• Title, Summary, Keyword: decursin

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Synthesis and Biological Evaluation of Decursin, Prantschimgin and Their Derivatives

  • Xia, Yan;Min, Kyung-Hoon;Lee, Kyeong
    • Bulletin of the Korean Chemical Society
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    • v.30 no.1
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    • pp.43-48
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    • 2009
  • The synthesis of coumarin-based natural products and their derivatives is described. In vitro MDR reversal activities of the synthesized compounds were evaluated in P-glycoprotein over-expressing human sarcoma cell line MES-SA/DX5. Some of the coumarin derivatives were found to show potent MDR reversal activity. In particular, pyridyl derivative (15e) exhibited more potency than verapamil.

Variation Mode of the Absorbtion Contents of N, P and K and the Contents of Available Constituents of Angelica gigas Nakai at Different Growth Stages (당귀(當歸)(Angelica gigas Nakai)의 생육시기별(生育時期別) N, P, K 흡수(吸收) 및 유효성분함량(有效成分含量)의 변화양상(變化樣相))

  • Chang, Sang-Moon;Choi, Jyung
    • Applied Biological Chemistry
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    • v.29 no.4
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    • pp.392-398
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    • 1986
  • This study was conducted to find out the variation modes of the absorbtion contents of N, P and K and the contents of decursin, decursinol and sugar in Angelica gigas Nakai at different growth stages. The results of field study were as follows. The dried weights of root and shoot was increased by the application of nitrogen and potassium fertilizers during the growing seasons. But, the effect of phosphorus application was observed only the increasing of the dried weight of shoot. During the growing seasons, the contents of nitrogen and phosphorus in root was decreased, but in shoot was increased. The potassium contents of root was increased and that of shoot was decreased. The decursin and total sugar contents of root were continually increased, and the decursinol and reducing sugar contents were increased until the middle growth stage and after that were decreased.

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Decursin and Z-Ligustilide in Angelica tenuissima Root Extract Fermented by Aspergillus oryzae Display Anti-Pigment Activity in Melanoma Cells

  • Park, Yuna;Kim, Dayoung;Yang, Inho;Choi, Bomee;Lee, Jin Woo;Namkoong, Seung;Koo, Hyun Jung;Lee, Sung Ryul;Park, Myung Rye;Lim, Hyosun;Kim, Youn Kyu;Nam, Sang-Jip;Sohn, Eun-Hwa
    • Journal of Microbiology and Biotechnology
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    • v.28 no.7
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    • pp.1061-1067
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    • 2018
  • The anti-melanogenic effects of the extract of Angelica tenuissima (AT) root and the extract of AT root fermented by Aspergillus oryzae (FAT) were investigated. These effects were determined by measuring the inhibitory activity of AT and FAT on melanin production in B16F10 melanocytes and with in vitro tyrosinase activity assays. The AT extract inhibited melanin production at concentrations above $250{\mu}g/ml$, and this inhibitory effect was significantly enhanced by the fermentation process with A. oryzae. HPLC analysis resulted in the isolation of two active compounds from both the AT and FAT extracts. Their chemical structures were identified as decursin and Z-ligustilide through comparison with previously reported NMR data. The decursin and Z-ligustilide contents were increased in the FAT extract and could be responsible for its enhanced inhibitory effects on melanin production and tyrosinase activity compared with that of the AT extract.

Manufacture of Some Korean Medicinal Herb Liquors by Soaking (몇가지 약초침출주의 제조)

  • Min, Young-Kyoo;Jeong, Heon-Sang
    • Korean Journal of Food Science and Technology
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    • v.27 no.2
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    • pp.210-215
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    • 1995
  • Korean medicinal herbs -sasam, gilkyung, jakyak, danggwi, hwangki and chunkung were soaked to the distillate of Korean rice wine for 75 days. The alcohol concentration of distillate, soaking media was adjusted to 45, 35, and 25% respectively with distilled water. Changes in alcohol concentration, pH, optical density, concentration of peoniflorin and decursin were analyzed. Quality of the final product was determined by sensory evaluation. Alcohol concentration was rapidly decreased but pH increased in 15 days and thereafter they showed slow decrease. Decrease of alcohol concentration was affected by the kind of herb and alcohol concentration of soaking media. The strongest effect was observed from danggwi and low alcohol concentration. Concentration of paeoniflorin and decursin, an index component of jakyak and danggwi respectively, showed the similar trend of decrease after increase to maximum concentration. From the sensory evaluation, the best overall quality was obtained from liquors made from 45% alcohol concentration. The quality was decreased in the order of sasam, jakyak, chunkung and hwanggi.

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Quantitative Analysis of Marker Substances in Solid Fermented Angelicae Gigantis Radix by HPLC (HPLC를 이용한 고체발효 당귀의 지표성분 분석)

  • Um, Young-Ran;Lee, Ji-Hye;Ma, Jin-Yeul
    • Korean Journal of Oriental Medicine
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    • v.16 no.1
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    • pp.173-178
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    • 2010
  • The purpose of this study was investigation of quantitative analysis of marker substances in solid fermented Angelicae Gigantis Radix by High performance liquid chromatography(HPLC). HPLC was performed for determination of nodakenin and decursin in solid fermented Angelicae Gigantis Radix extract, the separation method was performed on C18 column ($250\;mm\;{\times}\;4.6\;mm$, $5\;{\mu}m$, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (330 nm). The flow rate was 1.0 ml/min. Retention time of nodakenin and decursin was about 11.47, 46.79 min and linearity of calibration was showed good result(r2=0.9999, 0.9999), respectively. Content of nodakenin was $0.76\;{\pm}\;0.02%$ in control, $0.31\;{\pm}\;0.00%$ in Angelicae Gigantis Radix extract fermented with Paecilomyces japonica(SDT)(p<0.01), $0.51\;{\pm}\;0.02%$ in Angelicae Gigantis Radix extract fermented with Ganoderma lucidum(SYT)(p<0.01), $0.82\;{\pm}\;0.03%$ in Angelicae Gigantis Radix extract fermented with honey(SST)(p<0.05) and $0.88\;{\pm}\;0.01%$ in Angelicae Gigantis Radix extract fermented with Nuruk(SNT)(p<0.01). Content of decursin was $4.50\;{\pm}\;0.08%$ in control, $2.90\;{\pm}\;0.05%$ in Angelicae Gigantis Radix extract fermented with Paecilomyces japonica(SDT)(p<0.01), $2.65\;{\pm}\;0.08%$ in Angelicae Gigantis Radix extract fermented with Ganoderma lucidum(SYT)(p<0.01), $4.46\;{\pm}\;0.11%$ in Angelicae Gigantis Radix extract fermented with honey(SST) and $4.73\;{\pm}\;0.04%$ in Angelicae Gigantis Radix extract fermented with Nuruk(SNT)(p<0.05), respectively.

Microemulsion Fomulation for Enhanced Topical Absorption of Root Extract of Angelica gigas (당귀 추출물의 피부 흡수 증가를 위한 마이크로에멀젼 조성)

  • Jung, Eun-Jae;Choi, Joon-Ho;Park, Chun-Geon;Choi, Ae-Jin;Jeong, Se-Ho;Chung, Suk-Jae;Shim, Chang-Koo;Kim, Dae-Duk
    • YAKHAK HOEJI
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    • v.56 no.3
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    • pp.152-157
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    • 2012
  • Angelica gigas is one of the most widely used herbal medicines in Asia. Root extract of Angelica gigas is known to have anti-oxidant activity and skin whitening effect. The aim of this study was to prepare microemulsion system of root extracts of Angelica gigas for topical delivery. Microemulsion was successfully prepared by using MCT (medium chain triglyceride) as an oil phase, Labrasol as a surfactant, and the mixture of propyleneglycol and phosphatidylcholine (4 : 1) as a cosurfactant. In vitro and in vivo skin permeation and deposition of decursin, as a marker, was determined using hairless mouse. Microemulsion significantly increased the in vitro skin permeation of decursin for up to 12 hours and was significantly higher than the control (water). Moreover, microemulsion formulation showed significantly higher skin deposition of decursin compared to the control in both in vitro and in vivo studies. Thus, microemulsion could be a useful vehicle for topical application of root extracts of Angelica gigas.

Absorption, Distribution, Metabolism, and Excretion of Decursin and Decursinol Angelate from Angelica gigas Nakai

  • Kim, Kang-Min;Kim, Myo-Jeong;Kang, Jae-Seon
    • Journal of Microbiology and Biotechnology
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    • v.19 no.12
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    • pp.1569-1572
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    • 2009
  • The pharmacokinetics of decursin and decursinol angelate (D/DA) were investigated in male SD rats following oral and intravenous administration. D/DA and metabolites obtained from in vitro samples were evaluated by LC/MS. The levels of D/DA and metabolized decursinol in the blood following oral and intravenous administrations declined according to first-order kinetics, with $T_{1/2}$ values of 56.67, 58.01, and 57.22 h, respectively, being observed after administration of a dose of 2 mg/kg body weight. The large intestine was the major site of disposition following oral administration. These data indicate that D/DA is rapidly absorbed from the gastrointestinal tract. In in vitro experiment utilizing liver microsomal protein, the major metabolic reaction of D/DA occurred to change decursinol. The cumulative biliary, urinary, and fecal excretions of D/DA in bile duct-cannulated rats was $36.10{\pm}2.9%$, $25.35{\pm}3.8%$, and $34.20{\pm}3.2%$, respectively, at 72 h after administration. These results indicate that the absorption of D/DA is almost complete, and that its metabolites are primarily excreted into feces through the bile. These results indicate that D/DA is subject to enterohepatic circulation.

Evaluation of the Genotoxicity of Decursin and Decursinol Angelate Produced by Angelica gigas Nakai

  • Kim, Kang-Min;Kim, Tae-Ho;Park, Yun-Jung;Kim, Ik-Hwan;Kang, Jae-Seon
    • Molecular & Cellular Toxicology
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    • v.5 no.1
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    • pp.83-87
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    • 2009
  • In this study, we assessed the stability and toxicological safety of Angelica gigas Nakai (A. gigas Nakai) extract, which is comprised of decursin and decursinol angelate (D/DA). D/DA was tested for mutagenicity using Ames Salmonella tester strains (TA102, TA1535, and TA1537) with or without metabolic activation (S9 mix). No increase in the number of revertants was observed in response to any of the doses tested (1.25, 12.5, 125, and $1,250{\mu}/mLg$). In addition, a chromosome aberration test was conducted in the Chinese hamster lung (CHL) cell line. To accomplish this, cells were treated with D/DA (3.28, 13.12, 52.46, and $209.84{\mu}g/mL$) or with Mitomycin C ($0.1{\mu}/mLg$) as a positive control in the case of no metabolic activation or benzo(a)pyrene ($20{\mu}g/mL$) in the case of metabolic activation. No significant increase in chromosome aberrations was observed in response to treatment with any of these concentrations, regardless of activation of the metabolic system. According to these results, we concluded that D/DA did not induce bacterial reverse mutation or clastogenicity in vitro in the range of concentrations evaluated in these experiments.