• Title, Summary, Keyword: decursin

Search Result 136, Processing Time 0.035 seconds

Increased Growth by LED and Accumulation of Functional Materials by Florescence Lamps in a Hydroponics Culture System for Angelica gigas (당귀의 수경재배에서 LED 광원에 의한 생장 증가와 형광등에 의한 기능성물질 축적)

  • Lee, Gong-In;Kim, Hong-Ju;Kim, Sung-Jin;Lee, Jong-Won;Park, Jong-Seok
    • Protected Horticulture and Plant Factory
    • /
    • v.25 no.1
    • /
    • pp.42-48
    • /
    • 2016
  • Angelica gigas, belonging family Apiaceae, is a perennial and famous medical plant growing in Korea, Japan, and China. The aims of this study was to analyze the growth and accumulated Decursin and its precursor Decursinol angelate of A.gigas grown under fluorescent lamp and LED. A. gigas 'Manchu' were sowed and managed for seedlings stage in a glass house for 4 weeks. One hundred twenty seedlings with 3 true leafs were transplanted at an ebb & flow system with fluorescent lamp and LED [red: peak wavelength 660nm, blue: peak wavelength 455 nm, white = 3:2:4 ratio] irradiated at $180{\pm}7{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ at the top of plant canopy for 5 weeks. The number of leaves increased by 13.5% in the LED treatment, though it is not statistically significant. Leaf length/width ratio of A. gigas grown under the fluorescent lamps was 24% bigger than the LED treatment and also the stem was 13% larger. Maximum root length was similar to both groups. Fresh weight and dry weight of shoots grown under the LED increased by 50% and 42% and the both weights of roots increased by 125% and 45%, respectively. The contents of Decursin and Decursinol angelate grown under the florescent lamps were larger than LED by 188% and 27% in shoot and 78% and 8% in root. The contents of Decursin and Decursinol angelate per plant grown under LED and florescent lamps were 132mg and 122mg. In conclusion, functional materials in A. gigas were increased by florescent light and its growth was promoted by LEDs light.

Evaluation on the Safety of ${\gamma}$-Irradiated Angelica gigas Nakai: Stability of Active Components and Safety in Genotoxicity Test (감마선 조사 당귀(Angelica gigas Nakai)의 유효성분 안정성 및 유전독성학적 안전성 연구)

  • 조성기;유영법
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.29 no.2
    • /
    • pp.300-306
    • /
    • 2000
  • In the present studies, we assessed the stability of active components and toxicological safety of irradiated Angelica gigas Nakai(Danggui). In order to confirm the stability of active components in the ${\gamma}$-irradiated roots of Danggui, the quantitative analysis of decursin and decursinol angelate of ${\gamma}$-irradiated sample was carried out by high performance liquid chromatographic (HPLC) methods using reverse phase columns and normal phase columns. From the root of Danggui, decursin and decursinol angelate were isolated by a silica gel column chromatography(toluene : ether (1 : 1), Hexane : EtOAc(15 : 1)). And then the structures were confirmed in the 1H and 13C-NMR analysis. The HPLC chromatograms of decursin and decursinol angelate in ${\gamma}$-irradiated Danggui were similar with those of non-irradiated sample. In the examination of in vitro genotoxicity of the water extract from ${\gamma}$-irradiated Danggui using Salmonella reversion assay(Ames test) and micronucleus test in Chinese hamster ovary (CHO) cells, mutagenicity was not exhibited in the two assays with or without metabolic activation. These resutls suggest that active components in the ${\gamma}$-irradiated Danggui should be stable and that the safety of ${\gamma}$-irradiated Danggui could be revealed in further test in vivo.

  • PDF

Quality evaluation of Angelica gigas Nakai with different drying methods and different root parts (당귀(當歸)의 건조방법 및 뿌리 부위에 따른 품질 평가)

  • Seong, Gi Un;Beak, Mi Eun;Lee, Young Jong;Won, Jae Hee
    • The Korea Journal of Herbology
    • /
    • v.33 no.1
    • /
    • pp.85-91
    • /
    • 2018
  • Objectives : This study was carried out to evaluate the quality of different drying methods and different roots(primary root and lateral root) of Angelica gigas Nakai. Methods : The experimental method was performed according to the Korea Pharmacopoea Eleventh edition (KP11). Loss on drying, ash, acid insoluble ash, ethanol extract, nodakenin and total decursin contents were tested to evaluate the quality of root tissue of Angelica gigas Nakai. In addition, the treatment of different root parts were prepared in two groups of washing dry process and natural dry process. Results : In comparison of dry processing methods, total contents of nodakenin and total decursin in the primary root and lateral root through washing dry process were ranged from 3.55 to 4.09% and from 5.18 to 6.13%, respectively. And also, those of roots from the natural dry process were from 4.36 to 6.22% and from 6.28 to 8.34%, respectively. In the washing dry process and natural dry process methods, 47.9% and 22.3% higher amount of nodakenin and total decursin were measured in lateral root compared to primary root. In common, lateral roots accumulated higher contents of nodakenin and total decursin compared to primary roots, and samples drying processed with natural dry process compared to washing dry process method contained higher amount of compounds. Conclusions : We sincerely hope that this study will be contributed to the standardization and quality control of Angelica Gigas Root.

Establishment of Extraction Conditions for Effective Components from Angelica gigas Nakai Using Microwave-Assisted Process (마이크로웨이브 공정을 이용한 당귀 유용성분의 추출조건 설정)

  • 이선영;신승렬;김광수;권중호
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.29 no.3
    • /
    • pp.442-447
    • /
    • 2000
  • 당귀 유용성분의 신속한 추출방법을 개발하기 위한 기초연구로써, 기존 추출방법들에 비하여 환경친화적이고 경제적인 장점이 알려진 마이크로웨이브의 추출조건을 검토하였다. 가용성 당귀성분의 MAP추출에서 당귀의 입자 크기는 60 mesh, 시료와 용매의 비는 1:10(g/mL) 이 적당하였고, microwave power는 80 W에서 당귀성분의 추출효율이 높았다. 가용성 고형분과 decursin 함량을 동시에 가장 많이 추출할 수 있는 50% 에탄올을 용매로 하였을 경우 5분간 3회 반복 추출로써 decursin을 포함한 대부분의 가용성 성분을 추출할 수 있었다.

  • PDF

Studies on the Coumarin Constituents of the Root of Angelica gigas Nakai -Isolation of decursinol angelate and assay of decursinol angelate and decursin- (당귀(當歸)의 Coumarin 성분연구(成分硏究) -Decurson의 구조이성체 Decursinol Angelate의 분리(分離) 및 정량(定量)-)

  • Ryu, Kyung-Soo;Hong, Nam-Doo;Kim, Nam-Jae;Kong, Young-Yun
    • Korean Journal of Pharmacognosy
    • /
    • v.21 no.1
    • /
    • pp.64-68
    • /
    • 1990
  • From the root of Angelica gigas Nakai, decursinol angelate was isolated, which is structural isomer of decursin. Raw material contained average 2.44% of decursinol angelate and 3.30% of decursin by the GC analysis.

  • PDF

Changes of Chloroplast-Mediated Electron Transport Activity and Chlorophyll-Protein Complexes in Barley Seedlings by Decursinol (Decursinol 처리에 따른 보리 유식물의 전자전달 활성과 엽록소-단백질 복합체의 변화에 대하여)

  • 이현식
    • Journal of Plant Biology
    • /
    • v.31 no.2
    • /
    • pp.131-141
    • /
    • 1988
  • The effects of decursinol and decursin on chloroplast-mediated electron transport and phosphorylation in barley seedlings were investigated in comparison with coumarin in the dark or light. The changes of CP-complexes were also studied. Decursinol, decursin and coumarin caused marked inhibitory effects on germination of seed and electron transport and phosphorylation activity of seedlings. The following order of inhibitory effectiveness was exhibited; decursinol>coumarin>decursin. Loss of chlorophyll and decrease of electron transport activity were retarded in the dark, but were reversely accelerated in the light by these three chemicals. The changes of CP-complex patterns were also similar to effects on chlorophyll content and the electron transport activity. These opposite effect in the dark and light suggest that these three chemicals act as natural growth retardants rather than cytokinins or growth inhibitors.

  • PDF

Effects of Some Coumarin Derivatives on the Bovine Lens Aldose Reductase Activity

  • Moon, Chang-Kiu;Lee, Se-Chang;Yun, Yeo-Pyo;Ha, Bae-Jin;Yook, Chang-Soo
    • Archives of Pharmacal Research
    • /
    • v.11 no.4
    • /
    • pp.308-311
    • /
    • 1988
  • Nine coumarin dervatives were examined for thier inhibitory effects on bovine lens aldose reductase activity (bovine-LAR). More than 50% inhibition of BLAR activities was observed in the cases of treatments with decursin, decursinol, esculin, isoimperatorin, oxypeucedanine and isobergapten at the concentration of $10^{-4}$M. Especially, BLAR activity was completely inhibited by the treatment of decursin and decursinol at this concentration. At $10^{-5}$ M, only three coumarins-decursin, decursinol and isoimperatorin, were found to have still relatively higher inhibitory effect.

  • PDF

Effect of the Constituents of Angelicae gigantis Radix on Hepatic Drug Metabolizing Enzymes (참당귀근 성분이 간의 약물대사효소에 미치는 효과)

  • Han, Jung-Mee;Lee, Ihn-Ran;Shin, Kuk-Hyun
    • Korean Journal of Pharmacognosy
    • /
    • v.27 no.4
    • /
    • pp.323-327
    • /
    • 1996
  • The ether soluble fraction of the roots of Angelicae gigantis Radix caused a significant prolongation of hexobarbital(HB) induced sleeping time in mice. Through systematic fractionation of the ether fraction monitored by bioassays, two pyranocoumarins, decursinol angelate and decursin were isolated as active principles. Decursin, as a main component, exhibited significant prolongation of HB-induced hypnosis as well as significant inhibition of hepatic microsomal drug metabolizing enzyme(DME) activities at relatively high dose which indicated that it is a weak DME inhibitor.

  • PDF

Antifungal activities of coumarins isolated from Angelica gigas and Angelica dahurica against Plant pathogenic fungi (당귀와 백지로부터 분리한 Coumarin계 물질들의 식물병원균에 대한 항균활성)

  • Ryu, Shi-Yong;Kim, Young-Sup;Kim, Heung-Tae;Kim, Seong-Ki;Choi, Gyung-Ja;Kim, Jeoung-Seob;Lee, Seon-Woo;Heor, Jung-Hee;Cho, Kwang-Yun;Kim, Jin-Cheol
    • The Korean Journal of Pesticide Science
    • /
    • v.5 no.3
    • /
    • pp.26-35
    • /
    • 2001
  • In order to search potent antifungal substances from domestic plants, 40 plants cultivated in Korea were collected. After extracting with methanol (MeOH) and concentrating to dryness, the MeOH extracts were screened for in vivo antifungal activity against six plant diseases at a concentration of $2000{\mu}g/mL$. Fourteen extracts showed disease-controlling activity more than 90% against at least one of the 6 plant diseases tested; eight, seven, and three extracts controlled more than 90% the development of rice blast, tomato late blight, and wheat leaf rust, respectively. However, none of the extracts exhibited in vivo antifungal activity more than 90% against rice sheath blight, tomato gray mold, and barley powdery mildew. From the MeOH extracts of Angelica gigas and A. dahurica showing potent controlling activity against rice blast, 1 and 2 antifungal substances, respectively, were isolated by solvent partitioning and column chromatography. The three compounds were identified to be coumarins, namely, decursin, imperatorin, and isoimperatorin, by mass spectrometry and NMR spectroscopy. They were examined for in vitro and in vivo antifungal activities together with umbelliferone (7-bydroxycournarin) and scopoletin (6-methoxy-7-hydroxycoumarin) containing a free hydroxyl group at position 7 to investigate the structure-activity relationship. In vitro, most of 50% growth inhibitory concentrations ($IC_{50}$) were over $200{\mu}g/mL$, indicating that they have relatively weak antifungal activity. The antifungal activity of decursin and scopoletin, containing cyclic alkoxy groups instead of free hydroxyl group at position 7, was stronger than umbelliferone and scopoletin. Especially, decursin and imperatorin showed potent antifungal activities against Pythium ultimum and Magnaporthe grisea, respectively, with $IC_{50}$ values less than $25{\mu}g/mL$. In vivo, decursin and imperatorin showed potent antifungal activity against rice blast, whereas other coumarins hardly controlled the development of 6 plant diseases tested. These results suggest that the antifungal activity of 7-hydroxycoumarin derivative is substantially increased when the hydroxyl group at position 7 is protected by a stable cyclic alkoxy grouping.

  • PDF