• Title, Summary, Keyword: bovine mastitis

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Efficacy of the AHI Mastitis Detector for the Diagnosis of Bovine Mastitis (전도율측정법(傳導率測定法)(AHI Mastitis Detector)을 이용(利用)한 유방염진단(乳房炎診斷)의 야외적용시험(野外適用試驗))

  • Park, Yong-Ho;Kim, Keum-Hwa;An, Soo-Hwan;Kim, Dong-Sung
    • Korean Journal of Veterinary Research
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    • v.22 no.2
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    • pp.273-278
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    • 1982
  • A total of 136 dairy cows were subjected to test for bovine mastitis by AHI mastitis detector, microbiological test (MT) and California mastitis test (CMT). The results obtained were summarlized as follows: MT indicated that the most important causative microorganisms isolated from mastitic milk were Staphylococcus aureus (59.3%), Staphylococcus epidermidis (16.4%), Streptococcus agalactiae (12.4%) and Streoticoccus uberis (4.4%). In Holstein breed, the critical threshold of electrical conductivity values of normal and mastitic milk were found to be less than $6,900{\mu}S$ and above, $7,700{\mu}S$, respectively. Although there was goad agreement (92.0%) between AHI mastitis detector test and CMT for the diagnosis of bovine mastitis, the diagnostic efficiency of AHI mastitis detector (80.0%) was higher than that of CMT (74.0%) when compared with microbiological findings. In addition, handiness and objectivity of AHI mastitis detector for the detection of mastitic milk suggested that this could effectively be used for the diagnosis of both clinical and subclinical bovine mastitis in field.

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Susceptibilities of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Milk of Bovine Mastitis to Antibiotics Combined with Sulbactam (젖소 유방염에서 분리된 메티실린 내성 황색포도상구균에 대한 항생제와 Sulbactam 병합의 항균효과)

  • Yoo, Jong-Hyun;Han, Hong-Ryul;Park, Hee-Myung
    • Journal of Veterinary Clinics
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    • v.25 no.4
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    • pp.231-235
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    • 2008
  • Various kinds of antibiotic intramammary infusions are used for treatment of bovine mastitis. As antibiotic-resistant bacteria are increased, the therapeutic rate for bovine mastitis is decreased. The goal of this research is to detect significant synergic effects of combination of antibiotics with sulbactam, $\beta$-lactamase inhibitor, on methicilin-resistant Staphylococcus aureus (MRSA). We used 5 strains of MRSA isolated from bovine mastitis with clinical and subclinical signs. All of the bacteria isolated had resistance to oxacillin and showed multi-resistant patterns in the antimicrobial susceptibility tests. Minimal bactericidal concentrations of ampicillin, amoxicillin, cephalexin, ampicillin/sulbactam(2:1), amoxicillin/sulbactam (2:1), and cephalexin/sulbactam (1:1) were measured according to broth microdilution method suggested by National Committee for Clinical Laboratory Standards (NCCLS, M31-A2) to compare the synergic effects of sulbactam combination with each antibiotic alone. Ampicillin and amoxicillin showed synergic antibacterial activity to 4 and 3 respectively in 5 strains of MRSA in combination with sulbactam. This study demonstrates that ampicillin/sulbactam and amoxicillin/sulbactam can be therapeutic choices for mastitis associated with MRSA.

Epidemiological Study on the Bovine Mastitis in Chonnam Area (乳牛의 乳房炎에 관한 疫學的 연구 -전남지역을 중심으로-)

  • Jung, Hee-Kon
    • Journal of Environmental Health Sciences
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    • v.13 no.2
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    • pp.75-81
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    • 1987
  • Epidemiological study on the bovine mastitis was carried out on 1,493 raw milk samples from 7,406 dairy cows of dairyfarms in Chonnam province. And, it were classified by R. B. V. test during the period from July, 1984 to June, 1986. Of these positive samples, the mastitis causative agents were isolated, and examined for susceptibility to 14 antibiotics by disc method. The results are summarized as follows 1. Incidence rate of bovine mastitis from raw milk examined was observed in 1,393 dairy cows (18.8%) among 7,406 dairy cows of dairy farms. These outbreaks according to years were increased to 17.1% in 1984, 19.7% in 1985, and 20.5% in 1986. And, outbreaks of bovine mastitis according to seasons were high observed during the period from August to October. 2. Isolated rate of mastitis causative agents from raw milk was observed in the order of Staphylococcus sp. (50.2-51.7%), Streptococcus sp. (35.6-48.3%), Bacillus sp. (2.2-6.7%), Pseudomonas sp. (0.3-5.7%), and others (0.6%). 3. Generally, antibiotics susceptibility of isolated mastitis agents from raw milk was observed high susceptible in CP, CL, NB, PC, SM, NM, EM, GM etc.

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Dehydrating and bacterial elimination effects of fecal dehydrating system for reducing bovine mastitis derived from environmental contamination (환경유래 젖소유방염 저감을 위한 우분뇨 탈수 시스템의 탈수 및 유방염 원인체 제균 효과 규명)

  • Kim, Dong Hyeok;Lim, Jung Ju;Lee, Jin Ju;Kim, Dae Geun;Chang, Hong Hee;Lee, Seung Joo;Lee, Yun Beom;Chang, Dong Il;Lee, Hu Jang;Min, Won-Gi;Kim, Sang Hun;Oh, Kwon Young;Kim, Suk
    • Korean Journal of Veterinary Research
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    • v.49 no.3
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    • pp.257-263
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    • 2009
  • Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. Escherichia coli and Staphylococcus aureus are the major causative agents of bovine mastitis. These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Bovine mastitis causative micro-organisms can survive in 1-2 weeks in feces and bed complexes. Low level of percentage of water content (PWC) of feces and bed complexes can reduce the spreading of bovine mastitis incidence from environmental contamination. In this study, we developed the fecal dehydrating system and determined the elimination rates of bovine mastitis causative agent from feces and bed complexes. To develop the fecal dehydrating system, the screw pressurized dehydrating system was used and the maximum rate of dehydrating was reached to 52% PWC using 90% PWC (wet base) of fecal and bed complexes. The elimination rates of the dehydrating system for E. coli and S. aureus were reached at 41.19 $\pm$ 7.84% to 62.55 $\pm$ 8.71% in various percentages of PWC of feces and bed complexes (80, 85 and 90%). These results suggested that the application of fecal dehydrating system would reduce the exposure of dairy cattle to bovine mastitis causing agents contaminated feces and bed complexes, and can be used for environmental bovine mastitis control avoiding misuse or abuse of chemical disinfectants and antibiotics in dairy farm.

Characterization of Bacteriocin produced Lactobacillus bulgaricus acting on bovine mastitis pathogens

  • Kim, Hyun-Jin;Kim, Ji-Hyun;Kim, Sung-Koo
    • 한국생물공학회:학술대회논문집
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    • pp.740-744
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    • 2003
  • The antimicrobial substance produced by Lactobacillus bulgaricus was inactivated by pretense, which confirmed it as a bacteriocin and referred to 'bulgaricin HJ'. The bulgaricin HJ showed the inhibitory activity against mastitis pathogens, gram-positive and gram-negative bacteria. The optimal conditions for the production of bulgaricin HJ were at the temperature of $30^{\circ}C$ and 10 h after cultivation of L. bulgaricus. Staph. and Strep. agalactiae, common bovine mastitis pathogens, were treated with bulgaricin HJ by the agar well diffusion method and showed antimirobial activities to the bovine mastitis pathogens. The activity of the bulgaricin HJ was maintained at pH 6-7 and $100^{\circ}C$ for 60 min against the mastitis pathogens. The bulgaricin HJ was determined as class IV bacteriocin by various enzyme treatments. Colony forming units analysis with indicator strains by the treatments of bulgaricin HJ indicates that the mode of bacteriocin action was bactericidal rather than bacteriostatic.

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Comparison of Natural Resistance-associated Macrophage Protein (NRAMP)1 Expression between Cows with High and Low Milk Somatic Cells Counts

  • Joo, Y.S.;Moon, J.S.;Fox, L.K.;Suh, G.H.;Kwon, N.H.;Kim, S.H.;Park, Y.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.12
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    • pp.1830-1836
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    • 2003
  • Studies using natural resistance-associated macrophage protein (NRAMP) identification indicated that cattle could be selected for immunity. Several studies performed on intracellular organisms such as Mycobacterium, Salmonella, Brucella and Leishmania in human and mouse revealed that resistance against these bacteria was dependent on high activity of NRAMP1 in macrophages. However, hardly any researches have been done on Staphylococcus aureus in bovine mastitis, which is an intracellular organism and the main cause of bovine mastitis. The objectives of this study were to establish reverse transcriptase polymerase chain reaction (RT-PCR) methods, through which NRAMP1 mRNA expression could be compared and analyzed between mastitis-resistant and -susceptible cows. NRAMP1 gene and its expression were investigated using 20 cows (Holstein Friesian) in Korea. Cows were evenly split into two groups, with and without histories of clinical mastitis. Equivalent numbers of cows were randomly selected from each group. Monocytes were isolated from the bovine peripheral blood of each selected cows and activated with lipopolysaccharide (LPS). mRNA was separated from the monocytes and cDNA of NRAMP1 was synthesized and amplified using RT-PCR with amplification of $\beta$-actin as a control. The difference in NRAMP1 expressions of mastitis-resistant (n=10) and -susceptible (n=10) Holstein cows was analyzed. Results demonstrate that resistant cows produced more NRAMP1 mRNA than the susceptible ones, and ratios of NRAMP1:$\beta$-actin expression were higher in resistant cows with or without LPS activation. Therefore, this study could be applied to select bovine mastitis resistant cows before infection based on the expression of NRAMP1.

Diagnostic test for bovine mastitis by the determination of ATP based on firefly bioluminescence (Bioluminescence 반응에 의한 ATP 측정을 이용한 젖소 유방염 진단에 관한 연구)

  • Kim, Tae-jong;Kim, Jong-bae;Lee, Seoung-bae;Jeon, Young-soo
    • Korean Journal of Veterinary Research
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    • v.29 no.3
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    • pp.383-391
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    • 1989
  • This study was carried out to diagnostic test for bovine mastitis by the determination of adenosine triphosphate (ATP) based on firefly bioluminescence. The results obtained are follow; 1. The infection rate of bovine mastitis investigated with 521 cows in 47 dairy farms were found to be 3.6% of clinical form and 44.1% of subclinical form according to the degree of infection. 2. The light yield produced in firefly bioluminescence system was proportional to the concentration of ATP giving stright line within the range of 100PM~1uM. 3. When the number of somatic cell in milk was determined by the ATP assay and compared with three conventional methods such Fossomatic, California mastatic test (CMT), and rolling ball viscometer (RBV), it was shown that r=0.92 for Fossomatic, 0.63 for CMT and 0.7 for RBV. 4. The microorganisms causing mastitis were isolated Staphylococcus sp. (53.3%), Streptococcus sp. (17.9%), Micrococcus sp. (13.5%), Gram negative bacilli (6.3%), Gram positive bacilli (5.5%) and Yeast-like fungi (5.4%). 5. The endogeneous ATP levels of bacteria in a raw milk determined by the firefly bioluminescence system and compared with the results of the conventional methods. The correlation was 0.88 for raw milk.

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A Study on the Effects of Bovine Mastitis Control Using SCC, CMT and Pathogens (SCC, CMT와 세균검사에 의한 유방염의 지도효과 연구)

  • 최진영;황현순;손봉환;김순재
    • Korean Journal of Veterinary Service
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    • v.15 no.2
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    • pp.150-165
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    • 1992
  • The study for a effect of monitoring on bovine mastitis was conduced for a year from Dec, 1989 to Nov, 1990, Sampling the bulk milk of 350 herds in Inchon city and out of 10 herds among them were carried out herds guidance, CMT, SCC, isolation of pathogens and antibiotic sensitivity tests. The results obtained were summarized as follows 1. Annual mean SCC of 1213 herds was 558, 000 cell /ml 2. The number of SCC below 500, 000 cell /ml to quarters for herds guidance was at 1st 77. 0%, End 84.8% and 3rd 80.4%. The is shown that milk quality was steadly improved. 3. The most number of isolated pathogens of bovine mastitis was Staphylococcus SPP - 402(47.2%) Streptococcus SPP - 80(18.7% ) 4. The highest rate of antibiotic sensitivity test was Stapylococcus SPP - cephalothin(76.7%) Streptococcus SPP - ampicillin(77.5%) Gram negative bacilli - tetracyclin(76.0%) 5. The effect of monitoring on bovine mastitis was improved showing that at 1st 49.0% to 3rd 72.0%

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Ecological Studies on Causative Agents of Food Poisoning isolates from Food Animals #2. Time Variation of Bovine Mastitis outbreak in a Municipal Area and Antibiotic Susceptibility of Pathogens (축산 유래 식중독 세균에 관한 생태학적 연구 제2보 일부 도시 근교지역 젖소 유방염의 발생양상과 항생제 감수성의 경시 변화)

  • 정희곤
    • The Korean Journal of Food And Nutrition
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    • v.12 no.1
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    • pp.7-12
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    • 1999
  • Bovine mastitis was examined by use of the rolling ball viscosity test in Kwang-ju area. Antibiotic sus-ceptibility of isolated pathogens from raw milk was investigated by the disc method. Positive rate of so-matic cell counts (over 500,000 cells/ml) was decreased from 11.1% in 1991∼1993 to 8.0-% in 1995∼1997. Isolated mastitis-causing-pathogens were identified as Staphylococcus sp. (49.7%) Escherichia coki(23.2%) Streptococcus sp. (10.4%) and Bacillus sp(7.2%) Antibiotic susceptibility of isolated pathogens to almost antibiotics was decreased.

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