• Title, Summary, Keyword: biosensor

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Development of PZT Piezoelectric Biosensor for the Detection of Formaldehyde (Formaldehyde 측정을 위한 PZT 압전 바이오센서 개발)

  • 김병옥;곽성곤;임동준
    • KSBB Journal
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    • v.13 no.5
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    • pp.477-482
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    • 1998
  • A biosensor with PZT piezoelectric ceramic crystal was developed for the detection of formaldehyde gas. Poled PZT piezoelectric ceramic disk was made from ZrO2, TiO2 and Nb2O5, together with the addition of PbO and polyvinyl alcohol, through various processes of mixing, calcination drying, crushing, forming, sintering, polishing, ion coating and poling. Oscillator circuit of sensor was made of operational amplifier(AD811AN). Formaldehyde dehydrogenase was immobilized onto a piezoelectic ceramic crystal, together with the cofactors, reduced glutathione and nicotinamide adenine dinucleotide. The effect of flow rate on the sensitivity was determined by varing the flow rate of carrier gas from 24.7mL/min to 111.7mL/min through detector cell. The results indicated that as the flow rate was increased, the recovery rate was increased. And a significant increase in the sensitivity was observed in enhanced flow rate of carrier gas. Frequency difference(ΔF) of immobilized PZT piezoelectic disk increased proportionally to the concentration gas and reproduced to repeated exposures of formaldehyde gas(28ppm, Δ68Hz).

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Development of Measuring instrument module for Biosensor Activity using CMOS Image sensor and sensor network (센서 네트워크와 CMOS 이미지 센서를 이용한 바이오센서 활동량 측정 모듈 개발)

  • Park, Se-Hyun;Kak, Ho-Hjub;Kim, Eung-Soo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • pp.898-901
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    • 2008
  • Measuring instrument module for biosensor activity is developed using CMOS image sensor and sensor network. Most of living organism in water as water flea, fish, etc are frequently used as biological sensor for monitoring the water qualify. The activity of biosensor is changed by the quality of water. The developed measuring instrument module can easily interface to the existing instrument.

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Measurement of Sucrose Concentration Using a μFIA Biosensor (μFIA 바이오센서를 이용한 자당 농도 측정)

  • Song D. B.
    • Journal of Biosystems Engineering
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    • v.29 no.6
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    • pp.553-557
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    • 2004
  • A microdialysis coupled flow injection amperometric $({\mu}FIA)$ biosensor was calibrated to measure the concentration of sucrose using 11 standard samples from 2 ml to 70 ml of sucrose solution. The output of the sensor increased linearly with an increase in the sucrose concentration with an $r^2$ correlation of 0.99. The amperometric biosensor was then applied to measure the sucrose concentration of 4 commercial samples (Orange and Pineapple juices, Pepsi, Sprite) and the results compared with those by HPLC. Around $20\~30\%$ error was observed in sucrose concentration measurements of the samples analyzed. The sensor has potential in rapid measurement once the calibration is done. Potential for on-line sensing is also discussed.

Performance Evaluation of Flow Injection Type Biosensor According to Operating Variables of Pump (펌프의 작동요인에 따른 흐름주입식 바이오센서의 성능 검증)

  • Song D. B.;Jung H. S.;Lee S. K.
    • Journal of Biosystems Engineering
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    • v.30 no.5
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    • pp.312-317
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    • 2005
  • A flow injection type biosensor was tested to confirm the performance of a batch and a continuous type flow injection unit. Reproducibility and consistence of the biosensor were investigated to determine the effect of pulsations and air bubbles, and the applicability of on-line monitoring. The air bubbles affected the performance of the sensor irrespective of the location, and also the pulsations of the pump influenced the performance of the sensor. The applicability of on-line motoring was accepted as the result of the repeated and long-term measurements.

Development of an Enzyme Electrode Biosensor for Lactic Acid Bacteria (효소 전극을 이용한 유산균 측정 바이오센서 개발)

  • Park T. S.;Cho S. I.
    • Journal of Biosystems Engineering
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    • v.30 no.4
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    • pp.249-253
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    • 2005
  • This study was carried out to develop enzyme biosensor for lactic acid bacteria. Lactic acids produced by lactic acid bacteria (LAB) was measured and good correlation $R^2=0.98$ between LAB count and lactic acids concentration was found. Hydrogen ion produced by L-lactate dehydrogenase (L-LDH) was measured by a potentiometer. Glutamic-pyruvic transminase (GPT) was used for eliminating inhibitor in the reaction. Polyacrylamide gel was used for immobilizing matrix of the sensor. The biosensor was tested and showed good feasibility with $R^2=0.99$ on validation.

Fabrication of Polyimide Film Electrode by Laser Ablation and Application for Electrochemical Glucose Biosensor (Laser ablation을 이용한 폴리이미드 필름 전극제조 및 전기화학적 글루코오즈 바이오센서 응용)

  • Park, Deog-Su
    • Journal of Sensor Science and Technology
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    • v.22 no.5
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    • pp.357-363
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    • 2013
  • An ultraviolet pulsed laser ablation of polyimide film coated with platinum has been used to enhance the sensitivity for the application as an electrochemical biosensor. Densely packed cones are formed on polyimide surface after UV irradiation which results in increase of surface area. In order to apply the sensitivity improvement of laser ablated polyimide film electrodes, the glucose oxidase modified biosensor was fabricated by using an encapsulation in the gel matrix through sol-gel transition of tetraethoxysliane on the surface of laser ablated polyimide film. The optimum conditions for glucose determination have been characterized with respect to the applied potential and pH. The linear range and detection limit of glucose detection were from 2.0 mM to 18.0 mM and 0.18 mM, respectively. The sensitivity of glucose biosensors fabricated with laser ablated polyimide film is about three times higher than that of plain polyimide film due to increase in surface area by laser ablation.

Development of Glucose Biosensor Using Sol-Gel Reaction of Tetraethoxysilane (Tetraethoxysilane의 졸-겔 반응을 이용한 전기화학적 glucose biosenor 개발)

  • Chang, Seong-Cheol;Park, Deog-Su
    • Journal of Sensor Science and Technology
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    • v.21 no.4
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    • pp.311-317
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    • 2012
  • Disposable amperometric screen-printed biosensor strips have been fabricated by a sol-gel encapsulation for the analysis of glucose. The glucose oxidase(GOx) is entrapped in the gel matrix through sol-gel transition of tetraethoxysliane(TEOS). The biosensor is fabricated by GOx containing thin film of TEOS gel on the surface of screen-printed carbon electrode(SPCE). The GOx-containing thin film of TEOS gel offers a one-step modification process on the surface of SPCE. The optimum conditions for glucose determination have been characterized with respect to the applied potential, enzyme loading ratio, and pH. The linear range and detection limit of glucose detection were from 2.0 mM to 16.0 mM and 0.25 mM, respectively.