• Title, Summary, Keyword: bursa of Fabricius

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Studies on Histological Changes of Bursa of Fabricius in Chicken Treated with Thyroxine III. Electron Microscopic Observations on Bursa of Fabricius in Chicken Administered with Thyroxine or Propylthiouracil (갑상선(甲狀腺) 호르몬이 닭의 Fabricius 낭(囊)에 미치는 조직학적변화(組織學的變化)에 관(關)한 연구(硏究) III. Thyroxine 및 Propylthiouracil을 투여(投與)한 닭의 Fabricius 낭세포(囊細胞)에 대(對)한 전자현미경적관찰(電子顯微鏡的觀察))

  • Kim, Soon Bok;Lee, Cha Soo
    • Korean Journal of Veterinary Research
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    • v.20 no.2
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    • pp.105-112
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    • 1980
  • The bursa of Fabricius from thyroxine-treated group showed increased number of large lymphocytes with well-developed cytoplasm, immunoblasts and proplasmacytes accompanied with active cell divisions. More prominent development of Golgi apparatus and mitochondria in the cytoplasm of the cells in the bursa from this group were also observed. The bursa of Fabricius from the propylthiouracil-treated group, however, distinctively revealed proliferation of fibroblasts in the interstitial tissues, decreased number of lymphocytes, and necrotic lymphocytes in the follicles.

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EFFECT OF THE BURSA OF FABRICIUS OF CHICKEN ON ANTIBODY FORMATION AGAINST NEWCASTLE DISEASE VIRUS(B1 Strain) (Newcastle Virus (B1 Strain)에 대(對)한 항체형성(抗體形成)에 미치는 닭의 Fabricius' Bursa의 영향(影響))

  • Cho, Byung Ryul;Cheong, Chang Kook
    • Korean Journal of Veterinary Research
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    • v.2 no.1
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    • pp.51-54
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    • 1962
  • Chickens of Leghorn breed were bursectomized at 3 weeks of age and inoculated intranasally at 4 weeks of age with the $B_1$ strain of NDV. There was no statistically significant difference(P>0.1) in the serum titers (HI test) between the bursectomized chickens and the control chickens.

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Histological and immunohistochemical studies on the endocrine cells in the bursa of Fabricius of duck, Anas platyrhynchos platyrhyncos Linne (청둥오리 Fabricius 낭에 대한 조직학적 및 면역조직화학적 연구)

  • Lee, Jae-hyun;Ku, Sae-kwang;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.38 no.3
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    • pp.461-466
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    • 1998
  • The histological profiles and endocrine cells in the bursa of Fabricius of the duck (Anas platyrhynchos platyrhyncos, Linne) were studied at 23 days of incubation, at hatching, 1 week, 2 weeks, 3 weeks, 5 weeks, 6 weeks, 7 weeks, 9 weeks, 10 weeks and 32 weeks after hatching. Histologically epithelium of bursa Fabricius were pseudostratified columnar and simple columnar epithelium. Numerous lymphatic follicles and well developed plica were observed in this study. The sections were reacted immunohistochemically using antisera specific to bovine chromogranin(BCG), serotonin and somatostatin antisera using immunohistochemical methods. A few number of BCG-immunoreactive cells were detected from 23 days of incubation to 5 weeks after hatching and serotonin-immunoreactive cells were also observed from 23 days of incubation to 3 weeks after hatching. No somatostatin-immunoreactive cells were detected in this study.

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Studies on Histological Changes of Bursa of Fabricius in Chicken Treated with Thyroxine; I. Light Microscopic Observations on Bursa of Fabricius Including Other Organs in Chicken Administrated with Thyroxine or PropylthiouraciI (갑상선(甲狀腺) 호르몬이 닭의 Fabricius 낭(囊)에 미치는 조직학적변화(組織學的變化)에 관(關)한 연구(硏究) I. Thyroxine 및 Propylthiouracil을 투여(投與)한 닭의 Fabricius낭(囊) 및 기타(其他) 장기(臟器) 대(對)한 광학현미경적관찰(光學顯微鏡的觀察))

  • Kim, Soon Bok;Lee, Cha Soo
    • Korean Journal of Veterinary Research
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    • v.20 no.1
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    • pp.17-24
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    • 1980
  • In order to know the effects of thyroid hormone on the bursa of Fabricius in chicken, the bursae were studied with the light microscope after administration of thyroxine(TX) or propylthiouracil(PPT). Macroscopically, the bursa of TY-treated group showed increase in size and thickened folds. while those of the PPT-treated group decrease in size, compared with those of control group. In the light microscopic studies, the bursa of Fabricius of the TX-treated group showed active cell-divisions in the medulla, and increased number of pyroninophilic large lymphocytes and plasma cells containing PAS positive materials in the cytoplasms. On the other hand, the bursa from PPT-treated group revealed decreased number of lymphocytes, significant increase of necrotic lymphocytes in the follicles, and the proliferation of the interfollicular connective tissues. A large number of pyroninophilic lymphocytes and plasma cells were also appeared in the spleen of the TX-treated group.

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Cryptosporidiosis in Chickens (닭의 Cryptosporidiosis 발생례(發生例) 보고(報告))

  • Mo, In-pil;Youn, Hee-jeong;Choi, Sang-ho;Rhee, Young-ok;Namgoong, Sun
    • Korean Journal of Veterinary Research
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    • v.28 no.1
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    • pp.175-177
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    • 1988
  • Light-microscope studies of the coccidial organisms found in the bursa of Fabricius from chickens shelwng some specific signs were carried out, and the etiological agent was estimated as the parasite belonging to the Family Cryptosporiidae. The specific gross Pathological signs were intestinal hemorrhage and atrophy of the bursa of Fabricius and hyperplasia was associated with the presence of organisms attached to the epithelial cells lining the plicae of the bursa of Fabricius in the microscopical findings.

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Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duck

  • Luo, Jun;Liu, Junying;Liu, Hehe;Zhang, Tao;Wang, Jiwen;He, Hua;Han, Chunchun
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.7
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    • pp.920-929
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    • 2017
  • Objective: The bursa of Fabricius (BF) is a central humoral immune organ belonging specifically to avians. Recent studies had suggested that miRNAs were active regulators involved in the immune processes. This study was to investigate the possible differences of the BF at miRNA level between two genetically disparate duck breeds. Methods: Using Illumina next-generation sequencing, the miRNAs libraries of ducks were established. Results: The results showed that there were 66 differentially expressed miRNAs and 28 novel miRNAs in bursa. A set of abundant miRNAs (i.e., let-7, miR-146a-5p, miR-21-5p, miR-17~92) which are involved in immunity and disease were detected and the predicted target genes of the novel miRNAs were associated with duck high anti-adversity ability. By gene ontology analysis and enriching KEGG pathway, the targets of differential expressed miRNAs were mainly involved in immunity and disease, supporting that there were differences in the BF immune functions between the two duck breeds. In addition, the metabolic pathway had the maximum enriched target genes and some enriched pathways that were related to cell cycle, protein synthesis, cell proliferation and apoptosis. It indicted that the difference of metabolism may be one of the reasons leading the immune difference between the BF of two duck breeds. Conclusion: This data lists the main differences in the BF at miRNAs level between two genetically disparate duck breeds and lays a foundation to carry out molecular assisted breeding of poultry in the future.

Effect of Diclazuril on the Bursa of Fabricius Morphology and SIgA Expression in Chickens Infected with Eimeria tenella

  • Zhou, Bian-hua;Liu, Li-li;Liu, Jeffrey;Yuan, Fu-wei;Tian, Er-jie;Wang, Hong-wei
    • The Korean Journal of Parasitology
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    • v.53 no.6
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    • pp.675-682
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    • 2015
  • The effects of diclazuril on the bursa of Fabricius (BF) structure and secretory IgA (SIgA) expression in chickens infected with Eimeria tenella were examined. The morphology of the BF was observed by hematoxylin and eosin staining, while ultrastructural changes were monitored by transmission electron microscopy. E. tenella infection caused the BF cell volumes to decrease, irregularly arranged, as well as, enlargement of the intercellular space. Diclazuril treatment alleviated the physical signs of damages associated with E. tenella infection. The SIgA expression in BF was analyzed by immunohistochemistry technique. The SIgA expression increased significantly by 350.4% (P<0.01) after E. tenella infection compared to the normal control group. With the treatment of diclazuril, the SIgA was relatively fewer in the cortex, and the expression level was significantly decreased by 46.7% (P<0.01) compared with the infected and untreated group. In conclusion, E. tenella infection in chickens induced obvious harmful changes in BF morphological structure and stimulated the expression of SIgA in the BF. Diclazuril treatment effectively alleviated the morphological changes. This result demonstrates a method to develop an immunological strategy in coccidiosis control.

Acute phase protein mRNA expressions and enhancement of antioxidant defense system in Black-meated Silkie Fowls supplemented with clove (Eugenia caryophyllus) extracts under the influence of chronic heat stress

  • Bello, Alhassan Usman;Sulaiman, Jelilat Aderonke;Aliyu, Madagu Samaila
    • Journal of Animal Science and Technology
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    • v.58 no.11
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    • pp.39.1-39.12
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    • 2016
  • Background: The current study investigates the anti-stress effects of clove (Eugenia caryophyllus) extracts (0, 200, 400, and 600 mg/kg) on serum antioxidant biomarkers, immune response, immunological organ growth index, and expression levels of acute phase proteins (APPs); ovotransferrin (OVT), ceruloplasmin (CP), ceruloplasmin (AGP), C-reactive protein (CRP), and serum amyloid-A (SAA) mRNA in the immunological organs of 63-d-old male black-meated Silkie fowls subjected to 21 d chronic heat stress at $35{\pm}2^{\circ}C$. Results: The results demonstrated that clove extract supplementation in the diet of Silkie fowls subjected to elevated temperature (ET) improve growth performance, immune responses, and suppressed the activities of glutathion peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), and thioredoxin reductase (TXNRD); reduced serum malonaldehyde (MDA) and glutathione (GSH) concentrations when compared with fowls raised under thermoneutral condition (TC). Upon chronic heat stress and supplementation of clove extracts, the Silkie fowls showed a linear increase in GSH-Px, SOD, CAT, and TXNRD activities (P = 0.01) compared with fowls fed diets without clove extract. ET decreased (P < 0.05) the growth index of the liver, spleen, bursa of Fabricius and thymus. However, the growth index of the liver, spleen, bursa of Fabricius and thymus increased significantly (P < 0.05) which corresponded to an increase in clove supplemented levels. The expression of OVT, CP, AGP, CRP, and SAA mRNA in the liver, spleen, bursa of Fabricius and thymus were elevated (P < 0.01) by ET compared with those maintained at TC. Nevertheless, clove mitigates heat stress-induced overexpression of OVT, CP, AGP, CRP and SAA mRNA in the immune organs of fowls fed 400 mg clove/kg compared to other groups. Conclusions: The results showed that clove extracts supplementation decreased oxidative stress in the heat-stressed black-meated fowls by alleviating negative effects of heat stress via improvement in growth performance, antioxidant defense mechanisms, immunity, and regulate the expression of acute phase genes in the liver and immunological organs.

Apoptosis of bursal lymphoid cells in chickens experimentally infected with IBDV(SH/92)

  • Lee, Hee-Ryung;Lim, Chae-Woong;Mo, In-Pil;Moon, Hyung-Bae;Kim, Ki-Suck;Rim, Byung-Moo
    • Korean Journal of Veterinary Pathology
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    • v.2 no.1
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    • pp.1-8
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    • 1998
  • Chickens at 3-weeks of age were inoculated with a highly virulent strain (SH/92) of Infectious Bursal Disease Virus(IBDV) through ocular and cloacal routes. The infected chickens were killed at 6, 12, 24, 48, 72, and 96 hrs post inoculation (PI) and Bursa of Fabricius(BF) were collected. The sizes of bursal follicules in infected chickens decreased at 48 to 96 hrs PI. Histologically the cellular changes were first evident at 12 hrs PI and characterized by condensation of nuclear chromatin of bursal lymphocytes indicating apoptosis. By 24 hrs PI apoptotic lymphocytes dramatically increased. In addition infiltration of heterophils were also seen in the follicles and in the interfollicular connective tissues. At 48 hrs PI, cystic cavities were observed in the follicles. As the infection advanced the bursal follicles showed atrophy accompanied by disappearance of heterophils and reduction in number of lymphocytes in the cystic cavities which was replaced by proteineous materials. The nuclei of most affected lymphocyte stained positively with the in situ end labeling for apoptosis. Electron microscopy showed viral particles with crystalline array in the lymphocytes of BF infected with IBOV. These results indicated that SH/92 IBDV infection in chickens caused increased apoptosis in the BF.

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