• Title, Summary, Keyword: antibiotics susceptibility

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Results of Secondary Antibiotics Susceptibility Test for Gram Negative Bacilli Resistant to Primary Susceptibility Test in Yeungnam University Hospital (기본 항생제에 내성을 보인 그람 음성 간균에 대한 2차적 항생제 감수성 검사 결과)

  • Kim, Chung-Sook;Kim, Kyung-Dong;Lee, Chae-Hoon
    • Yeungnam University Journal of Medicine
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    • v.10 no.1
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    • pp.28-36
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    • 1993
  • Major pathogenic Gram-negative organisms such as P. aeruginosa, Serratia species, E. coli, Enterobacter species which are isolated from the specimens in large medical centers are greatly resistant to the commonly used antibiotics. Gram-negative bacilli, which had been isolated in Yeungnam University Hospital during the period from December 1992 to April 1993 and turned out to be resistant to the primary antibiotics susceptibility test for chloramphenicol, ampicillin, cephalothin, gentamicin, tetracyclin, amikin and tobramycin, were subjected to the secondary antibiotics susceptibility test for aztreonam, ceftazidime, ciprofioxacine, cefotaxime, cefamandole, piperacillin, ticarcillin and sulfamethoxazole trimethopime. Out of 315 tested organisms, 167 organisms(53%) were resistant to all secondary antibiotics in vitro. Antimicrobial activity of ceftazidime(37.1%), aztreonam(11.%), ciprofioxacine(7.9%) against Gram negative bacilli were slightly more active than other antibiotics tested, while cefamandole was not active to all the Gram-negative bacilli tested. According to the specimens, E. coli was the most frequently resistant organisms to the primary antibiotics from urine, A. baumanii, from respiratory system and wounds, and P. aeruginosa from various specimens. In summary, Gram negative bacilli resistant to the primarily applied antibiotics also were resistant to the secondary antibiotics. Rearrangement of the antibiotics disks for the antibiotic susceptibility test should be considered.

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ANTIBIOTIC SUSCEPTIBILITY IN MUTANS STREPTOCOCCI AND STREPTOCOCCUS ANGINOSUS ISOLATED FROM DENTAL PLAQUE (치면세균막에서 분리한 뮤탄스 연쇄상구균 및 Streptococcus anginosus의 수종 항생제에 대한 감수성 조사)

  • Kook, Joong-Ki;Lim, Sang-Soo;Yoo, So-Young;Hwang, Ho-Keel
    • Restorative Dentistry and Endodontics
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    • v.29 no.5
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    • pp.462-469
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    • 2004
  • The aim of this study was to investigate the susceptibility of mutans streptococci (S. mutans and S. sobrinus) and Streptococcus anginosus, for seven antibiotics, penicillin G, amoxicillin, ciprofloxacin, cefuroxime, erythromycin, bacitracin, and vancomycin. The minimum inhibitory concentration (MIC) of seven antibiotics against 3 species (type strains) of mutans streptococci and S. anginosus, 10 strains (wild type) of S. mutans, 7 strains (wild type) of S. sobrinus, and 11 strains (wild type) of S. anginosus, were measured by broth dilution method. All of the type strains of mutans streptococci and S. anginosus had the same susceptibility for penicillin G, amoxicillin, cefuroxime and bacitracin. Type strain of S. anginosus was sensitive in ciprofloxacin, but those of mutans streptococci were not. All of the clinical isolates of mutans streptococci and S. anginosus had the same susceptibility for the seven antibiotics. Our data reveal that mutans streptococci and S. anginosus have similar antibiotic-resistant character. In addition. these results may offer the basic data to verify the antibiotic-resistant mechanism of mutans streptococci and S. anginosus.

Species identification and antibiotics susceptibility of Staphylococci isolated from bovine mastitic milk and several animals in Kyungbuk province (경북지역 젖소 유방염 우유 및 각종 동물로부터 분리한 포도구균의 동정 및 항생제 감수성)

  • Kim, Sin;Kim, Soon-Tae;Kim, Woo-Hyun;Gwon, Heon-Il
    • Korean Journal of Veterinary Service
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    • v.21 no.3
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    • pp.301-311
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    • 1998
  • This study was carried out species identification and antibiotics susceptibility of Staphylococci Isolated from bovine mastitic milk, chicks, Korean indigenous goats, pigs and mice in northern area of Kyungbuk. The result were summarized as follows ; A total of 71 Staphylococci were Isolated from bovine mastitic milk, chicken, pig, Korean indigenous goat and mouse. The results of identification of 71 Staphylococci revealed that S. aureus was most important pathogen in animals tested. Of 39 Staphylococci from bovine mastitic milk, 16 of 39 isolates (41%) were S. aureus and 9 of 39 isolates (23% ) were S. hyicus subsp chromogens. The results of susceptibility test to 16 antibiotics revealed that 91.5% of all isolates were resistant to more than 1 antibiotic and resistance to penicillin was most high (76.1%), All Isolates were susceptible to vancomycin.

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Isolation of Enterobacteriaceae from bile, urine and intestine in slaughtered pigs and its susceptibility to antibiotics (도축돈의 담즙, 뇨 및 장관에서 장내세균의 분리 및 항생물질 감수성)

  • 허부홍;서석열;엄성심;김진환;윤창용;조정곤;송희종
    • Korean Journal of Veterinary Service
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    • v.19 no.3
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    • pp.213-219
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    • 1996
  • Seventy-nine strains of Enterobacteriaceae isolated from 117 slaughtered pigs (bile, urine, small intestine, cecum and rectum) in 1995 were examined for biotypes and susceptibility to 19 antibiotics with MicroScan WalkAway 40/96. The obtained results were as follows : 1. Among the twenty-two species isolated from the samples, Proteus mirabilis, E. coli and Enterobacter cloacae were commonly encountered. 2. The distribution frequency of isolates from cecum, small intestine, rectum, bile, and urine was 31(38.8%), 25(31.3%), 18(22.8%), 3(3.7% ), and 2(2.5% ), respectively. 3. A majority of isolates were sensitive to 16 antibiotics, singly or in combination. And these isolates were commonly susceptible to various antibiotics such as Cp, Ts, Azt, Caz, To, Gm, Cfz, Crm, Am and Cfx, in order. Whereas the Salmonella spp was susceptible to Cf, Ti and Pi, and Proteus mirabilis to Imp, Tim, Cft and Cz. Meanwhile, no effect was found to Cf, Ak and Cax. 4. Among the antibiotic resistant strains, a total of 17 reistant patterns was noted End of these Ak Tim 45(57.0%), Ak Am Cf Cfx Cfz Tim 8(10.1%) and Ak Ti Tim 6(7.6% ) were frequently encountered.

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Characteristics and Antibiotics Susceptibility of Escherichia coli Isolated from Fishery Products (수산물에서 분리한 대장균의 분리특성 및 항생제 감수성)

  • 이재인;한기영;박홍현
    • The Korean Journal of Food And Nutrition
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    • v.16 no.2
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    • pp.111-115
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    • 2003
  • This study was practiced to investigate characteristics and antibiotics susceptibility to 296 isolates of Escherichia coli isolated from 2429 fishery samples sold in Noryangjin Fishery Market from Sep. 2001 to Aug. 2002 It showed 12.2% average E. coli isolation rates for the tested samples. Isolation rates were high in summer, especially July and August, but there was no isolate in winter season. Of the fishery groups, the isolation rate showed in crustacea(15.6%), in mollusca(14.4%), in shellfish(12.0%) and in fish(11.0%). But, differences between the fishery groups was not large. In the case of shellfish, the isolation rate of shucked shellfish (16.3%) was higher than the non-shucked shellfish (6.8%). Following the provinces of origin of the fishery samples, in Gyung-Nam (14.3%) showed the highest isolation rate, whereas Je-Ju (6.9%) showed the lowest isolation rate. Results in 12 kinds of antibiotics susceptibility test, the 169 isolates(57.1%) had antibiotics resistances at least one kind of antibiotics and the 104 isolates (35.1%) had the multiple resistance at least two kinds.

Antibiotics susceptibility and biochemical characterization of Escherichia coli associated with diarrhea from preweaning piglets (포유자돈 소장에서 분리된 대장균의 생화학 성상과 항생제 감수성 결과)

  • Ham, Hee-jin;Min, Kyoung-sub;Chae, Chan-hee
    • Korean Journal of Veterinary Research
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    • v.37 no.4
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    • pp.773-777
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    • 1997
  • A retrospective study of 132 cases of natural Escherichia coli infection in preweaning piglets with diarrhea submitted to the Department of Veterinary Pathology in Seoul National University from 1995 to 1996 was performed to determine the biochemical characteristics and antibiotics susceptibility. Most field isolates were lysine decarboxylase-positive (99.2%), phenylalanine decarboxylase-negative (100%), and fermented sorbitol (91.7%). Antibiotic susceptibilities will be determined by agar diffusion method. A large percentage of isolates were resistant to many antibiotics in common usage. Most isolates were susceptible to colistin (98.8%), gentamycin (64.3%), amikacin (100%), and ceftiour sodium (64.2%), whereas most isolates were notably resistant to ampicillin (86.9%), neomycin (66.7%), streptomycin (84.5%), tetracycline (98.8%), penicillin (98.8%), and amoxacillin (58.3%).

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Distribution and Antibiotic Susceptibility Patterns of Genus Mycobacterium at a Private Hospital, Korea

  • Hong, Sung Kyun;Hur, Sung-Ho;Seong, Hee-Kyung
    • Biomedical Science Letters
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    • v.19 no.2
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    • pp.132-141
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    • 2013
  • Mycobacterium isolates were retrospectively identified, antibiotics susceptibility test results and basic clinical data were analyzed for the 715, excepted 308 in 1,023 specimens, from a mycobacterial laboratory at a tertiary care hospital from September 2002 to December 2008. Their male to female ratio was 1.12 to 1 (379 male, 336 female). The median age of study population was 47 years (range from 10 to 93 years). Distribution of Mycobacterium species was 90.1% of total were isolates Mycobacterium tuberculosis, and 9.9% of the total non-tuberculosis Mycobacterium isolated, and Among nontuberculosis Mycobacterium isolates, 60.6% were Mycobacterium avium complex, 14.1% were isolates Mycobacterium abscessus, and 12.7% were isolates Mycobacterium intracellulare. Among 526 Mycobacterium tuberculosis isolates, 81.7% isolates were susceptible to first line antibiotics, 18.3% were resistant to one or more antibiotics. Non-tuberculosis Mycobacterium isolates, all were resistant to two or more antibiotics. Multi-antibiotic resistant tuberculosis rate was show 10.2% of total specimens. Isolated Mycobacterium species, 19.2% were multi-antibiotic resistant tuberculosis, and the rate of nontuberculosis Mycobacterium resistant to isoniazid and rifampin was very highly 84.5%. Thus among acid fast bacilli culture positive cases, Mycobacterium tuberculosis and non-tuberculosis Mycobacterium were must exactly identification and antibiotic sensitivity test. It was considered to help to select of the antibiotic in preventive medicine.

Selection of Clinically Isolated Strains for Evaluation of the Newly Synthesized Antibiotics (새로운 $\beta$-lactam계 항생물질 개발을 위한 검정용 균주의 개발)

  • 김대진;최금화;김숙경;최성숙;김병각;강창율;최응칠
    • YAKHAK HOEJI
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    • v.39 no.2
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    • pp.131-136
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    • 1995
  • Clinically isolated bacterial strains resistant to almost of all the clinically superior .betha.-lactam antibiotics can be used to screen the promising ones among the newly synthesized $\beta$-lactam antibiotics. To select the resistant strains, the susceptibility of 389 strains of S. aureus, 144 strains of coagulase negative staphylococci, 509 strains of E. coli, 115 strains of E. cloacae and 187 strains of P. aeruginosa to methicillin, ampicillin, piperacillin and gentamicin was determined. The susceptibility of 19 bacterial strains selected through the first screening to cefixime, cefotiam, cefotaxime, flomoxef, cepfirome, cefdnir, SCE-2787, panipenem and imipenem was determined. Four strains of S. aureus finally selected have high degree of resistance to almost of all $\beta$-lactam antibiotics used and also produce $\beta$-lactamases. These 4 strains of S. aurues can be used to screen effectively the promising $\beta$-lactam antibiotics among the numerous numbers of the newly synthesized $\beta$-lactam antibiotics.

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Comparison of biological and chemical assays for measuring the concentration of residual antibiotics after treatment with gamma irradiation

  • Nam, Ji-Hyun;Shin, Ji-Hye;Kim, Tae-Hun;Yu, Seungho;Lee, Dong-Hun
    • Environmental Engineering Research
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    • v.25 no.4
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    • pp.614-621
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    • 2020
  • Antibiotic pollution is one of the factors contributing to the spread of antibiotic-resistant bacteria in the environment. Advanced oxidation and irradiation processes have been introduced to eliminate antibiotics from water and wastewater. However, few studies have reported the toxic effects of residual antibiotics and their byproducts induced by a treatment system. In this study, we compared the efficacies of chemical (high-performance liquid chromatography (HPLC)) and biological (antimicrobial susceptibility test) assays for measuring the concentrations of residual antibiotics after gamma irradiation for degrading amoxicillin, cephradine, lincomycin, and tetracycline. The concentrations of residual antibiotics estimated using the two assay methods were almost identical, except cephradine. In the case of cephradine, inhibited bacterial growth was observed that was equivalent to twice the concentration measured by HPLC in the samples subjected to gamma irradiation. The observed inhibition of bacterial growth suggested the generation of potentially toxic intermediates following antibiotic degradation. These results indicate that biological and chemical assays should be used in concert for monitoring antibiotic contamination and the toxic derivatives of antibiotic degradation. The results demonstrate that these four antibiotics can be decomposed by 2.0 kGy gamma-irradiation without toxic effects of their byproducts.