• Title, Summary, Keyword: bioreactor system

Search Result 277, Processing Time 0.044 seconds

Effect of Agitation and Aeration Rate on Nicotiana tabacum Suspension Cell Culture in Bioreactors (Bioreactor를 이용한 담배세포 현탁배양에서 교반형태와 통기량이 미치는 영향)

  • Lee, Sang-Yun;Kim, Dong-Il
    • KSBB Journal
    • /
    • v.14 no.5
    • /
    • pp.534-538
    • /
    • 1999
  • For the optimization of operating conditions for plant cell suspension culture in bioreactors, effects of bioreactor types, various kinds of impellers, and aeration rates were examined using Nicotiana tabacum cells as a model system. Stirred tank bioreactor and airlift bioreactor were used for the comparison of bioreactor type. Growth rates in both bioreactors were lower than in shake flasks. In terms of final cell concentration, stirred tank bioreactor supported a little bit better growth compared to airlift bioreactor. Impeller type did not affect cell growth significantly, but it was apparent that cell size index decreased in the case of using hollowed paddle impeller. When the aeration rate was maintained at 0.3 vvm, cell growth was the best. At above 1.0 vvm, growth inhibition as well a browning was noticed. In addition, it was found that cell size index reduced proportionally to the increased of aeration rate.

  • PDF

Development of a Novel Bioreactor System for the Treatment of Gaseous Benzene

  • Yeom, Sung-Ho;Daugulis, Andrew J.;Yoo, Young-Je
    • 한국생물공학회:학술대회논문집
    • /
    • /
    • pp.73-76
    • /
    • 2000
  • A novel, continuous bioreactor system combining a bubble column (absorption section) and a two-phase bioreactor (degradation section) has been designed to treat a gas stream containing benzene. The bubble column contained hexadecane as an absorbent for benzene, and was systemically chosen considering physical, biological, environmental, operational and economic factors. This solvent has infinite solubility for benzene and very low volatility. After absorbing benzene in the bubble column, the hexadecane served as the organic phase of the two-phase partitioning bioreactor, transferring benzene into the aqueous phase where it was degraded by Alcaligenes xylosoxidans Y234. The hexadecane was then continuously recirculated back to the absorber section for the removal of additional benzene. All mass transfer and biodegradation characteristics in this system were investigated prior to operation of the integrated unit, and these included: the mass transfer rate of benzene in the absorption column, the mass transfer rate of benzene from the organic phase into the aqueous phase in the two-phase bioreactor, the stripping rate of benzene out of the two-phase bioreactor, etc. All of these parameters were incorporated into model equations, which were used to investigate the effects of operating conditions on the performance of the system. Several experiments were conducted to show the feasibility of this system. This process is believed to be very practical for the treatment of high concentrations of gaseous pollutants.

  • PDF

Efficient Propagation by Bioreactor System of Korean Native Seosanjong in Ginger

  • Jo, Man-Hyun;Ham, In-Ki;Lee, Mi-Ae;Lee, Eun-Mo;Song, Nam-Hyun;Woo, In-Shik
    • Plant Resources
    • /
    • v.3 no.3
    • /
    • pp.219-222
    • /
    • 2000
  • For the purpose of establishing an efficient propagation through airlift bioreactor system of Zingiber of officinale Rosc. Korean native Seosanjong, the effect of different factors and bioreactor on cultured plantlets were investigated. The highest number of plantlets, fresh weight per plant was obtained from explants when cultured in MS liquid medium including 0.3 mg/L NAA and 2.0 mg/L kinetin for 40 days. A 10 L bottle type bubble bioreactor, compared with 250 mL Erlenmeyer flask, was more efficient, producing 4.7 plantlets or from 1.5 to 1.6 times more than did the Erlenmeyer flask. The results demonstrate the rapid mass propagation of airlift bioreactor to produce normal ginger.

  • PDF

Development of Bioreactors for Enrichment of Chemolithotrophic Methanogen and Methane Production (독립영양형 메탄생산세균의 농화 및 메탄생산 반응기의 개발)

  • Na, Byung-Kwan;Hwang, Tae-Sik;Lee, Sung-Hun;Ju, Dong-Hun;Sang, Byung-In;Park, Doo-Hyun
    • Microbiology and Biotechnology Letters
    • /
    • v.35 no.1
    • /
    • pp.52-57
    • /
    • 2007
  • A gas-circulating bioreactor was used for enrichment of autotrophic methanogens. Mixture of hydrogen and carbon dioxide (5:1) was used as a sole energy and carbon source. Anaerobic digestive sludge isolated from wastewater treatment system was inoculated into the gas-circulating bioreactor. The enrichment of two chemolithotrophic methanogens, Methanobacterium curvum and Methanobacterium oryzae was accomplished in the gas-circulating bioreactor. The enriched bacteria were cultivated in a bioreactor equipped with hollow-fiber hydrogen-supplying system (hollow-fiber bioreactor), and a hybrid-type bioreactor equipped with hollow-fiber hydrogen-supplying system and electrochemical redox control system. The methane productivity was maximally 30% (V/V) in the hollow-fiber bioreactors and 50% (V/V) in the hybrid-type bioreactor.

골조직 재생 촉진을 위한 Novel Perfusion Bioreactor

  • Lee, Si-U;Heo, Su-Jin;Jang, Ji-Yeon;Jeong, Jae-Yeong;Kim, Seung-Eon;Sin, Jeong-Uk
    • Proceedings of the Materials Research Society of Korea Conference
    • /
    • /
    • pp.46.2-46.2
    • /
    • 2009
  • In vitro 상에서 골조직을 원활하게 재생하기 위해서는 3차원 지지체를 이용한 세포 배양과 세포 배양 시 세포의 형태와 기능을 유지/향상시키기 위한 인체 내 미세 환경 재현은 필수적이다. 따라서 본 연구에서는 뼈 성분과 유사한 생체 활성 물질인 hydroxyapatite (HA)와 생분해성 고분자인 poly $\varepsilon$-caprolactone (PCL)를 복합재료로 이용하여 내부 연결성이 우수한 골조직 재생용 3차원 지지체를 제작하였으며, 골 재생 능력 향상을 위하여 인체내 골조직의 기계적 미세 환경을 체외에서 구현한 새로운 형태의 perfusion bioreactor system을 개발/적용하였다. 또한 본 연구에서 개발된 perfusion bioreactor system의 생물학적 평가를 위해 MG63 (osteoblast like cell, 한국 세포주 은행)과 New Zealand White Rabbit에서 분리한 중간엽 줄기세포를 골조직 재생용 3차원 지지체에 파종하였다. 48시간 동안 안정화 후 perfusion bioreactor system을 이용하여 기계적 자극을 파종된 세포에 인가하였으며, 배양 기간 동안 세포의 증식 확인 및 형태학적 관찰을 실시하였다. 본 연구 결과, perfusion bioreactor system을 이용하여 기계적 자극을 인가한 실험군에서 세포의 증식 및 활성도가 대조군에 비해 우수함을 확인 할 수 있었다. 따라서, perfusion bioreactor를 이용한 세포 배양은 세포의 활성 향상 및 골조직 재생에 도움이 될 것으로 사료된다. 차후 perfusion bioreactor를 이용한 다양한 패턴의 자극이 골재생 능력 및 중간엽 줄기세포의 골 분화능에 미치는 영향에 대한 연구가 필요할 것으로 사료된다.

  • PDF

Effects of Mechanical Stimulation for MC3T3-E1 Cells using Bioreactor (바이오리액터를 이용한 MC3T3-E1 세포의 기계적 자극에 대한 영향)

  • Lee, In-Hwan;Park, Jeong-Hun;Lee, Seung-Jae;Cho, Dong-Woo;Kang, Sang-Sun
    • Proceedings of the KSME Conference
    • /
    • /
    • pp.1411-1414
    • /
    • 2008
  • It is reported that mechanical stimulation takes a role in improving cell growth in skeletal system. And various research groups have showed that developed bioreactor to stimulate cell-seeded and threedimensional scaffold. In this study, we designed a custom-made bioreactor capable of applying controlled compression to cell-seeded agarose gel. This device consisted of a circulation system and compression system. In circular system, culture chamber was sealed for prohibiting contamination and media solution was circulated by pump. In compression system, mechanical stimuli were controlled by LabVIEW software and mechanical transfer system. Cell-encapsulated agarose gels were cultured for up to 7 days. There were significant differences between the number of cells grown in dynamic cell culture and in static cell culture from 3 days to 7 days.

  • PDF

Development of Bioreactor by Rapid Prototyping Technology (쾌속 조형 기술을 이용한 바이오리액티의 개발)

  • Park, Jeong-Hun;Lee, Seung-Jae;Lee, In-Hwan;Cho, Dong-Woo;Rhie, Jong-Won
    • Journal of the Korean Society for Precision Engineering
    • /
    • v.26 no.3
    • /
    • pp.137-143
    • /
    • 2009
  • It has been reported that mechanical stimulation takes a role in improving eel/ growth in skeletal system. Various research groups have been showed their own bioreactors which stimulate cell-seed three-dimensional scaffold. In this study, we hypothesized that the various conditions of mechanical stimulation would affect cell growth and proliferation. To prove our hypothesis, we designed a custom-made bioreactor capable of applying controlled compression to cell-encapsulated scaffolds. This device consisted of a circulation system and a compression system. Each parts of the bioreactor was fabricated using the rapid prototyping technology By using the rapid prototyping technology, we can modify and improve the bioreactor very rapidly For dynamic cell-culture, cell-encapsulated agarose gel was fabricated in 2% concentration. We performed dynamic cell-culture using this agarose gel and developed bioreactor in 3 days.

Application of a Membrane Bioreactor in Denitrification of Explosives Hydrolysates (Membrane Bioreactor를 이용한 폭발성 물질의 가수분해 부산물의 탈질과정에의 적용)

  • Zoh, Kyung-Duk
    • Journal of Korean Society on Water Environment
    • /
    • v.18 no.2
    • /
    • pp.113-122
    • /
    • 2002
  • A bench-scale anoxic membrane bioreactor (MBR) system, consisting of a bioreactor coupled to a ceramic crossflow ultrafiltration module, was evaluated to treat a synthetic wastewater containing alkaline hydrolysis byproducts (hydrolysates) of RDX, The wastewater was formulated the same as RDX hydrolysates, and consisted of acetate, formate, formaldehyde as carbon sources and nitrite, nitrate as electron accepters. The MBR system removed 80 to 90% of these carbon sources, and approximately 90% of the stoichiometric amount of nitrate, 60% of nitrite. The reactor was also operated over a range of transmembrane pressures, temperatures, suspended solids concentration, and organic loading rate in order to maximize treatment efficiency and permeate flux. Increasing transmembrane pressure and temperature did not improve membrane flux significantly. Increasing biomass concentration in the bioreactor decreased the permeate flux significantly. The maximum volumetric organic loading rate was $0.72kg\;COD/m^3/day$, and the maximum F/M ratio was 0.50 kg N/kg MLSS/day and 1.82 kg COD/kg MLSS/day. Membrane permeate was clear and essentially free of bacteria, as indicated by heterotrophic plate count. Permeate flux ranged between 0.15 and $2.0m^3/m^2/day$ and was maintained by routine backwashing every 3 to 4 day. Backwashing with 2% NaOCl solution every fourth or fifth backwashing cycle was able to restore membrane flux to its original value.

Nonlinear Adaptive Control of Fermentation Process in Stirred Tank Bioreactor

  • Kim, Hak-Kyeong;Nguyen, Tan-Tien;Nam soo Jeong;Kim, Sang-Bong
    • Transactions on Control, Automation and Systems Engineering
    • /
    • v.4 no.4
    • /
    • pp.277-282
    • /
    • 2002
  • This paper proposes a nonlinear adaptive controller based on back-stepping method for tracking reference substrate concentration by manipulating dilution rate in a continuous baker's yeast cultivating process in stirred tank bioreactor. Control law is obtained from Lyapunov control function to ensure asymptotical stability of the system. The Haldane model for the specific growth rate depending on only substrate concentration is used in this paper. Due to the uncertainty of specific growth rate, it has been modified as a function including the unknown parameter with known bounded values. The substrate concentration in the bioreactor and feed line are measured. The deviation from the reference is observed when the external disturbance such as the change of the feed is introduced to the system. The effectiveness of the proposed controller is shown through simulation results in continuous system.

Automation of Solid-state Bioreactor for Oyster Mushroom Composting

  • Lee, Ho-Yong;Kim, Won-Rok;Min, Bong-Hee
    • Mycobiology
    • /
    • v.30 no.4
    • /
    • pp.228-232
    • /
    • 2002
  • This study focused on the production of high quality compost for the growth of aero-thermophilic fungi, which has a promoting effect on the growth rate and production of oyster mushrooms. The automated solid-state bioreactor system was designed on the basis of a Three-Phase-One system, which controls the serial steps of prewetting, pasteurization and fermentation processes. High numbers of thermophilic fungi and bacteria were recovered from the mushroom composts prepared by this solid-state bioreactor. The rates of composting process were depended on physical as well as chemical factors. Among these factors, the parameters of moisture content and temperature were found to be particularly important. In our automated system, constant levels of moisture content, temperature and ventilation via mixing were provided by a centralized control apparatus including PLC, water tank and water jacket systems. These features induced higher microbiological activity of aero-thermophiles.