• Title, Summary, Keyword: biotype

Search Result 141, Processing Time 0.167 seconds

Isolation and identification of Agrobacterium spp. using carrot disc and transformation of Nicotiana gluca by selected strains (당근 절편을 이용한 Agrobacterium spp. 분리, 동정과 선발균주에 의한 연초의 형질전환)

  • Yang, Deok-Chun;Yang, D.C.;Choi, K.T.
    • Journal of the Korean Society of Tobacco Science
    • /
    • v.13 no.1
    • /
    • pp.43-52
    • /
    • 1991
  • These studies were carred out to obtain the transformant from tobacco cells by Agrobacterium spp. from crown gall and soil at the natural field in Korea, and identified their virulence. Kodo's and Clark's selective media were used for isolation of Agrobacterium spp. In these media, total of 99 strains were characterized based on the morphological characteristics of colonies. Among them 34 strains were able to induce on carrot discs. And hypervirulent strains C23-1 and K29-1 were identified as Agrobacterium tumefaciens biotype 1 and biotype 2, respectively. These strains formed fast growing, larger gall as compared to those induced by other strains on the carrot discs. Transformed tobacco callus was initiated on the phytohormone free MS medium with 250$\mu\textrm{g}$/ml carbenicillin after co-cultivation of tobacco stem explants and Agrobacteria. On the phytohormone free media, shoot was rarely formed from transformed callus. However, these shoot were teratoma shoots which were not grown as normal shoot, and teratoma shoot from transformant by C23-1 was smaller than that of K29-1.

  • PDF

Expressed Sequence Tags of the Wheat-rye Translocation Line Possessing 2BS/2RL

  • Jang, Cheol-Seong;Hong, Byung-Hee;Seo, Yong-Weon
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.44 no.3
    • /
    • pp.302-307
    • /
    • 1999
  • Hamlet (PI549276) possessing 2RL was obtained by cross between a wheat cultivar ND7532 (Froid/Centurk) and a rye cultivar Chaupon. Chaupon was known to have resistant gene to biotype L of Hessian fly [Mayetiola destructor (Say)] larvae. The wheat-rye translocation line (Coker797*4/Hamlet) was also known to be resistant to biotype L of Hessian fly larvae. We analysed a set of 96 ESTs from the wheat-rye translocation line (2BS/2RL). ESTs were classified by various physiological processings, such as primary metabolism, secondary metabolism, transcription, translation, transport, signal transduction, defense, transposable element, and others. Three sequences encoding thioredoxin peroxidase, 26S rRNA, and rubisco small subunits were homologous to registered genes in rye. Although limited number of clones were used to develop ESTs, these clones and their sequence information may be useful for researchers studying general physiology and molecular biology on the translocation line.

  • PDF

The significance of soft tissue for maintenance of prosthesis (보철 건강유지를 위한 연조직의 중요성)

  • Kim, Ok-Su
    • The journal of the Korean dental association
    • /
    • v.48 no.9
    • /
    • pp.664-669
    • /
    • 2010
  • All prosthetic and restorative therapies require a healthy periodontium as a prerequisite for success. Understanding of the concepts of periodontal-restrorative interaction, especially with regard to interactions at the gingival margin is important. The aim of this article gives the information about the essential considering factor for successful prosthesis; biologic width, periodontal biotype, width of attached gingiva, margin of restoration. If a restorative margin must be extended below the gingival margin, it is critical that adequate band of attached gingiva is present, the margin does not violate the biologic width, the margin is closed and properly finished.

Studies on the Pathogenic Test of Yersinia enterocolitica (Yersinia enterocolitica 의 병원성 검정에 관한 연구)

  • Lim, Soon-Young;Yoon, Suk-Kwon
    • Korean Journal of Food Science and Technology
    • /
    • v.33 no.4
    • /
    • pp.486-491
    • /
    • 2001
  • The pathogenicity for one hundred strains of domestic and foreign Y. enterocolitica was tested with HEp-2 cell invasion method as a reference. The serotyping, biotyping, PCR and esculin hydrolyis, salicin fermentation, pyrazinamidase activity, indole production, xylose fermentation, CRMOX and autoagglutination were compared to determine the possibility of pathogenic detection method. According to the test results, serotyping was limited to verify pathogenicity, however, biotyping was quite related to pathogenicity up to 99%. The biotype 1A strains were non-pathogenic, while all strains of biotype $1B{\sim}4$ showed pathogenicity with the exception of one strain belonged to type 1B. The esculin and salicin test results were completely close and correlated to pathogenicity up to 99%. The HEp-2 cell invasion and pyrazinamidase test were related to pathogenicity by 95%. Biochemical tests such as D-xylose fermentation, CRMOX agar test and autoagglutination in broth were effective as a support test. It is strongly recommended that sequencial esculin test and PCR test could be done to verify pathogenicity of Y. enterocolitica as the easiest and accurate procedure.

  • PDF

Relationship between the Caries Experience of Korean School Children and the Distribution of Streptococcus mutans in Dental Plaque (한국아동(韓國兒童)의 치아우식경험과 치면상(齒面上) Streptococcus mutans 분포(分布)에 대(對)한 연구(硏究))

  • Kim, Kack-Kyun;Choe, Son-Jin;Lim, Chang-Yoon;Chang, Woo-Hyun
    • The Journal of the Korean Society for Microbiology
    • /
    • v.18 no.1
    • /
    • pp.11-21
    • /
    • 1983
  • Various investigations have been carried out to elucidate the causative relationship between specific oral bacterial species and dental caries since it was first demonstrated that selected streptococoal species produced dental caries in germfree rats when fed a high-sucrose diet. Now, S. mutans is considered to play an important role in the development of dental caries in animals and humans, and only a limited number of species of bacteria other than S. mutans are occasionally found to be cariogenic in experimental animals. In this regard, association of the number of S. mutans in approximal plaque with caries experience (DMFT) was studied from 137 Korean school children($10{\sim}11$ year old). Biotypes of the collected strains of S. mutans were determined, and their relationship with caries status was also examined. The following results were emerged from the study. 1. S. mutans was detected in the plaques of all children. 2. Statistically significant positive correlation(r=0.445, p<0.001) was found between the caries experience(DMFT) and the number of S. mutans in approximal plaques. 3. The number of S. mutans were significantly higher(p<0.001) in plaques removed from carious surface than from sound surface. 4. The most frequent biotype was biotype I(78.8%), followed by IV(33.1%) and V(09.5%). Biotype II was isolated in the plaque of two children(1.7%) only. 5. There was no apparent relation of specific biotypes to carious status.

  • PDF

Periodontal biotype modification using a volume-stable collagen matrix and autogenous subepithelial connective tissue graft for the treatment of gingival recession: a case series

  • Kim, Hyun Ju;Chang, Hyeyoon;Kim, Sungtae;Seol, Yang-Jo;Kim, Hyeong-Il
    • Journal of Periodontal and Implant Science
    • /
    • v.48 no.6
    • /
    • pp.395-404
    • /
    • 2018
  • Purpose: The purpose of this study was to propose a technique for periodontal biotype modification through thickening of the entire facial aspect using a volume-stable collagen matrix and autogenous subepithelial connective tissue graft (CTG) for the treatment of gingival recession. Methods: Four systemically healthy patients showing Miller class I or class II gingival recession in the mandibular incisor area were included in this study. Full-mouth scaling and root planing procedures were performed at least 4 weeks prior to periodontal plastic surgery. A split-thickness flap with a horizontal intrasulcular incision and 2 vertical incisions was used in cases 1-3, and the modified tunnel technique was used in case 4 for coronal advancement of the mucogingival complex. After the exposed root surfaces were debrided thoroughly, double-layered volume-stable collagen matrix was placed on the apical part of the recession and a subepithelial CTG harvested from the palatal area was placed on the coronal part. The amount of root coverage at 3 months postoperatively was evaluated in cases 1-3, and facio-lingual volumetric changes were analyzed in cases 1 and 2. Results: Healing was uneventful in all 4 cases and complete root coverage was shown in cases 1-3. In case 4, reduction of gingival recession was observed at 3 months after surgery. In cases 1 and 2, a comparison of stereolithographic files from the preoperative and postoperative time points demonstrated that the entire facio-lingual volume had increased. Conclusions: The surgical technique suggested herein, using a volume-stable collagen matrix and autogenous subepithelial CTG, may be an effective method for periodontal biotype modification through thickening of the entire facial aspect for the treatment of gingival recession.

Studies on the brucellosis in Kyongbuk area (경북지역의 부루셀라병에 관한 연구)

  • 박노찬;김상윤;조광현;도재철;김영환;신상희;조민희;오강희;김우현
    • Korean Journal of Veterinary Service
    • /
    • v.21 no.4
    • /
    • pp.451-465
    • /
    • 1998
  • The present study was carried out to investigate the prevalence of brucellosis in Kyungbuk area for the 3 years from 1966 to 1998. Collective milk samples were routinely screened to detect positive farms by using the milk ring test(MRT), and serum agglutination test was performed to detect sero-positive individuals in the MRT positive farms. Attempt were made to isolate the causative organismas from slaughtered sero-positive reactors and some biochemical and polymerase chain reation characters of the isolates were also made to identify the organisms. Seroprevalence to brucellosis in peoples who are close contact with infected dairy herds was also investigated. Brucellosis of dairy cattle was rare before 1997, but has been broken more frequently since early 1998. By the MRT for dairy herds, positive rate was gradually increased every year : 0.6% in 1996, 1.5% in 1997, 3.9% in 1998. Among 262 MRT-positive herds, only 21 herds(8.0%) showed positive brucellosis in serological test. The isolation rates of Brucella sp from tested materials were 51.2% in supramammary glands, 39.5% in milks, and 50.0% in pulmonary Iymphnode, respectively. Isolated strain and biotype were Brucella(B) arbortus biotype 1 in 26 heads, and were B suis biotype 1 in 2 heads. Isolated strain and vaccine strain were very similar in their colony morphology and staining. In drug susceptibility, isolated stains(B abortus) and vaccine strain(B abortus RB-51) were sensitive to ampicillin, gentamycin, kanamycin, neomycin, penicillin, streptomycin, and to tetracycline, but resistant to erythromycin. In the PCR, field strains reacted to BA and IS711 primers, and vaccine strain reacted to BA, IS711, and RB5l primers. In the plate agglutination test of 96 sera of human contacted with animals, serum antibody titer detected 1 : 100 in one person, 1 : 200 in one, and below 1 : 25 in the others.

  • PDF

Effectiveness of Rhizobacteria Containing ACC Deaminase for Growth Promotion of Peas (Pisum sativum) Under Drought Conditions

  • Zahir, Z.A.;Munir, A.;Asghar, H.N.;Shaharoona, B.;Arshad, M.
    • Journal of Microbiology and Biotechnology
    • /
    • v.18 no.5
    • /
    • pp.958-963
    • /
    • 2008
  • A series of experiments were conducted to assess the effectiveness of rhizobacteria containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase for growth promotion of peas under drought conditions. Ten rhizobacteria isolated from the rhizosphere of different crops (peas, wheat, and maize) were screened for their growth promoting ability in peas under axenic condition. Three rhizobacterial isolates, Pseudomonas fluorescens biotype G (ACC-5), P. fluorescens (ACC-14), and P. putida biotype A (Q-7), were selected for pot trial on the basis of their source, ACC deaminase activity, root colonization, and growth promoting activity under axenic conditions. Inoculated and uninoculated (control) seeds of pea cultivar 2000 were sown in pots (4 seeds/pot) at different soil moisture levels (25, 50, 75, and 100% of field capacity). Results revealed that decreasing the soil moisture levels from 100 to 25% of field capacity significantly decreased the growth of peas. However, inoculation of peas with rhizobacteria containing ACC deaminase significantly decreased the "drought stress imposed effects" on growth of peas, although with variable efficacy at different moisture levels. At the lowest soil moisture level (25% field capacity), rhizobacterial isolate Pseudomonas fluorescens biotype G (ACC-5) was found to be more promising compared with the other isolates, as it caused maximum increases in fresh weight, dry weight, root length, shoot length, number of leaves per plant, and water use efficiency on fresh and dry weight basis (45, 150, 92, 45, 140, 46, and 147%, respectively) compared with respective uninoculated controls. It is highly likely that rhizobacteria containing ACC deaminase might have decreased the drought-stress induced ethylene in inoculated plants, which resulted in better growth of plants even at low moisture levels. Therefore, inoculation with rhizobacteria containing ACC deaminase could be helpful in eliminating the inhibitory effects of drought stress on the growth of peas.

Isolation of pathogenic Photobacterium damselae subsp. damselae from olive flounder, Paralichthys olivaceus (넙치, Paralichthys olivaceus에서 병원성 Photobacterium damselae subsp. damselae의 분리)

  • Kwon, Mun-Gyeong;Park, Saung-Un;Bang, Jong-Deuk;Park, Soo-Il
    • Journal of fish pathology
    • /
    • v.18 no.3
    • /
    • pp.205-214
    • /
    • 2005
  • The isolates, which has caused considerable damage to the olive flounder farm located in the eastern coast of Korea showed 99% sequence homology in the comparison of 16s rRNA gene of P. damselae subsp. damselae ATCC 33539. The present P. damselae was identical to the biotype No.8 in Pedersen et al. (1997) and the same LPS protein pattern as P. damselac subsp. damselae ATCC 33539. The comparison of infection rates among present P. damselae and Vibrio spp. showed that isolated P. damselae was the highest, followed by V. anguillarium, V. harveyi. and V. ordalii.

A Report on Mixed Occurrence of Tobacco Whitefly (Bemisia tabaci) Biotypes B and Q in Oriental Melon Farms in Kyungpook Province, Korea (담배가루이 생태형 B와 Q가 같이 발생하는 경북 참외재배 지역 보고)

  • Kim, Eunsung;Kim, Yonggyun
    • Korean journal of applied entomology
    • /
    • v.53 no.4
    • /
    • pp.465-472
    • /
    • 2014
  • The tobacco whitefly, Bemisia tabaci, infest the Oriental melon and give significant economic damage along with its virus-vectoring activity. Various biotypes of B. tabaci have been well known and are classified depending on the severity of crop damage and insecticide susceptibility. In this study, B. tabaci adults were collected in the melon fields located in Poongchun-myeon, Andong, Korea and diagnosed on their biotypes using PCR molecular markers. From the all the 11 greenhouses, B. tabaci biotype Q was identified. In addition, biotype B adults were also found from the 4 greenhouses. These results report the first occurrence of B. tabaci at the Oriental melon farms in Gyeongbuk province with mixed infection by the two biotypes in the area.