• Title, Summary, Keyword: acetylcholinesterase

Search Result 463, Processing Time 0.039 seconds

Production and Purification of Acetylcholinesterase Inhibitor from Pseudomonas sp960903 (Pseudomonase sp. 960903에 의한 acetylcholinesterase 억제제의 생산 및 정제)

  • 김경자
    • Microbiology and Biotechnology Letters
    • /
    • v.28 no.6
    • /
    • pp.322-328
    • /
    • 2000
  • To screen agent for the treat-ment of Alzhimers Disease several strains of bacteria producing acetylcholinesterase inhibitor ware isolated from soil. Strain 960903 showed strong acetylcholinesteras inhibitory activity and low butyrylcholinesterse inhibitory activity. The strain 960903 was identified as Pseudomonas sp. Acetylcholinesterase inhibitor ws highly achieved in fermentation medium containing soluble starch 3.0%, glycerol 1.0%, pharmamedia 0.5%, KCI 0.3%, $CaCO_3$ 0.2%, MgS $O_4$..$7H_2$O 0.05%, $KH_2$$PO_4$ 0.05%(pH6.5) at $30^{\circ}C$ for 4 days. Acetylcholinesterase inhibitor was purified by Diaion WA-30($OH^{-}$) column charomatography and cellulose column chromatography. Acetylcholinesterase inhibi-tor showd the maximum wavelength at 205 nm and was soluble in water, acetic acid, ethanol, methanol and dime-thyl sulfoxide. The concentration of 50% inhibition($IC_{50}$) of inhibitor against acetylcholinesterase was 25$\mu\textrm{g}$/ml. The inhibitor was inactivated on heating ar $100^{\circ}C$ fro 15 min and more stable in acidic region than alkaline region.n.

  • PDF

An Acetylcholinesterase Inhibitor Isolated from Corydalis Tuber and Its Mode of Action (현호색의 Acetylcholinesterase 활성 저해 성분 및 그 작용기전)

  • Hwang, Se-Young;Chang, Young-Pyo;Byun, Soon-Jung;Jeon, Mee-Hee;Kim, Young-Choong
    • Korean Journal of Pharmacognosy
    • /
    • v.27 no.2
    • /
    • pp.91-95
    • /
    • 1996
  • In the course of searching for acetylcholinesterase inhibitors from crude drugs, it was found that total MeOH extract of Corydalis Tuber showed significant inhibitory effect on acetylcholinesterase. To isolate acetylcholinesterase inhibitors from Corydalis Tuber, total MeOH extract of the the crude drug was subjected to activity guided fractionation. The MeOH extract was suspended in water and fractionated with methylene chloride and subjected to acid-base fractionation. Silica gel column chromatography of the basic fraction which showed significant inhibitory effect on acetylcholinesterase was carried out and 5 subfractions (1-5) were obtained. From subtraction 4, compound I was isolated. The structure of isolated compound I was identified by spectroscopic parameters of $^1H-NMR$, $^{13}C-NMR$, EI-MS and FAB-MS. The compound I was identified as berberine. It was found from the Lineweaver-Burk plot that berberine was a reversible and specific inhibitor of acetylcholinesterase having 90% inhibitory effect at the concentration of $2.5{\mu}M$.

  • PDF

Chemical Constituents and Their Acetylcholinesterase Inhibitory Activity of Underground Parts of Clematis heracleifolia (조희풀(Clematis heracleifolia) 지하부의 성분과 Acetylcholinesterase억제 활성)

  • Kim, Mi Ae;Kim, Myong Jo;Chun, Wanjoo;Kwon, Yongsoo
    • Korean Journal of Pharmacognosy
    • /
    • v.46 no.1
    • /
    • pp.6-11
    • /
    • 2015
  • To clarify chemical constituents of Clematis heracleifolia, isolation and structure elucidation of the underground parts of C. heracleifolia were performed. Five compounds were isolated from $CHCl_3$ and n-BuOH soluble fraction of this plant. On the basis of spectral and physico-chemical data, the structure of isolated compounds were identified as coniferyl alcohol (1), scoparone (2), (+)-lariciresinol (3), phytosterols (4), and daucosterol (5), respectively. All compounds are isolated from this plant for the first time. To evaluate anti-acetylcholinesterase activity of the isolated compounds, compounds 1, 2, 3, and 5 were tested inhibition activity against acetylcholinesterase. Among tested compounds, daucosterol (5) showed acetylcholinesterase inhibitory activity with $IC_{50}$ value of $6.1{\mu}M$.

Antioxidant Activity and Acetylcholinesterase Inhibitory Activity of Ark shell (Scapharca broughtonii) (피조개의 항산화 활성과 Acetylcholinesterase 저해 활성)

  • Kim, Jeong-Been;Kim, Jae-Min;Lee, Young-Min;Baek, In-Seok;Lee, Seung-Cheol
    • Korean journal of food and cookery science
    • /
    • v.30 no.2
    • /
    • pp.212-218
    • /
    • 2014
  • Ark shell (Scapharca broughtonii; Korean name, pijogae) is one of the most widely cultivated and consumed shellfishes in Korea. The purpose of this study was to evaluate the antioxidant activity and acetylcholinesterase inhibitory activity of ark shell. After preparing the methanol extract of ark shell powder, the extract was subsequently fractionated by hexane, diethyl ether, ethyl acetate and water. The antioxidant activity evaluated by DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power was relatively higher in the water fraction; however, the activity was spread out in all fractions. Acetylcholinesterase inhibitory activity was the highest in the diethyl ether fraction. Taken together with the results of both antioxidant and acetylcholinesterase inhibitory activities, it can be suggested that different kinds of physiological compounds were contained in the ark shell.

Inhibition of Acetylcholinesterase Activity on the Organophosphorus and Carbamate Pesticides (유기인계 및 Carbamates 농약에 의한 Acetylcholinesterase 활성의 저해)

  • 김정호;김영호
    • Journal of Environmental Science International
    • /
    • v.7 no.1
    • /
    • pp.52-56
    • /
    • 1998
  • The effects of organophosphorus and carbamate pesticides were examined inhibition of the acetylcholinesterase activity in the chicken brain with enzyme-inhibition methods. The acetylcholinesterase activity in chicken brain determined by the Ellman method was 167 mmol/min/g protein. The optimum pH of acetycholinesterase was 8.2. $pl_{50}$ of acetycholinesterase by some organophosphorus were 3.80M of phosphorodlthioate, 4.04M of phosphorothioate, 6.33M of phosphate, and 6.60M of phosphrothiolate. pluto of acetycholinesterase by some carbamates were 5.1 OM of XMC, 5. 90M of carbofuran, 6.16M of isoprocarb, 6.30M of carbaryl, 6.47M of BPMC, and 6.77M of propoxur. pluto of carbamates selected was similar to that of phosphorothioate and phosphate organophosphates.

  • PDF

Acetylcholinesterase Inhibitors from the Stem of Zea mays

  • Sim, Jae Young;Kim, Mi Ae;Kim, Myong Jo;Chun, Wanjoo;Kwon, Yongsoo
    • Natural Product Sciences
    • /
    • v.20 no.1
    • /
    • pp.13-16
    • /
    • 2014
  • Five compounds were isolated from the stem of Zea mays. Based on spectral data, they were identified as 4-hydroxybenzaldehyde (1), N-trans-p-coumaryl tyramine (2), N-trans-ferulyl tryptamine (3), N-(p-coumaryl) serotonine (4), and N-(p-coumaryl)-tryptamine (5). All isolates were evaluated in vitro for their inhibitory activity on acetylcholinesterase. Among tested compounds, compounds 2 - 5 exhibited acetylcholinesterase inhibitory activity, with $IC_{50}$ values of 125, 60.4, 183.5 and 53.3 ${\mu}M$, respectively. Compound 1 did not show acetylcholinesterase inhibitory activity in the present study.

Effect of Papaverine on Acetylcholinesterase in Rat Brain

  • Park, Eun-Hee;Eun, Chung
    • Archives of Pharmacal Research
    • /
    • v.12 no.1
    • /
    • pp.34-38
    • /
    • 1989
  • Papaverine administration produced significant increases in acetylcholinesterase activity in cerebral cortex and striatum of rat brain. Two related compounds, tetrahydropapaverine and tetrahydropapaveroline, also gave similar effects. However, their actions seem to be indirect because papaverine has no in vitro effect on the enzymatic activity.

  • PDF

Production of Anti-dementia Acetylcholinesterase Inhibitor from Pleurotus ostreatus (Heuktari) and Inhibitory Effect on PC12 Neuron Apoptosis (흑타리버섯으로부터 항치매성 Acetylcholinesterase 저해물질의 생산 및 PC12 신경세포사 저해 효과)

  • Han, Sang-Min;Kim, Ji-Yoon;Lee, Jong-Soo
    • The Korean Journal of Mycology
    • /
    • v.47 no.4
    • /
    • pp.337-346
    • /
    • 2019
  • To develop a new antidementia acetycholinesterase (AChE) inhibitor from edible mushrooms, the inhibitory effects on AChE of water and ethanol extracts from various edible mushrooms were measured. Among the tested compounds, 70% ethanol extracts from Tremella fuciformis showed the highest AChE inhibitory activity, at 25.3% (IC50: 9.9 mg). Water extracts from the fruiting body of Pleurotus ostreatus (Heuktari) showed AChE inhibitory activity of 20.2% (IC50: 12.4 mg). However, the yield (40.8%) from Pleurotus ostreatus (Heuktari) was higher than that from Tremella fuciformis (5.0%). Therefore, we selected Pleurotus ostreatus (Heuktari) as the most promising candidate for a mushroom containing anti-dementia AChE inhibitors. The AChE inhibitor from Pleurotus ostreatus (Heuktari) was optimally extracted when its fruiting body was treated with water for 6 h at 30℃. The anti-dementia effects of the partially purified AChE inhibitor from Pleurotus ostreatus (Heuktari) were observed in PC12 nerve cells.

The nervous system of Fibricola seoulensis by acetylcholinesterase histochemistry (Acetylcholinesterase 조직화학법으로 관찰한 Fibricola seoulensis 신경계의 분포)

  • 전은우;김창환
    • The Korean Journal of Parasitology
    • /
    • v.31 no.4
    • /
    • pp.321-330
    • /
    • 1993
  • F. seoulensis were obtained from artificially infected albino rats at 3, 4, 5, 6, 7 days after infection. The worms and metacercariae were washed in physiological saline solution, and fled with 10% neutral formalin. The acetylcholinesterase (AchE) stained by one histochemistry using acetylthiocholine iodide as substrate. Eserine, ism-OMPA and BW284C51 were used as inhibitors of AchE. The nervous system consists of three pairs longitudinal nerve trunks interconnected with excretory plexus in posterior half, and phinmc and oral sucker in anterior half of metacercariae and adults. The longitudinal nerve trunks are interconnected with transverse commissures and numerous circular commissures. Considerable numbers of circular commissures are interconnected with longitudinal nerve trunks tying on the surface of the worms. At each stage of juvenile worms, AchE and nonspecific cholinesterase activites were observed in the oral sucker, ventral sucker, pharynx and nerve system. Isoxymes of AchE in f seoderuts were separated into the two bands, 69 kDa and 132 kDa. The major band was 69 kDa.

  • PDF

Antioxidant and Acetylcholinesterase Inhibitory Activities of Subcritical Water Extracts from Houttuynia cordata Thunb (어성초의 아임계수 추출물의 항산화 활성 및 Acetylcholinesterase 저해 활성)

  • Jo, Eun-Kyung;Lee, Seung-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.40 no.10
    • /
    • pp.1391-1396
    • /
    • 2011
  • Houttuynia cordata Thunb powder (0.05 g) was placed in a stainless vessel containing 10 mL of water, and subcritical water (SCW) extraction was carried out at various temperatures (50, 100, 200, and $300^{\circ}C$) for 10, 30, and 60 min. SCW treatment significantly affected physiologically important compounds, antioxidant activity, and acetylcholinesterase inhibitory activity of H. cordata Thunb extracts. H. cordata Thunb extracted at $300^{\circ}C$ for 30 min had the highest quercetin content, DPPH radical scavenging activity, total phenolic content, and acetylcholinesterase inhibitory activity, whereas the extract at $100^{\circ}C$ for 10 min possessed the highest flavonoid content. The highest ABTS radical scavenging activity was found in H. cordata Thunb extracted at 200 and $300^{\circ}C$ for 60 min. These results indicate that SCW extraction might be a useful processing method for obtaining valuable materials from H. cordata Thunb.