• Title, Summary, Keyword: biopolymer production

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Development of continuous process for the production of Exo-biopolymer with Cordyceps militaris

  • Kim, Kyoung-Ju;Lee, Jung-Heon
    • 한국생물공학회:학술대회논문집
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    • pp.243-245
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    • 2003
  • The continuous fermentation process for exe-biopolymer production with Cordyceps militaris has been developed in this research. With the change of dilution rate, biopolymer production has been changed and the maximal biopolymer production has been achieved when the dilution rate was 0.0225/hr. The maximum mycelium concentration and biopolymer concentration were 8 g/L and 4.2 g/L, respectively.

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Effect of fermentor type on biopolymer production with Cordyceps militaris

  • Kim, Kyoung-Ju;Kim, Dong-Hyun;Park, Yang-Ho;Kwun, Kyu-Hyuk;Lee, Jung-Heon
    • 한국생물공학회:학술대회논문집
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    • pp.249-251
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    • 2003
  • The effects of fermentor types on biopolymer production with Cordyceps militaris has been studied in this articles. Since the shear stresses of stirred tank ferementor and bubble column fermentor were different, the mycelium growth and biopolymer production were different. The mycerial growth was high with the bubble column fermentor for the early stage but biopolymer production was lower than that with stirred tank fermentor. The production of biopolymer was closely related to shear stress to the cells and biopolymer production was increased with increased shear stress.

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Morphological Variation of Enterobacter sp. BL-2 in Acetate-mediated pH Environment for Excretive Production of Cationic Microbial Polyglucosamine Biopolymer

  • Son, Mi-Kyung;Hong, Soo-Jung;SaGong, Kuk-Hwa;Lee, Yong-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.18 no.1
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    • pp.104-106
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    • 2008
  • Enterobacter sp. BL-2 excretively produced a unique cationic polyglucosamine biopolymer PGB-1 comprised of more than 95% D-glucosamine in an acetate-mediated culture condition. The excretion of the biopolymer PGB-1 was closely associated with the cellular morphology of Enterobacter sp. BL-2, a feature highly dependable on the pH of the medium. The initially formed uneven and irregular surface cells were aggregated into the cell-biopolymer network structure connected by the adhesion modules of the cell-bound biopolymer. The excretive production of the biopolymer PGB-1 coincided with the disruption of the cell-biopolymer network, most actively at the medium pH of 8.0.

Production Conditions and Characterization of the Exo-biopolymer Produced by Submerged Cultivation of Ganoderma lucijum Mycelium (영지(Ganoderma lucidum) 균사체의 액체배양에 의한 세포외 생물고분자의 생산조건과 특성)

  • Lee, Shin-Young;Kang, Tae-Su
    • Microbiology and Biotechnology Letters
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    • v.24 no.1
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    • pp.111-118
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    • 1996
  • For the screening and the development of the new bio-material, cultural conditions for the exo-biopolymer (EBP) production throught the submerged cultivation of Ganoderma lucidum mycelium were investigated. Also, the fractionations and the purifications of the exo-biopolymer were carried out and the chemical compositions of the exo-biopolymer were examined. The optimal culture conditions for the exo-biopolymer production were pH 5.0, 30$^{\circ}C$ and 100 rpm of agitation speed in the medium containing of 5% (w/v) glucose, 0.5%(w/v) yeast extract, 0.1% (w/v) ($(NH_4)_2HPO_4$, and 0.05% (w/v) $KH_2PO_4$. In the flask cultivation for 7 days under these conditions, the concentration of the maximum exo-biopolymer and the cell mass were 15.4g/l and 18.8g/l, respectively. The specific growth rate was 0.039 $hr^{-1}$. In addition, the substrate consumption rate, and the exo-biopolymer production rate were 0.043$gg^{-1}$$hr^{-1}$ and 0.025$gg^{-1}$$hr^{-1}$, respectively. The exo-biopolymer was fractionated into BWS (water soluble exo-biopolymer) and BWI (water insoluble exo-biopolymer) by the water extraction, and the sugar contents of two fractions were higher than 97% (based on dry basis). The components sugar of BWS and BWI fractions were glucose, galactose, mannose, xylose, and fucose. Their molar ratios were 3.6:1.5:2.1:0.5: trace and 2.9:3.1:2.0:1.6:0.3, respectively.

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Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps millitaris

  • Park, Jong-Pil;Sinha, Jayanta;Song, Chi-Hyeon;Yun, Jong-Won
    • 한국생물공학회:학술대회논문집
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    • pp.317-320
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    • 2000
  • The optimal temperature and pH for both mycelial growth and exe-biopolymer production by Cordyceps millitaris in shake flask culture were found to be $20^{\circ}C$ and 6.0, respectively. Sucrose (4%) and corn steep powder (1%) were the most suitable carbon and nitrogen source for mycelial growth and exo-biopolymer production. The maximum specific growth rate $(0.142h^{-1})$ was achieved when sucrose was used as the sole carbon source. Exo-biopolymer production was increased with the increase in C/N molar ratio concentration, probably due to the facilitated carbon uptake. Under the optimal culture conditions, the maximum mycelial growth exe-biopolymer concentration were reached to around 13.3 g dry cell weigh/l and 3.33 g/l, respectively.

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Optimization of Submerged Culture Conditions for Exo-biopolymer Production by Paecilomyces japonica

  • Bae, Jun-Tae;Sinha, Jayanta;Yun, Jong-Won
    • 한국생물공학회:학술대회논문집
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    • pp.199-202
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    • 2000
  • Optimization of submerged culture conditions for the production of exo-biopolymer from Paecilomyces japonica was studied. Maltose, yeast extract and potassium phosphate were the most suitable sources of carbon, nitrogen, and inorganic salt, respectively, for both production of the exo-biopolymer and mycelial growth. The optimal culture conditions in flask culture were pH 5.0, $25^{\circ}C$ and 150 rpm in a meidum containing of 30 g maltose, 6 g yeast extract, 2 g polypeptone, 0.5 g $K_2HPO_4$, 0.2 g $KH_2PO_4$, 0.2 g $MnSo_4\;{\cdot}\;5H_2O$, 0.2 g $MgSO_4\;{\cdot}\;7H_2O$ in 1-L distilled water. Exo-biopolymer production and mycelial growth in the suggested medium were significantly increased in a 2.5-L jar fermentor, where the maximum biopolymer concentration was 8 g/1.

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Production Condition and Utilities of Extracellular Biopolymer from Bacillus licheniformis (Bacillus licheniformis가 생산하는 Extracellular Biopolymer의 생산조건 및 이용특성)

  • 진효상;이완옥
    • Korean Journal of Environmental Biology
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    • v.18 no.1
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    • pp.199-203
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    • 2000
  • A bacterium that produce biopolymer was isolated from Gochujang, one of Korean traditional fermented foods, and identified as Bacillus licheniformis. The production of biopolymer was highest and 34mg/250ml, when the baterium was cultivated in condition of sucrose 6.0%, Yeast extract 0.1%, peptone 0.l%, NaCl 3.0%, and pH 6.0. The 1% solution of this biopolymer was able to form a translucent and glossy film. And the solution of biopolymer was found to precipitate Kaolin solution and also stabilize the suspension of lactic acid bacteria within the test range of 0.25-1.00%. [Bacillus licheniformis, Biopolymer].

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Optimazation of Submerged Culture Conditions for Exo-Biopolymer Production by Paecilomyces japonica

  • Bae, Jun-Tae;Sinha, Jayanta;Park, Jong-Pil;Song, Chi-Hyun;Yun, Jong-Won
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.482-487
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    • 2000
  • Optimization of submerged culture conditions for the production of exo-biopolymer from Paecilomyces japonica ws studied. Maltose, yeast extract, and potassium phosphate were the most suitable sources of carbon, nitrogen, and inorganic salt, respectively, for both production of the exo-biopolymer and mycelial growth. The optimal culture conditions in a flask culture were pH 5.0, $25^{\circ}C$, and 150 rpm in a medium containing (as in g/l) 30 maltose, 6 yeast extruct, 2 polypeptone, $0.5{\;}K_3HPO_4,{\;}0.2{\;}KH_2PO_4,{\;}0.2{\;}MnSO_4{\cdot}5H_2O,{\;}0.2{\;}MgSO_4{\cdot}7H_2O$. Exo-biopolymer production and mycelial growth in the above suggested medium were significantly increased in a 2.5-1 jar fermentor, where the maximum biopolymer concentration was 8 g/l. The morphological changes of the mycelium in the submerged culture were observed within pH ranges from 4.0 to 9.0; i.e., growth of the filamentous form was optimal at culture pHs of 5.0 and 6.0, whereas pellet was formed at other pHs.

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Optimization of Biopolymer Production from Alkali-Tolerant Bacillus sp. (알칼리 내성 Bacillus sp.의 생물고분자 생산조건의 최적화)

  • Lee, Shin-Young;Lee, Beom-Su;Lee, Keun-Eok
    • Korean Journal of Food Science and Technology
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    • v.23 no.2
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    • pp.167-174
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    • 1991
  • Cultural conditions for the biopolymer production by an alkali tolerant Bacillus sp. isolated from soil were investigated and determination of optimal conditions was carried out by response surface method. The maximal production of biopolymer was obtained after cultivation at $30^{\circ}C$ for 36hrs in the mixture of 8% soluble starch, 0.75% yeast extract, 0.1% $NaNO_3$, 0.05% $MgSO_4\;7H_2O$ and 1% $Na_2CO_3$ adjusted to pH 10. Under these conditions, about 44 g/l of biopolymer were produced. From the results of response surface analysis, optimal condition for the production of biopolymer were obtained at stationary point with 15.16 of C/N ratio, $34.62^{\circ}C$ of temperature and 9.50 of pH. On the basis of these conditions, it was estimated that 66.84 g/l of the biopolymer could be produced.

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Changes in Rheological Properties of Culture Broth During the Biopolymer Production by Bacillus sp (Bacillus sp.에 의한 생물 고분자의 발효 중 배양액의 유변학적 특성 변화)

  • 이신영;이주하
    • KSBB Journal
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    • v.11 no.3
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    • pp.340-346
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    • 1996
  • Variations of rheological properties of culture broth during the production of biopolymer by an alkali tolerant Bacillus sp. were investigated. Correlations among the rheological characteristics of culture broth, cell growth and biopolymer production were examined. Rheology of the culture broth changed in the course of fermentation. The culture broth showed a non-Newtonian flow behavior, as the viscosity and pseudoplasticsity increased during the cultivation. The rheological parameters such as flow index, consistency index, yield stress and apparent viscosity during the cultivation were not influenced by the cell growth, but significantly related to the biopolymer synthesis. Changes in the rheological parameters of the broth were affected not only by the biopolymer concentration, but also by the progress of fermentation. Some rheological parameters showed maximum values just before the completion of biopolymer production and substrate consumption. Hence, it was shown that the rheological characteristics of the culture broth could be used as a good indicator for the detection of the progress or completion of fermentation.

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