• Title, Summary, Keyword: cellular immune response

Search Result 305, Processing Time 0.044 seconds

Effect Inosiplex on Cellular and Humoral Immune Response (Inosiplex가 세포성(細胞性) 및 체액성면역반응(體液性免疫反應)에 미치는 영향(影響))

  • Ha, Tai-You;Lee, Hern-Ku
    • The Journal of the Korean Society for Microbiology
    • /
    • v.16 no.1
    • /
    • pp.57-64
    • /
    • 1981
  • The clinical need for agents to modify immune response in the treatment of viral infection has lead to an increased interest in cellular and biochemical mechanisms regulating the immune response and to the development of a variety of biological and chemical substance with immunomodulatory activity. Inosiplex has shown antiviral activity in tissue culture, animal models and huamn studies through augmentation of immune response. However, the effect of inosiplex on immune response in animal has not been extensively analyzed, and the effect of inosiplex on immune response has been paradoxical depending on the time of administration of inosiplex in relation to that of antigen. Therefore, this study was undertaken to assess the effect of inosiplex on the immune response to sheep red blood cells(SRBC) in normal and viral infected mice. Inosiplex increased cellular immune response and plaque forming lymphocyte response to SRBC, decreased the recovery of S. typhimurium from infected mice spleen, and restored the depressed cellular immune response by measle and newcastle disease virus infections. All of the above results were observed only when inosiplex was given after immunization but did not when given before immunization. These results indicate that inosiplex stimulate the efferent are of immune response and may even block the afferent are, and suggest that inosiplex is a very promising drug in therapy of many viral infections.

  • PDF

Effect of Captafol on the Immune Response in Mice (Captafol이 mouse의 면역반응에 미치는 영향)

  • 박귀례;홍사욱;정규혁;안영근
    • Environmental Analysis Health and Toxicology
    • /
    • v.3 no.1_2
    • /
    • pp.1-8
    • /
    • 1988
  • The effect of captafol on the immunity and also the influence of ethanol to this immune response treated with captafol were investigated in two experimental groups of mice, that one was treated with captafol and the other was treated with captafol and ethanol. The weight of spleen and thymus were reduced by treatment of captafol and the HY titer. HA titer and Arthus reaction were also supressed in both of two treated groups, it showed that the captafol exerts depressive effect on humoral immune response in mice. The DTH and RFC were also impaired in captafol treated mice, so that the captafol exerted effect on the cellular immune response. According to this experiment immunity, the ethanol had influence on immune response by the treatment of captafol. Therefore the ethanol accelated the supression of humoral and cellular immune response.

  • PDF

The Effect of Cimetidine, Ranitidine and Famotidine on the Immune Response in ICR Mice (마우스에 있어서 Cimetidine, Ranitidine 및 Famotidine이 면역반응에 미치는 영향)

  • 안영근;김정훈;이상근
    • Environmental Analysis Health and Toxicology
    • /
    • v.5 no.3_4
    • /
    • pp.37-45
    • /
    • 1990
  • Experiments were performed on mice to investigate the influences of cimetidine, ranitidine and famotidine on the immune response. Immune response were evaluated by antibody, Arthus reaction (Arthus), delayed type hypersensitivity (DTH), rosette forming cell (RFC), phagocyte activity and whit( blood cell (WBC) in mice, sensitized and challenged with sheep red blood cells (SRBC). The weight of liver, spleen and thymus were measured. Following results obtained in this experiment. 1) The administration of cimetidine as compared to normal group significantly decreased Arthus, Hemagglutinin titer (HA), RFC, DTH, WBC and phagocyte activity, but increased the activity of serum albumin. 2) The administration of ranitidine as compared to normal group decreased RFC and HA. 3) The administration of Famotidine as compared to normal group decreased DTH and RFC, and significantly decreased HA, Arthus and serum protein. 4) The administration of ranitidine and famotidine decreased more humoral immune response than cellular immune response, but the administration of cimetidine significantly decreased humoral and cellular immune response, WBC and phagocyte activity.

  • PDF

The Role of Nrf2 in Cellular Innate Immune Response to Inflammatory Injury

  • Kim, Ji-Young;Surh, Young-Joon
    • Toxicological Research
    • /
    • v.25 no.4
    • /
    • pp.159-173
    • /
    • 2009
  • Nuclear factor erythroid derived 2-related factor-2 (Nrf2) is a master transcription regulator of antioxidant and cytoprotective proteins that mediate cellular defense against oxidative and inflammatory stresses. Disruption of cellular stress response by Nrf2 deficiency causes enhanced susceptibility to infection and related inflammatory diseases as a consequence of exacerbated immune-mediated hypersensitivity and autoimmunity. The cellular defense capacity potentiated by Nrf2 activation appears to balance the population of $CD4^+$ and $CD8^+$ of lymph node cells for proper innate immune responses. Nrf2 can negatively regulate the activation of pro-inflammatory signaling molecules such as p38 MAPK, NF-${\kappa}B$, and AP-1. Nrf2 subsequently functions to inhibit the production of pro-inflammatory mediators including cytokines, chemokines, cell adhesion molecules, matrix metalloproteinases, COX-2 and iNOS. Although not clearly elucidated, the antioxidative function of genes targeted by Nrf2 may cooperatively regulate the innate immune response and also repress the expression of pro-inflammatory mediators.

Effects of Squalene on the Immune Responses in Mice(II):Cellular and Non-specific Immune Response and Antitumor Activity of Squalene

  • Ahn, Young-Keun;Kim, Joung-Hoon
    • Archives of Pharmacal Research
    • /
    • v.15 no.1
    • /
    • pp.20-29
    • /
    • 1992
  • Effects of squalene on cellular and non-specific immune responses and antitumor activity in mice were investigated. Cellular and non-specific immunological assay parameters adopted in the present study were delayed-type hypersensitivity reaction and resette forming cells (RFC) for cellular immunity, activities of natural killer (NK) cells and phagocyte for non-specific immunity. Squalene resulted in marked increases of cellular and non-specific immune functions and enhancement of host resistance to tumor challenge in dose-dependent manner.

  • PDF

Epstein-Barr Virus-Associated Gastric Carcinoma and Specific Features of the Accompanying Immune Response

  • Cho, Junhun;Kang, Myung-Soo;Kim, Kyoung-Mee
    • Journal of Gastric Cancer
    • /
    • v.16 no.1
    • /
    • pp.1-7
    • /
    • 2016
  • Epstein-Barr virus-associated gastric carcinoma (EBVaGC) is one of the four subtypes of gastric carcinoma (GC), as defined by the novel classification recently proposed by The Cancer Genome Atlas. EBVaGC has several clinicopathological features such as longer survival and higher frequency of lymphoepithelioma-like carcinoma (LELC) and carcinoma with Crohn's disease-like lymphoid reaction that distinguish it from EBV-negative GC. The intensity and pattern of host cellular immune response in GC have been found to significantly correlate with the prognosis of patients with GC, suggesting that immune reaction and tumor microenvironment have critical roles in the progression of GC, and in particular, EBVaGC. Here, we reviewed the cellular and molecular mechanisms underlying prominent immune reactions in patients with EBVaGC. In EBVaGC, deregulation of the expression of immune response-related genes promotes marked intra-or peritumoral immune cell infiltration. The expression of programmed death receptor-ligand 1 is known to be increased in EBVaGC, and therefore, it has been proposed as a favorable prognostic factor for patients with EBVaGC, albeit some data supporting this claim are controversial. Overall, the underlying mechanisms and clinical significance of the host cellular immune response in patients with EBVaGC have not been thoroughly elucidated. Therefore, further research is necessary to better understand the role of tumor microenvironment in EBVaGC.

Innate Immune Response of NNV Infection in Fish and Its Disease Prevention

  • Lu, Ming-Wei;Wu, Jen-Leih
    • Journal of Marine Bioscience and Biotechnology
    • /
    • v.2 no.3
    • /
    • pp.127-132
    • /
    • 2007
  • The innate immune response which is seen as the initial defense mechanism induced upon foreign invasion has been well documented in higher vertebrates. This has also been observed in fish infected with NNV. However, the fish immune system based on fully established genome project has not been fully elucidated. Therefore, in this review, we hope to correlate NNV infection in fish that has devastated the aquaculture industry, to its host immune system. Further, we discuss the potential preventive measures in overcoming the widespread of this neurodisease.

  • PDF

Analysis of Immune Responses Against Nucleocapsid Protein of the Hantaan Virus Elicited by Virus Infection or DNA Vaccination

  • Woo Gyu-Jin;Chun Eun-Young;Kim Keun Hee;Kim Wankee
    • Journal of Microbiology
    • /
    • v.43 no.6
    • /
    • pp.537-545
    • /
    • 2005
  • Even though neutralizing antibodies against the Hantaan virus (HTNV) has been proven to be critical against viral infections, the cellular immune responses to HTNV are also assumed to be important for viral clearance. In this report, we have examined the cellular and humoral immune responses against the HTNV nucleocapsid protein (NP) elicited by virus infection or DNA vaccination. To examine the cellular immune response against HTNV NP, we used $H-2K^b$ restricted T-cell epitopes of NP. The NP-specific $CD8^+$ T cell response was analyzed using a $^{51}Cr-release$ assay, intracellular cytokine staining assay, enzyme-linked immunospot assay and tetramer binding assay in C57BL/6 mice infected with HTNV. Using these methods, we found that HTNV infection elicited a strong NP-specific $CD8^+$ T cell response at eight days after infection. We also found that several different methods to check the NP-specific $CD8^+$ T cell response showed a very high correlation among analysis. In the case of DNA vaccination by plasmid encoding nucleocapsid gene, the NP-specific antibody response was elicited $2\~4$ weeks after immunization and maximized at $6\~8$ weeks. NP-specific $CD8^+$ T cell response reached its peak 3 weeks after immunization. In a challenge test with the recombinant vaccinia virus expressing NP (rVV-HTNV-N), the rVV-HTNV-N titers in DNA vaccinated mice were decreased about 100-fold compared to the negative control mice.

Identification of Immune Responsive Genes on Benzene, Toluene and o-Xylene in Jurkat Cells Using 35 k Human Oligomicroarray

  • Sarma, Sailendra Nath;Kim, Youn-Jung;Jeon, Hee-Kyung;Ryu, Jae-Chun
    • Molecular & Cellular Toxicology
    • /
    • v.2 no.4
    • /
    • pp.229-235
    • /
    • 2006
  • Volatile organic compounds (VOCs) are a major component of urban air pollution. It is documented that low exposure levels of VOCs induce alterations in immune reactivity resulting in a subsequent higher risk for the development of allergic reactivity and asthma. Despite these facts, there are few reports on the affected primary target and the underlying effective causal mechanisms. So in this study, to better understand the risk of BTX (benzene, toluene and o-xylene) which are the major VOCs and to identify novel biomarkers on immune response to these VOCs exposure in human T lymphocytes, we performed the toxicogenomic study by analyzing of gene expression profiles using 35 k human oligo-microarray. BTX generated specific gene expression patterns in Jurkat cell line. By clustering analysis, we identified some genes as potential markers on immuno-modulating effects of BTX. Four genes of these, HLA-DOA, ITGB2, HMGA2 and 5TAT4 were the most significantly affected by BTX exposure. Thus, this study suggests that these differentially expressed immune genes may play an important role in the pathogenesis on BTX exposure and have significant potential as novel biomarkers of exposure, susceptibility and response to BTC.

The Effect of Rancid perilla oil diet on the Immune Response in Mice (마우스에 있어서 부패들기름 식이가 면역반응에 미치는 영향)

  • 안영근;김정훈;박영길
    • Environmental Analysis Health and Toxicology
    • /
    • v.3 no.1_2
    • /
    • pp.9-19
    • /
    • 1988
  • The effect of rancid perilla oil on the immune response in mice was studied. ICR male mice were divided into 5 groups and were fed on the experimental diets for 4 weeks. Mice were sensitized and challenged with sheep red blood cell. Immune responses were evaluated by antibody production, Arthus reaction, delayed type hypersensitivity (DTH), Rosette forming cell and macrophage activity. Biochemical items were measured by serum protein and serum albumin. The weight of spleen, thymus and liver were measured. The rancid perilla oil diets decreased humoral and cellular immune responses, the number of peripheral circulating white blood cells and total protein and serum albumin. These results showed that the high rancid perilla oil diet decreased more humoral and cellular immune response, the number of peripheral circulating white blood cells, and total protein and serum albumin than the low rancid perilla oil diet did.

  • PDF