• Title, Summary, Keyword: bovine brucellosis

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Studies on outbreak of bovine brucellosis in Kyunggi province;I. The epidemiological characters of brucella-reactor cattle and properitis of isolated Brucella abortus (경기도에서 발생하는 유우부루셀라병에 관한 연구;I. 감염우의 역학조사 및 분리균의 특성에 관하여)

  • 심항섭;고태오;유성종;우종태;박병옥;김성렬;박유순
    • Korean Journal of Veterinary Service
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    • v.19 no.3
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    • pp.189-198
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    • 1996
  • The survey was performed to provied details about the pattern of bovine brucellosis occurred in Kyunggi province area. The results obtained through the investigations were summarized as follows. Five hundred seventy - three cattle of bovine brucella reactor were occurred in 14 districts among 31 districts in Kyunggi province in 1989-1995. Among them, 370 cattle(64.5% ) were bred in Eastern area(Ichon, Yeju, Kwangju) and 153 cattle(26.7% ) in Southern area(Yongin, Ansung, Peungtack) And the number of farms occurred by bovine brucellosis was 110 ones. When we investigated the occurrance frequency for the 110 farms, the ratio of farms which was brocken out just one time was 67.3a and more than twice was 32.7%. In 573 cattle, 271 cattle were reoccurred in farms which had broken out the bovine brucellosis more than one time. And this survey said the interval of reoccurrance was like this ; within a month 50.2%, within two month 19.2%, within four month 7.4%, within six month 7.4%, within an year 15.1%, within 2 year 7.0%. Brucella abortus was isolated from 38 cattle of the 61 ones cattles, and in type all isolated belong to biotype 1.

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Evaluation on diagnostic efficiency of the standard tube agglutination test for bovine brucellosis (소 브루셀라병 표준시험관응집반응법 진단효율 평가)

  • Sung, So-Ra;Kim, Ji-Yeon;Her, Moon;Lee, Kichan;Gu, Jeong-Hui;Kang, Sung-Il;Lee, Hyang-Keun;Kim, Suk-Mi;Jung, Suk-Chan
    • Korean Journal of Veterinary Service
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    • v.35 no.4
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    • pp.269-273
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    • 2012
  • A confirmatory serological test, the standard tube agglutination test (STAT) is evaluated for the diagnostic efficiency in brucellosis Korea. A total of 345 bovine samples were collected from regional veterinary branch under national brucellosis monitoring program from January 2010 to June 2012 in Korea. These samples were diagnosed as suspected serum and brucellosis positive by the Rose Bengal test (RBT) and the STAT, respectively. The STAT was compared and evaluated with three serological test such as the indirect-enzyme linked immunosorbent assay (I-ELISA), competitive-enzyme linked immunosorbent assay (C-ELISA) and fluorescence polarisation assay (FPA) prescribed for international trade by OIE. Among the 345 bovine serum samples, 302 (87.5%) were diagnosed as positive in the STAT, while 215 (62.3%), 223 (64.6%) and 194 (56.2%) serum samples were diagnosed as positive for brucellosis in the I-ELISA, C-ELISA and FPA, respectively. The STAT showed quite high positive results as compared with three prescribed tests of OIE. FPA, I-ELISA and C-ELISA have shown 60.6%, 64.9% and 67.2% correlation, respectively as compared to the STAT. However correlations of three prescribed tests ranged high 84.1~97.7%. Especially, correlation between I-ELISA and C-ELISA is quite high, 97.7%. These results suggest that the STAT has shown many false-positive reactions. Therefore, additional serological test, such as ELISAs and FPA, would be necessary to adopt as a confirmatory test in the national surveillance program of bovine brucellosis in Korea.

Studies on incidence of Bovine Brucellosis in Cheju-Do (제주도내 축우 부루셀라병 발생상황 조사)

  • 김우택;이완수;김공식
    • Korean Journal of Veterinary Service
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    • v.14 no.2
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    • pp.104-109
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    • 1991
  • Tile present study was carried out to investigate the incidence of bovine brucellsis in Cheju-do during the period from 1985 to 1990. The results were summarized as follows. 1. In the total 239,238 cattles tested. 1180(0.49%) were positive by standard tube agglutination test during the period from 1985 to 1990. 2. The major causes of incidence on brucellosis was grazing with carriers and repeated incidence in a herd. 3. The 13 Brucella abortus biotype 1 isolated from 10(50%) of 20 cattles slaughtered on brucellosis in 1990.

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Abortion Caused by Bovine Brucellosis in the Chonnam Province (전남 지방의 소에서 브루셀라병에 의한 유산)

  • Oh, Hyun-Yi;Park, Chul-Ho;Kim, Sang-Il;Bae, Jae-Han;Ryu, Jae-Sun;Kim, Bang-Sil;Lee, Ju-Hwan;Kim, Jae-Pung;Park, In-Chul;Kim, Jong-Taek;Suh, Guk-Hyun;Son, Chang-Ho;Oh, Ki-Seok
    • Journal of Embryo Transfer
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    • v.25 no.1
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    • pp.43-50
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    • 2010
  • The specimens from 32 aborted fetus and 274 aborted cows were collected in 168 farms of Chonnam province from 2005 to 2008 and were tested the brucellosis. The results obtained are summarized as follows. In the 32 aborted fetus, bovine brucellosis was detected in 12 heads (37.5%), bovine viral diarrhea-mucosal disease was detected in 7 heads (19%), ainovirus infection was detected in 1 head (3.1%), and multi-infection of BVD and brucellosis was not detected, respectively. In the 306 cases of aborted fetus and cows, bovine brucellosis was detected in 44 heads (14%). Status of abortion were confirmed in 63 farms (38%) out of 168 farms from June to August. From the point of raising scale, studies found that 128 farms (76%) out of all raised under 20 heads. The incidence of abortion by brucellosis was mainly showed in 30 heads (68.1%) about 151~250 days of gestation. In the result of the 18 farms survey, the causes of infections were detected movement of infected cattle in 5 farms (28%), unknown cause in 12 farms (67%), and recurrence in 1 farm (5%). The results of this study suggest to take an advantage of the prevention and fundamental research for bovine brucellosis in Chonnam province.

Comaprison of Six Serological Methods for the Diagnesis of Bovine Brucellosis (브루셀라병(病) 검색(檢索)에 사용(使用)되는 여러가지 혈청진단법(血淸診斷法)의 비교연구(比較硏究))

  • Kim, Keum-Hwa;An, Soo-Hwan;Park, Yong-Ho;Kim, Dong-Sung
    • Korean Journal of Veterinary Research
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    • v.22 no.2
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    • pp.149-153
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    • 1982
  • Results obtained from six secological tests for diagnosing bovine brucellosis-standard plate agglutination test (SPT), standard tube agglutination test(STT), complement fixation test(CFT), Rivanol test (RT), agar gel precipitation test (AGP) and counterimmunoelectrophoresis(CIEP) were compared using 38 sera from brucella reactors and 222 sera from dairy and beef cattle in field. The SPT gave 1.6% apparent false negative reactions and 15.4% apparent false positive reactions when compared with STT which is an official test for bovine brucellosis in this country. The distribution of antibody titers determined by STT showed that 37.5% of 38 reactors had antibody titers ranging from 100 to 200, and the remaining 62.5% had antibody titers of 400 or higher. when 38 reactor judged by STT were tested by CFT and RT, 32 cattle(82.4%) were positive by CFT and 33 cattle (86.8%) were positive by RT, respectively. This results suggest that RT is comparable to CFT in the diagnosis of bovine brucellosis. The results also indicated that both AGP and CIEP were insensitive to detect brucella infection in cattle.

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Comparison of a new ELISA with other serodiagnostic tests for bovine brucellosis (소 브루셀라병의 혈청학적 진단법 비교실험)

  • Hur, Jin;Kakoma, Ibulaimu;Jeong, Jae-Myong;Lee, Hyeon-Jin;Baek, Byeong-Kirl
    • Korean Journal of Veterinary Service
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    • v.30 no.3
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    • pp.385-391
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    • 2007
  • A novel enzyme linked immunosorbent assay (ELISA) is described and compared with other established serologic tests for bovine brucellosis, namely the rose bengal test (RBT), the complement fixation test (CFT), and the tube agglutination test (TAT) approved and used in Korea. A total of 109 bovine serum samples were tested using all the 4 assays and analyzed as to specificity, sensitivity, reproducibility and predictive value. The ELISA showed 100% agreement with the CFT. The least agreement between ELISA was observed with the TAT. The agreement between the ELISA and RBT was not significantly different from that observed between the CFT and the ELISA. It is concluded that the new assay would be a good candidate for routine serologic survey for brucellosis in Korea. A protocol combining the ELISA and the CFT would increase the power for detection of serologically positive individuals and herds.

A comparison of agglutination tests and enzyme-linked immunosorbent assay for the bovine brucellosis (소 브루셀라병 혈청 항체가 응집반응 비교 시험)

  • Kim, Cheol-Ho;Bang, Sang-Young;Jeon, Jae-Hyung;Bhak, Jong-Sik;Lee, Min-Kwon;Shin, Jung-Sub
    • Korean Journal of Veterinary Service
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    • v.31 no.3
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    • pp.315-329
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    • 2008
  • A total of 710 bovine serum samples which are composed 532 bovine serum samples showed negative reaction and 178 bovine serum samples showed positive reaction with tube agglutination test (TAT) from North area of Gyeong-nam, Korea were tested using all the 3 assays which are Rose-Bengal test(RBT), tube agglutination test (TAT) and enzyme-linked immunosorbent assay (ELISA, two types) and analyzed for evaluation of specificity, sensitivity, reproducibility and predictive value. In the comparison of serum antibody titer agglutination test, RBT showed almost agreement with TAT. In the comparison of TAT and two types of ELISA method, they showed difference in specificity and sensitivity about 5%. But there is no significant difference in detecting sensitivity between two types of ELISA method and TAT. In serologic tests for bovine brucellosis, the new assay ELISA would be a good candidate for serologic survey for bovine brucellosis in Korea because it is efficient in detecting many test samples quickly. But the serum agglutination tests (RBT, TAT) are more economical and easy assay for detection. In the test of comparison of antibody titer between first day of finding and 10 days after finding by TAT, there was no change in 55% (76/139) of positive cattle.

Bacteriological detection of Brucella abortus and its characterization by PCR in the sporadic outbreak of bovine brucellosis in Gyeonggi province

  • Yang, Su-Jeong;Shim, Hang-Sub;Woo, Jong-Tae;Kim, Hye-Sung;Lee, Sung-Sik
    • Korean Journal of Veterinary Service
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    • v.30 no.2
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    • pp.251-258
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    • 2007
  • Bovine brucellosis has occurred for years in Gyeonggi province under the national test and slaughter scheme. The serum agglutination test (SAT) is a diagnostic tool to confirm the disease despite the argument on its specificity. We selected 8 farms where only one or two individuals were diagnosed as brucellosis through SAT at the primary regular herd check and isolated the causative organism and characterized the species by species-specific PCR. The pathogen isolation was successful in 6 farms out of 8 farms by microbiological culture, showing the successful rate of 75%. The isolation rate of the causative organism represents 70% from supra-mammary lymph node and 60% from uterine tissues. They were characterized as Brucella abortus biovar 1 after biotyping by PCR, showing the fragment of 498 bp. Five of 8 farms were diagnosed as brucellosis two to four times more over the intervals of two or three months. Here in this study we briefly showed the correlation of the sporadic outbreak of brucellosis tested by SAT and the isolation of the causative organism. Moreover one or two reactors against brucellosis among considerable size of herd may indicate that SAT failed to detect potentially infected individuals in the incubation stage or chronic phase of the disease.

Comparison of process and cost of disposal methods for brucellosis infected bovine carcasses: burial, recycling, and incineration (브루셀라병 감염소 사체처리 방법별 절차 및 소요비용 비교: 매몰, 재활용, 소각)

  • Yoon, Hachung;Yhee, Ji-Young;Yu, Chi-Ho;Kim, Jong-Hyuk;Moon, Oun-Kyong;Park, Jee-Yong;Nam, Gun-Wook;Sur, Jung-Hyang;Rhee, Hae-Chun;Kim, Tae-Jong;Lee, Sang-Jin
    • Korean Journal of Veterinary Research
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    • v.49 no.2
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    • pp.141-147
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    • 2009
  • In Korea, burial is the most common method of disposing animal carcasses culled due to brucellosis infection. However, burial has many disadvantages such as shortage of appropriate burial sites, possibile pollution of ground water supply, and negative view of the public. In this study, we have reviewed 3 legal methods for disposing bovine carcasses, which are burial, incineration, and rendering. We also described the overall process, advantages and disadvantages, and required costs for each method. About 75% of bovine brucellosis outbreak farms had less than 3 reactors, and in our study, rendering required the least amount of cost for farms with a small number of reactors (1-3 heads). Our findings suggest that the use of rendering should be encouraged for farms with bovine brucellosis and other methods considered only if rendering is inappropriate.

Epidemiological characteristics of bovine brucellosis in Korea, 2000~2004

  • Nam, Hyang Mi;Yoon, Hachung;Kim, Cheol-Hee;More, Simon J.;Kim, Seok-Jae;Lee, Byeong-Yong;Park, Choi-Kyu;Jeon, Jong-Min;Wee, Sung-Hwan
    • Korean Journal of Veterinary Research
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    • v.52 no.1
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    • pp.19-24
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    • 2012
  • This paper describes the epidemiological characteristics of bovine brucellosis in Korea during January 2000~September 2004, which encompasses the period when the incidence of bovine brucellosis increased abruptly. Data from the National Animal Infectious Disease Data Management System were used for this study. A range of epidemiological measures was calculated including annual herd and animal incidence. During the study period, there were 1,183 outbreaks on 638 farms. In beef cattle, annual herd incidence increased from 0.2 (2000) to 11.5 (2004, to September) outbreaks per 10,000 and annual animal incidence varied between 3.4 (2000) and 105.8 (2004, to September) per 100,000, respectively. On 401 (62.9%) infected farms during this period, infection was eradicated without recurrence. Recurrence of infection was significantly higher on farms where abortion was reported (53.3%), compared to farms where it was not (30.0%). On beef cattle farms, infection was introduced most frequently through purchased cattle (46.2%). Based on the results of this study, the establishment and spread of brucellosis in the Korean beef cattle population were mainly due to incomplete or inappropriate treatment of aborted materials and the movement of infected cattle.