• Title, Summary, Keyword: anthocyanin

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Silage Fermentative Quality and Characteristics of Anthocyanin Stability in Anthocyanin-rich Corn (Zea mays L.)

  • Hosoda, Kenji;Eruden, Bayaru;Matsuyama, Hiroki;Shioya, Shigeru
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.4
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    • pp.528-533
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    • 2009
  • The fermentative quality and quantitative change in anthocyanin of anthocyanin-rich corn (Zea mays L.) during storage and in vitro ruminal fermentation were studied. The anthocyanin-rich corn silages in bag silo, drum silo and round bale had good fermentative qualities, such as low pH (5% DM) and butyric acid-free, and its quality was maintained for more than 370 d. The amount of anthocyanin in the anthocyanin-rich corn decreased after ensiling by about 45% (from 3.34 to 1.88 mg/g DM), but stayed constant after day 60. The in vitro incubation of the anthocyanin-rich corn with ruminal fluid revealed little degradation of anthocyanin. These results indicate that the anthocyanin had no negative effect on silage fermentation, and the anthocyanin-rich corn silage is utilizable for practical use as a feedstuff. Our results also demonstrate alteration of the anthocyanin content during storage, and show that anthocyanin-rich corn is a suitable antioxidant source for ruminants because of the high stability of the anthocyanin in ruminal fluid.

Physiology of Anthocyanin Accumulation in Corn Leaves Treated with Metsulfuron-methyl (Metsulfuron-methyl 처리에 의한 옥수수의 Anthocyanin 축적 생리에 관한 연구)

  • Park, E.Y.;Kim, J.S.;Cho, K.Y.;Pyon, J.Y.
    • Korean Journal of Weed Science
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    • v.18 no.3
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    • pp.246-256
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    • 1998
  • The objective of this study was to investigate the cause of anthocyanin accumulation in the corn leaves treated with metsulfuron-methyl. The accumulation of anthocyanin and total sugar was increased with the time after metsulfuron-methyl application and with the greater herbicide concentration as well. The anthocyanin increase was alleviated by the combined treatment of DUMU or the tank-mixture treatment of metsulfuron-methyl, isoleucine and valine. Metsulfuron-methyl could not enhance the anthocyanin formation in the nonchlorophyllous tissue in which photosynthetic carbohydrate production was limited. Upon the exogenous supply of sucrose, however, the contents of anthocyanin was increased in the both chlorophyllous and nonchlorophyllous leaf segment of corn without the herbicide treatment. On the other hand, the herbicide itself did not influence Hill reaction in vitro and photosynthesis electron transport in vivo. The anthocyanin accumulation by metsulfitronmethyl did not occur in the corn mutants deficient in the structural gene of anthocyanin synthesis, but corns deficient in the regulatory gene had the anthocyanin accumulation only in R-r and r-r type. The above results suggest that the purple pigmentation in the corn leaves treated with metsulfuron-methyl is related to the accumulation of photosynthetic carbohydrate which can stimulate the. regulatory gene related to anthocyanin biosynthetic pathway.

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The Formation of Anthocyanin and Growth Response of Ginseng Cell Lin in vitro (기내배양 인삼세포주의 Anthocyanin 형함과 생장반응)

  • An, In-Ok;Park, Ji-Chang;Choe, Gwang-Tae
    • Journal of Ginseng Research
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    • v.13 no.2
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    • pp.143-146
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    • 1989
  • In order to clarify the characteristics of anthocyanln-producing cell line selected by in vitro grown cultures of ginseng (Panax ginseng C.A. Meyer), the calli of anthocyanin-Producing strain were cultured on media supplemented with different concentrations of 2,4-D and sucrose under light or dark condition. The light was found to be essential for anthocyanin synthesis. Anthocyanin synthesis of ginseng cell line was inhibited by the increase of 2,4-D in the medium. On the other hand, sucrose promoted the anthocyanin-production and the optimum concentration of sucrose for the highest production of anthocyanin was 5%. The growth of anthocyanin-producing tell line was best on the medium supplemented with 0.5 mg/l 2,4-D and 5% sucrose.

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Influences of different light sources and light/dark cycles on anthocyanin accumulation and plant growth in Petunia

  • Ai, Trinh Ngoc;Naing, Aung Htay;Kim, Chang Kil
    • Journal of Plant Biotechnology
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    • v.43 no.1
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    • pp.119-124
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    • 2016
  • Anthocyanin accumulation and plant growth were examined in petunia (NT and $T_2$ transgenic plants) by determining the effects of different sources of light and varying light/dark cycles. Red light significantly enhanced anthocyanin content of B-peru+mPAP1; however, it had a negative effect on anthocyanin production in RsMYB1 plants. In general, white light was found to be reasonable for anthocyanin accumulation in all plants. In case of light/dark cycles, application of seven days of light:14 days of dark significantly enhanced anthocyanin content. We found that anthocyanin content detected in transgenic plants expressing anthocyanin regulatory transcription factor genes (B-peru+mPAP1 or RsMYB1) was higher than that in NT plants in all treatments. Plant growth was also influenced by the different light sources and dark/light cycles. Taken together, our results suggest that light source and light/dark cycle play an important role in anthocyanin production and plant growth. The choice of the optimal conditions is also important for anthocyanin production and plant growth depending on NT or transgenic plants carrying anthocyanin regulatory transcription factors.

Hairy Root Culture of Daucus carota for Anthocyanin Production in a Fluidized-bed Bioreactor (유동층 생물반응기에서 anthocyanin 생산을 위한 당근의 모상근 배양)

  • Kim, C.H.;Lee, S.W.;Chung, I.S.
    • Applied Biological Chemistry
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    • v.37 no.4
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    • pp.237-242
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    • 1994
  • Hairy root culture of Daucus carota was investigated for anthocyanin production in a fluidized-bed bioreactor. The growth of hairy roots in this bioreactor increased 2.5 fold while anthocyanin production was lower. However, the anthocyanin production of hairy roots in a fluidized-bed bioreactor was enhanced 2.3 fold in response to the treatment of the fungal elicitor.

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Production of Anthocyanins by Vitis Hybrid Cell Culture (Vitis Hybrid 세포배양에 의한 Anthocyanin의 생산)

  • 박형환;강신권;이재호;최장윤;이윤수;권익부;유주현
    • Microbiology and Biotechnology Letters
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    • v.17 no.3
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    • pp.257-262
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    • 1989
  • The induction of calli from tissues of a grape, Vitis hybrid, and their suspension cultures were performed and various factors were investigated on cell growth and anthocyanin production. It was shown that light intensity and inorganic nitrogen concentration played an important role on anthocyanin production.1:he contents of anthocyanin produced under 10,000 Iux light irradiation were about twice as much as under the dark. The reduction of inorganic nitrogen concentration of MS medium to one to twenty brought about the increase of approximately five to six-fold in total anthocyanin or sixteenfold in anthocyanin content per dry cell weight and addition of nitrate only as inorganic nitrogen source was shown to be the best for anthocyanin production. Miller medium and Gamborg medium were suitable for the anthocyanin production, as well as high concentrations of Co$^{2+}$ and Fe$^{2+}$. And high yield of 40mg anthocyanins per 200m1 flask was obtained by two stage culture using MS medium for the first stage and the modified MS medium for the second stage.

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Production and Identification of Anthocyanin in Hairy Root Cultures of Ginseng (인삼 모상근 배양에 의한 Anthocyanin의 생산과 동정)

  • Ko, Kyeong-Min;Choi, Yang-Soon;Hwang, Baik
    • Journal of Plant Biology
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    • v.37 no.1
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    • pp.85-91
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    • 1994
  • In hairy root cultures of ginseng (Panax ginseng C.A. Meyer) transfonned by Agrobacterium rhizogenes, the effects of light, carbon source and various honnone on hairy root growth and anthocyanin production were investigated. Anthocyanin synthesis began to first occur 5 days after exposure to light, and then maximum yield of anthocyanin was 0.36 mg/g(fr wt) in MS medium after 30 days. Of the nutritional factors concentration of 60 mM nitrogen and sucrose as a carbon source showed marked effects on the growth and anthocyanin productiom MS medium supplemented with 0.5 mg/L IAA was most suitable for the hairy root proliferation, and the best accumulation of anthocyanin was obtained at 1 mg/L IAA treatment (0.41 mg/g, fr wt). Whereas 2,4-D tended to restrain the pigment synthesis. From the isolation and identification of anthocyanin pigments, main anthocyanin in ginseng hairy root was identified as pelargonidin-glucoside.coside.

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Antioxidant, Anticancer and Immune Activation of Anthocyanin Fraction from Rubus coreanus Miquel fruits (Bokbunja) (복분자 안토시아닌 분획의 항산화, 항암 및 면역증진 효과)

  • Choung, Myoung-Gun;Lim, Jung-Dae
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.4
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    • pp.259-269
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    • 2012
  • This study was performed to determined the antioxidant activities, anticancer and immuno-activities of anthocyanin fraction from Rubus coreanus Miquel fruits (Bokbunja). Anthocyanin fraction extracted from Bokbunja revealed the presence of three anthocyanin components, which were tentatively identified as cyanidin 3-O-sambubioside, cyanidin 3-O-xylosylrutinoside and cyanidin 3-O-rutinoside using RP-HPLC/DAD/MS. The anthocyanin fraction from Bokbunja always showed reducing power and high scavenging activities against DPPH, hydroxy radical (OH) and superoxide anion radical ($O_2{^-}$) similar to general synthetic antioxidant and polyphenol compounds from plant origin. Anthocyanin fraction from Bokbunja showed high inhibition on proliferation of LNCaP and A549 cells and did not inhibit the proliferation of other cancer cells. Immuno-activities of Anthocyanin fraction from Bokbunja were investigated, it showed high promotion of human B and T cells growth about 50% and secretion of IL-6 and TNF-${\alpha}$ by treatment after 6 days. Over all, the result of the study suggest that anthocyanin fraction from Bokbunja displays antioxidant activity comparable to that general synthetic antioxidant, also, anthocyanin fraction from Bokbunja are expected to be good candidate for development into source of anticaner and immuno-activator agent in food industry.

Effects of Abscisic acid and Temperature on the Anthocyanin Accumulation in Seedlings of Arabidopsis thaliana

  • Song Ju-Yeun;Kim Tae-Yun;Hong Jung-Hee
    • Journal of Environmental Science International
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    • v.14 no.12
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    • pp.1093-1102
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    • 2005
  • Effects of abscisic acid(ABA) and temperature on the anthocyanin accumulation and phenylalanine ammonia Iyase(PAL) activity were investigated in seedlings of Arabidopsis thaliana. In time course study, exogenous application of ABA $(50-1000\;{\mu}M)$ led to a noticeable increase in anthocyanin pigments which persisted over the following 5 days. Anthocyanins increased in concert with the chlorophyll loss. The activity of PAL, a key enzyme in the phenylpropanoid pathway, increased on exposure to ABA and reached maximum on the 4th day, This result shows that anthocyanin synthesis and PAL activity have a close physiological relationships. In the effects of temperatures ($10^{\circ}C,\;17^{\circ}C,\;25^{\circ}C$and $30^{\circ}C$) on anthocyanin accumulation and PAL activity in seedlings, a moderate-low temperatures ($17^{\circ}C$) enhanced both anthocyanin content and PAL activity, whereas elevated temperatures ($30^{\circ}C$) showed low levels of anthocyanin and PAL activity, suggesting a correlation between temperature-induced anthocyanin synthesis and the accumulation of PAL mRNA. Simultaneous application of ABA with temperatures Induced higher anthocyanin synthesis and PAL activity in seedlings than ABA or temperature stress alone. Moderate-low temperature with ABA exposure elicited the maximal induction of anthocyanin synthesis and PAL activity. Therefore, ABA treatment significantly increased thermotolerance in .A. thalinan seedlings. Ethephon and ABA showed similar mode of action in physiological effects on anthocyanin accumulation and PAL activity. Our data support that anthocyanins may be protective in preventing damage caused by environmental stresses and play an important role in the acquisition of freezing tolerance.

The effect of temperature and light on anthocyanin synthesis in Glehnia littoralis Fr. Schm. (갯방풍의 Anthocyanin생성에 미치는 온도 및 광의 영향)

  • 부희옥
    • Korean Journal of Plant Resources
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    • v.8 no.2
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    • pp.135-141
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    • 1995
  • The experiment was carried out to clarify the effects of air temperature, light exposure and UV light on the growth and anthocyanin syntheses of Glehnia littoralis Fr. Schm. The major pigment of purfied aglycone of Glehnia littoralis Fr. Schm. was identified as cyanidin by TLC and HPLC analysis. The anthocyanin Syntheses was promoted at low temperature and in the treatment of light exposure (12hrs a day) for 4-6 days after shading. The plants grown under UV-cut film had less anthocyanin content those tose under UV-transmission film.

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