• Title, Summary, Keyword: alkali-tolerant Bacillus sp

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Properties of Promoters Transferred to the Donor Strain, Alkali-tolerant Bacillus sp. YA-14. (공여 균주인 알카리 내성 Bacillus속에 도입된 Promoter 의 특성)

  • 유주현;구본탁;정용준
    • Microbiology and Biotechnology Letters
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    • v.17 no.3
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    • pp.188-192
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    • 1989
  • The promoters from akali-tolerant Bacillus sp. YA-14 chromosomal DMA cloned in B. subtilis using pPL703 were stably transferred to the donor strain. In alkali-tolerant Bacillus sp., the promoters revealed similiar properties with in B. subtilis but were preyed to be more efficient than in B. subtilis comparing with pPL708. Alkali-tolerant Bacillus sp. harboring the recombinant plasmid, p-l2Bl, was abnormally more inducible with chloramphenicol than B. subtilis haying the plasmid. Therefore the host-vector system using this recombinant plasmid and alkali-tolerant Bacillus sp. was expected to be more available.

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Expression of Developmentally Regulated Promoter of Alkali-tolerant Bacillus sp. YA-I4 (알칼리 내성 Bacillus sp. YA-14에서 유래된 생육단계 조절 promoter의 발현)

  • 박영서;구본탁;박희경;유주현;김진만
    • Microbiology and Biotechnology Letters
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    • v.18 no.4
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    • pp.429-432
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    • 1990
  • The promoter isolated from chromosomal DNA of an alkali-tolerant Bacillus sp. YA-14 was subcloned and biochemically characterized. Also the relationships between the promoter activity and sporulation were investigated. In alkali-tolerant Bacillus sp. and Bacillus subtilis, the activity of promoter began to increase at the onset of sporulation with the same mode, and repressed in the presence of 1.0% (wtv) glucose. Among five spoO genes, three epoO genes (spoOB, spoH, spoOJ) were required for promoter expression.

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Studies on the Properties of the Promoter from Alkali-Toleran t Bacillus sp. (알칼리 내성 Bacillus sp.속 유래 Promoter의 발현특성)

  • 박희경;박영서;김진만;유주현
    • Microbiology and Biotechnology Letters
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    • v.19 no.1
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    • pp.21-24
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    • 1991
  • The promoter isolated from an alkali-tolerant Hwillus sp. chromosomal DNA was subcluned. The activity of promoter in B, subtilis and alkali-tolerant Bacillus sp. began to increase at the early stage. of spore formation. In the presence of 1% glucose, the promotcr activity repressed and was recovered by ;tddition of c-GMP in the medium.

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Cloning of Pectate Lyase Gene of Alkali-tolerant Bacillus sp. YA-14 and Its Expression in Escherichia coli (알카리 내성 Bacillus sp. YA-14의 Pectate Lyase 유전자의 클로닝과 발현)

  • Yu, Ju-Hyun;Park, Yoon-Suk;Kim, Jin-Man;Kong, In-Soo;Chung, Yong-Joon
    • Microbiology and Biotechnology Letters
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    • v.16 no.4
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    • pp.316-319
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    • 1988
  • Pectate Lyase (PL) was cloned from alkali-tolerant Bacillus sp. YA-14 into Escherichia coli MB1000 by inserting HindIII-generated DNA fragment into the HindIII site of pBR322 and then screening recombinant transformant for the ability to hydrolyze sodium polypectate on agar plate, The recombinant plasmid, called pYPC29, was isolated, and the size of the cloned HindIII fragment was found to be 1.6 kb. The PL gene was stablely maintained and expressed efficiently in Escherichia coli. The Pt accumulated largely in the periplasmic space of Escherichia coli clones.

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Cloning of Promoters from Alkali-tolerant Bacillus sp. (알카리 내성 Bacillus속 Promoter의 Cloning)

  • 유주현;구본탁;공인수;정용준;박영서
    • Microbiology and Biotechnology Letters
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    • v.16 no.2
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    • pp.126-130
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    • 1988
  • Promoters of an alkali-tolerant Bacillus sp. isolated from soil have been cloned in Bacillus subtilis using promoter probe vector pPL703. The CAT specific activity of a clone harboring the strongest promoter activity among these transformants was 8.01. This activity was 2.5 times higher than that of Bacillus subtilis harboring expression vector pPL708 and was increased after the end of the logarithmic growth phase. In the 2.8kb of inserted DNA fragment, BamHI and Sal I recognition sites were located.

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Dilute Solution Properties of Biopolymer Produced by Alkali-Tolerant Bacillus sp. (알칼리 내성 Bacillus Sp.에 의한 생물 고분자의 희석용액 특성)

  • Lee, Shin-Young;Kim, Jin-Young
    • Journal of Industrial Technology
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    • v.20 no.A
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    • pp.39-44
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    • 2000
  • Highly viscous biopolymer from alkali-tolerant Bacillus sp. was purified and its solution properties were investigated. The intrinsic viscosities for crude biopolymer and biopolymers purified by dialysis or CPC(cetylpyridinium chloride) treatment were 58.24, 73.60 and 42.18 dL/g, respectively. The intrinsic viscosity of biopolymer showed the maximum value at the neutral pH but it was decreased remarkably at the alkaline or acidic pH. Biopolymer exhibited the property of polyelectrolyte, showing the sharp decrease of intrinsic viscosity by the addition of NaCl. Intrinsic viscosity of dilute solution at the low NaCl concentration was exponentially dependent on temperature and its temperature dependency was increased with NaCl concentrations. The chain stiffness, coil overlap parameter, and critical concentration were 0.09, 5.25 and 0.07g/dL, respectively. Temperature dependency on intrinsic viscosity of biopolymer solution was different each other at $45^{\circ}C$. Flow activation energies at temperatures above $45^{\circ}C$ were constant, while those at temperatures below $45^{\circ}C$ increased with increase of added NaCl concentration.

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Nucleotide Sequence and Analysis of a Xylanase gene (xynS) from Alkali-tolerant Bacillus sp. YA-14 and Comparison with Other Xylanases

  • Yu, Ju-Hyun;Park, Young-Seo;Yum, Do-Young;Kim, Jin-Man;Kong, In-Soo
    • Journal of Microbiology and Biotechnology
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    • v.3 no.3
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    • pp.139-145
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    • 1993
  • The nucleotide sequence of the xylanase gene (xynS) from alkali-tolerant Bacillus sp. YA.14 was determined and analyzed. A 639 base pairs open reading frame for xynS gene was observed and encoded for a protein of 213 amino acids with a molecular weight of 23, 339. S1 nuclease mapping showed that the transcription initiation site of the xynS gene did not exist in the cloned DNA. Ribosome binding site sequence with the free energy of -18.8 Kcal/mol was observed 8 base pairs upstream from the initiation codon, ATG. The proposed signal sequence consisted of 28 amino acids, of which 3 were basic amino acid residues and 21 were hydrophobic amino acid residues. When the amino acid sequences of xylanases were compared, Bacillus sp. YA-14 xylanase showed 48% homology with Bacillus sp. YC-335 xylanase and 96% homology with xylanases from B. subtilis and B. circulans.

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Cloning and Expression of a Xylanase Gene from Alkali-tolerant Bacillus sp. YA-14 in Escherichia coli (알카리 내성 Bacillus sp. YA-14의 xylanase 유전자 cloning)

  • 유주현;박덕철;정용준;공인수
    • Microbiology and Biotechnology Letters
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    • v.17 no.2
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    • pp.154-159
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    • 1989
  • Chromosomal DNA fragments of Bacillus sp. YA-14 isolated from soil as a potent xylan hydrolyzing bacterium, were ligated to a vector plasmid, pBR322, and used to transfer Escherichia coli HB101 cells. The recombinant plasmid pYDC21 was found to enable the transformants to produce xylanase. pYDC21 was found to contain the 3 kb HindIII fragment originated from the Bacillus sp. YA-14 chromosomal DNA by southern hybridization. The optimum temperature and pH for the reaction of xylanse produced by E. coli (pYDC21) were appeared at 50$_7$C and pH 7.0, respectiveiy. the xylanase enzyme was stable between pH 5.0 and 7.0 and maintained stably up to 4$0^{\circ}C$.

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Optimal Condition for Transformation of Alkali-tolerant Bacillus sp. (내알칼리성 Bacillus sp.의형질전환조건)

  • 전용준
    • The Korean Journal of Food And Nutrition
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    • v.12 no.3
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    • pp.233-239
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    • 1999
  • To develop the potential use as new host strain for gene cloning the optimal conditions for transform-ation of alkali-tolerant Bacillus sp. were examined. The Bacillus sp. YA-14 was cultured to late logarith-mic growth phase at 37$^{\circ}C$ in modified SPI medium (pH8.0) containing 0.4% MgSO4 0.5mM CaCl2 1 ml of competent cell was mixed with 0.5$\mu$g of plasmid DNA and incubated with shaking at 37$^{\circ}C$ for 40min. The transformation frequency under the optimal condition was 4.53$\times$10-6 CFU/ml/g plasmid DNA. The electrophresis and stably maintained in the new host.

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Controlled Expression of Promoter from Alkali-tolerant Bacillus sp. DNA in Fed-batch Culture (Fed-batch 배양에 의한 알칼리내성 Bacillus 속 Promoter의 발현조절)

  • 조석철;박혜영;조형용;변유량;김인규
    • Microbiology and Biotechnology Letters
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    • v.18 no.4
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    • pp.406-410
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    • 1990
  • The influence of glucose concentration on cell growth rate and on the expression level of the strong promoter obtained from alkali-tolerant Bacillus sp. YA-14 chromosomal DNA was studied. In fed-batch culture, the promoter activity could be maximized by maintaining a very low level of glucose concentration in the broth and glucose consumption rate below 1.08g/g cell-h. The induction of the promoter was possible by addition of sporulation medium after the cell was grown in growth medium with only low level of CAT activity.

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