• Title, Summary, Keyword: egg yolk antibody

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Relationship of Antibodies in Egg Yolk and Serum against Major Antigens of Bacterial Agents in Porcine Atrophic Rhinitis, Pneumonic Pasteurellosis and Pleuropneumonia (돼지 위축성 비염, 파스튜렐라성 폐렴 및 흉막폐렴 원인균의 주요 항원에 대한 IgG 와 IgY 의 상관 관계 분석)

  • Shin, Na-Ri;Kim, Jong-Man;Yoo, Han-Sang
    • Korean Journal of Veterinary Research
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    • v.42 no.3
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    • pp.371-376
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    • 2002
  • Swine respiratory diseases have induced severe economic lasses in swine industry worldwide. Therefore, several methods have been made and applied to prevent and control the diseases. However, these methods still have a problem and also induce side effects. Recently, the use of egg yolk antibody was introduced to control and prevent the diseases as one of new trials. As a study of using egg yolk antibody, antibody titers against several different antigens of major pathogens in swine respiratory diseases were compared in egg yolk and serum of hens immunized with those antigens. The titers were measured by ELISA using the antigens as coating antigens. The relationship in antibody titers between egg yolk and serum were identified by analysis of variance for linear regression. Almost of antigens used in this study showed the high relationship in antibody titers between egg yolk and serum (r = 0.87 ~ 0.93) even though the relationship in antibody titers against P. multocida A:3 IROMP was slightly low (r = 0.74)(P<0.01). These results indicated that antibody titer in egg yolk could be useful to predict the titer in serum of chicken.

Immune Status of Breeding Hens Against Newcastle Disease (종계군(種鷄群)의 Newcastle Disease에 대(對)한 면역상태(免疫狀態)에 관(關)한 연구(硏究))

  • Yeo, Sang Geon;Choi, Won Pil
    • Korean Journal of Veterinary Research
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    • v.19 no.1
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    • pp.45-51
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    • 1979
  • The present study was undertaken to observe the immune status of breeding hens and laying hens against Newcastle disease (ND). The methods of extraction of hemagglutination inhibition (HI) antibody from egg yolk, the detection of HI antibody in egg albumen and the correlation between HI antibody titers in maternal sera and egg yolks were discussed. For the purposes of these experiments, 9 flocks of breeding hens and 16 flocks of laying hens immunized against Newcastle disease virus were investigated. The vaccination program of tested flocks was 3-3-3 or 4-4-4 in general. The results obtained are summerized as follows: Freezing-thawing was the best method far antibody extraction from egg yolk for HI test. The HI antibody against NDV was found in egg albumen (geometric mean, 4.5), but lower than that found in egg yolk (32.1). The geometric mean of HI antibody titers of egg yolks (84.1) was higher than that of maternal sera (68.4) and day-old chicken sera (25.3). There was correlation between HI antibody titers of maternal sera(Y) and those of egg yolks(X). The coefficient correlation was r=0.63, and the line of regression of Y on X was $\hat{Y}$=35.91+0.35X.

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Production of Egg Yolk Antibody (IgY) Against Human Placental DNA-Dependent RNA Polymerase II

  • Lee, Yoon-Ik;Surzycki, Stefan S.;Lee, Young-Ik
    • BMB Reports
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    • v.28 no.1
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    • pp.27-32
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    • 1995
  • Polyclonal antibodies against human DNA-dependent RNA polymerase II (HPP II) were generated from chicken egg yolk after immunization with RNA polymerase II as an antigen. The antibodies from egg yolk (IgY) were purified and characterized. IgY showed a specificity against DNA-dependent RNA polymerase II, and was a polyclonal antibody against 12 subunits of polymerase II. An amount of 0.35 mg of IgY was obtained freman HPP II-Sepharose affinity column using 10 eggs from a chicken immunized against RNA polymerase II as an antigen. These antibodies can be used for isolating the genes for RNA polymerase II components, and for in vitro transcription assays using HP-RNA polymerase II.

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Development of preventive method for enterotoxigenic colibacillosis using egg yolk antibodies I. Imune responses of hens immunized against combined Escherichia coli pilus antigens and heat labile toxin (난황항체를 이용한 돼지 대장균 설사증 방제기법 개발 I. 대장균 pilus 항원과 LT로 면역시킨 닭의 면역반응)

  • Woo, Seng-ryong;Kim, Jong-man;Kwon, Chang-hee;Lee, Hee-su;Lym, Suk-kyoung;Kim, Jong-yeom
    • Korean Journal of Veterinary Research
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    • v.38 no.4
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    • pp.829-836
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    • 1998
  • Immunogenicity of Escherichia coli pilus and LT were evaluated in 20-week-old hens. The antigens were consisted of K88, K99, 987p pilus and heat labile toxin purified from enterotoxigenic Escherichia coli. The durations of antibody titers in sera and egg yolk were investigated by an enzyme-linked immunosorbent assay(ELISA). After first inoculation, antibody titers in sera reached at peak 2 weeks postinoculation. However, peak antibody titers in egg yolk were detected 4 weeks postinoculation, indicating that transfer of immunoglobulin from serum to egg yolk took about two weeks period. Although there were slight reduction in titers, the specific antibodies in egg yolk lasted up to 3 months. Immune responses against monovalent and combined antigens were showed as almost same patterns. The transfer rate of antibodies from serum to egg yolk didn't show any significant differences among three pilus antigens in this study. Considering the concentrations of antigens in each inoculated group, multivalent antigens containing heat labile toxin of E coli were found to be more immunogenic than monovalent antigen in producing specific antibodies. From this experiment, it was demonstrated that multivalent antigens containing three pilus and heat labile toxin could be a promising candidate for the production of egg yolk antibodies for prophylactic use in preventing swine colibacillosis in future.

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Salmonella enteritidis의 편모항원에 대한 난황항체의 ,생산 x Production of Egg Yolk Antibodies against Flagella Antigen of Salmonella enteritidis

  • 김정우
    • Korean Journal of Poultry Science
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    • v.25 no.4
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    • pp.161-167
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    • 1998
  • This experiment was carried out to develope the production of specific yolk antibody from laying hens immunized with antigens from Salmonella enteritidis. Antigenic protein isolated from the flagella of Salmonella enteritidis, determined by SDS-PAGE, was pure and has a molecular mass of approximately 54.6 kDa. It was observed that the antibody titers both in egg yolk and serum were performed at 2 weeks after immunization with flagella antigen to the laying hen. And the level was increased gradually to 6 weeks after immunization. At the time of 6 weeks, the antibody titer of yolk showed higher than that of serum. According to the results of specificity test(ELISA), the yolk antibody did not react with different bacterial strains(S. choleraesuis, ETEC Kl2:K99, K88,987P), but reacted only with S. enteritidis strain. The contents of immunoglobulin(IgY) in an egg yolk was 106mg approximately. By the isolation procedure of IgY from the egg yolk, 88.3 percent of IgY content was recovered in this study.

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The Level of Immune Response on Bovine Serum Albumin(BSA) Injection to Different Breeds of Laying Hen (Bovine Serum Albumin 투여가 산란계의 품종별 면역반응에 미치는 영향)

  • 채현석;김동운;안종남;김용곤;이종문;노환국;윤병선;심정석
    • Korean Journal of Poultry Science
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    • v.26 no.4
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    • pp.247-252
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    • 1999
  • This experiment was carried out to investigate the effects of the laying hens on the immune response against bovine serum albumin(BSA) in egg yolk. Total 45 laying hens were divided into three groups according to breeds (White Leghorn, ISA Brown, Native hen). They were fed the experimental diet for 12 weeks. Immune response were examind in egg yolk from three groups of hens injected with BSA. The results obtained from this work were summaried as follows : 1. The weight of egg yolk and the percentage of hen-day production in the ISA Brown hens are greater than those in the Native hens and the White Leghons. 2. IgY concentrations in eggs from hens immunized with BSA were not different among the breeds laying hens. 3. The anti-BSA antibody activities determined by enzyme linked immunosorbent assay (ELISA) in the egg yolk were similar between the White-Leghorn and ISA Brown hens, but Native hens tended to decrease in 20∼50 days respectively. Therefore, the weight of egg yolk and the percentage of hen-day production in the ISA Brown hens are greater than those in the Native hens and the White Leghons will be as important factors for an efficient production of IgY.

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Control of canine respiratory and diarrheal disease using egg yolk antibodies I. Induction of antibody in hens immunized with combined antigens of Bordetella bronchiseptica, parvovirus and canine distempervirus (난황면역제를 이용한 개 주요 소화기 및 호흡기질병 방제에 관한 연구 I. 개 보데텔라, 파보바이러스 및 개디스템퍼 항원의 닭에서의 면역반응)

  • Lee, Hee-soo;Kim, Jong-man;Woo, Seng-ryong;Jung, Byeong-yeal;Cho, Yun-Sang;Tark, Dong-seob;Lim, Sook-kyoung;Yoo, Han sang;Yoon, Yong-dhuk;Huh, Woo;Mun, Young-sik;Oh, Jin-sik
    • Korean Journal of Veterinary Research
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    • v.44 no.1
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    • pp.65-71
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    • 2004
  • This study was carried out to produce IgY against B. bronchiseptica, parvovirus and distemper virus that are major pathogens in alimentary and/or respiratory diseases of dogs. In the comparison of adjuvants, ISA70 was the best in the rapid induction and maintence of antibody titers. Agglutination antibody titers against B. bronchiseptica were 1:1,280 ~ 1:10,240 in sera and 1:160 ~ 1:1,280 in egg yolk. Hemagglutination inhibition(HI) titers against parvovirus in sera and egg yolk were 1:80 ~ 1:320 and 1:64 ~ 1:256, respectively. Virus neutralization titers against canine distemper was 1:8 ~ 1:64 in sera and egg yolk. These results suggested that egg yolk antibody titers could be variable according to a sort of adjuvant and antigens of the pathogens.

Egg Yolk Antibody and Its Application

  • Kim, Mujo;Shinji Higashiguchi;Yoshitomo Iwamoto;Yang, Han-Chul;Cho, hong-Yon;Hsjime Hatta
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.2
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    • pp.79-83
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    • 2000
  • A han transfers her serum immunoglobulin G to the agg (IgY) and gives immunity to her offspring. Therefore, The hen agg can be an effective supplier of a large amount of antigen specific antibody that accumulates in the egg yolk. Antigen specific antibody has been widely used for immunological analysis in the field of diagnosis as well as pure scientific research. The production and separation technology of IgY is demonstrated in the present study.

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Development of preventive method for enterotoxigenic colibacillosis using egg yolk antibodies II. Therapeutic effect of egg yolk antibodies against colibacillosis of piglets (난황항체를 이용한 돼지 대장균설사증 방제기법 개발 II. 난황항체의 돼지 대장균증에 대한 치료효과)

  • Kim, Jong-man;Woo, Seng-ryong;Kweon, Chang-hee;Kim, Jong-yeom;Huh, Won
    • Korean Journal of Veterinary Research
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    • v.38 no.4
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    • pp.837-842
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    • 1998
  • The present study describes the effectiveness of egg yolk antibodies (IgY) against enteric colibacillosis and edema disease in piglets. The antibodies were gained from the egg yolk of hens immunized with k88, k99, 987p fimbrial adhesin and heat-labile toxin antigens of enterotoxigenic Escherichia coli (ETEC). Orally-administered egg yolk antibodies solution protected against experimental challenge with ETEC $K88^+$ and $k99^+$ strains in neonatal piglets and mice. In field trial, a total of 598 diarrheal piglets were orally treated with 3ml of antibody once a day to determine for the therapeutic effect. Of them, 582 (97.3%) piglets were recovered from diarrhea in 3 days. We also experimentally treated with the egg yolk antibodies twice a day for 5 consecutive days for 94 weaning piglets with edema disease for the determination of therapeutic effects. Seventy four piglets (78.7%) were recovered from clinical edema signs. Theses findings indicate that egg yolk antibodies against k88, k99, 987p and LT of ETEC are useful source of passive immunity for enteric colibacillosis and edema disease of piglets.

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Control of swine respiratory disease using egg yolk antibodies I. Analysis of immunogenes of Bordetella bronchiseptica, Pasteurella multocida and Actinobacillus pleuropneumoniae and production of IgY (난황항체를 이용한 돼지 호흡기 질병 방제 관한 연구 I. Bordetella bronchiseptica, Pasteurella multocida 및 Actinobacillus pleuropneumoniae의 주요 면역원 분석 및 IgY의 생산)

  • Shin, Na-ri;Kim, Jong-man;Yoo, Han-sang
    • Korean Journal of Veterinary Research
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    • v.40 no.3
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    • pp.551-561
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    • 2000
  • Swine respiratory diseases have induced severe economic losses in swine industry worldwide. Several methods have been developed and applied to prevent and control the disease. However, those are still problematic in swine industry. Recently, the use of egg yolk antibodies with several advantages was introduced and applied to control diseases in animal as well as human. As the first step of the use of egg yolk antibodies in the control of the swine respiratory diseases, we investigated the immunogens of the causative agensts of the diseases and immune response in egg yolk of hens immunized with them. Bacterial antigens prepared from Bordetella bronchiseptica, Pasteurella multocida 3A and 4D, and Actinobacillus pleuropneumaniae serotype 2 and 5 were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blot and toxicity test in mice. The antigens were injected into laying hens in order to produce antibodies against them in egg yolk. After chickens were immunized three times in 2 weeks interval, the profile of antibody production was examined by ELISA. The production of antibody in egg yolk was started in 2 weeks after the first injection, reached peak in 6-8 weeks and maintained until 12 weeks. Of two adjuvants used in this study, ISA70 was more effective than aluminum hydroxide gel in enhancing immunogenecity, laying rates and safety in hens. These results suggested that egg yolk antibodies could be a good source for production of antibodies specific to pathogenic bacteria inducing respiratory diseases of swine.

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