• Title, Summary, Keyword: cold resistance

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Fitness is Recovered with the Decline of Dimethachlon Resistance in Laboratory-induced Mutants of Sclerotinia sclerotiorum after Long-term Cold Storage

  • Li, Jin-Li;Wu, Feng-Ci;Zhu, Fu-Xing
    • The Plant Pathology Journal
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    • v.31 no.3
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    • pp.305-309
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    • 2015
  • After four years of cold storage, dimethachlon resistance of two laboratory-induced resistant Sclerotinia sclerotiorum isolates SCG7 and LA50 declined by 99.5% and 98.9%, respectively, and cross resistance to iprodione and procymidone also declined dramatically. Along with the decline of fungicide resistance, osmotic sensitivity to sodium chloride and glucose decreased tremendously; mycelial growth rate, sclerotia number and weight per potato dextrose agar (PDA) plate increased on average by 118.6%, 85. 5% and 64.5%, respectively; and virulence to detached leaves of oilseed rape increased by 72.7% on average. Significant negative correlations were detected between dimethachlon resistance levels and mycelial growth rate on PDA (r = -0.980, P = 0.021), and between resistance levels and lesion diameters on detached leaves of oilseed rape plants (r = -0.997, P = 0.002). These results have profound implications for assessing the potential risk for resistance development to dicarboximide fungicides in S. sclerotiorum.

Differentially Expressed Genes Related to Cold-resistance in Barley (Hordeum vulgare L. cv. Nagaoka)

  • Chun, Jong Un;Park, Jeong-Seon;Bae, Chang-Hyu;Shin, Jeong-Sheop
    • Korean Journal of Breeding Science
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    • v.41 no.2
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    • pp.73-83
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    • 2009
  • To investigate genes related to vernalization and cold- resistance in barley (Hordeum vulgare L. cv. Nagaoka), differentially expressed genes were identified from cold-resistant barley leaves with suppression subtractive hybridization (SSH) and Northern blot analyses. The nucleotide and the deduced amino acid sequences of the putative gene products were compared. The bvrn-7 showed high homology(84%) with gene related to vernalization, and the bvrn-3, bvrn-12, bvrn-28, bvrn-29 and bvrn-36 related to cold-resistant genes had high identity of 88~98% with low temperature-induced genes. The results indicate that the 6 genes were closely related to vernalization and cold-resistance during low temperature treatment.

Finger temperature Response According to Daily Life of Female College Student (여대생의 생활 습관에 따른 국소한랭혈관 반응)

  • Kim, Yang-Weon;Song, Eun-Young
    • Korean Journal of Human Ecology
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    • v.20 no.1
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    • pp.195-203
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    • 2011
  • The purpose of this study was to define the effects of the finger temperature response according to the daily life of college student. For this study, 31 healthy female college students were taken as a subject group. To define the effects of the finger temperature response, housing style, subjective thermal sensations during daily life in the house and domestic working time were surveyed. The finger temperature response items were measured. The results were as follows. Strong, normal and weak group members were divided according to their cold resistance index(RI) 3, 8, 20 people, repectively. Subjective thermal sensations during daily life in the house affects the cold resistance index(p<.01). The cold resistance index(RI) got higher as domestic working time was increased(p<.05, F-value=3.927). The percentage wearing protective gloves during domestic work in the weak group was higher than the normal or strong groups. Subjective sensations during daily life and domestic working time effected the local cold tolerance, living in a comfortable environment continuously can weaken one's cold tolerance.

A Study on Composites of Ethylene-Vinyl Acetate Copolymer and Ethylene-Propylene-Diene Rubber with Aluminum Hydroxide as a Fire Retardant

  • Lee, Yu Jun;Lee, Su Bin;Jung, Jae Young;Lee, Dam Hee;Cho, Ur Ryong
    • Elastomers and Composites
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    • v.51 no.2
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    • pp.93-98
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    • 2016
  • The composites of EVA/EPDM including aluminum trioxide (ATH) as a fire retardant were manufactured for the purpose of improving low temperature property and flame resistance in the rubbery materials. The ratio of EVA to EPDM didn't affect the flame resistance of the rubber composites. The addition of ATH resulted in increase of the flame resistance. In the evaluation of the cold resistance, the increasing EPDM content showed enhancement of cold resistance in the composites due to increasing low Tg EPDM. It was found out that tensile strengths of the composites showed a maximum value at 100 phr of ATH by reinforcing effect, but a minimum value at 200 phr of ATH owing to slippage between the flame retardant by the external stress. In the measurement of solvent resistance in tetrahydrofuran, the increasing ATH content yielded enhancement of solvent resistance by reducing swelling of the composite, and increasing EPDM content also resulted from increase of the solvent resistance by reduction of polarizability as well as increase of crosslink in the composites.

Studies on the Cold Resistant in Rape, Brassica napus L. (유채 내한성에 관한 연구)

  • Kim, I.H.;Kwon, B.S.;Lee, J.I.;Yu, I.S.;Kim, J.K.;Lee, Y.B.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.22 no.1
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    • pp.26-34
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    • 1977
  • 27 lines were selected for the cold resistant lines from the promising lines. The cold resistant lines were chiefly selected from Oro$\times$Norin 16, Oro$\times$Norin 20 and (Yudal$\times$Kongo)$\times$Yudal combination. The varieties of lately maturing showed cold resistantce. Transplanting treatment increased cold resistantce, and early sowing and drill planting also elevated cold resistance in the direct sowing culture. Plants to be cultured on soils with optimum moisture coutent showed higher cold resistance.

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Evaluation of Crack Resistance of Cold Joint as Usage of Sealing Tape (실링 테이프 적용에 따른 시공조인트 균열 저항성 평가)

  • Lee, JaeJun;Lee, Seonhaeng;Kim, Du-Byung;Lee, Jinwook
    • International Journal of Highway Engineering
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    • v.20 no.3
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    • pp.1-9
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    • 2018
  • PURPOSES : In order to evaluate a crack resistance at cold joint, sealing tape was adopted to apply at cold joint instead of typical tack coat material(RSC-4). The sealing tape was made by hot sealing material. The crack resistance as function of environmental and traffic loading was measured with visual observation. METHODS : In this study, the crack resistance was evaluated as function of environmental and traffic loading. The freeze-thaw method was adopted for environmental loading of asphalt pavement. condition. The damage of cold joint under freeze-thaw action is initiated by ice expansion load and accelerated by the interfacial damage between new and old asphalt pavement. The traffic loading was applied with wheel tracking machine on the cold joint area of the asphalt pavement for 3 hours at $25^{\circ}C$. The evaluation of crack resistance was measured with visual observation. The freeze-thaw results shows that the sealing tape was significantly increased the crack resistance based on. RESULTS : To estimate the crack resistance at cold joint area due to the environmental loading, the Freeze-thaw test was conducted by exposing the product to freezing temperature(approximately $-18^{\circ}C$) for 24 hours, and then allowing it to thaw at $60^{\circ}C$ for 24 hours. The tack coat material(RSC-4) was debonded after 21 cycles of the Freeze-thaw test. The first crack was observed after 14 freeze-thaw cycle with RSC-4 material. But, the sealing tape was not debonded after 24 cycle test. Also, the sealing tape shows the better performance of the crack resistance under the traffic loading with wheel track test. The crack was generated the under traffic loading with RSC-4(tack coating), however, the crack was not shown with sealing tape. It indicates that the sealing tape has a strong resistance of tensile stress due to traffic loading. CONCLUSIONS :Based on limited laboratory test result, a performance of crack resistance using the sealing tape is better than that of general tack coat material(RSC-4). It means that the sealing tape is possible to extend a pavement service life because the crack, one of the main pavement distresses, will be delayed.

Genetic Analysis of Apoplastic Proteins in Barley Crosses

  • Chun, Jong-Un;Choi, Kap-Seong;Griffith, Marilyn
    • Plant Resources
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    • v.7 no.2
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    • pp.147-154
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    • 2004
  • Antifreeze proteins (AFPs) accumulate in the leaves of barley during cold acclimation, where they may inhibit ice recrystallization and produce freezing resistance of the plant. Four parental diallel crosses of the barley varieties were used to determine the heritability of AFPs and the relationship between the accumulation level of AFPs and freezing resistance. The concentration of apoplastic proteins in the cold-acclimated leaves was increased in the mean by four-fold over as compared with that of nonacclimated. The diallel cross analyses revealed that the gene of Sacheon 6 was dominant and those of Reno and Dongbori 1 were recessive. The AFPs had high narrow-sense heritabilities. The general combining ability effects of Reno and Dongbori 1 were much higher than the other parents. The bands of 32-kD for GLP, 35-& 28-kD for CLP and 25-, 22- & 16-kD for TLP were observed in the apoplastic extracts from cold-acclimated plants, but there were no clear differences between the parents and Fl hybrids. The concentrations of AFPs were significantly correlated with the degree of freezing resistance, indicating that the concentration of AFPs in the plant is the very important factor for freezing resistance.

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Molecular Cloning and Chaperone Activity of DnaK from Cold-adapted Bacteria, KOPRI22215

  • Sung, Min-Sun;Im, Ha-Na;Lee, Kyung-Hee
    • Bulletin of the Korean Chemical Society
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    • v.32 no.6
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    • pp.1925-1930
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    • 2011
  • Psychrophilic bacteria have acquired cold-resistance in order to protect themselves against freezing temperatures, which would otherwise be lethal. DnaK/DnaJ/GrpE systems are molecular chaperones which facilitate proper folding of newly synthesized proteins. Efficient folding processes are of great importance especially in a cold environment, such as the Arctic. In order to understand the protection mechanisms of psychrophilic bacteria against cold temperatures, we have explored a genome of KOPRI22215, tentatively identified as Psychromonas arctica, whose genome sequence has not yet been discovered. With an aim of searching for a coding gene of DnaK from KOPRI22215, we have applied a series of polymerase chain reactions (PCR) with homologous primers designed from other Psychromonas species and LA PCR in vitro cloning. 1917 bp complete coding sequence of dnaK from KOPRI22215 was identified including upstream promoter sites. Recombinant plasmids to overexpress PaDnaK along with EcDnaK (DnaK of E. coli) were then constructed in pAED4 vector and the pET-based system to induce PaDnaK expression by IPTG. Characterization assays of expressed PaDnaK were carried out by measuring survival rates upon 4 day incubation at 4 $^{\circ}C$: a refolding assay as molecular chaperone, and ATPase assay for functional activity. Taking account of all the data together, we conclude that PaDnaK was identified, successfully expressed, and found to be more efficient in providing cold-resistance for bacterial cells.

Temperature Separation Characteristics of a Vortex Tube Based on the Back Pressure of the Cold Air Exit (저온 출구의 배압조건에 따른 볼텍스 튜브의 온도분리 특성 연구)

  • Im, Seokyeon
    • Tribology and Lubricants
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    • v.32 no.5
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    • pp.166-171
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    • 2016
  • Electric vehicle ownership is expanding for two reasons: its technology features have enhanced fuel economy, and the number of vehicle emissions regulations is increasing. Battery performance has a large influence on the capability of electric vehicles, and even though battery thermal management has been actively researched, specific technological improvements to battery performance are not being presented. For instance, many industrial applications utilize vortex tubes as components for refrigeration machines because of their numerous intrinsic benefits. If electric vehicles incorporate vortex tubes for battery cooling, performance and efficiency advancements are possible. This study uses a counter-flow vortex tube to investigate its temperature separation characteristics, based on the back pressure of the cold air exit and the difference between the inlet and back pressures. The experiment uses a vortex tube with the following parameters: six nozzle holes, a 20 mm inner vortex diameter (D), a 14D tube length, a 0.7D cold exit orifice diameter, and a nozzle area ratio of 0.142. The measurements prove that the temperature difference between the hot air and cold air decreased because of the flow resistance of the hot air and the backflow phenomenon at the cold air exit. The flow resistance causes the temperature difference to decrease, and the back pressure of the cold air exit influences the flow resistance. The results show that the back pressure significantly influences the efficiency of temperature separation.

Analysis of the Change of Polyamine during the Cold Treatment in Spring Radish Young Cotyledons and Hypocotyls (봄무우 유식물의 자엽과 하배축에서 저온처리동안 Polyamine 농도 변화의 분석)

  • Lee, Sung Chang;Park, Jae Won;Cho, Bong-Heuy
    • Analytical Science and Technology
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    • v.12 no.1
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    • pp.75-79
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    • 1999
  • The concentration of polyamine and the relationship between the concentration of PA and cold resistance were analysed during the cold stress in the cotyledons and hypocotyls of young spring radishes. The concentration of PUT was increased during the cold stress in the cotyledons and the concentration of PUT and SPD was changed significantly all the stress time. From the results, we suggested that PUT and SPD were synthesized against cold stress or separated from the bound PA during the cold stress. But, the concentration of SPM was not significantly changed during the cold stress. Only SPD existed in the control hypocotyls. The concentration of SPD was increased during the cold stress, but was decreased dramatically during continusly cold stress. The reactivity against stress of hypocotyls was generally more sensitive during the cold treatment than that of the cotyledons because of the lower concentration of PA in the hypocotyls. PUT and SPD were responsible for the resistance of cold stress in the cotyledons and PA did not play an important role on the resistance of cold stress in the hypocotyls. In this study we suggest that PUT and SPD were responsible for the increase of the resistance against the cold stress.

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