• Title, Summary, Keyword: ddY mouse

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Mouse Strain-Dependent Osteoclastogenesis in Response to Lipopolysaccharide

  • Choi, Ho-Gil;Kim, Jin-Moon;Kim, Bong-Ju;Yoo, Yun-Jung;Cha, Jeong-Heon
    • Journal of Microbiology
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    • v.45 no.6
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    • pp.566-571
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    • 2007
  • Bacterial lipopolysaccharide (LPS) is a potent stimulator of bone resorption in periodontitis. Co-culture systems of mouse calvaria-derived osteoblasts and bone marrow-derived preosteoclasts were used as an in vitro osteoclast differentiation. This study revealed that co-cultures using ddY or ICR mouse strain responded differently to LPS while responded equally to $1{\alpha},25(OH)_2D_3$. Thus, the different response to LPS indicates dissimilarity of two mouse stains in their capacity for generating osteoclasts while the two mouse strains share the similarity in response to $1{\alpha},25(OH)_2D_3$. To identify which cells between osteoblasts and preosteoclasts in the co-culture are responsible for the dissimilarity, the reciprocal co-cultures were performed between ddY and ICR mouse strains. The treatment of $1,25(OH)_2D_3$ to ddY/ICR (osteoblasts from ddY/preosteoclasts from ICR) and ICR/ddY reciprocal co-cultures also showed the similarity. In case of LPS treatment, the results of ddY/ICR were similar to ddY/ddY and the results of the other reciprocal co-culture, ICR/ddY combination, were consistent with those of ICR/ICR. It suggests that the dissimilarity between the two mouse strains may resident in osteoblasts but not in preosteoclasts. Therefore, the osteoblast is responsible for mouse strain-dependent osteoclastogenesis in response to LPS. Although mouse models will continue to provide insights into molecular mechanisms of osteoclastogenesis, caution should be exercised when using different mouse strains, especially ddY and ICR strains as models for osteoclast differentiation.

A comparative study on the osteoporotic animal models in ddY mouse: ovariectomized and neurectomized models (ddY 마우스를 이용한 골다공증 모델에 대한 비교연구: 난소적출 및 신경절단 모델)

  • Lee, Hyeung-sik;Hong, Pyo-one;Ku, Se-kwang;Lee, Jae-hyun;Ham, Tae-Su
    • Korean Journal of Veterinary Research
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    • v.44 no.4
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    • pp.487-495
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    • 2004
  • In order to compare the induced time of osteoporosis between ovariectomized and neurectomized models in ddY mice. Experimental groups were divided into Sham, ovariectomized (OVX group) and neurectomized (NX group) group. The changes of body weight, tibia weight and histomorphometry of epiphyseal regions of tibia that were generally used as criteria index in osteoporosis, were evaluated at 2 and 4 weeks after operations with other generally used index-changes of serum osteocalcin. Also, calcium and phosphorus levels in the ash tibia were demonstrated with their ratio (Ca/P ratio). From the result of this study, evidences which reflect osteoporotic states of animals such as decrease of absolute and relative tibia weight, histomorphometrical index of epiphyseal region of tibia including trabecular bone volume %, and calcium and phosphorous contents in tibia, were generally detected from 4 weeks after ovariectomy and 2 weeks after neurectomy with increase of serum osteocalcin levels. In conclusion, it is considered that more rapid and favorable osteoporosis was induced in neurectomized model compared to that of ovariectomized model.

Effects of Taurine and Orotic Acid on Lipid Concentration of Hepatic and Serum in Mouse (마우스의 간장과 혈청 지질농도에 미치는 타우린 및 오로트산의 영향)

  • 차재영;조영수
    • Journal of Life Science
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    • v.9 no.6
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    • pp.659-664
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    • 1999
  • Male mice (ddY strain) were fed with the chow diet containing 10% sucrose supplemented with orotic acid at the 1% level or/and taurine at the 5% levels for 14 days. The concentrations of triacylglycerol and cholesterol in the liver were significantly lower in the orotic acid group than the control group. When taurine and orotic acid were administered simultaneously, the concentrations of triacylglycerol and cholesterol in the liver were higher and lower, respectively, compared to the orotic acid group. The concentration of triacylglycerol in the serum was higher in the taurine group than that of the control or the orotic acid groups, and the simultaneous supplementation of orotic acid and taurine further enhanced. There were no significantly difference in body weight gain, diary food intake, and the concentrations of serum cholesterol and hepatic phospholipid. These results suggest that dietary taurine stimulated the increasion of hepatic triacylglycerol by orotic acid in mouse.

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In vitro and In vivo Evaluation of Genotoxicity of Stevioside and Steviol, Natural Sweetner (천연감미료 스테비오사이드와 스테비올의 생체내, 시험관내 유전독성평가)

  • 오혜영;한의식;최돈웅;김종원;손수정;엄미옥;강일현;강혁준;하광원
    • YAKHAK HOEJI
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    • v.43 no.5
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    • pp.614-622
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    • 1999
  • The standard operation procedure of mouse lymphoma L5178Y $tk^{+/-}-3.7.2C$ gene mutation assay (MOLY) has been regarded as a sensitive in vitro mammalian cell gene mutation assay that is capable of detecting clastogens as well as mutagens. Using MOLY, one of natural sweetner, stevioside (5mg/ml) and its aglycon, steviol ($340{\;}\mu\textrm{g}/ml$) were evaluated the mutagenicity. Stevioside and steviol did not induce mutagenicity in MOLY. On the other hand, stevioside (250mg/kg, B.W.) and steviol (200mg/kg, B.W.) were also evaluated their ability to induce micronuclei in regenerating hepatocytes and bone marrow cells of ddY mice. From these results, stevioside and steviol did not induce any mutagenic effect both MOLY and in vivo micronucleus test.

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Genetic Toxicity Study of YH1715 Series, Antifungal Agents (YH1715계열 항진균제의 유전독성평가)

  • 하광원;오혜영;박장환;허옥순;손수정;한의식;이종영;김소희;강희일
    • Environmental Mutagens and Carcinogens
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    • v.18 no.2
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    • pp.93-97
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    • 1998
  • The results of chromosome aberration test in mammalian cells in culture (Chinese hamster lung fibroblast cells) showed no induction of structural and numerical aberrations by antifungal agents of YH1715 series regardless of metabolic activation. While positive control group (mitomycin C and benzo(a)pyrene) showed structural chromosome aberrations of 37% and 23%, respectively. The in vivo induction of micronuclei was measured in polychromatic erythrocytes in bone marrow of male ddY mouse given YH1715R and YH1729R at 1, 0.5, 0.25 g/kg by p.o. once. After 24 hours, animals were sacrificed and evaluated 40 the incidence of micronucleated polychromatic erythrocytes in whole erythrocytes. Although a positive response for induction of micronuclei in animals treated with mitomycin C demonstrated the sensitivity of the test system for detection of a chemical clastogen, YH1715R did not induce micronuclei in bone marrow of ddY male mice but induced cytotoxicity to bone marrow cells at the highest concentration (1 g/kg, p〈0.05), and YH1729R induced micronuclei in bone marrow of ddY male mice dose dependently (p<0.05) but did not induce cytotoxicity to bone marrow cells.

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Genetic Toxicity Studies of YH1226, a Cephalosporin Antibiotic (세파계 항생제, YH1226의 유전독성 평가)

  • 허광원;오혜영;박장환;허옥순;순수정;한의식;김명희;강희일
    • Environmental Mutagens and Carcinogens
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    • v.18 no.2
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    • pp.89-92
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    • 1998
  • The results of chromosome aberration test in mammalian cells in culture (Chinese hamster lung fibroblast cells) showed no induction of structural and numerical aberrations by YH1226, a cephalosporin antibiotic regardless of metabolic activation, while positive control group (mitomycin C and benzo(a)pyrene) showed structural chromosome aberrations of 25% and 10%, respectively. The in vivo induction of micronuclei was measured in polychromatic erythrocytes in bone marrow of male ddY mouse given YH1226 at 500, 250, 125 mg/kg by i.p. once. After 24 hours, animals were sacrificed and evaluated for the incidence of micronucleated polychromatic erythrocytes in whole erythrocytes. Although a positive response for induction of micronuclei in animals treated with mitomycin C demonstrated the sensitivity of the test system for detection of a chemical clastogen, YH1226 did not induce microunclei in bone marrow of ddY male mice.

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CHEMOPREVENTIVE EFFECTS OF ETHYL 3-(4'-GERANYLOXY-3-METHOXYPHENYL)-2-PROPENOATE AND FERULIC ACID ON MOUSE COLON CARCINOGENESIS

  • Han, Beom-Seok;Shin, Dong-Whan;Yum, Young-Na;Cho, Jeong-Sik;Yang, Ki-Wha;Nobuo Takasuka;Tetsuyuki Takahashi;Hiroyuki Tsuda
    • Proceedings of the Korean Society of Toxicology Conference
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    • pp.74-74
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    • 2002
  • Ethyl 3-(4'-geranyloxy-3-methoxyphenyl)-2-propenoate (EGMP) and ferulic acid (FA) have been shown to inhibit development of aberrant crypt foci (ACF) in the azoxymethane (AOM)-treated rat colon. In the present study, inhibitory effects of EGMP and FA on the post-initiation stage of AOM-induced colon carcinogenesis were studied in male ddY mice.(omitted)

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TOXICITY STUDY ON CHINESE HERBAL DRUGS USING THE MICRONUCLEUS ASSAY IN MURINE BONE MARROW ERYTHROCYTES

  • Ian C. Guest;Yoo, Sang-Ou;Paik, Nam-Woo;Lee, Young-Wook;Oh, Ki-Bong;Yang, Heyong-Cheol;Suh, Nan-Joo;Chang, Il-Moo
    • Toxicological Research
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    • v.5 no.2
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    • pp.71-77
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    • 1989
  • A mouse whole animal bioassay was employed to screen for potential mutagenicity of ethanol/water extracts of 16 Chinese herbal drugs that are commonly prescribed in Korea. Specific cytogenetic toxicity was measrured by recording evidence of clastogenesis toxicity was measured by recording evidence of clastogenesis via the mouse bone marrow micronucleus test. Male ICR mice administered ethanol extract of Pinelliae tuber (Pinellia eternata Breitenbach, ARACEAE, 양복) and ddY female mice administered extract of Angelica Koreanae radix(Angelica Koreana Maximowicz, UMBELLIFERAE, ) (both by oral administration, at a dose of 600 mg/kg), in a short-term dosing schedule, demonstrated significant increase in micronucleated polychromatophilic erythrocytes, indicating the increase of clastogenicity.

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Effect of Water Extract from Stem Bark of Rhus verniciflua Stokes on the Concentrations of Lipid and Lipid Peroxidation in Mice (옻나무 수피 추출물이 마우스의 지질농도 및 지질과산화에 미치는 영향)

  • 차재영;조영수
    • Journal of Life Science
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    • v.10 no.5
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    • pp.467-474
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    • 2000
  • Male mice (ddY strain) were fed a laboratory chow diet containing the water extract from stem bark of Rhus verniciflua Stokes (RVS) for 14 days. There were no significant difference in body weight gain, feed intake, the hepatic lipid profile and serum total cholesterol and phodpholipid concentrations. The concentration of triglyceride in serum was significantly lower in the RVS group than that in the control group. The concentration of high-density-lipoprotein cholestrol in serum was significantly higher in the RVS group than that in the control group. The methanol extract from RVS stem bark effectively inhibited the formation of thiobarbituric acid-reactive substances as a marker of lipid peroxidation of liver microsomes in a concentration-dependent manner. This study showed that the water extract from stem bark of RVS decreased the serum triglyceride concentration and methanol extract has an antioxidative activity.

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Effect of Dietary Orotic Acid on Triglyceride Metabolism in Rats and Mice (Orotic acid 유발 지방간 rat 와 mouse의 중성지질 대사)

  • 조영수;차재영
    • Journal of Life Science
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    • v.6 no.3
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    • pp.159-164
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    • 1996
  • Effects of 1% dietary orotic acid on triglyceride metabolism were examined in SD-rats and Kud: ddY mice. When rats were fed semisynthetic diet containing 1% orotic acid and n-6 polyunsaturated fatty acid (linoleic acid), the hepared diet. In contrast to rats which respond to orotic acid consumption with increases in hepatic triglyceride content, mice did not so respond. The rats-limiting step in triglyceride synthesis is catalyzed by the enzyme phosphatic acid phosphohydrolase (EC3.1.3.4) which is present in the liver cytosol and microsomes of rats fed oroic acid diet. This finding suggests that the activity of this enzyme may play a tole in the fatty liver formation in rats.

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