• Title, Summary, Keyword: decursin

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Oral Acute and Subacute Toxicity Studies of Decursin and Decursinol Angelate of Angelica gigas Nakai

  • Kim, Kang-Min;Lee, Young-Jeon;Hong, Yong-Geun;Kang, Jae-Seon
    • Molecular & Cellular Toxicology
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    • v.5 no.2
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    • pp.153-159
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    • 2009
  • In this study, we assessed the acute and subacute toxicity of Angelica gigas Nakai (A. gigas Nakai) extracts, which are comprised of decursin and decursinol angelate (D/DA) in rats. For the oral acute toxicity test, Sprague-Dawley (SD) male and female rats were gavaged with two doses of D/DA (200 and 2,000 mg/kg body weight) and then observed for any toxic symptoms for 2 weeks. The LD$_{50}$ value for the rats was greater than 2,000 mg/kg body weight for both male and female rats, which indicates that there were no toxic symptoms induced by doses of up to 2,000 mg/kg body weight. For the subacute toxicity study, rats were treated with D/DA at doses of 2 and 20 mg/kg body weight once a day for 30 days. There were no significant changes in body weight and food intake observed during the subacute toxicity study. In addition, no differences were observed between the control and treated groups when urinalysis was conducted or when hematology and biochemical parameters were evaluated. Finally, histopathological examination of the organs did not reveal any lesions in the control or treated groups. Taken together, these findings indicate that D/DA is safe and non-toxic.

Effect of a (S)-(+)-decursin Derivative, (S)-(+)-3-(3,4-dihydroxy-phenyl)-acrylic Acid 2,2-dimethyl-8-oxo-3,4-dihydro-2H,8H-pyrano[3,2-g]-chromen-3-yl-ester on Apoptosis of Eosinophils and Neutrophils in Normal and Asthmatic Subjects

  • Kim, In-Sik;Yang, Eun-Ju;Lee, Ji-Sook
    • Biomedical Science Letters
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    • v.18 no.3
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    • pp.307-309
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    • 2012
  • (S)-(+)-3(3,4-dihydroxy-phenyl)-acrylic acid 2,2-dimethyl-8-oxo3,4-dihydro-2H,8H-pyrano[3,2-g]Chromen-3-yl-ester (Compound 6, C6) is synthesized from (S)-(+)-decursin and attenuates the pathophysiologic progression of asthma in a ovalbumin-induced asthmatic mouse model. In the present study, we examined the effect of C6 on spontaneous apoptosis of eosinophils and neutrophils of normal and asthmatic subjects. C6 increased the apoptosis of asthmatic eosinophils in a dose-dependent manner, but it inhibited neutrophil apoptosis. C6 has no effect on apoptosis of normal eosinophils and neutrophils. LY294002, an inhibitor of PI3K, rottlerin, an inhibitor of $PKC{\delta}$, Ro-31-8425, an inhibitor of classical PKC inhibitor, PD98059, an inhibitor of MEK, and BAY 11-7085, an inhibitor of NF-${\kappa}B$, blocked the inhibitory effect on apoptosis of asthmatic neutrophils due to C6. These results indicate that C6 may be valuable as a therapeutic agent for the treatment of asthma.

Characteristic of Growth and Active Ingredient in Angelica gigas Nakai according to Forest Environment by Climate Zone (기후대별 산림환경에 따른 참당귀의 생육 및 지표성분 특성)

  • Kim, Nam Su;Jeon, Kwon Seok;Lee, Hyun Seok
    • Korean Journal of Medicinal Crop Science
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    • v.28 no.3
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    • pp.221-228
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    • 2020
  • Background: Angelica gigas Nakai, that belong to the Umbelliferae family, is one of the traditional medicinal plants in Korea. Its roots have been used to treat gynecological diseases. In this study, growth characteristics and index components were compared with the forest microclimate at several forest sites. Methods and Results: A. gigas was planted in three climatic zones according to the temperature (southern temperature zone - Hamyang, central temperature zone - Bonghwa, and northern temperature zone - Jeongseon) and growth characteristics were investigated in comparison with the forest microclimate. Our results indicated that the root diameter and length, and fresh and dry weight were the highest in Jeongseon. The total content of decursin was the highest in Jeongseon (9.52%), followed by those in Hamyang (8.07%) and Bonghwa (7.48%), respectively. Additionally, the yield of decursin (1.39 g) was the highest in Jeongseon. Conclusions: The yield and index components were influenced by the microclimate in the forests, and it was assumed that high altitude and low temperature affected the increase in growth and index components. These results will be useful as basic data to study the correlation among environmental conditions, growth, and index components.

Changes in the Growth Characteristics and the Content of Decursin and Decursinol angelate in Angelica gigas Nakai by Mineral and Hormonal Agent Treatment (미네랄 및 호르몬 제제 처리에 따른 당귀의 생육특성 및 Decursin, Decursinol Angelate 함량 변화)

  • Lee, Sang Hoon;Yun, Hyeong Muk;Koo, Sung Cheol;Lee, Woo Moon;Chang, Jae Ki;Koo, Hyun Jeong;Chang, Kwang Jin;Kim, Yeon Bok
    • Korean Journal of Medicinal Crop Science
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    • v.26 no.3
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    • pp.227-232
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    • 2018
  • Background: Angelica gigas Nakai is a perennial herb belonging to the family Umbelliferae. Its roots are utilized in traditional medicine. The aim of this study was to increase the yield of and the content of two indicator components (decursin and decursinol angelate) in A. gigas Nakai. Methods and Results: The roots of A. gigas Nakai were harvested 4-times from late August to late October in 2017. Two agents (trace element-TE, and plant hormone-HM) were applied 4 times at intervals of 2 weeks. The content of the two indicator components were analyzed by high performance liquid chromatography. The HM treatment showed the greatest increase in underground part yield and root diameter. The content of the two indicator components in the control (non-treatment) group was the highest in the underground part, but was higher in the aerial parts in the agent treatment group. After treatment with the agents, the content of the indicator components tended to decrease in the underground part. However, the total content of the indicator components in the two agent treatment groups exceeded the level of 6% set by the Korean Pharmacopoeia. Conclusions: The highest underground part yield was found in the HM treatment group, while the highest content of decursin and decursinol angelate were found in the control group. This study provides basic information for yield improvement in A. gigas.

Comparison of Growth and Contents of Active Ingredients of Angelica gigas Nakai under Different Cultivation Areas (재배지 환경에 따른 참당귀 생육 및 지표성분 함량 비교)

  • Kim, Nam Su;Jung, Dae Hui;Jung, Chung Ryul;Kim, Hyun-Jun;Jeon, Kwon Seok;Park, Hong Woo
    • Korean Journal of Plant Resources
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    • v.32 no.5
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    • pp.448-456
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    • 2019
  • Angelica gigas Nakai (Korean danggui), a member of the Umbelliferae family, is a Korean traditional medicinal plant whose roots have been used for treating gynecological diseases. In this study, the growth characteristics and decursin contents of Angelica gigas Nakai were compared according to the difference of cultivation area. As a result, the root diameter of A. gigas Nakai was the highest in the Duil-ri, and the number of the root of A. gigas was the highest in Topdong-ri. The fresh weight and dry weight of Angelica gigas Nakai was the highest in the Topdong-ri. The soil properties of cultivation area was Sandy Clay Loam and the content of organic matter was the highest in Topdong-ri. The total content of decursin was 5.31% the highest in Duil-ri. And 4.96% in Namhoeryong-ri, 4.41% in Kosun-ri, 4.25% in topdon-ri, 3.96% in Gugok-ri, and 3.64% in Gongjeon-ri was accumulated.

Recovery from the Two-generation Reproductive Toxicity in Sprague-Dawley Rats by Treatment with Decursin and Decursinol Angelate (Decursin and decursinol angelate가 2세대 출산률 회복에 미치는 영향)

  • Kim, Kang Min;Ok, Seon;Go, Youn Suk;Kang, Jae Seon
    • Journal of Life Science
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    • v.25 no.7
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    • pp.765-772
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    • 2015
  • The aim of this study was to determine the effect of decursin (D) and decursinol angelate (DA) against bisphenol A (BPA) toxicity in a rat two-generation study. Adult rats were divided into the following three groups: (1) control, (2) BPA, and (3) BPA+D/DA. The D and DA treatment of F0 parents increased the terminal body weight and relative adult organ weights (testes, kidneys, spleen, and liver) when compared with the BPA group. A significant decrease in sperm count was found in the BPA+D/DA (7.69%) and BPA (64.70%, p<0.01) groups, when compared with the sperm count in the control group. No offspring were obtained in the F1 generation of the BPA (50 mg/kg/day) group, but the addition of D/DA in the BPA+D/DA group significantly restored fertility (55.78%) and gestation indices (98.87%) in the F1 generation. No significant differences were found in the fertility index between the control (75.02%) and the BPA+D/DA (78.11%) groups in the two-generation study, when compared with the one-generation study. The viability ratio during lactation in the D/DA group was also similar to that of the control group. These data indicate that D/DA (50 mg/kg/day) administered over two generations causes significant positive changes in reproductive or developmental parameters.

Bioactive Materials and Biological Activity in the Extracts of Leaf, Stem Mixture and Root from Angelica gigas Nakai (참당귀 잎, 줄기혼합물과 뿌리 추출물의 생리활성물질 및 그 활성작용)

  • Heo, Jin-Sun;Cha, Jae-Young;Kim, Hyun-Woo;Ahn, Hee-Young;Eom, Kyung-Eun;Heo, Su-Jin;Cho, Young-Su
    • Journal of Life Science
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    • v.20 no.5
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    • pp.750-759
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    • 2010
  • The bioactive materials (phenolic compounds, flavonoids, minerals, decursin and decursinol angelate) and biological activities (DPPH [$\alpha,\alpha$'-diphenyl-$\beta$-picrylhydrazyl] free radical scavenging capability, reducing power, and tyrosinase activity) in the extracts of leaf, stem mixture (AGLS), and root (AGR) from Angelica gigas Nakai were examined by using water, hot water and ethanol solvent. The highest extract yield (21.89%) was found in the water extract of AGR. The highest concentrations of phenolic compounds and flavonoids in the ethanol extracts of AGLS and AGR were 14.99% and 14.79%. Major minerals of AGLS and AGR were K, Mg, Fe, Na and Ca. Decursin and decursinol angelate were the major ingredients of Angelica gigas, detected at 18.71 and 18.89 min of retention time by HPLC analysis, respectively. The highest concentrations of decursin and decursinol angelate in the Angelica gigas ethanol extract were found in root ($41.7\;{\mu}g/g$) and leaf ($34.04\;{\mu}g/g$). The highest free radical scavenging activity was found in the hot water extracts of AGLS and AGR, and its activity was stronger in all extracts of AGLS than AGR. The highest reducing power was found in the ethanol extracts of AGLS and AGR and this was dependent on the sample concentration. The hot water extracts of AGLS and AGR revealed the highest inhibition activity on tyrosinase. Overall, these results may provide the basic data needed to understand the biological activities of bioactive materials derived from Angelica gigas.

Antibacterial Coumarins from Angelica gigas Roots

  • Lee, Sanghyun;Shin, Dong-Sun;Kim, Ju-Sun;Oh, Ki-Bong;Kang, Sam-Sik
    • Archives of Pharmacal Research
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    • v.26 no.6
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    • pp.449-452
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    • 2003
  • Systematic fractionation of Angelica gigas roots led to the isolation of linear furano(pyrano)coumarins such as bergapten (1), decursinol angelate (2), decursin (3), nodakenetin (4) and nodakenin (5). The antibacterial activities of those compounds against pathogenic bacteria were investigated. Among the compounds tested, decursinol angelate (2) and decursin (3) exhibited significant antibacterial activity against Bacillus subtilis with the minimum inhibitory concentrations (MICs) of 50 and $12.5{\;}{\mu}g/mL$, respectively.

Platelet Anti-Aggregatory Effects of Coumarins from the Roots of Angelica genuflexa and A. gigas

  • Lee, Yong-Yook;Lee, Sang-Hyun;Jin, Jing-Ling;Yunchoi, Hye-Sook
    • Archives of Pharmacal Research
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    • v.26 no.9
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    • pp.723-726
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    • 2003
  • Five coumarins, isoimperatorin (1), pabulenol (2), isooxypeucedanin (3), oxypeucedanin hydrate (4) and osthol (5) were isolated from the MeOH extract of Angelica genuflexa in the course of searching for anti-platelet and anti-coagulant components from plants. Pabulenol (2) was isolated from A. genuflexa for the first time. The five compounds isolated from A. genuflexa, together with decursinol angelate (6), decursin (7) and nodakenin (8) from A. gigas were evaluated for their effects on platelet aggregation and blood coagulation. Compounds 2, 5, 6 and 7 were observed to be either equally effective or 2∼4 times more inhibitory than ASA in both arachidonic acid and U46619 ($TXA_2$ mimetic) induced platelet aggregations.

A Study of the Effects of the Root Components of Angelica gigas Nakai on Voluntary Activity in Mice (참당귀 성분(成分)이 생쥐 자발운동(自發運動)에 미치는 영향(影響))

  • Kim, Hack-Seang;Park, Jung-Sup;Park, Hae-Ja;Chi, Hyung-Joon
    • Korean Journal of Pharmacognosy
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    • v.11 no.1
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    • pp.11-14
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    • 1980
  • A Study of the effects of the root components of Angelica gigas Nakai (Umbelliferae) on voluntary activity in mice has confirmed that there are: 1) MeOH soluble extract, MeOH insoluble extract, decursin and decursinol were antagonized against the voluntary activity in mice pretreated with a dose of caffeine natrium benzoate 30mg/kg. 2) MeOH insoluble extract showed higher depressant effect than MeOH soluble extract. 3) The depressant activity of decursinol was significantly more highly effective than that of decursin.

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