• Title, Summary, Keyword: carrageenan-induced paw edema

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Effect of nifedipine against carrageenan-induced paw oedema in rats (흰쥐에서 carrageenan에 의한 족부종(足浮腫)에 대한 nifedipine의 영향)

  • Shin, Dong-ho
    • Korean Journal of Veterinary Research
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    • v.30 no.3
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    • pp.283-286
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    • 1990
  • The effects of nifedipne and verapamil were compared on carrageenan-induced paw edema in rats and the results are summarized as follows: Carrageenan induced severe acute paw edema within 30 minutes and the maximum effect was achieved around 4 hours after administration. The carrageenan-induced paw edema was prominantly reduced by pretreatments of indomethacin (2mg/kg, p.o.) and nifedipine (10 and 20mg/kg, i.p.), whereas verapamil had no effect on the carrageenan-induced paw edema. These results suggest that calcium antagonists, nifedipine and verapamil, have a different effect on the inflammatory response induced by carrageenan.

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Anti-inflammaory effects of the MeOH extract of Humulus japonicus in vitro (율초(葎草) 추출물이 Rat의 paw edema에 미치는 영향)

  • Hwang, Sun-Yi;Jung, Ho-Jun;Jang, Woo-Seok;Jo, Mi-Jeong;Kim, Sang-Chan;Jee, Seon-Young
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.22 no.3
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    • pp.71-79
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    • 2009
  • Objectives : The present study was examined to evaluate the anti-inflammatory effects of the Humulus japonicus MeOH extracts (HJE) in the carrageenan-induced paw edema model in rats. Methods : The effects of HJE on anti-inflammation were measured in the carrageenan-induced paw edema model in rats and infiltrated Inflammatory Cells. Results : 1. HJE (1.0 g/kg) and dexamethasone effectively inhibited paw edema measured 1~4 h after carrageenan injection. HJE (0.3 g/kg) effectively inhibited paw edema measured 1, 3, 4 hr. 2. In histopathological study in rats, 1.0 g/kg HJE and dexamethasone effectively inhibited the increases of hind paw skin thicknesses and inflammatory cell infiltrations induced by carrageenan treatment. But quite similar histopathological changes were detected in 0.3 g/kg HJE treated group as compared with carrageenan control.

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Analgesic and anti-inflammatory effect of the aqueous extract of Angelica dahurica

  • Choi, In-Ho;Song, Yun-Kyung;Lim, Hyung-Ho
    • The Journal of Korean Medicine
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    • v.29 no.2
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    • pp.32-40
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    • 2008
  • Background: Angelica dahurica has been used in various clinical cases. Its taste is hot and its property is warm, dry and nonpoisonous. Its efficacy is to remove wind-damp, cure swelling and edema, exhaust pus, stop itching, rhinitis and leukorrhea. Object: To test through experiment Angelica dahurica's analgesic and anti-inflammatory efficacy. Method: Inject acetic acid as a pain-inducing substance to the mice and measure visceral pain bywrithing reflex. Inject carrageenan that is an edema-inducing substance to the rat's paw and measure volume of edema. Take thermal pain to mice with plantar test and measure paw withdrawal latency. Normal group is non Angelica dahurica-treated group and treated group is Angelica dahurica-treated group. Results: In acetic acid-induced visceral model, treatment with Angelica dahurica suppressed writhing reflex significantlyand dose-dependently. In carrageenan-induced paw edema model, treatment with Angelica dahurica suppressed carrageenan-induced paw edema. In plantar test model, no significant effect on the withdrawal latency of thermal stimulation-induced nociception was observed. Conclusion: Angelica dahurica has analgesic and anti-inflammatory efficacy.

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The protective mechanism of melatonin on carrageenan-induced paw edema generation in rats

  • Min, Young-Sil;Yun, Kang-Hee;Ju, Song-Hyun;Ryu, Jung-Su;Sohn, Uy-Dong
    • Proceedings of the PSK Conference
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    • pp.76.1-76
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    • 2003
  • The influence of melatonin on carrageenan-induced paw edema in Sprague-Dawley rats has been studied. The injection of 1% carrageenan (given at 0.1ml/paw) into the intraplantar induced an inflammatory response, and the maximal increase of paw volume, edema, was observed at 4hour. The levels of nitric oxide (NO), malondialdehyde (MDA) or prostaglandin E$_2$ (PGE$_2$) were increased after edema generation. Also, the expression of the inducible NO synthase (iNOS) were increased in the western blot. (omitted)

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Effects of Achyrantes japonica on Carrageenan-Induced Arthritis Rat Model (Carrageenan으로 유발한 관절염 쥐에서의 우슬추출물 효과)

  • Kim, Young-Ock;Lee, San-Won;Lee, Seung-Eun
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.6
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    • pp.470-474
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    • 2009
  • Achyrantes japonica (AJ) has been used to treat edema and arthritis in the traditional Korean medicine. To elucidate the anti-inflammatory and anti-nociceptive effects of ethanol extract of AJ, the carrageenan-induced paw edema using a plethysmometer and thermal hypersensitivity using the plantar test were measured. Ibuprofen was used as a control drug. Treatment with AJ (200mg/kg p.o.) significantly reduced paw edema, compared to the carrageenan - treated rats. In the plantar test, the thermal withdrawal latency in AJ - treated group was significantly increased than the carrageenan - treated group. The results indicate that AJ could have be the anti-inflammatory and anti-nociceptive properties.

Paw Edema was Reduced in Carrageenan Induced Acute Inflammation in Stat4 Deficient Mice

  • Zheng, Long-Tai;Baik, Haing-Woon;Lee, Seong-Kyu;Cho, Jeong-Je;Park, Cheung-Seog;Hong, Mee-Suk;Chung, Joo-Ho;Yim, Sung-Vin
    • Molecular & Cellular Toxicology
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    • v.2 no.4
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    • pp.257-261
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    • 2006
  • Signal transducer and activator of transcription 4 (STAT4) is one of the important mediators in generating inflammation and immune responses. To address the role of Stat4 in carrageenan induced acute inflammation, we performed paw edema measurement and 7.4 k mouse cDNA microarray analysis in carrageenan induced acute inflammation in Stat4 knockout (-/-) mice. Male BALB/c (n=8) and Stat4 -/- (n=5) were used and paw edema was induced with injection of $30\;{\mu}L$ of 1% carrageenan into plantar surface of right hind paw. Next, we isolated the mRNA in mouse whole brain and analyzed cDNA microarray profiles for the changes of the brain expression in Stat4 -/- mice. Interestingly, the increase in paw volume of Stat4 -/- mice was reduced by about 30% as compared to that of wild type. The cDNA microarray analysis revealed the altered expressions of several cytokines (Tnf, Il6, and Il4) and pain-associated proteins (Ptgs2, Gabra6, and Gabbr1) in Stat4 -/- mice. Our results suggest that Stat4 may be related to the inhibitory responses on carrageenan induced acute inflammation.

Analgesic and anti-inflammatory activity of a polyherbal formulation (PHFAROGH)

  • Mohan, M;Gulecha, VS;Aurangabadkar, VM;Balaraman, R;Austin, A;Thirugnanasampathan, S
    • Oriental Pharmacy and Experimental Medicine
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    • v.9 no.3
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    • pp.232-237
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    • 2009
  • The effect of arogh, a polyherbal formulation-PHF [each 3 g powder contained Nelumbo nucifera G. (0.24 g), Hemidesmus indicus R. (0.24 g), Zingiber officinale R. (0.24 g), Terminalia chebula R. (0.24 g), Quercus infectoria O. (0.12 g), Hibiscus rosa-sinensis L. (0.24 g), Rosa damascene M.(0.24 g), Eclipta alba H.(0.24 g), Glycyrrhiza glabra L. (0.24 g)] was investigated in various experimental models of pain and inflammation. Analgesic activity of PHF was studied in mice using acetic acid induced writhing, tail immersion and hot plate methods. Anti-inflammatory activity of PHF was studied in rats using carrageenan induced hind paw edema and formalin induced rat paw edema methods. PHF significantly (P < 0.05) reduced the number of writhings, increased latency to flick tail in tail immersion method and elevated the mean basal reaction time in hot plate method. PHF significantly (P < 0.05) inhibited carrageenan induced hind paw edema and formalin induced rat paw edema. The PHF was tested at dose of 30, 100, 300 and 500 mg/kg.

Anti-Inflammatory Effects of 15Hz Electroacupuncture on the Carrageenan-Injected Rat (Carrageenan 유발염증에 대한 15Hz 전침의 효과에 대한 연구)

  • Han, You-jin;Lee, Yong-tae;Jang, Kyung-jeon
    • Journal of Acupuncture Research
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    • v.20 no.3
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    • pp.166-176
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    • 2003
  • Objective : The aim of this study is to investigate the anti-inflammatory effects of 15Hz electroacupuncture(EA) on carrageenan-injected rats. Inflammation was induced by an intraplantar injection of 1% carrageenan into the right hind paw. Methods : Bilateral EA stimulation with 15 Hz were delivered at those acupoints corresponding to Zusanli and Sanyinjiao in man via the needles for a total of 30 min duration in carrageenan-injected rats. Results: The developing edema was measured 30 minutes interval afer carrageenan injection and 15 Hz EA stimulation presented significant edema inhibition. Three hours after carrageenan injection, prostaglandin $E_2(PGE_2)$ and nitric oxide(NO) levels were measured. The 15Hz EA stimulation significantly inhibited $PGE_2$ and NO production in the right paw. The pro-inflammatory mRNA expression such as cyclooxygenases(COX)-2 and interleukin(IL)-$1{\beta}$ were slightly down-regulated by EA stimulation. The number of COX-2, IL-$1{\beta}$, tumor necrosis factor-${\alpha}$ immunoreactive cells were abundantly observed in paw edema. But these cells were decreased in nmber according to anti-edema effect of 15Hz EA. Conclusions: These results indicate that 15Hz EA stimulation have an alleviation action against carrageenan-induced edema and local inflammation.

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Anti-inflammatory and anti-nociceptive effects of extract of Astragalus membranaceous

  • Kim, Young-Ock;Kim, Su-Kang;Cho, Ah-Rang;Chung, Joo-Ho
    • Oriental Pharmacy and Experimental Medicine
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    • v.7 no.5
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    • pp.569-572
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    • 2008
  • The root of Astragalus membranaceous (AM) has been used to treat edema and arthritis in the traditional Korean medicine. To elucidate the anti-inflammatory and anti-nociceptive effects of ethanol extract of AM, the carrageenan-induced paw edema using a plethysmometer and thermal hypersensitivity using the plantar test were measured. Ibuprofen was used as a control drug. Pretreatment with AM (400 mg/kg p.o.) significantly reduced paw edema, compared to the carrageenan-treated rats. In the plantar test, the thermal withdrawal latency in AM-treated group was significantly increased than the carrageenan-treated group. The results indicate that AM could have be the anti-inflammatory and anti-nociceptive properties.

Antiinflammatory Activity of Flubiprofen Gel on Carrageenan-induced Edema (카리기난 유발 족부종법에 이용한 플루르비프로펜 겔의 항염증작용 평가)

  • Gil, Hyung-Jun;Lee, Jang-Won;Chi, Sang-Cheol
    • YAKHAK HOEJI
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    • v.38 no.5
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    • pp.525-529
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    • 1994
  • Antiinflammatory activity of an 1% flurbiprofen transdermal gel was evaluated using the carrageenan-Induced rat paw edema method. The application of 50 mg of the gel on the rat hind paw, at various time intervals from 0 to 24 hrs before the carrageenan injection, significantly inhibited edema formation in all groups of dosed rats, indicating that the antiinflammatory activitv was maintained up to 24 hrs after the transdermal application of the 1% flurbiprofen gel. The topical $ED_{50}$ of flurbiprofen in the gel was 1.0 mg/kg, whereas the oral $ED_{50}$ of the drug in a suspension was 1.7 mg/kg.

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