• Title, Summary, Keyword: decursinol

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Isolation and Purification of Decursin and Decursinol Angelate in Angelica gigas Nakai (참당귀(Angelica gigas Nakai) 중 Decursin 및 Decursinol Angelate의 분리 및 정제)

  • Kim, Kang-Min;Jung, Jae-Yeon;Hwang, Sung-Woo;Kim, Myo-Jeong;Kang, Jae-Seon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.5
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    • pp.653-656
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    • 2009
  • This paper is intended as an investigation of the method of extraction and the analysis by high-performance liquid chromatography mass spectroscopy of decursin and decursinol angelate in the dried root of Angelica gigas Nakai. The extracted decursin and decursinol angelate were the purity of >95% using 60% ethanol at $-20^{\circ}C$ for 12 hours by HPLC analysis. Decursin and decursinol angelate were efficiently isolated using recycling HPLC. The purity of isolated decursin and decursinol angelate was identified as 99.97 and 99.40% by HPLC analysis, respectively. The molecular weights of Decursin and decursinol angelate were also identified as m/z=329 ($[M+H]^+$) and m/z=351 ($[M+Na]^+$) by mass spectroscopy.

Comparative Studies on Concentration of Decursinol in Plasma after Oral Administration of Angelicae Gigantis Radix Extract and Combined Use of Decursin and Cnidii Rhizoma Extract or Bupleuri Radix Extract in Rats (당귀와 천궁 및 시호의 병용 경구 투여에서 흰쥐 혈중 Decursinol 농도의 비교 검토)

  • Park, Roh-Jeong;Kim, Nam-Jae;Lee, Kyung-Tae;Seo, Seong-Hoon
    • Korean Journal of Pharmacognosy
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    • v.32 no.1
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    • pp.72-78
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    • 2001
  • This study was performed to elucidate the influence on the plasma decursinol concentration after administration of combined decursin and Cnidii Rhizoma or decurisin and Bupleuri Radix extracts in comparison with single decursin administration. The identification of decursinol isolated from Angelica gigas was carried out by GC/MS and the concentration of decursinol in the plasma after oral administration was determined by HPLC. The value of area under the plasma concentration (AUC) and the maximal concentration (Cmax) of decursinol after administration of methanol and ether Angelicae Gigantis Radix extract was higher than those of single decursin-treated group. It was also found that the AUC and the Cmax of decursinol after combined administration with decursin and Cnidii Rhizoma were higher than those of decursin administration, whereas those of combined with decursin and Bupleuri Radix was lower. Moreover, the studies were performed with decursinol only or with combined decursinol and Cnidii Rhizoma/Bupleuri Radix, both of combined treatment increased the plasma decursinol concentrations compared with decursinol-treated group in rats. On the basis of these results, it is concluded that co-existing substances or co-administration with other drugs may influence the plasma levels of decurisinol after oral administration.

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A Mass Preparation Method of (+)-Decursinol from the Roots of Angelica gigas (참당귀(Angelica gigas) 뿌리 추출물로부터 (+)-Decursinol의 대량 제조 방법)

  • Lee, Jee-Hyun;Choi, Yong-Seok;Kim, Joo-Hwan;Jeong, Hye-Gwang;Kim, Dong-Hee;Yun, Mi-Yong;Kim, Jin-Sook;Lee, Sang-Han;Cho, Soo-Hyun;Shen, Gui-Nan;Kim, Eui-Geom;Jin, Wen-Yi;Song, Gyu-Yong
    • YAKHAK HOEJI
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    • v.50 no.3
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    • pp.172-176
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    • 2006
  • The purpose of this study is to develop a mass preparation method of (+)-decursinol from Angelica gigas roots. Recently; it has been shown that (+)-decursin, (+)-decursinol angelate and its analogues, isolated from Angelica gigas roots, exhibit various biological activities such as antitumor, antibacterial and neuroprotective activities. The contents of these compounds, ester form of (+)-decursinol, is very high in the Angelica gigas roots, whereas the content of (+)-decursinol itself is very low Therefore, (+)-decursinol which can be used as starting material to synthesize various its analogues was easily prepared from decursin analogues in the Angelica gigas roots. In order to achieve such aim, the Ether-Fr. of the ethanol exact of Angelica gigas roots was hydrolysed with various alkalis and solvents. As a result, the order of (+)-decursinol preparation was 1) NaOH, KOH, 2) $K_2CO_3$, and 3) $NaHCO_3$ as alkali. Also, the yield of (+)-decursinol was higher in diethyl ether than any other solvent conditions. From 1 kg of dried Angelica gigas roots, we could obtain 27.4 g of (+)-decursinol as a pure white solid.

Pharmacokinetic Study of Decursinol Following Oral Administration in Rat (데커시놀추출물의 경구투여후 흰쥐에 있어서의 약물속도론적 연구)

  • Kim, Ji-Hae;Choi, Song-Am;Kim, Dong-Chool
    • Journal of Pharmaceutical Investigation
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    • v.33 no.3
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    • pp.195-199
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    • 2003
  • The objective of this study is to investigate the pharamacokinetic parameters of decursinol following oral administration in Sprague-Dawley rats. The plasma concentration of decursinol was determined by LC/MS with APCI positive mode. The m/z value of decursinol was observed at 247. Following oral administration of decursinol extract, the apparent clearance was $5.3{\pm}2.7\;ml/hr/rat$, the absorption half life was $2.5{\pm}0.41\;hr$, the elimination half life was $3.05{\pm}1.57\;hr$, and the apparent volume of distribution was $21{\pm}12\;ml/rat$. The LC/MS method was successfully applied to the pharmacokinetic study of decursinol.

Effects of Decursin and Decursinol on the Germination and Growth of Plants (Decursin과 Decursinol이 식물의 생장과 발아에 미치는 영향)

  • 이진범
    • Journal of Plant Biology
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    • v.19 no.1
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    • pp.7-13
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    • 1976
  • Biological activities of decursin and decursinol, natural coumarin derivatives, on the germination, growth and adventitious root formation of several plants were observed. In 10 ppm of decursin or decursinol, the growth of Avena coleoptile sections was inhibited, and the activity of IAA-oxidase was gradually enhanced by the increase of its concentrations. Inhibition effect on seed germination was observed from 100 ppm of each chemcials, and the activity of amylase in the germinating seeds was also gradually decreased. However, in the higher concentrations of decursin, the inhibited germination rate of wheat was slightly reduced. Decursin and decursinol also promoted the adventitious root formation in seeds of Phaseolus vulgaris.

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Studies on the Coumarin Constituents of the Root of Angelica gigas Nakai -Isolation of decursinol angelate and assay of decursinol angelate and decursin- (당귀(當歸)의 Coumarin 성분연구(成分硏究) -Decurson의 구조이성체 Decursinol Angelate의 분리(分離) 및 정량(定量)-)

  • Ryu, Kyung-Soo;Hong, Nam-Doo;Kim, Nam-Jae;Kong, Young-Yun
    • Korean Journal of Pharmacognosy
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    • v.21 no.1
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    • pp.64-68
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    • 1990
  • From the root of Angelica gigas Nakai, decursinol angelate was isolated, which is structural isomer of decursin. Raw material contained average 2.44% of decursinol angelate and 3.30% of decursin by the GC analysis.

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Studies on the Components of Umbelliferae Plants in Korea -Pharmacological Study of Decursin, Decursinol and Nodakenin- (한국산 산형과 식물의 성분연구 -Decursin, Decursinol 및 Nodakenin 의 일반약리작용-)

  • Chi, Hyung-Joon;Kim, Hak-Sung
    • Korean Journal of Pharmacognosy
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    • v.1 no.1
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    • pp.25-32
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    • 1970
  • Decursin, decursinol and nodakenin which were isolated from the root of Angelica gigas $N_{AKAI}$ (Umbelliferae) that is used as a botanical drug Dang-Gui (當歸) in Korea show following general pharmacologic activities. Decursin and decursinol increased the motility of the excised duodenum of the rabbit, but nodakenin did not. All of these three compounds depressed the heart contraction of the frog and blood pressure of the carotid artery and respiration of the rabbit. Decursin increased uterus contraction of the rabbit, but decursinol depressed it. And the tonus of the muscle of the earth worm was increased by decursinol, but was decreased by decursin.

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Protective Effect of Decursinol on Mouse Models of Sepsis: Enhancement of Interleukin-10

  • Jung, Jun-Sub;Yan, Ji-Jing;Song, Dong-Keun
    • The Korean Journal of Physiology and Pharmacology
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    • v.12 no.2
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    • pp.79-81
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    • 2008
  • The effects of decursinol on various models of sepsis were investigated. Intra-peritoneal pretreatment of mice with various doses of decursinol ($1{\sim}100$ mg/kg) effectively suppressed lethality induced in three mouse models of experimental sepsis, i.e., lipopolysaccharide (LPS)/D-galactosamine (GalN), high-dose LPS (20 mg/kg), and cecal ligation and puncture (CLP). Intra-peritoneal pretreatment of mice with decursinol (50 mg/kg) markedly enhanced the LPS/GalN -induced increase of plasma interleukin-10 (IL-10) levels, without affecting plasma TNF-${\alpha}$, IL-6 and IL-12 levels. These results suggest that decursinol could be effective for prevention or treatment of sepsis.

Validations of Analysis Methods for Decursin and Decursinol Angelate of Angelicae gigantis Radix by Reversed-Phase Liquid Chromatography

  • Lee, Jong-Pill;Chang, Seung-Yeup;Park, Sang-Yong
    • Natural Product Sciences
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    • v.10 no.6
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    • pp.262-267
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    • 2004
  • A reversed-phase liquid chromatographic method for decursin and decursinol angelate of Angelicae gigantis Radix, an important crude drug in Korean traditional medicine, was developed and validated. Decursin and decursinol angelate, the structure isomer (pyranocoumarin) each other, are the main organic constituents in Angelicae gigantis Radix. This method was developed using a RP-18 column, UV detector at 280 nm and 50% acetonitrile solution containing 0.01 M sodium dodecyl sulfate and 25 mM sodium dihydrogen phosphate (pH 5.0) as the mobile phase. Various validation parameters were included and evaluated satisfactorily. Linearity was established in range 2-75 mg/ml of decursin and decursinol angelate (correlation coefficient = 0.9997 and 0.9995, respectively). This analytical method showed good accuracy (98.1% and 99.5%, respectively). Precision (repeatability) revealed a relative standard deviation value of 1.71% (decursin) and 3.19% (decursinol angelate). For intermediate precision measure the considered variables were equipment and days. A robustness test showing the influence of deferent counter-ion concentration in mobile phase was also performed.

Pharmacological Effect of Decursin and Decursinol Angelate from Angelica gigas Nakai (신약 개발을 위한 참당귀(Angelica gigas Nakai) 추출 Decursin과 Decursinol Angelate의 약리 작용)

  • Son, Chu-Young;Baek, In-Hwan;Song, Gyu-Yong;Kang, Jae-Seon;Kwon, Kwang-Il
    • YAKHAK HOEJI
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    • v.53 no.6
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    • pp.303-313
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    • 2009
  • Traditionally, Cham dang-gui (Angelica gigas Nakai) is one of the most popular herbal medicines in Asian countries including Korea. A. gigas has been used as a functional food product for treatment anemia, women's health care, a sedative, an anodyne or a tonic agent. Decursin and decursinol angelate isolated from the roots of A. gigas are pyranocoumarin compounds. Recently, as the global herbal medication market is increasing, investigations about pharmacological effects of decursin and decursinol angelate are rapidly increasing. We summarized previous studies about pharmacological effects of decursin and decursinol angelate, and reviewed relation with pharmacological effects of decursin and decursinol angelate on human disorder, focused on the approach for new drug development. Pharmacological effects of decursin and decursinol angelate were classified as anti-tumor activity, anti-bacterial activity, improvements of the circulating system, inhibition of cytochrome P-450 activity, anti-inflammation activity, anti-oxidant activity and cognitive-enhancing activites. The activity of A. gigas with improvement of the circulating system may have wide therapeutic potential for circulatory diseases, including diabetes, hyperlipidemia and atherosclerosis. Also, anti-inflammation activity A. gigas may be beneficial for the treatment and prevention of asthma, atopic dermatitis and rheumatism arthritis. This relation could potentially lead to the development of herbal new drugs. In order to development a new drug containing decursin and decursinol angelate, it is also necessary to consider the safety profile, and the information in this review would contribute to development a new drug from herbal medicine.