• Title, Summary, Keyword: biochemical properties

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Biochemical Properties and Antimicrobial Drug Susceptibility of Pasteurella haemolytica Isolated from Pneumonic Calves (호흡기질병 감염 송아지에서 분리한 Pasteurella haemolytica의 생화학적 특성 및 약제 감수성)

  • 조광현;박노찬;권헌일;김이준;박덕상
    • Korean Journal of Veterinary Service
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    • v.14 no.2
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    • pp.121-126
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    • 1991
  • The present study was conducted to investigate biochemical properties and antimicrobial drug susceptibilities of 36 strains of Pasteurella haemolytica(P haemolytica) isolated from pneumonic calves in Kyongbuk province during the period from January 1990 to December 1990. P haemolytica was isolated from 36 of 111(32.4%) pneumonic calves of 1 to 6 months of age. The majority of biochemical and cultural properties of P haemolytica isolated from calves were identical to those of the reference strains employed. All isolated were susceptible to baytril, gentamicin, and kanamycin, some of them were resistant to tetracycline, amikacin, streptomycin, and ampicillin.

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Biochemical properties and antimicrobial drug susceptibility of Erysipelothrix rhusiopathiae isolated from the cases of acute septicemic swine erysipelas (급성 폐혈증형 돼지단독예에서 분리한 Erysipelothrix rhusiopathiae의 생화학적 특성 및 약제감수성)

  • Baek, Young-sook;Cho, Gil-jae;Kim, Bong-hwan
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.413-420
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    • 1990
  • The present study was conducted to investigate biochemical properties and antimicrobial drug susceptibilities of 47 strains of E rhusiopathiae isolated from the cases of acute septicemic swine erysipelas in Youngnam and Kyunggi provinces during the period from June 1988 to December 1989. The isolants were identified as E rhusiopathiae on the basis of cellular and colonial morphology, and characteristic reactions in some biochemical tests. All the organisms produced hydrogen sulfide in triple sugar iron agar and showed the characteristic "pipe cleaner" type of growth in gelatin stab cultures. The majority of biochemical and cultural properties of E rhusiopathiae isolated from pigs affected with acute erysipelas were identical to those of the reference strains employed. All the isolates were highly susceptible to penicillin G, ampicillin, erythromycin (MIC:0.025~0.39IU or ${\mu}g/ml$), and moderately susceptible to oleandomycin, oxytetracycline, chloramphenicol(MIC:$0.78{\sim}25{\mu}g/ml$). Kanamycin and sulfadimethoxine showed no activity against the isolates(MIC:>$400{\mu}g/ml$). The MICs of dihydrostreptomycin presented two distribution peaks; of 47 strains, 5(10.6%) were resistant to dihydrostreptomycin (MIC:$400{\mu}g/ml$), while the majority of them were susceptible to the drug.

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Synthesis of Curcumin Glycosides with Enhanced Anticancer Properties Using One-Pot Multienzyme Glycosylation Technique

  • Gurung, Rit Bahadur;Gong, So Youn;Dhakal, Dipesh;Le, Tuoi Thi;Jung, Na Rae;Jung, Hye Jin;Oh, Tae Jin;Sohng, Jae Kyung
    • Journal of Microbiology and Biotechnology
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    • v.27 no.9
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    • pp.1639-1648
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    • 2017
  • Curcumin is a natural polyphenolic compound, widely acclaimed for its antioxidant, anti-inflammatory, antibacterial, and anticancerous properties. However, its use has been limited due to its low-aqueous solubility and poor bioavailability, rapid clearance, and low cellular uptake. In order to assess the effect of glycosylation on the pharmacological properties of curcumin, one-pot multienzyme (OPME) chemoenzymatic glycosylation reactions with UDP-${\alpha}-{\text\tiny{D}}$-glucose or UDP-${\alpha}-{\text\tiny{D}}$-2-deoxyglucose as donor substrate were employed. The result indicated significant conversion of curcumin to its glycosylated derivatives: curcumin 4'-O-${\beta}$-glucoside, curcumin 4',4"-di-O-${\beta}$-glucoside, curcumin 4'-O-${\beta}$-2-deoxyglucoside, and curcumin 4',4"-di-O-${\beta}$-2-deoxyglucoside. The products were characterized by ultra-fast performance liquid chromatography, high-resolution quadruple-time-of-flight electrospray ionization-mass spectrometry, and NMR analyses. All the products showed improved water solubility and comparable antibacterial activities. Additionally, the curcumin 4'-O-${\beta}$-glucoside and curcumin 4'-O-${\beta}$-2-deoxyglucoside showed enhanced anticancer activities compared with the parent aglycone and diglycoside derivatives. This result indicates that glycosylation can be an effective approach for enhancing the pharmaceutical properties of different natural products, such as curcumin.

Biochemical properties and cultural characteristics of Escherichia coli isolated from chickens (닭에서 분리한 Escherichia coli의 생물화학적 및 배양 특성)

  • Woo, Yong-ku;Kim, Ki-seuk;Kim, Bong-hwan
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.421-425
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    • 1990
  • The present study was conducted to investigate the biochemical and cultural characteristics of Escherichia coli isolates from clinically affected chickens during the period from May 1988 to June 1989. A total of 82 E coli cultures were isolated from lesions of 75 chickens with colisepticemia. Biochemical properties of E coli isolates tested were in accordance with the general classification standard; all the isolates showed positive reaction in Catalase, Indol, and Methyl-Red tests, but negative reaction in Oxidase, Urease, $V{\ddot{o}ges$-Proskauer, Citrate utility, $H_2S$, Phenylalanine diaminase, and malonate tests. And the carbohydrate fermentation rates of them were shown to be variable. of the 82 isolates, 48(58.5%) cultures produced colicin to inhibit the indicator strain of E coli.

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A Testing Method of Indoor Artificial Acceleration for the evaluation of Biochemical Corrosion Properties of Sewage Concrete (하수시설 콘크리트의 생화학적 부식특성 평가를 위한 실내 인공촉진 시험방법)

  • Lee, Eui-Bae;Kim, Do-Su;Khil, Bae-Su;Lee, Seung-Hoon;Kim, Gyu-Yong;Kim, Moo-Han
    • Proceedings of the Korea Concrete Institute Conference
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    • pp.69-72
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    • 2006
  • Recently sewage facilities mainly consisted of concrete structures are being deteriorated seriously by biodeterioration originated from sulfur-oxidizing bacteria. To prevent biochemical corrosion of the sewage concrete, antibiotics which prevent growth of sulfur-oxidizing bacteria were developed. Existing methods to evaluate properties of biochemical corrosion of concrete examine the antimicrobial performance and resistance to sulfuric acid separately, but don't complexly. So, in this study, new method to test properties of biochemical corrosion of concrete complexly is suggested.

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Biochemical properties and antimicrobial susceptibility of Pasteurella multocida isolated from pneumonic lungs in slaughtered animals (도축장 출하축의 폐병변에서 분리한 Pasteurella multocida의 생화학적 특성 및 약제감수성)

  • 김성국;이양수;정종식;김수웅
    • Korean Journal of Veterinary Service
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    • v.22 no.1
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    • pp.79-84
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    • 1999
  • This study was carried out to investigate isolation of Pasteurella multocida from pneumonic lungs of slaughtered animals in Kyungsan abattoir to examine the antimicrobial susceptibility and biochemical properties. The results were summarized as follows ; P multocida was isolated from 50(29.2% ) of the 171 pneumonic lungs collected individually from cattle(3/40), goats(0131) and pigs(47/100), All of the isolated P multocida showed biocemical and cultural properties similiar to reference strains. All isolates were very susceptible to AK, AM, ENR, CF, GM,07, whereas resistant to SDM.

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Biochemical properties and antimicrobial drug susceptibility of Pasteurella haemolytica isolated from pneumonic calves and goats (호흡기 증상을 나타낸 송아지 및 산양에서 분리한 pasteurella haemolytica의 생화학적 특성 및 약제감수성)

  • Cho, Kwang-hyun;Kim, Bong Hwan
    • Korean Journal of Veterinary Research
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    • v.31 no.4
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    • pp.449-456
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    • 1991
  • The present study was conducted to investigate biochemical properties and antimicrobial drug susceptibilities of 57 strains of Pasteurella haemolytica (P haemolytica) isolated from pneumonic calves and goats in Youngnam province during the period from September 1989 to February 1991. P haemolytica was isolated from 95 of 142(31.7%) pneumonic calves of 1 to 6 months of age and from 9 of 24(37.5%) pneumonic goats of 2 to 10 months of age. Seasonal isolation frequency of P haemolytica in calves and goats varied from 16.6 to 35.7%, and it was higher in winter. The majority of biochemical and cultural properties of P haemolytica isolated from calves and goats were identical to those of the reference strains employed. All isolates were susceptible to ceftiofur, erythromycin, kanamycin, gentamicin, and chloramphenicol (MIC: <$25{\mu}g/ml$), some of them were resistant to amikacin, sulfadimethoxin, and streptomycin (MIC: >200IU or ${\mu}g/ml$).

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Biochemical properties and serotypes of pathogenic Escherichia coli isolated from poultry in Korea (가금 유래 병원성대장균의 생화학적 성상 및 혈청형)

  • Sung, Myung-Suk;Kim, Jin-Hyun;Ha, Jong-Su;Cho, Jae-Keun;Seol, Sung-Yong;Kim, Ki-Seuk
    • Korean Journal of Veterinary Research
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    • v.48 no.2
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    • pp.145-151
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    • 2008
  • This study was conducted to investigate biochemical properties and O group serotypes of pathogenic 203 Escherichia (E.) coli isolates from poultry with collibacillosis in Korea during the period from April 2003 to December 2005. Biochemical and fermentative properties of 203 isolates of E. coli tested were in accordance with Cowan and Steel's classification standard. One hundred and forty one isolates (69.5%) could be classified into a total of 20 O serotypes. Among them, the predominant O groups were O78 (32.5%), O88 (7.8%), O15 (6.8%), O141 (6.4%), and O158 (3.0%) in decreased order. Other infrequently encountered serogroups included : O8 (2%), O161 (2%), O20 (1.5%), O125 (1.5%), O2 (1%). And O6, O18, O24, O46, O76, O109, O119, O138, O139 and O148 had a frequency of 0.5%, respectively. Sixty two isolates (30.5%) were non-typeable with standard 173 O antisera used in this study.

Characterization of Biochemical Properties of Feline Foamy Virus Integrase

  • Lee, Dong-Hyun;Hyun, U-Sok;Kim, Ji-Ye;Shin, Cha-Gyun
    • Journal of Microbiology and Biotechnology
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    • v.20 no.6
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    • pp.968-973
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    • 2010
  • In order to study its biochemical properties, the integrase (IN) protein of feline foamy virus (FFV) was overexpressed in Escherichia coli, purified by two-step chromatography, (Talon column and heparin column), and characterized in biochemical aspects. For the three enzymatic reactions of the 3'-processing, strand transfer, and disintegration activities, the $Mn^{2+}$ ion was essentially required as a cofactor. Interestingly, $Co^{2+}$ and $Zn^{2+}$ ions were found to act as effective cofactors, whereas other transition elements such as $Ni^{2+}$, $Cu^{2+}$, $La^{3+}$, $Y^{3+}$, $Cd^{2+}$, $Li^{1+}$, $Ba^{2+}$, $Sr^{2+}$, and $V^{3+}$ were not. Regarding the substrate specificity, FFV IN has low substrate specificities as it cleaved in a significant level prototype foamy virus (PFV) U5 LTR substrate as well as FFV U5 LTR substrate, whereas PFV IN did not. Finally, the 3'-processing activity was observed in high concentrations of several solvents such as CHAPS, glycerol, Tween 20, and Triton X-100, which are generally used for dissolution of chemicals in inhibitor screening. Therefore, in this first report showing its biochemical properties, FFV IN is proposed to have low specificities on the use of cofactor and substrate for enzymatic reaction as compared with other retroviral INs.