• Title, Summary, Keyword: asparagine

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Study of Asparagine Extraction and analysis (Asparagine 추출 및 분석에 관한 연구)

  • 이윤배;김원길
    • Proceedings of the KAIS Fall Conference
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    • pp.314-316
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    • 2003
  • 체내 알코올을 분해하는데 효과적인 물질인 Asparagine은 자연물(예: 콩나물)에서도 추출이 가능하며 이를 이용하여 실제 체내의 알코올 분해작용이 가능하다. 콩나물에는 특히 뿌리부분에 더욱 많은 Asparagine이 추출됨을 알 수 있으며, 실제로 알코올 제품에 함유되어 있는 소주 내의 Asparagine의 량을 확인해본다. 또한 여러 제품에도 Asparagine의 함유량이 다름을 살펴보았다.

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Effects of Soy-sprout Asparagine on Hangover (콩나물의 Asparagine이 숙취에 미치는 영향)

  • Jeong, Yeon-Shin;Dhakal, Krishna Hari;Hwang, Young-Hyun
    • Current Research on Agriculture and Life Sciences
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    • v.27
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    • pp.53-58
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    • 2009
  • This experiment was conducted to determine the amount of asparagine content in soybean sprout soup as well as its effect on the regulation of blood alcohol concentration in human body. Asparagine content and alcohol concentration in blood were analyzed using the amino-acid analyzer and alcohol tester, respectively. Asparagine content in soybean sprout soup was highly significantly different among sprout soups sampled from different restaurants, although the boiling time and amount of sprout used were different among the samples. Significantly higher asparagine content was observed in soups with higher amounts of sprouts (2.81% in 500 grams of sprout) and 10 minutes after boiling with other solid ingredients in the soup. Lower concentration of alcohol in blood was recorded in persons with higher body weight. The asparagine+soybean extract (eaten immediately after drink) lowered the blood alcohol concentration significantly followed by only asparagine and control. The blood alcohol concentration after drinking become zero earlier (by 30 minutes), when asparagine+soybean extract or only asparagine was consumed as compared to control.

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Change of asparagine content in soybean sprouts by variety, root growth, and cultivation period (콩나물의 품종, 뿌리발생 및 재배일수에 따른 asparagine 함량 변이)

  • Jeong, Yeon-Shin;Park, Hee Joon;Dhakal, Krishna Hari;Lee, Jeong-Dong;Lee, In-Jung;Hwang, Young-Hyun
    • Current Research on Agriculture and Life Sciences
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    • v.26
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    • pp.63-69
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    • 2008
  • The varietal difference, effect of root amounts and cultivation duration on the asparagine content of soybean sprouts, which is known to have the excellent detoxifying effect of ethanol, were investigated for developed varieties and indigenous sprout lines to establish cultivation methods of increasing the asparagine content and to develop soybean varieties having high asparagine content. Some of the research results obtained are summarized as follows; 1. Range and mean of asparagine content of 174 germplasm were 0.38~1.67% and 0.99%, respectively, on fresh weight basis. 2. Developed sprout-soybean varieties showed somewhat higher asparagine content of 1.29% than that of indigenous sprout lines of 0.96% on fresh weight basis. 3. No significant difference in asparagine content among the seed size groups was recognized though the highest asparagine content, 1.02% on fresh weight basis was observed in the seed size of 8.1~10.0g/100 seeds. 4. Among the seed coat color groups, soybean of brown seed coat color showed the highest asparagine content (1.15%) on fresh weight basis. No difference was observed among other groups of seed coat color. 5. Range of asparagine content of 174 varieties was 4.08~6.24% on dry weight basis. Soybean varieties that showed high asparagine content were Dawonkong, Sobacknamulkong, Sowonkong, and Somyungkong, with the contents of 6.24%, 6.21%, 5.95%, and 5.85%, respectively. 6. Amount of roots which have the highest asparagine content out of sprout parts was greatly increased up to 10 days of sprout cultivation. 7. Highly significant difference in asparagne content of sprout was recognized between those grown for five and ten days, with the asparagine content of 0.68% and 1.21%, respectively, on fresh weight basis. 8. Asparagine content of hypocotyle part was also highly proportional to days to cultivation; it increased from 2.91% at 2 days of cultivation to 15.68% on fresh weight basis at 14 days of cultivation.

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Change of asparagine content during soy-sprout growing (콩나물 재배시 asparagine의 변화)

  • Jeong, Yeon-Shin;Dhakal, Krishna Hari;Hwang, Young-Hyun
    • Current Research on Agriculture and Life Sciences
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    • v.27
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    • pp.43-52
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    • 2009
  • Most soybean sprouts grown in factories are sold on the market. When the soybean sprouts grow up to lateral root initiation, the rate and absolute amount of asparagine in the roots of all sprouts are comparatively low. To extract the greatest amount of asparagine from soy-sprouts, it is advantageous to grow sprouts more than 16 days. When sprouts were grown with ozonic water, it was possible to grow sprouts more than 16 days without any rotting problems. The content of asparagine in the sprouts were proportionally increased up to 16 days. When sprouts were grown in 0.2% of urea, the content of asparagine in sprouts were significantly increased. Soybean sprouts grown at $20^{\circ}C$ room temperature showed the highest yield rate and asparagine content, at the same time.

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Variation of Asparagine and Aspartic Acid Contents in Beansprout Soybeans (나물콩 품종의 Asparagine과 Aspartic acid 함량 변이)

  • 이준찬;황영현
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.5
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    • pp.592-599
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    • 1996
  • The variations of asparagine and aspartic acid contents among the soybean varieties and in the different parts of soybean sprout after the different durations of storage were investigated. Asparagine and aspartic acid are known to have the detoxifying effects on acetaldehyde, which is highly toxic metabolites in the process of alcohol metabolism in the human body. The contents of asparagine and aspartic acid of beansprout showed continuosuly increasing trends along with the days to cultivation with a great varietal difference, especially in the roots. The duration of seed storage did not affect contents of asparagine content; the longer storage decreased aspartic acid content remarkably.

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Glucose Binging Affinity of DPPC-ODA-asparagine and Stability of Liposomes Adding Cholesterol (DPPC-ODA-asparagine 리포솜의 포도당 친화도 및 콜레스테롤 첨가에 따른 안정성 측정)

  • 문제영;이기영;김진철;박기남
    • KSBB Journal
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    • v.16 no.2
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    • pp.170-173
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    • 2001
  • Liposome-amino acid conjugates were prepared using dipalmitolyphosphatidylcholine(DPPC) and hydrophobically modified asparagine. A microdialyzer was used to measure glucose diffusion. The glucose binding affinity of DPPC-ODA-asparagine liposomes higher than that of DPPC liposomes and distilled water. The size of DPPC-ODA-asparagine was approximately 75-150 nm. Cholesterol increased the stability of liposomes, and reduced the size of liposome particles.

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Changes in the Compositions of Amino Acids in the Rice Seedlings under Low Temperature (저온처리한 벼 유식물의 아미노산 조성의 변화)

  • 문병용
    • Journal of Plant Biology
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    • v.32 no.4
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    • pp.235-245
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    • 1989
  • The contents and the compositions of total free amino acids were investigated in the rice(Oryza sativa L. cv. Chuncheong) seedlings under low temperatures. Activities of some enzymes associated with the markedly changed amino acid content were also investigaetd. Under low temperature, the contents of soluble protein and the total free amino acids increased, while the content of total nitrogen decreased. Although asparagine+glycine were the most abundant amino acid speceis in the rice seedlings at the control temeprature, low temperature treatment for 3days brought about the decrease in their amount to about 60% level of the control plants. On the other hand, alanine showed the highest increase in the content among all the free amino acids, though glutamine, proline, asprtic acid, valine and tyrosine also increased after low temperature treatment. To eludicate the decrease of asparagine+glycine level under low temperature, the activities of asparagine aminotransferase and asparaginase which metabolize asparagine were investigated in the rice seedlings under low temperature. The activity of asparaginase increased markedly, while that of asparagine aminotransferase decreased under low temperatures. Therefore, it was suggested that asparaginase metabolizes asparagine predominatly in the rice seedlings under low temperatures.

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Determination of L-Asparagine Using Proteus mirabilis Bacterial Electrode (Proteus mirabilis 박테리아 전극을 이용한 L-Asparagine의 정량)

  • Ihn Gwon-Shik;Moo-Jeong Sohn
    • Journal of the Korean Chemical Society
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    • v.32 no.5
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    • pp.422-427
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    • 1988
  • The bio-electrode for L-asparagine, excellent in the reproducibility of responsibility, has been constructed by immobilizing the bacterium Proteus mirabilis on an ammonia gas sensor. This electrode was investigated for the effects of pH, temperature, buffer solution, bacterial amounts and interferences, and stability with the lapse of time. The response of the bacterial electrode was linear in the range of $9.0{\times}10^{-5}$$1.0{\times}10^{-2}M$ L-asparagine with a slope of 58.9mV/decade in pH 7.8, 0.05M phosphate buffer solution at $30^{\circ}C$. The bacterial amounts used for this electrode was 3 mg and response time was 7∼9 min. Therefore, this assembly can be used for the determination of L-asparagine.

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Studies on the Nitrogen Metabolism of Soybeans -III. Variation of Glutamic acid, Aspartic acid and its Amides during the Growth of Yonger Plants (대두(大豆)의 질소대사(窒素代謝)에 관(關)한 연구(硏究) -III. 유식물(幼植物) 시기(時期)에서의 Glutamine 산(酸)과 Asparagine 산(酸) 및 그 Amide의 소장(消長))

  • Kang, Y.H.
    • Korean Journal of Soil Science and Fertilizer
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    • v.3 no.1
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    • pp.55-59
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    • 1970
  • In an effort to determine the bio-synthesis in the soybean as investigate to the variance of each substance: Glutamic acid, Aspartic acid and its amides during the growth of younger soybean plants. 1. The variance-curve of Gultamic acid and Aspartic acid as the acidic amino acids in the cotyledons was appeared the peak the first half period at Glutamic acid and the latter half at Aspartic acid in the growth of soybeans, and was received the symmetrical impression centering around the stage of adult leaf-development. But, in the embryonic organ, it appears the peak at both part, in the developmental stage of adult leaf and also appears near phenomena of increase and decrease in the variation-curve of metabolites. 2. It's amides-Gultamine and Asparagine-appears the peak at the developmental stage of adult leaf in the both cotyledons and embryonic organ, and rapid increase in the cotyledons were very impressed compare with the decrease at fallen stage of cotyledons in the embryonic organs. 3. In the relation of variance at Glutamic acid and Aspartic acid, both substance were discovered the fact of translocation from cotyledon to embryonic organ, and Glutamic acid could supposed that bear the charges of outrider substance in other amino acid as the Glutamic acid-self and major basic function for receiving the ammonia as the nitrogen contain constituent of plant. In the case of Glutamine, formation-mechanism of ammonia which develops due to its hydrolysis in the latter period of soybean growth, suggested that was forfeit its function till instance of fallen cotyledons. 4. In the relation the Aspartie acid and Asparagine, Aspartic acid which begins to decrease from seed-state was supposed that bear sufficiently the charge of outrider substance in the formation of Asparagine other than translocated to embryonic organ from cotyledon. And, formation-theory of Aspartic acid which suppose as formational substance from Kreb's cycle were recognized from latter period of soybean growth, and then, rapid accumulation of Asparagine's amounts were supposed that adapt to two theory: Theory which consider to transformation as Asparagine state for pressing to less than noxious weight the concentration of ammonia developing from the cells, and was formate and accumulate as ammonia or carbohydrates containing excess in the cotyledons.

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