• Title, Summary, Keyword: culture medium

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Growth and Pigment Formation of Genus Monascus on Medium Compositions (배지에 따른 Genus Monascus의 증식도와 색소함량)

  • 윤은경;김미정;김순동
    • Korean Journal of Food Preservation
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    • v.9 no.4
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    • pp.425-428
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    • 2002
  • Growth and pigment formation in Genus Monascus(M. pilosus and M purpureus) related 15 kinds of culture media(Lin medium, SP medium, YM medium, YE medium, GMIN medium, SMO medium, MY medium, GY medium, Nishikawa medium, sucrose medium, stock culture, Mizutani medium, modified Lin medium, Toya medium and rice medium) were investigated. Mizutani medium and Lin medium among 15 kinds of the culture media showed good growth for M. pilosus, M purpureus, fresh mycelium weight cultivated for 10 days at 30$^{\circ}C$ was each 24.5∼26.9 or 15.9∼17.2 g/100 mL. The culture media which showed higher content of pigment production in two fungi were Lin medium(OD: 1.2 ∼ 1.6 and Mizutani medium(OD: 0.8 ∼ 1.0) that showed higher in M. pilosus.

Development of Loading Machine of Culture Medium for Oyster Mushroom Production - Investigation of Physical Properties and Element Design of System - (느타리버섯 재배용 배지 입상 장치 개발(1) - 배지 물성 조사 및 장치요소 설계 -)

  • Lee, Kyung-Jin;Lim, Hak-Kyu;Kim, Tae-Han
    • Journal of Biosystems Engineering
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    • v.34 no.4
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    • pp.211-219
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    • 2009
  • In the process of oyster mushroom production, loading work of culture medium needs the most intensive labor power and cost. Therefore, the development of culture medium loading machine causes to reduce the manpower and cost. The main objectives of this study are identify cultivating environment, physical properties of culture medium and to make an element design of culture medium loading machine. The results are summarized as follows: 1. The moisture content and density of popularly used culture medium were 70%(w.b), $26\;kg/m^3$, respectively. 2. Pressure of the blower increased as the impeller speed increased, and the opening ratio of pressure controller decreased. 3. Recommendable c1earance(${\delta}$) between an impeller plate and a blower case was 25 mm at an impeller speed of 3183 rpm 4. Discharge device of type B with a hopper and suit was better than type A with a hopper.

Establishment of In-Vitro Culture System for Enhancing Production of Somatic Cell Nuclear Transfer (SCNT) Blastocysts with High Performance in the Colony Formation and Formation of Colonies Derived from SCNT Blastocysts in Pigs

  • Han, Na Rae;Baek, Song;Lee, Yongjin;Lee, Joohyeong;Yun, Jung Im;Lee, Eunsong;Lee, Seung Tae
    • Journal of Animal Reproduction and Biotechnology
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    • v.34 no.2
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    • pp.130-138
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    • 2019
  • Although somatic cell nuclear transfer (SCNT)-derived embryonic stem cells (ESCs) in pigs have great potential, their use is limited because the establishment efficiency of ESCs is extremely low. Accordingly, we tried to develop in-vitro culture system stimulating production of SCNT blastocysts with high performance in the colony formation and formation of colonies derived from SCNT blastocysts for enhancing production efficiency of porcine ESCs. For these, SCNT blastocysts produced in various types of embryo culture medium were cultured in different ESC culture medium and optimal culture medium was determined by comparing colony formation efficiency. As the results, ICM of porcine SCNT blastocysts produced through sequential culture of porcine SCNT embryos in the modified porcine zygote medium (PZM)-5 and the PZM-5F showed the best formation efficiency of colonies in α-MEM-based medium. In conclusion, appropriate combination of the embryo culture medium and ESC culture medium will greatly contribute to successful establishment of ESCs derived from SCNT embryos.

Influence of pretreatment medium, fresh medium addition, and culture plate size on the production of embryos in isolated microspore culture of hot pepper (Capsicum annuum L.) (고추의 소포자 배양 시 전처리 배지, 새 배지의 첨가, 및 배양 용기의 크기가 배의 생산에 미치는 영향)

  • Park, Eun-Joon;Kim, Jin-Ae;Kim, Moon-Za
    • Journal of Plant Biotechnology
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    • v.36 no.2
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    • pp.184-192
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    • 2009
  • The influences of pretreatment medium, the addition of fresh medium, and the size of culture plate on the production of embryos were investigated in isolated microspore culture of hot pepper (Capsicum annuum L.). Among the media used for heat shock pretreatment ($32{\pm}1{^{\circ}C}$), high frequency embryo production was obtained when the sucrosestarvation medium A was used. On the other hand, neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production. The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further. The embryo production was not improved by the addition of fresh medium after 2 or 3 weeks from starting culture. Increase in the size of the culture plate from $3.5{\times}1.0$ cm to $6.0{\times}1.5$ cm improved embryo quality. These results will provide valuable information for developing an efficient microspore culture system of hot pepper for high frequency embryo production.

Properties of Bacterial Cellulose Produced in Agitated Culture Using Three Kinds of Medium (3 종류의 배지를 이용하여 진탕배양에서 생산된 Bacterial Cellulose의 물성)

  • Park, Sang-Min;Yoon, Sang-Jun;Son, Hong-Joo;Lee, Chung-Yeol;Kim, Hong-Sung
    • Textile Science and Engineering
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    • v.47 no.4
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    • pp.266-271
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    • 2010
  • Bacterial cellulose is formed by microbial Gluconacetobacter xylinus, which produces a cellulose pellicle in its culture medium. The bacterium Gluconacetobacter sp. V6 was cultured in three types of media using an agitation method: a standard Hestrin-Schramm medium and a medium modified with either glycerol or molasses as the carbon sources. The structural properties of the cultured bacterial cellulose, such as crystallinity, viscosity and mechanical properties, were examined. Cell growth was excellent in the agitated culture with the molasses medium, and the cellulose yield was excellent in the agitated culture with the glycerol medium. The agitated culture with the molasses medium resulted in increased cell growth and cellulose yield, as well as improved mechanical properties of cellulose compared to the static culture with the molasses medium.

Production of Tropane Alkaloids by Two-stage Culture of Scopolia parviflora Nakai Adventitious Root

  • Kim, Won-Jung;Jung, Hee-Young;Min, Ji-Yun;Chung, Young-Gwan;Lee, Cheol-Ho;Choi, Myung-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.5
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    • pp.372-377
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    • 2004
  • Scopolia parviflora Nakai, a rare and endangered species, is the sole plant producing tropane alkaloids (TA) among the Korean native species. In order to enhance TA productivity the SP72 root line was selected by screening 100 of root line, and the optimal culture media for root growth and TA production were investigated with the SP72 roots. Based on the several media, SH and 2B5 medium were determined as growth medium and White and NN medium as production medium. Among the four combinations of two-stage culture, 2BN (2B5 as growth medium plus NN as production medium) showed more enhanced root growth and TA production as compared with production media of White and NN medium and growth media of SH and 2B5 medium, respectively. However, bubble column bioreactor (BCB) cultures applying two-stage culture did not reveal the effective results despite of the each successful operation of two-stage culture in conical flasks and BCB cultures.

Plant Regeneration from Protoplasts Isolated through Embryogenic Cell suspension Culture in Rice (벼 현탁배양을 통하여 분리된 원형질체로부터 식물체 재분화)

  • 정병균
    • Journal of Plant Biology
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    • v.36 no.3
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    • pp.211-218
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    • 1993
  • Plant regeneration was accomplished from protoplast culture of rice (Oryza sativa L. cv. Taebaeg). Embryogenic callus was induced from mature seed on MS medium containing 5 mM proline, 2.5 mg/L 2,4-D, 30 g/L sucrose in the dark at 28$^{\circ}C$ and used to establish embryogenic cell suspension culture. Suspension cells were subcultured every one week in N6 medium supplemented with 5 mM proline, 200 mg/L casein hydrolysate, 2.5 mg/L 2,4-D and amino acids of AA medium. Suspension cultures were composed of cells that were densely cytoplasmic, potentially embryogenic and were at least maintained for more than 6 months in liquid medium. Protoplasts were isolated from fast-growing suspension culture cells and cultured in a slightly modified KpR medium by mixed nurse culture. Isolated protoplasts began to divide within 5~7 days and thereafter, protoplast-derived calli were sequentially transferred to callus proliferating medium that soft agar MS medium contained 2 mg/L 2,4-D and produced distinct embryogenic cells. Microcolonies were then transferred to solid medium which consisted of MS medium containing 5 mg/L kinetin, 1 mg/L NAA, 1 mg/L ABA, 30 g/L sucrose and 10 g/L sorbitol under fluorescent light. Mulitple shoots of 4~5 per callus emerged and were transferred to hormone-free MS medium for root initiation. Thereafter, The plantlets were transferred to pots of soil to mature in the culture room.

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Studies on the Recycling Technology of the Waste Paper with Wood Degradable Enzyme(I) - Separation of Crude Enzyme from Wood Degradable fungi - (목질분해효소에 의한 고지의 재활용 기술연구 (제1보) -목질분해균으로부터 조효소의 단리)

  • 양재경;이중명;엄태진
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.29 no.1
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    • pp.43-51
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    • 1997
  • The various culture conditions of Trichoderma viride(ATCC 3454) and Phanerochaete chrysosporium(ATCC 26921) with glucose-pepton medium, Mandels medium, YMG medium for wood degradable enzyme were examined. Mycellium of the two species grew profusely on glucose-pepton medium. Maximum fungal growth was observed about 10days. But CMCase, Fpase, laccase activity in the culture medium with glucose-pepton was not detected. When grown in fermenter culture using Mandels medium, Trichoderma viride produced CMCase and Fpase. Its CMCase activity was 0.15 lU/ml and Fpase activity was 0.3 IU/ml within about 4-6days. Phanerochaete chrysosporium grown in a YMG medium gave the best enzyme activity when they were grown under stationary culture with an atmosphere of 100% oxygen. Levels of laccase activity of 3.0 mull were achieved in stationary culture under 100% oxygen. The enzyme condensation by ultrafiltration method caused a 2-fold(cellulase) and 6-fold(laccase) as compared to control activity.

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Composition of Culture Medium and Culture Conditions for In vitro Culture of Rice Panicle

  • Lee, Jin-Ho;Lee, Ho-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.1
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    • pp.32-37
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    • 2000
  • The in vitro culture of rice panicles is a culturing technique only panicle without other organs in culture solution containing organic substance, so that would be useful to study how assimilate supply affects grain development and maturation. To find the optimum stage for in vitro culture, rice panicles grown in greenhouse were sampled periodically after anthesis and cultured in nutrient medium. The panicles older than 1 weeks after anthesis had produced normal grains. Grain-filling was apparently dependent upon sucrose concentration (8-12 %) in medium, but not affected by nitrogen concentration supplied with glutamine. As far as rice panicle was supplied with sucrose and N in nutrient medium, grains continued accumulation of dry matter and maturation regardless to light condition. Considerably, grain-filling was improved when panicles were positioned horizontally inside flask, so that each grain was partially submerged to nutrient medium.

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Solid Culture Medium Selection Criteria for Hydroponics Farm Households (양액재배 농가의 고형배지 선택 기준에 관한 연구)

  • Kim, Dong-Seok;Kim, Dae-Young;Hwang, Jae-Hyun;Yun, Hoa-Young
    • Korean Journal of Organic Agriculture
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    • v.22 no.4
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    • pp.841-854
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    • 2014
  • This study aimed to analyze the selection criteria and priority settings for solid culture medium used in hydroponic crop production in farm households. Expert brain storming was carried out to extract solid culture medium selection criteria for hydroponic farming. As a result, 3 criteria of economy (cost), productivity, and environment, and 9 factors were extracted. A questionnaire survey of hydroponic farm households was conducted in Gyeonggi, Gangwon, and Chooncheong provinces. AHP analysis of the hydroponic solid culture medium selection criteria identified productivity as the most important criterion, chosen by 58.7% of the respondents, followed by economy (28.4%) and environment (12.9%). The 9 factors were rated by the respondents in the following decreasing order of importance: 1, crop yield (28.3%); 2, pest occurrence (18.5%); 3, maintenance/management costs (12.0%); 4, convenience of maintenance/management (13.4%); 5, initial investment cost (11.6%); 6, material energy consumption (6.5%); 7, waste recyclability (4.0%); 8, waste disposal costs (3.4%); and 9 environmental emissions (1.81%). These results imply that hydroponic farm households consider cultivation-related quality factors more important than economic factors, such as price of culture medium or installation cost.