• Title, Summary, Keyword: development

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Geft is dispensable for the development of the second heart field

  • Fan, Xiongwei;Hou, Ning;Fan, Kaiji;Yuan, Jiajia;Mo, Xiaoyang;Deng, Yun;Wan, Yongqi;Teng, Yan;Yang, Xiao;Wu, Xiushan
    • BMB Reports
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    • v.45 no.3
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    • pp.153-158
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    • 2012
  • Geft is a guanine nucleotide exchange factor, which can specifically activate Rho family of small GTPase by catalyzing the exchange of bound GDP for GTP. Geft is highly expressed in the excitable tissue as heart and skeletal muscle and plays important roles in many cellular processes, such as cell proliferation, migration, and cell fate decision. However, the in vivo role of Geft remains unknown. Here, we generated a Geft conditional knockout mouse by flanking exons 5-17 of Geft with loxP sites. Cre-mediated deletion of the Geft gene in heart using Mef2c-Cre transgenic mice resulted in a dramatic decrease of Geft expression. Geft knockout mice develop normally and exhibit no discernable phenotype, suggesting Geft is dispensable for the development of the second heart field in mouse. The Geft conditional knockout mouse will be a valuable genetic tool for uncovering the in vivo roles of Geft during development and in adult homeostasis.

ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits AP-1- and SRE-mediated transcriptional activity

  • Deng, Yun;Liu, Bisheng;Fan, Xiongwei;Wang, Yuequn;Tang, Ming;Mo, Xiaoyang;Li, Yongqing;Ying, Zaochu;Wan, Yongqi;Luo, Na;Zhou, Junmei;Wu, Xiushan;Yuan, Wuzhou
    • BMB Reports
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    • v.43 no.3
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    • pp.193-198
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    • 2010
  • In this study, we report the identification and characterization of a novel C2H2 zinc finger protein, ZNF552, from a human embryonic heart cDNA library. ZNF552 is composed of three exons and two introns and maps to chromosome 19q13.43. The cDNA of ZNF552 is 2.3 kb, encoding 407 amino acids with an amino-terminal KRAB domain and seven carboxyl-terminal C2H2 zinc finger motifs in the nucleus and cytoplasm. Northern blotting analysis indicated that a 2.3 kb transcript specific for ZNF552 was expressed in liver, lung, spleen, testis and kidney, especially with a higher level in the lung and testis in human adult tissues. Reporter gene assays showed that ZNF552 was a transcriptional repressor, and overexpression of ZNF552 in the COS-7 cells inhibited the transcriptional activities of AP-1 and SRE, which could be relieved through RNAi analysis. Deletion studies showed that the KRAB domain of ZNF552 may be involved in this inhibition.

KBTBD7, a novel human BTB-kelch protein, activates transcriptional activities of SRE and AP-1

  • Hu, Junjian;Yuan, Wuzhou;Tang, Ming;Wang, Yuequn;Fan, Xiongwei;Mo, Xiaoyang;Li, Yongqing;Ying, Zaochu;Wan, Yongqi;Ocorr, Karen;Bodmer, Rolf;Deng, Yun;Wu, Xiushan
    • BMB Reports
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    • v.43 no.1
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    • pp.17-22
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    • 2010
  • In this study, a novel member of BTB-kelch proteins, named KBTBD7, was cloned from a human embryonic heart cDNA library. The cDNA of KBTBD7 is 3,008 bp long and encodes a protein product of 684 amino acids (77.2 kD). This protein is highly conserved in evolution across different species. Western blot analysis indicates that a 77 kD protein specific for KBTBD7 is wildly expressed in all embryonic tissues examined. In COS-7 cells, KBTBD7 proteins are localized to the cytoplasm. KBTBD7 is a transcription activator when fused to GAL4 DNA-binding domain. Deletion analysis indicates that the BTB domain and kelch repeat motif are main regions for transcriptional activation. Overexpression of KBTBD7 in MCF-7 cells activates the transcriptional activities of activator protein-1 (AP-1) and serum response element (SRE), which can be relieved by siRNA. These results suggest that KBTBD7 proteins may act as a new transcriptional activator in mitogen-activated protein kinase (MAPK) signaling.

The Marriage Stewardship and the Role and Status of Social Development Network in Singapore (결혼 책임주의와 싱가포르 Social Development Network의 역할과 위상)

  • Kim, Myung-Hee
    • Journal of the Korea Convergence Society
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    • v.9 no.1
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    • pp.143-149
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    • 2018
  • This paper focuses on how Singapore has adopted the 'Marriage Promotion Policy' for resolving the low birthrate and how it has been using the Social Development Network (SDN) to cope with the problem of low fertility. Social Development Network is an organization that shows Singapore's national stewardship to marriage, and has developed from the beginning to the social phenomenon of increasing number of highly educated women and decreasing fertility. The predecessor of Social Development Network is 'Social Development Unit' (SDU), absorbed the 'Social Development Service' (SDS) that was launched later, and reborn as Social Development Network in 2009. At its inception, Social Development Network has been recruiting and organizing its own memberships without the help of commercial marriage agencies, and since 2006 has been dedicated to delegating dating and matchmaking services to private companies and supporting projects. The purpose of this paper using literature analysis is to analyze the status and role of Social Development Network from the theoretical point of view of marital stewardship to the present, and to suggest meaningful implications for Korea which has the same social experience.

Software Development Effort Estimation for Testing Data Analysis (테스팅 데이터 분석을 통한 소프트웨어 개발 노력 추정)

  • Jung, Hye-Jung;Yang, Hae-Sool
    • The KIPS Transactions:PartD
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    • v.11D no.1
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    • pp.173-182
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    • 2004
  • The research to estimate development effort of software has been progress. But, it is not easy gain that testing data for estimating of development effort. Also, if we get the testing data, it is important that analysis testing data. In this paper, we study the data analysis of software development effort using the 789 software development projects which developed in the 1990's. Software development scale and software development team site are various. Using the characteristic of factor, we have to study characteristic of data and we estimate the development effort step by step. First, we prove the difference of development effort with the 789 project data according to development type, development environment, the development language etc. Also, we execute the crosstabs analysis that team site and function point.

De Novo Assembly and Comparative Analysis of the Enterococcus faecalis Genome (KACC 91532) from a Korean Neonate

  • Ham, Jun Sang;Kwak, Woori;Chang, Oun Ki;Han, Gi Sung;Jeong, Seok Geun;Seol, Kuk Hwan;Kim, Hyoun Wook;Kang, Geun Ho;Park, Beom Young;Lee, Hyun-Jeong;Kim, Jong Geun;Kim, Kyu-Won;Sung, Samsun;Lee, Taeheon;Cho, Seoae;Kim, Heebal
    • Journal of Microbiology and Biotechnology
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    • v.23 no.7
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    • pp.966-973
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    • 2013
  • Using a newly constructed de novo assembly pipeline, finished genome level assembly had been conducted for the probiotic candidate strain E. faecalis KACC 91532 isolated from a stool samples of Korean neonates. Our gene prediction identified 3,061 genes in the assembled genome of the strain. Among these, nine genes were specific only for the E. faecalis KACC 91532, compared with all of the four known reference genomes (EF62, D32, V583, OG1RF). We identified genes related to phenotypic characters and detected E. faecalis KACC 91532-specific evolutionarily accelerated genes using dN/dS analysis. From these results, we found the potential risk of KACC 91532 as a useful probiotic strain and identified some candidate genetic variations that could affect the function of enzymes.

A Preliminary Study on the Development of Infant-Toddler Developmental Scale : 1 to 36 Months (영유아발달 척도의 개발을 위한 기초 연구: 1-36개월 영유아를 중심으로)

  • Song, Jin-Suk
    • Korean Journal of Human Ecology
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    • v.9 no.4
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    • pp.405-419
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    • 2000
  • The purpose of this study was to develop a Korean infant-toddler developmental scale and to examine the demographic variables and infant-toddler development. The subjects were 167 mothers who have infant and toddler from 1 to 36 months of age in Seoul, Daejeon, Chungju, Yeasan, and llsan in Korea. The subscales consisted of language development, social and emotional development, physical development, and cognitive development. The significant variables which are influenced the infant-toddler development were age of infant-toddler and age of father.

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Abnormal oceanic conditions around the Korean peninsula caused by typhoons

  • Suh, Young-Sang;Gu, Ji-Young;Hwang, Jae-Dong;Lee, Na-Kyung;Jang, Lee-Hyun;Jeon, Kyoung-Ok
    • Proceedings of the KSRS Conference
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    • pp.640-641
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    • 2003
  • Abnormal oceanic conditions associated with the passage of typhoons are examined using hydrographic and satellite data 1990-2002. During the passage period of typhoons in the Korean waters, an abrupt decrease of sea surface temperature(SST) in range of 5 to 8 $^{\circ}$ was observed. The areas of SST decrease were an order of 100-200km, and the low SST lasted about 15-25 days after passage of typhoon. After passage of typhoon, the water temperatures in the surface mixed layer of 30m show negative anomalies for quite a long period. In addition, stratification parameters were substantially decreased and chlorophyll a density was rapidly increased.

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