• Title, Summary, Keyword: endocrine cell

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Effects of zinc and resveratrol on cadmium-induced apoptosis and cell arrest in MCF-7 and MDA-MB-231 cells

  • Lee, Su-Jung;Ho, Shin-Jae;Kim, Tae-Sung;Moon, Hyun-Ju;Kang, Il-Hyun;Kim, In-Young;Oh, Ji-Young;Lee, Young-Ju;Kim, An-Keun;Han, Soon-Young
    • Proceedings of the PSK Conference
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    • pp.115.1-115
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    • 2003
  • Cadmium, a human carcinogen, can induce apoptosis in various cell lines. Despite extensive research, the mechanisms of cadmium-induced apoptosis are poorly understood, and its toxicity and estrogenic potential in human are not clear. This study was performed to investigate the apoptotic activities of cadmium on two human breast cancer cell lines: MCF-7 cells, an estrogen receptor (ER) positive cell line, and MDA-MB-231 cells, an ER negative cell line. Both cells were treated with $CdCl_2$ 100$\mu$M for 12hrs, and the spoptosis was determined by DNA fragmentation, DAPI staining, and expression of caspase-9. (omitted)

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Cadmium Induces Cell Cycle Arrest and Change in Expression of Cell Cycle Related Proteins in Breast Cancer Cell Lines

  • Lee Young Joo;Kang Tae Seok;Kim Tae Sung;Moon Hyun Ju;Kang Il Hyun;Oh Ji Young;Kwon Hoonjeong;Han Soon Young
    • Toxicological Research
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    • v.21 no.1
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    • pp.77-85
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    • 2005
  • Cadmium is an environmental pollutant exposed from contaminated foods or cigarette smoking and known to cause oxidative damage in organs. We investigated the cadmium-induced apoptosis and cell arrest in human breast cancer cells, MCF-7 cells and MDA-MB-231 cells. Obvious apoptotic cell death was shown in CdCl₂ 100 μM treatment for 12 hr, which were determined by DAPI staining and flow cytometric analysis. In cell cycle analysis, MCF-7 cells and MDA-MB-231 cells were arrested in S phase and G2/M phase respectively. These could be explained by the induction of cell cycle inhibitory protein, p21/sup Waf1/Cip1/ and p27/sup Kip1/, expression and reduction of cyclin/Cdk complexes in both cell lines. The decreased expression of cyclin A and Cdk2 in MCF-7 cells and cyclin B1 and Cdc2 in MDA-MB-231 cells were consistent with the flow cytometric observation. p-ERK expression was increased dose-dependent manner in both cell lines. It suggests that ERK MAPK pathway are involved in cadmium-induced cell cycle arrest and apoptosis. Moreover, cotreatment of zinc (100 μM, 12 hr) recovered the cadmium-induced cell arrest in both cells, which shows cadmium-induced oxidative stress mediates apoptosis and cell cycle arrest in human breast cancer cells.

Ultrastructure of Endocrine Cells in the Midgut Epithelium of the Japanese Cockroach, Periplaneta japonica (집바퀴의 중장 상피조직내에 있는 내분비세포의 미세구조)

  • Yu, Chai-Hyeock
    • Applied Microscopy
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    • v.26 no.4
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    • pp.421-430
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    • 1996
  • The endocrine cells in the midgut epithelium of the Japanese cockroach, Periplaneta japonica were observed by the light and electron microscopy. The midgut epithelium of the last instar larva and adult cockroach consisted of principal columnar cells, regenerative cells, and secretory granular cells. Midgut endocrine cells were positioned basally as a cone-shaped single cell in the epithelium or underneath the regenerative crypt cells. When midgut epithelium grows and the cell composing it transforms, between the endocrine cells and regenerative cells were made desmosome type junction and large vesicular shaped stretches of loose contact. The endocrine cells were characterized by a clear cytoplasm with abundant Golgi complex and numerous secretory granules. The secretory granules in the cell were spherical and electron dense with their diameter of $200{\sim}400nm$. The secretory granules have been observed as discharged by exocytosis on the basal and lateral side of the cell.

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An histological and immunohistochemical study of endocrine cells in the gastrointestinal tract of the Amur lizard (Takydromus amurensis) (아무르장지뱀의 위장관 내분비세포에 관한 조직화학적 및 면역조직화학적 연구)

  • Lee, Hyeung-sik;Lee, Mal-soon;Lee, Jae-hyun
    • Korean Journal of Veterinary Research
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    • v.35 no.1
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    • pp.67-73
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    • 1995
  • The distribution and the frequency of endocrine cells in the GIT of the Amur lizard(Takydromus amurensis) were investigated using silver techniques and immunohistochemical method. Only argyrophil cells stained by the Grimelius technique were found numerous in the pyloric glands and moderate in the duodenum. Two types of immunoreactive endocrine cells were identified by immunohistochemical method. Bovine CG-immunoreactive cells were demonstrated the entire GIT. BPP-immunoreactive cells were restricted in the duodenum and the ileum. The results showed that: the number of argyrophil cells was lower than the number of cells stained with bovine CG antiserum. Therefore, bovine CG-immunostaining and the Grimelius silver technique did not correspond with various endocrine cells in the Amur lizard.

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Immunohistochemistry of the Pancreatic Endocrine Cells of the Red-eared Slider (Trachemys scripta elegans)

  • Ku, Sae-Kwang;Lee, Hyeung-Sik;Lee, Jae-Hyun;Park, Ki-Dae
    • Animal cells and systems
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    • v.4 no.2
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    • pp.187-193
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    • 2000
  • Regional distribution and relative frequency of endocrine cells in the pancreas of the red-eared slider, Trachemys scripta elegans, were investigated by immunohistochemical methods. Chromogranin (Cg) A-, serotonin-, insulin-, glucagon-, somatostatin-, bovine pancreatic polypeptide (BPP)- and human pancreatic polypeptede (HPP)-immunoreactive cells were identified in this study. Most of immunoreactive cells in the exocrine and endocrine pancreas (Langerhans islet) were generally spherical or spindle-shaped (open-typed cell), while occasionally cells round in shape (close-typed cell) were found in the basal portion or interepithelial regions of the pancreatic duct. These immunoreactive cells were located in the exocrine, endocrine pancreas and/or basal or interepithelial portion of the pancreatic duct. Serotonin-immunoreactive cells were found in the basal portion of epithelia of the pancreatic duct at a low frequency and interacinar region of the exocrine at a moderate frequency. Insulin-immunoreactive cells were found in the central portion of the endocrine pancreas, interacinar regions of the exocrine pancreas and basal portion of the epithelia of the pancreatic duct at high, moderate and low frequencies, respectively. Glucagon-immunoreactive cells were detected in the periphery of the endocrine pancreas, interacinar region of the exocrine pancreas and basal portion of the epithelia or interepithelia of the pancreatic duct at high, moderate and moderate frequencies, respectively. Somatostatin-immunoreactive cells were dispersed in the whole area of the endocrine pancreas, interacinar regions of exocrine pancreas and basal portion of the epithelia or interepithelia of the pancreatic duct at a moderate frequency. BPP- and HPP-immunoreactive cells were detected in the iinteracinar region of the exocrine pancreas at moderate and hige frequencies, respectively. However, no Cg A- and motilin-immunoreactive cells were detected in this study.

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An electron microscopic study on gastro-enteroendocrine cells of frog (Rana dybowskii) (산개구리 위장관 내분비세포의 전자현미경적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.30 no.2
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    • pp.129-143
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    • 1990
  • In the present paper, the distribution, relative frequences and cell types of endocrine cells in the gastrointestinal tract of the frog (Rana dybowskii) during the hibernating and the active phase were examined by light and electron microscopy. The results obtained are summarized as follow: The reactive cells for Grimelius were frequently found in the gastrointestinal tract, whereas the reactive cells for Hellman-$Hellerstr{\hat{o}}m$ were found numerous in the fundus and pylorus of stomach, a few in the duodenum and lower small intestine, and very few in the rectum during both phases. No reactive cells for Masson-Fontana were found in the gastrointestinal tract during both phases. Elecron microscopically, 4 types of endocrine cells in the fundus of the stomach, 3 types in the pylorus of the stomach and duodenum, and 1 type in the lower small intestine and rectum, respectively, were identified during the hibernating phase. In the active phase, 3 types of endocrine cell in the fundus of the stomach, 2 types in the pylorus of the stomach and duodenum, and 1 type in the lower small intestine and rectum were observed, respectively. In the hibernating phase, more cytoplasmic granules and various types of endocrine cells were generally found than in the active phase.

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Association between Expression of 8-OHdG and Cigarette Smoking in Non-small Cell Lung Cancer

  • An, Ae Ri;Kim, Kyoung Min;Park, Ho Sung;Jang, Kyu Yun;Moon, Woo Sung;Kang, Myoung Jae;Lee, Yong Chul;Kim, Jong Hun;Chae, Han Jung;Chung, Myoung Ja
    • Journal of Pathology and Translational Medicine
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    • v.53 no.4
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    • pp.217-224
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    • 2019
  • Background: Exposure to cigarette smoking (CS) is a major risk factor for the development of lung cancer. CS is known to cause oxidative DNA damage and mutation of tumor-related genes, and these factors are involved in carcinogenesis. 8-Hydroxydeoxyguanosine (8-OHdG) is considered to be a reliable biomarker for oxidative DNA damage. Increased levels of 8-OHdG are associated with a number of pathological conditions, including cancer. There are no reports on the expression of 8-OHdG by immunohistochemistry in non-small cell lung cancer (NSCLC). Methods: We investigated the expression of 8-OHdG and p53 in 203 NSCLC tissues using immunohistochemistry and correlated it with clinicopathological features including smoking. Results: The expression of 8-OHdG was observed in 83.3% of NSCLC. It was significantly correlated with a low T category, negative lymph node status, never-smoker, and longer overall survival (p<.05) by univariate analysis. But multivariate analysis revealed that 8-OHdG was not an independent prognostic factor for overall survival in NSCLC patients. The aberrant expression of p53 significantly correlated with smoking, male, squamous cell carcinoma, and Ki-67 positivity (p<.05). Conclusions: The expression of 8-OHdG was associated with good prognostic factors. It was positively correlated with never-smokers in NSCLC, suggesting that oxidative damage of DNA cannot be explained by smoking alone and may depend on complex control mechanisms.

Immunohistochemical study on the gastro-entero-pancreatic(GEP) endocrine cells of the blue fox, Alopex lagopus (북극여우의 위장췌 내분비세포에 관한 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.33 no.4
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    • pp.579-589
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    • 1993
  • The regional distribution and the relative frequencies of endocrine cells were studied in nine portions of the blue fox GI tract, and the distribution pattern and cell types of the pancreativc endocrine cells were also studied in the pancreas by immunohistochemical method. Six kinds of immunoreactive cells were identified in the GI tract, and four kinds of immunoreactive cells were also identified in the pancreas. Although numerous 5-HT- and somatostatin-immunoreactive cells were seen throughout the GI tract, somatostatin- immunoreactive cells were a few in the intestine. Very numerous Gas/CCK-immunoreactive cells were restricted generally in the pyloric region and duodenum. Numerous glucagon-immunoreactive cells were found in the stomach except the pyloric region, and generally a few in the intestine. Moderate number of BPP-immunoreactive cells were found in the stomach except the pyloric region, and a few in the large intestine. Numerous porcine CG-immunoreactive cells were restricted to the cardiac and fundic region. In the pancreas, four types of pancreatic endocrine cells- somatostatin-, glucagon-, BPP- and insulin-immunoreactive- were identified in the pancreatic islet and exocrine portion. These results suggest that the regional distribution, the relative frequencies and cell types of the GEP endocrine cells in the GI tract and pancreas varies considerably among the species.

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