• Title, Summary, Keyword: angiotensin II

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Inhibitory Effects of Ginseng Saponins on c-fos mRNA Expression and the Proliferation of Rat Aortic Vascular Smooth Muscle Cells Stimulated by Angiotensin II

  • Choi, Woong;Jung, Jin-Young;Kim, Hun-Sik;Yun, Yeo-Pyo;Park, Jong-Dae;Ahn, Hee-Yul
    • The Korean Journal of Physiology and Pharmacology
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    • v.2 no.2
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    • pp.201-207
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    • 1998
  • To evaluate the possibility that the ginseng saponins could be developed as an anti-arteriosclerotic agent, we examined the inhibitory effects of ginseng saponins (total saponin[TS], panaxatriol[PT], panaxadiol[PD]) on the expression of c-fos mRNA and the proliferation of cultured rat aortic vascular smooth muscle cells (VSMCs) stimulated by angiotensin II (Ang II). TS and PT (1.0 mg/ml) suppressed c-fos mRNA induction in VSMCs stimulated by $10^{-5}$ M Ang II. The order of inhibitory potency was PT>TS. Ginseng saponins ($0.01{\sim}1.0$ mg/ml) inhibited the proliferation of VSMCs stimulated by Ang II in a concentration dependent manner, the inhibitory potency was TS>PT>PD at $0.1{\sim}1.0$ mg/ml. These results suggest that ginseng saponins may suppress Ang II-stimulated proliferation of aortic VSMCs which can be seen in atherosclerosis, hypertension and restenosis.

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Angiotensin I Converting Enzyme Inhibitor Derived from fermented Mussel, Mytilus edulus

  • Je, Jae-Young;Park, Pyo-Jam;Byun, Hee-Guk;Kim, Se-Kwon;Kim, Jong-Bae;Chang, Soo-Hyun
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • pp.191-192
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    • 2002
  • Angiotensin I converting enzyme [EC 3.4.15.11 (ACE) is important in the maintenance of blood pressure. The enzyme removes histidyl-leucine from angiotensin I to form the blood-vessel-constricting octapeptide, angiotensin II, and degrades vasodilative bradykinin in blood vessels and stimulates e release of aldosterone in the adrenal cortex. (omitted)

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The Effect of Coloring Food Additives on the ${\alpha}$-Chymotrypsin Activity (식품착색료가 ${\alpha}$-Chymotrypsin 작용에 미치는 영향)

  • Choi Cheong;Kim, Sang Ok
    • Journal of the Korean Chemical Society
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    • v.21 no.6
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    • pp.445-448
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    • 1977
  • This study was carried out to understand the activity of ${\alpha}$-chymotrypsin, a proteolytic enzyme, to a oligopeptide in the presence of various coloring food additives. 1. The melting point of synthetic oligopeptide, Asp-Arg-Val-Tyr-Ile-His-Pro-D-Ala, ((8-D-Ala) angiotensin Ⅱ) was 210∼$212^{\circ}C$. Chemical formula and molecular weight were $C_{44}H_{67}N_{13}O_{12}{\cdot}2CH_3COOH{\cdot}H_2O$ and 970.08, respectively. 2.The amino acid rations by acid hydrolysis were Asp : 1.01, Arg : 1.03, Val : 1.00, Tyr :40.94, Ile : 1.00, His : 1.05, Pro : 1.04, D-Ala : 1.03. 3. ${\alpha}$-Chymotrypsin cleaved the oligopeptide bond between tyrosine and isoleucine (Tyr-Ile). 4. The addition of food coloring additives as determined by paper chromatogram, did not influence the inhibitory activity of ${\alpha}$-chymotrypsin on oligopeptide, (8-D-Ala) angiotensin II.

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Screening of the Angiotensin II Antagonists from Medicinal Plants (생약의 안지오텐신 II 수용체 결합저해활성 검색)

  • Oh, Won-Keun;Kang, Dae-Ook;Park, Chan-Sun;Ahn, Soon-Cheol;Ko, Hack-Ryong;Kim, Bo-Yeon;Mheen, Tae-Ik;Ahn, Jong-Seog;Lee, Hyun-Sun
    • Korean Journal of Pharmacognosy
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    • v.28 no.1
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    • pp.26-34
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    • 1997
  • The total MeOH extracts of 149 herbal medicines were screened far angiotensin ll receptor antagonistic activity using rat liver membrane fraction. Four medicinal plants- Atractylodes japonica, Evodia officinalis, Scutellaria baicalensis, Machilus thunbergii showed strong angiotensin ll antagonistic activity.

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Characterization of angiotensin II antagonism displayed by KR-31081, a novel nonpeptide AT1 receptor antagonist (안지오텐신 수용체 길항제 KR-31081의 특성에 관한 연구)

  • Lee, Sung-Hou
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.10
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    • pp.2997-3003
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    • 2009
  • The pharmacological profile of KR-31081, a nonpeptide $AT_1$ selective angiotensin receptor antagonist, was investigated by receptor binding studies, functional in vitro assays with rabbit aorta. KR-31081 inhibited the specific binding of $[^{125}I]\;[Sar^1,\;Ile^8]$-angiotensin II to human recombinant $AT_1$ receptor with an 8.6-fold greater potency than losartan ($IC_{50}$: 1.43 and 12.3 nM, respectively), but it did not inhibit the binding of [$^{125}I$] CGP 42112A to human recombinant $AT_2$ receptor ($IC_{50}$: higher than $10{\mu}M$ for both). The Hill coefficient for the competition curve of KR-31081 against $AT_1$ receptor was not significantly different from unity (0.99). Scatchard analysis showed that KR-31081 interacted with human recombinant $AT_1$ receptor in a competitive manner, as with losartan. In functional studies with rabbit aorta, KR-31081 competitively inhibited the contractile response to angiotensin II ($pK_B$ values: 8.66) with 20-70% decrease in the maximum contractile responses, unlike losartan that showed competitive antagonism without any change in the maximum contractile responses to angiotensin II ($pA_2$ values: 7.59). These results suggest that KR-31081 is a highly potent $AT_1$ selective angiotensin II receptor antagonist with a mode of insurmountable antagonism to be developed as the exploratory potential of this compound.

Angiotensin Converting Enzyme Inhibitory Activity of BR-900317 in vivo, and Antihypertensive Effect of its Single Oral Administration on Blood Pressure and Effect on the Renin-angiotensin System in Hypertensive Model Rats (SHR, RHR) (BR-900317의 In vivo에 있어서 Angiotensin 변환효소 저해작용 밀 고혈압 model rat (SHR, RHR)에 있어 단회 경구투여에 의한 강압작용)

  • 장경진;김지한;백우현
    • Biomolecules & Therapeutics
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    • v.1 no.2
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    • pp.220-225
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    • 1993
  • Effect of BR-900317 on the angiotensin I-induced pressor response in pithed rats and the effects of its single oral administration on plasma angiotensin converting enzyme (ACE) activities in normotensive rats and on the cardiovascular system in hypertensive model rats (SHR, RHR), were compared with those of captopril. BR-900317 attenuated the angiotensin I-induced pressor effects in pithed rats. In a single oral dose administration study, BR-900317 inhibited the plasma ACE activities in a dose-dependent fashion. Duration of the action of BR-900317 was similar to that of captopril. BR-900317 produced antihypertensive effect in spontaneously hypertensive rats and dose-dependent antihypertensive effect in 2-kidney Goldblatt hypertensive rats without affecting heart rate. These results suggest that the main mechanism of the antihypertensive effect of BR-900317 is the suppression of angiotensin II production due to the inhibition of the ACE.

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Localization of Angiotensin II in Korean Bovine Follicles and Its Effects on IVM/IVF of Oocytes (한우 난소 내 Angiotensin II의 분포와 이의 첨가가 체외성숙 및 수정에 미치는 영향에 관한 연구)

  • Quen, J. H.;M. H. Lee;S. K. Kim
    • Reproductive and Developmental Biology
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    • v.28 no.1
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    • pp.59-63
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    • 2004
  • 1. The concentrations of Ang. II were 7.20.91 ${\times}$ $10^3$ , 3.80.34 ${\times}$ $10^3$, 3.50.30 ${\times}$ $10^3$, 2.80.22 ${\times}$ $10^3$ pg/ml in bovine follicular fluids from 1∼3 mm, 3∼5 mm, 5∼7 mm and 8∼10 m follicles, respectively. The concentrations of Ang. II decreased in follicular fluids from large follicles. 2. When oocytes were cultured in media containing various concentrations of Ang. II, a higher proportion of oocytes developed to MII stage in medium with 100 ng/ml (79.5%) Ang II compare to that without Ang. II (58.8%). When oocytes from different sizes of follicles were separately cultured in media containing 100 ng/ml Ang. II, maturation rates were higher in oocytes from small and medium follicles those from controls. 3. GSH content in oocytes cultured for 24 hrs in TCM-199 medium containing 10 and 100 ng/ml of Ang. II was also higher than that of oocytes cultured in medium containing 0 or 10 ng/ml Ang. II. When oocytes were cultured in media containing 0, 10, 100, 1,000 ng/ml of Ang. II, the concentrations of GSH were 5.1M, 5.5M, 7.2M, 8.7M, respectively. 4. When oocytes were cultured in media containing various concentrations of 10, 100, 1,000 ng/ml Ang. II, in vitro maturation and developmental rates were 84.0%, 90.0%, 78.0% and 28.0%, 36.0%, 20.0%, respectively. When oocytes were cultured with an addition of Ang. II in media, in vitro maturation rates higher than that of their controls (76.0%).

Pitavastatin Regulates Ang II Induced Proliferation and Migration via IGFBP-5 in VSMC

  • Ha, Yu Mi;Nam, Ju-Ock;Kang, Young Jin
    • The Korean Journal of Physiology and Pharmacology
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    • v.19 no.6
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    • pp.499-506
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    • 2015
  • Angiotensin II (Ang II), a key mediator of hypertensive, causes structural changes in the arteries (vascular remodeling), which involve alterations in cell growth, vascular smooth muscle cell (VSMC) hypertrophy. Ang II promotes fibrotic factor like IGFBP5, which mediates the profibrotic effects of Ang II in the heart and kidneys, lung and so on. The purpose of this study was to identify the signaling pathway of IGFBP5 on cell proliferation and migration of Ang II-stimulated VSMC. We have been interested in Ang II-induced IGFBP5 and were curious to determine whether a Pitavastatin would ameliorate the effects. Herein, we investigated the question of whether Ang II induced the levels of IGFBP5 protein followed by proliferation and migration in VSMC. Pretreatment with the specific Angiotensin receptor type 1 (AT1) inhibitor (Losartan), Angiotensin receptor type 2 (AT2) inhibitor (PD123319), MAPK inhibitor (U0126), ERK1/2 inhibitor (PD98059), P38 inhibitor (SB600125) and PI3K inhibitor (LY294002) resulted in significantly inhibited IGFBP5 production, proliferation, and migration in Ang II-stimulated VSMC. In addition, IGFBP5 knockdown resulted in modulation of Ang II induced proliferation and migration via IGFBP5 induction. In addition, Pitavastatin modulated Ang II induced proliferation and migration in VSMC. Taken together, our results indicated that Ang II induces IGFBP5 through AT1, ERK1/2, P38, and PI3K signaling pathways, which were inhibited by Pitavastatin. These findings may suggest that Pitavastatin has an effect on vascular disease including hypertension.

Angiotensin II는 활성을 조해하는 물질의 검색하는 방법에 대하여

  • 진익열;서정훈
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • pp.188.3-189
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    • 1976
  • Angiotensin II는 peptide (octa)성 hermone으로 취급되고 있으며 강한 blood pressor activity를 나타내고있다. 이와 관련해서 근내 미생물 유래의 혈압강하성물질에 대한 관심이 높아지고 있으며 이것은 여러가지 면에서 흥미를 느끼게 하고있다. 물질을 미생물계에서 검색코저 시도하였으며 여기서 사용한 방법이 이 연구에 적용될 수 있으리라고 생각됨으로 그 방법을 여기에 발표코저 한다.

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Effect of Angiotensin II on Synaptic Transmission in the Rat Subfonical Organ

  • Lee, Ho-Sung;Han, Seong-Kyu;Ryu, Pan-Dong
    • Proceedings of the Korean Biophysical Society Conference
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    • pp.59-59
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    • 1999
  • Circulating substances can directly access the neurons in circumventricular organ (CVO) located on the border of cerebral ventricles. The neurons in CVO are considered to playa pivotal role in blood-brain communication. In subfonical organ (SFO), a CVO in forebrain, angiotensin II (AII) is mown to reduce A type $K^{+}$ current and input resistance, but enhance firing rate.(omitted)

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