• Title, Summary, Keyword: bacterial wilt

Search Result 153, Processing Time 0.054 seconds

Studies on the Inheritance of Resistance to Bacterial Wilt(Ralstonia solanacearum) in Tobacco(Nicotiana tabacum L.) (연초의 세균성마름병 저항성 유전에 관한 연구)

  • 정석훈
    • Journal of the Korean Society of Tobacco Science
    • /
    • v.22 no.1
    • /
    • pp.25-30
    • /
    • 2000
  • Bacterial wilt(Ralstonia solanacearum) is one of the major diseases of flue-cured tobacco (Nicotiana tabacum L.) in the world. This study was conducted to investigate degree of dominance, selection, and correlation between leaf shape and degree of bacterial wilt resistance in flue-cured tobacco. The degree of disease caused by bacterial wilt was evaluated in parents, F$_1$, F$_2$ and F$_3$ populations of two crosses, BY 4 x NC 95 and BY 4 x Coker 86, in the infected field. The leaf shape index was also measured in parents and F$_2$ population of BY 4 x NC 95. The incidence of bacterial wilt was observed in the middle of June and peaked in late July, when the highest value of pathogen density reached 1.0 x 10$^{6}$ colony forming unit per gram. It was concluded that the inheritance mode of risestance to bacterial wilt in the above two crosses of susceptible and resistant varieties was recessive and polygenic. The resistance to bacterial wilt was significantly correlated with leaf shape in F2 generation of BY 4 x NC 95. But certain plants having narrower leaves were also resistant to bacterial wilt. It is considered that the bacterial wilt resistant lines having narrower leaves could be selected. The selection for bacterial wilt resistance in the F$_2$ population might be effective.

  • PDF

Suppression of Bacterial Wilt with Fuorescent Pseudomonads, TS3-7 strain (Fluorescent siderophore 생산균주, TS3-7에 의한 풋마름병 발병 억제)

  • Kim, Ji-Tae;Cho, Hong-Bum;Kim, Shin-Duk
    • Applied Biological Chemistry
    • /
    • v.48 no.3
    • /
    • pp.296-300
    • /
    • 2005
  • Among the root colonizing and plant growth promoting bacteria isolated from the bacterial wilt suppressive soil, five strains were detected to produce siderophores by CAS agar assay. The most effective isolate, TS3-7 strain induced significant suppression of bacterial wilt disease in tomato and pepper plants. Seed treatment followed by soil drench application with this strain resulted in over 80% reduction of bacterial wilt disease compared with the control. Significant disease suppression by TS3-7 strain was related to the production of siderophore. Besides iron competition, induction of resistance of the host plant with siderophore was suggested to be another mode of action that suppress bacterial wilt, based on the lack of direct antibiosis against pathogen in vitro. According to Bergey's Manual of Systemic Bacteriology and 16S rDNA sequence data, TS3-7 stain was identified as Pseudomonas sp. TS3-7.

Control Strategy of Acidified Nutrient Solution on Bacterial Wilt of Tomato Plants (산성배양에 공급에 의한 토마토 풋마름병 방제)

  • 이영근;설균찬
    • Korean Journal Plant Pathology
    • /
    • v.14 no.6
    • /
    • pp.744-746
    • /
    • 1998
  • Control effect of acidified nutrient solution on bacterial wilt of tomato plants was tested by examining the degree of bacterial growth inhibition and plant damage due to the acidity. Ralstonia solanacearum, the causal bacterium of bacterial wilt of tomato plants, showed 105 times population reduction when the bacterium was cultured in the acidified nutrient solution (pH 3.5∼4.0). However, fruit yields were decreased only fifteen to twenty percents. These results suggest that control of the bacterial wilt of tomato plants may be possible with supplying acidified nutrient solution.

  • PDF

Biocontrol Potential of a Lytic Bacteriophage PE204 against Bacterial Wilt of Tomato

  • Bae, Ju Young;Wu, Jing;Lee, Hyoung Ju;Jo, Eun Jeong;Murugaiyan, Senthilkumar;Chung, Eunsook;Lee, Seon-Woo
    • Journal of Microbiology and Biotechnology
    • /
    • v.22 no.12
    • /
    • pp.1613-1620
    • /
    • 2012
  • Bacterial wilt caused by Ralstonia solanacearum is a devastating disease of many economically important crops. Since there is no promising control strategy for bacterial wilt, phage therapy could be adopted using virulent phages. We used phage PE204 as a model lytic bacteriophage to investigate its biocontrol potential for bacterial wilt on tomato plants. The phage PE204 has a short-tailed icosahedral structure and double-stranded DNA genome similar to that of the members of Podoviridae. PE204 is stable under a wide range of temperature and pH, and is also stable in the presence of the surfactant Silwet L-77. An artificial soil microcosm (ASM) to study phage stability in soil was adopted to investigate phage viability under a controlled system. Whereas phage showed less stability under elevated temperature in the ASM, the presence of host bacteria helped to maintain a stable phage population. Simultaneous treatment of phage PE204 at $10^8$ PFU/ml with R. solanacearum on tomato rhizosphere completely inhibited bacterial wilt occurrence, and amendment of Silwet L-77 at 0.1% to the phage suspension did not impair the disease control activity of PE204. The biocontrol activities of phage PE204 application onto tomato rhizosphere before or after R. solanacearum inoculation were also investigated. Whereas pretreatment with the phage was not effective in the control of bacterial wilt, post-treatment of PE204 delayed bacterial wilt development. Our results suggested that appropriate application of lytic phages to the plant root system with a surfactant such as Silwet L-77 could be used to control the bacterial wilt of crops.

Plant-derived Antibacterial Metabolites Suppressing Tomato Bacterial Wilt Caused by Ralstonia solanacearum

  • Vu, Thuy Thu;Choi, Gyung Ja;Kim, Jin-Cheol
    • Research in Plant Disease
    • /
    • v.23 no.2
    • /
    • pp.89-98
    • /
    • 2017
  • Ralstonia solanacearum species complex (RSSC) causes bacterial wilt, and it is one of the most important soil-borne plant pathogenic bacteria. RSSC has a large host range of more than 50 botanical families, which represent more than 200 plant species, including tomato. It is difficult to control bacterial wilt due to following reasons: the bacterial wilt pathogen can grow inside the plant tissue, and it can also survive in soil for a long period; moreover, it has a wide host range and biological diversity. In most previous studies, scientists have focused on developing biological control agents, such as antagonistic microorganisms and botanical materials. However, biocontrol attempts are not successful. Plant-derived metabolites and extracts have been promising candidates to environmentally friendly control bacterial wilt diseases. Therefore, we review the plant extracts, essential oils, and secondary metabolites that show potent in vivo antibacterial activities (in potted plants or in field) against tomato bacterial wilt, which is caused by RSSC.

Biological Control of Soilborne Diseases on Tomato, Potato and Black Pepper by Selected PGPR in the Greenhouse and Field in Vietnam

  • Thanh, D.T.;Tarn, L.T.T.;Hanh, N.T.;Tuyen, N.H.;Srinivasan, Bharathkumar;Lee, Sang-Yeob;Park, Kyung-Seok
    • The Plant Pathology Journal
    • /
    • v.25 no.3
    • /
    • pp.263-269
    • /
    • 2009
  • Bacterial wilt, Fusarium wilt and Foot rot caused by Ralstonia solanacearum, Fusarium oxysporum, and Phytophthora capsici respectively, continue to be severe problems to tomato, potato and black pepper growers in Vietnam. Three bio-products, Bacillus vallismortis EXTN-1 (EXTN-1), Bacillus sp. and Paenibacillus sp. (ESSC) and Bacillus substilis (MFMF) were examined in greenhouse bioassay for the ability to reduce bacterial wilt, fusarium wilt and foot rot disease severity. While these bio-products significantly reduced disease severities, EXTN-1 was the most effective, providing a mean level of disease reduction 80.0 to 90.0% against bacterial wilt, fusarium wilt and foot rot diseases under greenhouse conditions. ESSC and MFMF also significantly reduced fusarium wilt, bacterial wilt and foot rot severity under greenhouse conditions. Bio-product, EXTN-1 with the greatest efficacy under greenhouse condition was tested for the ability to reduce bacterial wilt, fusarium wilt and foot rot under field condition at Song Phuong and Thuong Tin locations in Ha Tay province, Vietnam. Under field condition, EXTN-1 provided a mean level of disease reduction more than 45.0% against all three diseases compared to water treated control. Besides, EXTN-1 treatment increased the yield in tomato fruits 17.3% than water treated control plants.

Resistance to Bacterial Wilt in Pepper (Capsicum anuum L.) (고추의 풋마름병(靑枯病)에 대한 저항성)

  • 임양숙;김병수
    • Korean Journal Plant Pathology
    • /
    • v.10 no.2
    • /
    • pp.73-77
    • /
    • 1994
  • Experiments were conducted to determine the method of nioculation and inoculum level, and the optimum age of pepper seedlings for evaluation of resistance to bacterial wilt in pepper. Injection of bacterial suspension(107~108 cells/ml) to the leaf axil of the 3rd or 4th leaf of pepper, and drenching the soil planted with pepper seedling after wounding roots with scalpel, resulted in good varietal difference in resistance to bacterial wilt. PI377688, PI358812 and PI369994 of 298 open-pollinated lines and 10 hybrids tested for resistance to bacterial wilt were highly resistant and such local cultivars as Masan, Anjinbaengi, Kimyongcho and Punggakcho moderately resistant.

  • PDF

First Report of Bacterial Wilt Caused by Ralstonia solanacearum Biovar 2 Race 1 on Tomato in Egypt

  • Seleim, Mohamed A.A.;Abo-Elyousr, Kamal A.M.;Abd-El-Moneem, Kenawy M.;Saead, Farag A.
    • The Plant Pathology Journal
    • /
    • v.30 no.3
    • /
    • pp.299-303
    • /
    • 2014
  • This study aims to isolate and identify the causal pathogen of tomato bacterial wilt in Egypt. In 2008, tomato plants showing typical symptoms of bacterial wilt disease with no foliar yellowing were observed in Minia, Assiut and Sohag governorates, Egypt. When cut stems of symptomatic plants were submerged in water, whitish ooze was evident and longitudinal sections showed a brown discoloration in the vascular tissues. Bacteria were isolated on triphenyl tetrazolium chloride medium and fifteen isolates shown typical morphological and cultural characteristics were confirmed as Ralstonia solanacearum biovar 2 race 1. Pathogenicity tests showed that all isolates proved to be pathogenic to tomato plants, varied from 52 to 97% wilting. This is the first report of R. solanacearum biovar 2 race 1 causing bacterial wilt in tomato crop in Egypt.

Screening Procedure of Tobacco Cultivars for Resistant to Bacterial Wilt Caused by Ralstonia solanacearum (담배세균성마름병[립고병(立枯病)]에 대한 담배품종의 저항성 검정법)

  • Jeon, Yong-Ho;Kang, Yue-Gyu
    • Journal of the Korean Society of Tobacco Science
    • /
    • v.30 no.1
    • /
    • pp.1-7
    • /
    • 2008
  • Bacterial wilt caused by Ralstonia solanacearum has become a severe problem on tobacco in Korea. No effective single control measure is available at present time. One of the most potential way for controlling the bacterial wilt on tobacco is growing tobacco cultivars resistant to the bacterial wilt. In this study, optimal conditions for screening tobacco cultivars resistant to the bacterial wilt were examined to provide reproducible and efficient methods in growth chamber testing and field experiments for evaluating plant disease resistance. For this, already-known inoculation methods, inoculum densities, and incubation temperature, and plant growth stages at the time of inoculation were compared using tobacco cultivars resistant (Nicotiana tabacum cv, NC95), moderately resistant (N. tabacum cv. SPG70), and susceptible (N. tabacum BY4) to the bacterial disease. It was determined that root-dipping of tobacco seedlings at six true leaf stage into the bacterial suspension with inoculum level of $10^8$ colony-forming units (CFU)/ml for 20 min before transplanting was simple and most efficient in testing for resistance to the bacterial wilt of tobacco caused by R. solanacearum, for which disease incidences and severities were examined at 2 weeks of plant growth after inoculation at $20{\sim}25^{\circ}C$ in a growth chamber. These experimental conditions could discriminate one tobacco cultivar from the others by disease severity better than any other experimental conditions. In field testing, the optimum time for examining the disease occurrence was late June through early July. These results can be applied to establishing a technical manual for the screening of resistant tobacco cultivars against the bacterial wilt caused by R. solanacearum.

Relationship between the Population of Ralstonia solanacearum in Soil and the Incidence of Bacterial Wilt in the Naturally Infested Tobacco Fields

  • Chung, Yun-Hwa;Yu, Yun-Hyun;Kang, Yue-Gyu
    • The Plant Pathology Journal
    • /
    • v.20 no.4
    • /
    • pp.289-292
    • /
    • 2004
  • The population of Ralstonia solanacearum (Rs) in soil is very important as a primary inoculum source of bacterial wilt in tobacco fields. To investigate the population of Rs, physical properties and chemical components during the tobacco growing season, soil samples were taken from the fifteen fields which were located in the flue-cured tobacco growing area, Ansung, Kyunggi province and Wonju, Kangwon province. Two fields of the fifteen were bacterial wilt free. Six fields had less than 10% plants being diseased and seven over 10%. The Rs population level determined by using SMSA medium generally showed an up-and-down pattern being low in May, high in Jun and July and low in August. The soil population in May and June showed a positive correlation with the incidence of bacterial wilt (r=0.571$^*$, r=0.688$^{**}$), but P$_2O_5$, content of soil was negatively correlated with the disease incidence (r=-0.539$^*$). These results suggest that Rs population in soil examined in May or in June, and the P$_2O_5$ content in soil should be key factors to determine the bacterial wilt potential of tobacco fields.