their preference for various zones formed by layers and columns, zebrafish in the control group preferred the bottom left and right zones the most. Physostigmine had a positive effect on the preference for the top center zone, which was suppressed by scopolamine pretreatment. The results suggest that the level of anxiety in zebrafish can be reduced by activating acetylcholinergic neurotransmitter systems, which is mediated in part by muscarinic receptors.

Recovery Effect of Flavonoids from Morus alba Fruits on Alloxan-induced Pancreatic Islet in Zebrafish (Dinio rerio) (오디 Flavonoid의 alloxan 처리 zebrafish 췌장섬에 대한 회복효과)

  • Seo, Kyeong-Hwa;Nam, Youn-Hee;Kim, Young-Eon;Hong, Eock-Kee;Hong, Bin-Na;Kang, Tong-Ho;Baek, Nam-In
    • Journal of Applied Biological Chemistry
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    • v.58 no.1
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    • pp.51-54
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    • 2015
  • Flavonoids from Morus alba fruits were evaluated for recovery effect on the damage of alloxan-induced pancreatic islet in zebrafish. Alloxan treatment on the zebrafish embryo surely decreased the pancreatic islet size (p <0.001). Rutin, isoquercetin, and quercetin very significantly recovered the size (p <0.001) and the fluorescence intensity of pancreatic islet.

Isoprocarb induces acute toxicity in developing zebrafish embryos through vascular malformation

  • Park, Hahyun;Song, Gwonhwa;Lim, Whasun
    • Journal of Animal Reproduction and Biotechnology
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    • v.36 no.1
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    • pp.17-24
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    • 2021
  • In this study, the potential toxicity of isoprocarb was demonstrated using zebrafish embryos. We treated isoprocarb (0, 29, and 58 mg/L) to the zebrafish embryos for 72 h then, we estimated morphological changes and apoptotic cell numbers. The increasing extent of apoptosis from the anterior to posterior region of developing zebrafish larvae was correlated with toxicity in the overall development process, including growth and normal organ formation. The appearance of abnormalities in the isoprocarb-treated groups in comparison to normal developing zebrafish larvae was verified using quantitative image analysis based on ImageJ software program. The vascular system comprising a complex interconnection of blood vessels was visualized in vessel-fluorescent transgenic zebrafish (fli1:eGFP). The main vasculature was malformed on isoprocarb treatment, and this was also related to cardiac defects. Taken together, normal embryonic development in zebrafish was interrupted owing to the acute toxicity of isoprocarb.

In vivo Screening of Herbal Extracts on High Glucose-induced Changes in Hyaloid-Retinal Vessels of Zebrafish (고혈당으로 유도된 제브라피쉬 당뇨망막병증 모델에서 약용식물의 효능 평가)

  • Lee, Yu-Ri;Jung, Seung-Hyun;Lee, Ik Soo;Kim, Joo Hwan;Kim, Young Sook;Kim, Jin Sook
    • Korean Journal of Pharmacognosy
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    • v.50 no.1
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    • pp.25-31
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    • 2019
  • The zebrafish (Danio rerio) is an established model organism for several pathophysiological conditions which are related to human diseases. In this study, we tested the preventive effect of eight herbal extracts, which show the inhibitory effect of advanced glycation end products (AGEs) or aldose reductase (AR) in our previous study, on high glucose (HG)-induced retinal vessel dilation in larval zebrafish and analyzed the change of hyaloid vasculature. HG-induced zebrafish hyaloid vasculatures were significantly increased in the thickness compared to untreated zebrafish (P<0.001, n=6~10). Eight herbal extracts were found to have significant retinal vessel dilation on the inhibitory activity. Particularly, Brandisia hancei (twigs and fruits), Castanopsis orthacantha (leaves and twigs), Litsea japonica (leaves and twigs), Spenceria ramalana (whole plant), and Synedrella nodiflora (leaves and stems) showed potent inhibitory activity against retinal vessel dilation in HG-induced larval zebrafish.