• Title, Summary, Keyword: decursin

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Environmental Variation of Decursin Content in Angelica gigas (재배지역에 따른 참당귀의 Decursin 함량변이)

  • 성낙술;이순우;김관수;이승택
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.1
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    • pp.60-65
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    • 1993
  • This experiment was conducted to understand the effects of climatic environment on the useful components in Angelicae gigantis radix. The variation of decursin contents in different seven cultivated regions and the relationship between decursin contents and meteorological factors were investigated. The appropriate analytical methods for decursin and decursinol angelate of Angelicae gigantis radix were HPLC method used normal phase column($\mu$-Porasil) and GC method. In different cultivated regions, the variation of decursin contents of Angelicae gigantis radix was relatively great, which were high with 4.86%, 4.75% in Bongwha, Yongcheon respectively and was low with 2.33% in Suwon. There was no significant relationship between decursin contents of Angelicae radix and meteorological factors during growing periods, but was negative correlation in average temperature and precipitation, and positive correlation in diurnal change of temperature, radiation and sunshine hours.

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Isolation and Purification of Decursin and Decursinol Angelate in Angelica gigas Nakai (참당귀(Angelica gigas Nakai) 중 Decursin 및 Decursinol Angelate의 분리 및 정제)

  • Kim, Kang-Min;Jung, Jae-Yeon;Hwang, Sung-Woo;Kim, Myo-Jeong;Kang, Jae-Seon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.5
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    • pp.653-656
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    • 2009
  • This paper is intended as an investigation of the method of extraction and the analysis by high-performance liquid chromatography mass spectroscopy of decursin and decursinol angelate in the dried root of Angelica gigas Nakai. The extracted decursin and decursinol angelate were the purity of >95% using 60% ethanol at $-20^{\circ}C$ for 12 hours by HPLC analysis. Decursin and decursinol angelate were efficiently isolated using recycling HPLC. The purity of isolated decursin and decursinol angelate was identified as 99.97 and 99.40% by HPLC analysis, respectively. The molecular weights of Decursin and decursinol angelate were also identified as m/z=329 ($[M+H]^+$) and m/z=351 ($[M+Na]^+$) by mass spectroscopy.

Effects of Decursin and Decursinol on the Germination and Growth of Plants (Decursin과 Decursinol이 식물의 생장과 발아에 미치는 영향)

  • 이진범
    • Journal of Plant Biology
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    • v.19 no.1
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    • pp.7-13
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    • 1976
  • Biological activities of decursin and decursinol, natural coumarin derivatives, on the germination, growth and adventitious root formation of several plants were observed. In 10 ppm of decursin or decursinol, the growth of Avena coleoptile sections was inhibited, and the activity of IAA-oxidase was gradually enhanced by the increase of its concentrations. Inhibition effect on seed germination was observed from 100 ppm of each chemcials, and the activity of amylase in the germinating seeds was also gradually decreased. However, in the higher concentrations of decursin, the inhibited germination rate of wheat was slightly reduced. Decursin and decursinol also promoted the adventitious root formation in seeds of Phaseolus vulgaris.

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Decursin from Angelica gigas Nakai Promotes Cytotoxicity and Induces Apoptosis in THP-1 cells, a Human Acute Monocytic Leukemia (당귀로부터 정제한 Decursin의 인간 급성 단핵구성 백혈병 세포(THP-1 cells)의 세포 독성 및 Apoptosis에 미치는 영향)

  • Kim, Nam-Seok;Jeong, Seung-Il;Kim, Jong-Seok;Oh, Mi-Jin;Oh, Chan-Ho
    • Korean Journal of Pharmacognosy
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    • v.47 no.3
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    • pp.197-203
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    • 2016
  • Decursin is a major component of the root of Angelica gigas(Umbelliferae), which has been traditionally used in Korea as a tonic and to treat anemia, hemiplegia, and women's diseases. The objective of this study is to identify the anti-cancer mechanism induced by decursin on apoptosis of human leukemia and lymphoma cells. Cytotoxicity of decursin on U937, HL-60, MOLT-4, THP-1 cells showed the significant effects. First of all, $IC_{50}$ of decursin on four cell lines was 27.1, 32.4, 17.4, $15.1{\mu}M$, respectively. So $IC_{50}$ in THP-1 cells was the smallest among 4 cell lines treated with decursin($15.1{\mu}M$). In order to understand the apoptosis-mechanism by decursin, we examined the gene expression of bcl-2(anti-apoptotic), bax(pro-apoptotic) and p53(tumor suppressor)after treating the THP-1 cells with decursin(10, 50 and $100{\mu}M$). It was found bcl-2 gene was decreased dose dependently, the expression level of bax gene of THP-1 cells treated with $100{\mu}M$ of decursin was about 3 times higher than those of control, and p53 gene was increased In the same concentration($100{\mu}M$), p53 gene was increased dose dependent manner. In protein express, bcl-2 and p53 protein showed a tendency to decrease. bax was increased about 4 fold. Therefore decursin is a useful chemotherapeutic agent against leukemia.

Decursin induces apoptosis in glioblastoma cells, but not in glial cells via a mitochondria-related caspase pathway

  • Oh, Seung Tack;Lee, Seongmi;Hua, Cai;Koo, Byung-Soo;Pak, Sok Cheon;Kim, Dong-Il;Jeon, Songhee;Shin, Boo Ahn
    • The Korean Journal of Physiology and Pharmacology
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    • v.23 no.1
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    • pp.29-35
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    • 2019
  • Decursin is a major biological active component of Angelica gigas Nakai and is known to induce apoptosis of metastatic prostatic cancer cells. Recently, other reports have been commissioned to examine the anticancer activities of this plant. In this study, we evaluated the inhibitory activity and related mechanism of action of decursin against glioblastoma cell line. Decursin demonstrated cytotoxic effects on U87 and C6 glioma cells in a dose-dependent manner but not in primary glial cells. Additionally, decursin increased apoptotic bodies and phosphorylated JNK and p38 in U87 cells. Decursin also down-regulated Bcl-2 as well as cell cycle dependent proteins, CDK-4 and cyclin D1. Furthermore, decursin-induced apoptosis was dependent on the caspase activation in U87 cells. Taken together, our data provide the evidence that decursin induces apoptosis in glioblastoma cells, making it a potential candidate as a chemotherapeutic drug against brain tumor.

Anti-Proliferation Effects of Decursin from Angelica gigas Nakai in the MCF-7 Cells Treated with Environmental Hormones (환경호르몬에 의해 유도된 인체 유방암세포의 증식에 대한 당귀로부터 분리한 Decursin 억제효과)

  • Park, Kyung-Wuk;Choi, Sa-Ra;Yang, Hee-Sun;Cho, Hyun-Wook;Kang, Kap-Suk;Seo, Kwon-Il
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.7
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    • pp.825-831
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    • 2007
  • Anti-proliferation effects of decursin from Angelica gigas Nakai were investigated in the MCF-7 cells treated with environmental hormones. The proliferation was decreased in a dose-dependent manner at the concentration over 20 ${\mu}g/mL$ in the MCF-7 cells treated with decursin of various concentrations. The environmental hormones such as $17{\beta}$-estradiol and bisphenol increased the growth of MCF-7 cells in the charcoal-treated FBS (cFBS) medium and the proliferation was the highest at 0.1 ${\mu}M$ among the tested hormone concentration. Decursin was predicted to inhibit the proliferation in a dose-dependent fashion at tested concentrations (1, 3, 10 or 30 ${\mu}g/mL$) in the MCF-7 cells added environmental hormones; however, the survival rate of the cells was lower than that of control cells that were not treated with decursin at 30 ${\mu}g/mL$ concentration. The chromatin condensation and apoptotic body were examined in the decursin treated cells cultured with the cFBS medium added environmental hormones. These results suggest that decursin decreased the proliferation through apoptosis in the MCF-7 cells added environmental hormones.

Decursin from Angelica gigas Nakai Blocks hKv1.5 Channel

  • Kwak, Yong-Geun;Choi, Bok-Hee;Kim, Dae-Keun;Eun, Jae-Soon
    • Biomolecules & Therapeutics
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    • v.19 no.1
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    • pp.33-37
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    • 2011
  • Decursin was purified from Angelica gigas Nakai, and its effects on the human Kv1.5 (hKv1.5) currents were recorded in mouse fibroblasts ($Ltk^-$ cells) by whole-cell patch-clamp technique. Decursin inhibited hKv1.5 current in a concentration-dependent manner, with an $IC_{50}$ value of $2.7\;{\mu}M$ at +60 mV. Decursin accelerated the inactivation kinetics of the hKv1.5 channel, and slowed the deactivation kinetics of the hKv1.5 current, resulting in a tail crossover phenomenon. Also, decursin inhibited the hKv1.5 current in a use-dependent manner. These results strongly suggest that decursin is a kind of open-channel blocker of the hKv1.5 channel.

Determination of Biosynthetic Pathway of Decursin in Hairy Root Culture of Angelica gigas

  • Ji, Xiuhong;Huh, Bum;Kim, Soo-Un
    • Applied Biological Chemistry
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    • v.51 no.4
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    • pp.258-262
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    • 2008
  • To establish the biosynthetic pathway of decursin in Angelica gigas Nakai, feeding experiment with stable isotope-labeled precursors were conducted. Umbelliferone and decursin were labeled with deuterium at C-3. The umbelliferone, the decursin, and other commercially available putative precursors, L-phenylalanine-ring-$d_5$ and trans-cinnamic acid-$d_7$, were fed to the hairy root culture of A. gigas. Each deuterated compound was incorporated into decursinol, decursinol angelate, and decursin as determined by mass spectrometric analysis. These findings confirmed the coumarin biosynthesis pathway sequence is composed of phenylalanine, cinnamic acid, umbelliferone, decursinol, and decursin.

Nodakenin and Decursin Contents of Fermented Angelicae Gigantis Radix by 4 Species Strain (4종의 발효균주가 당귀의 nodakenin과 decursin의 함량에 미치는 변화)

  • Park, Jin-Han;Jung, Ji-Wook;Kweon, Kee-Tae;Seo, Min-Jun;Seo, Eun-Kyoung;Park, Yong-Ki;Lee, Je-Hyun
    • The Korea Journal of Herbology
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    • v.25 no.4
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    • pp.7-10
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    • 2010
  • Objectives : The purpose of this study was to investigate the nodakenin and decursin contents in each fermented Angelicae gigantis Radix by 4 species of ferment strains. Methods : The strains of fermented Angelicae gigantis Radix were Lactobacillus plantarum(SE1), L. acidophilus(AC), Bacillus subtilis(B2) and B. licheniformis(BL2). The fermentative changes of nodakenin and decursin were analyzed using HPLC. Results : All of 4 species strains reduced nodakenin and decursin concentration in Angelicae gigantis Radix by fermentation. But fermentability were difference between strains. The nodakenin concentration of AC was lower than B2. The decursin concentrations of SE1 and AC were lower than B2 and BL2. Conclusions : 4 species of fermental strain were difference decomposable rate with nodakenin and decursin in fermented Angelicae gigantis Radix.

Effect of N, P and K Application on the Contents of the Decursin and Total Sugar in Angelicae gigantis Radix (N,P,K 시용(施用)이 당귀근(當歸根)(Angelicae gigantis Radix)의 Decursin 및 당함량(糖含量)에 미치는 영향(影響))

  • Chang, Sang-Moon;Choi, Jyung
    • Applied Biological Chemistry
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    • v.30 no.1
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    • pp.9-16
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    • 1987
  • This experiment was conducted to increase the available constituents contents of Angelica gigas Nakai and to decide the application amounts of nitrogen, phosphorus and potassium fertilizer. The effects of the amounts of their application and absorption on the contents of decursin and sugar in Angelicae gigantis Redix were studied. The decursin contents of root was decreased with increasing the application amount of nitrogen more than 10kg/10a, and increased along with the increasing application level until 20kg/10a and 15kg/10a of phosphorus and potassium, respectively. The decursin yield per 10a was increased with increasing application level until 10kg/10a, 10kg/10a and 10kg/10a of nitrogen, phosphorus and potassium, respectively. The decursin yield per l0a was decreased with increasing application level more than 20kg/10a of nitrogen. The total sugar yield per 10a was increased with increasing the application level until 15kg/10a, 10kg/10a of nitrogen and potassium respectively. But the effect of phosphorus application on the total sugar yield was not observed. The significant negative correlation was obtained between the decursin contents and the nitrogen contents of root$(r=-0.437^{\ast})$, and the significant positive correlation between the decursin contents and the contents of phosphorus$(r=0.587^{{\ast}{\ast}})$ or potassium$(r=0.467^{\ast})$ in root. But the significant correlations between the decursin contents and the their contents of shoots were not observed. It is recommended for decursin production to apply 10kg/10a, 15kg/10a and 10kg/10a of nitrogen, phosphorus and potassium, respectively.

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