• Title, Summary, Keyword: eriodictyol

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Antioxidant Effects of Eriodictyol on Hydrogen Peroxide-Induced Oxidative Stress in HepG2 Cells (산화스트레스가 유도된 HepG2 세포에서 Eriodictyol의 항산화 효과)

  • Joo, Tae-Woo;Hong, Sung-Hyun;Park, Sun-Young;Kim, Gur-Yoo;Jhoo, Jin-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.4
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    • pp.510-517
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    • 2016
  • This study was conducted to investigate the antioxidant and hepatoprotective effects of eriodictyol compound against hydrogen peroxide-induced oxidative stress in HepG2 cells by measuring expression levels of antioxidant enzymes, liver function index enzyme activities, and inhibitory effects against reactive oxygen species (ROS) production. HepG2 cell viability was assessed using 3-(4,5-dimethyl thiazole-2-yl)-2,5-diphenyl tetrazolium bromide assay. In the concentration range of $10{\sim}50{\mu}g/mL$, eriodictyol displayed over 98% cell viability in HepG2 cells. The effects of increased gene expression on hydrogen peroxide-induced oxidative stress were analyzed by monitoring antioxidant enzyme (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPx) gene expression levels using real-time PCR. Eriodictyol compound significantly increased gene expression levels of SOD, CAT, and GPx in a dose-dependent manner ($10{\sim}50{\mu}g/mL$). Hepatoprotective effects against hydrogen peroxide-induced oxidative stress were analyzed by monitoring glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT) activities in HepG2 cell culture medium using a biochemistry analyzer. Eriodictyol compound significantly reduced GOT, LDH, and GGT activities in a dose-dependent manner in HepG2 cells. ROS level in HepG2 cells was analyzed by 2',7'-dichlorofluorescein fluorescence diacetate assay, and eriodictyol compound effectively reduced the intracellular ROS level in HepG2 cells. The results reveal that eriodictyol compound can be useful for development of effective antioxidant and hepatoprotective agents.

Integrated miRNA and mRNA Expression Profiling in Response to Eriodictyol in Human Endothelial Cells

  • Lee, Seung Eun;Park, Hye Rim;Yun, Hong Duck;Cho, Jeong-Je;Ahn, Hyun-Jong;Park, Cheung-Seog;Park, Yong Seek
    • BioChip Journal
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    • v.11 no.3
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    • pp.188-195
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    • 2017
  • Eriodictyol, a flavonoid commonly found in citrus fruits, shows neurotrophic, antioxidant, anti-inflammatory, and anti-cancer effects under diverse pathophysiological conditions such as vascular diseases. In this study, we evaluated whether eriodictyol modulates miRNA and mRNA expression. Using microarray analysis, we examined miRNA and mRNA expression in human endothelial cells treated with $10{\mu}M$ of eriodictyol for 24 h. Using numerous bioinformatic systems, we evaluated the signatures of the potential biological processes and signaling pathways. Our results provide insight into the underlying molecular mechanisms of action of eriodictyol and suggest that eriodictyol can be used for therapeutic intervention in vascular disease.

Binding model for eriodictyol to Jun-N terminal kinase and its anti-inflammatory signaling pathway

  • Lee, Eunjung;Jeong, Ki-Woong;Shin, Areum;Jin, Bonghwan;Jnawali, Hum Nath;Jun, Bong-Hyun;Lee, Jee-Young;Heo, Yong-Seok;Kim, Yangmee
    • BMB Reports
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    • v.46 no.12
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    • pp.594-599
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    • 2013
  • The anti-inflammatory activity of eriodictyol and its mode of action were investigated. Eriodictyol suppressed tumor necrosis factor (mTNF)-${\alpha}$, inducible nitric oxide synthase (miNOS), interleukin (mIL)-6, macrophage inflammatory protein (mMIP)-1, and mMIP-2 cytokine release in LPS-stimulated macrophages. We found that the anti-inflammatory cascade of eriodictyol is mediated through the Toll-like Receptor (TLR)4/CD14, p38 mitogen-activated protein kinases (MAPK), extracellular-signal-regulated kinase (ERK), Jun-N terminal kinase (JNK), and cyclooxygenase (COX)-2 pathway. Fluorescence quenching and saturation-transfer difference (STD) NMR experiments showed that eriodictyol exhibits good binding affinity to JNK, $8.79{\times}10^5M^{-1}$. Based on a docking study, we propose a model of eriodictyol and JNK binding, in which eriodictyol forms 3 hydrogen bonds with the side chains of Lys55, Met111, and Asp169 in JNK, and in which the hydroxyl groups of the B ring play key roles in binding interactions with JNK. Therefore, eriodictyol may be a potent anti-inflammatory inhibitor of JNK.

Studies on Biological Activity of Wood Extractives(VII) - Antimicrobial and Antioxidation Activities of Extractives from the Heartwood of Prunus sargentii - (수목추출물의 생리활성에 관한 연구(VII) - 산벚나무 심재 추출성분의 항균 및 항산화활성 -)

  • Lee, Sung-Suk;Lee, Hak-Ju;Choi, Don-Ha
    • Journal of the Korean Wood Science and Technology
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    • v.29 no.2
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    • pp.140-145
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    • 2001
  • Antimicrobial and antioxidative activities on heartwood extractives of domestic species were investigated to develop a natural fungicide or preservative. Six flavanones including pinostrobin, eriodictyol, naringenin, pinocembrin, taxifolin and verecundin were isolated from Prunus sargentii which has been selected due to its high antimicrobial and antioxidative activities among the tested species. According to the results of antifungal test, pinocembrin was evaluated as the highest antifungal compound among the test compounds, which showed 80% of hyphal growth inhibition rate. Antifungal activity of pinocembrin was similar to hinokitiol(${\beta}$-thujaplicin), strong antimicrobial compound isolated from Thujopsis dolabrata. Naringenin followed pinocembrin in its antifungal activity. However, verecundin did not show any antifungal activity. No compound was effective in antibacterial activities. As a result of the measurement of free radical scavenging activity, antioxidative activities of taxifolin and eriodictyol were 2 times that of ${\alpha}$-tocopherol, and antioxidative index of these compounds were even superior to that of ${\alpha}$-tocopherol. In this regard, it could inferred that high antifungal and antioxidative activities of extractives of P. sargentii were derived from pinocembrin, taxifolin and eriodictyol, respectively.

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Studies on the Flavonoid Constituents of Leaves of Lespedeza maximowiczii(I) (Lespedeza maximowiczii엽(葉)의 풀라보노이드 성분(成分) 연구(硏究)(I))

  • Han, Seong-Sun
    • Korean Journal of Pharmacognosy
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    • v.7 no.3
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    • pp.209-210
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    • 1976
  • To identify eriodictyol from the leaves of Lespedeza maximowiczii C.K. $S_{CHNEIDER}$, growing in Korea, the fresh leaves collected at Mt. Chil Bo, Geong Gi Province were used in the investigation. A white crystal was isolated from the water extract and it was identified as eriodictyol by its melting point, physico-chemical properties, thin layer chromatography and infrared absorption spectrum patterns.

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Flavonoid Extractives of Populus albaglandulosa (현사시나무의 후라보노이드 추출성분(抽出成分))

  • Ham, Yeon-Ho;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.23 no.2
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    • pp.94-99
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    • 1995
  • 현사시나무의 목질부와 수피부를 아세톤-물(1:1)의 혼합용액으로 추출하고 Sephadex LH-20로 충진한 칼럼을 사용하여 4개의 후라보노이드, 즉 (+)-catechin, (+)-dihydroquercetin, eriodictyol 및 (+)-dihydrokaempferol을 단리하고 $^{13}C$-NMR과 $^1H$-NMR 스펙트럼을 이용하여 그 구조를 규명하였다. 단리된 화합물의 A-환은 모두 후로로그루시놀형으로, B-환은 카테콜형 또는 페놀형으로 구성되어 있으며 (+)-dihydroquercetin, (+)-dihydrokaempferol과 eriodictyol은 포플라속의 수종에서는 처음으로 단리 되었다.

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Phytochemical Study on the Vitis thunbergii var. sinuata

  • Jon
    • Korean Journal of Plant Resources
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    • v.9 no.1
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    • pp.55-62
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    • 1996
  • The caffeic acid, 4-O-$\beta$-D-glucopyranosyl caffeic acid, 4-O-$\beta$-D-glucopyranosyl-$\rho$-coumaric acid and 7-O-$\beta$-D-glucuronide of ($\pm$)-eriodictyol have been isolated from the root of Vitis thunbergii var. sinuata. The structures of compounds were determined by chemical and spectroscopic methods.

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Isolation and Identification of Flavonoids from Ethanol Extracts of Artemisia vulgaris and Their Antioxidant Activity (쑥의 에탄올 추출물에 함유된 Flavonoid들의 분리 및 동정과 이들의 항산화 효과)

  • Lee, Sang-Jun;Chung, Ha-Yull;Lee, In-Kyoung;Yoo, Ick-Dong
    • Korean Journal of Food Science and Technology
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    • v.31 no.3
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    • pp.815-822
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    • 1999
  • Twenty one flavonoids were isolated from ethyl acetate layer of aqueaus EtOH extracts of Artemisia vulgaris and identified as tricin, jaceosidine, eupafolin, diosmetin, chrysoeriol, homoeriodictyol, isorhamnetin, apigenin, eriodictyol, luteolin, luteolin 7-glucoside, kaempferol 3-glucoside, kaempferol 7-glucoside, kaempferol 3-rhamnoside, kaempferol 3-rutinside, quercetin, quercetin 3-glucoside, quercetin 3-galactoside, quercetrin, quercetin 7-glucoside, rutin, and vietexin. The inhibitory activity for all purified flavonoids were examined against lipid peroxidation in rat liver microsome. All examined flavonoids showed considerable antioxidant activity. Among them, $IC_{50}$ value of apigenin, luteolin, isorhamnetin, quercetin, and eriodictyol were showed higher than that of vitamin E used as positive control. And methoxylated flavonoids, tricin, eupafolin, jaceosidine, diosmetin, and isorhamnetin showed considerable antioxidant activity. Each $IC_{50}$ values were shown at 0.9, 1.0, 1.4, 1.0, and $0.7\;{\mu}g/mL$, respectively.

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Studies on the Monoamine Oxidase Inhibitors of Medicinal Plants I. Isolation of MAO-B inhibitors from Chrysanthemum indicum

  • Han, Yong-Nam;Noh, Dong-Boon;Han, Dae-Suk
    • Archives of Pharmacal Research
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    • v.10 no.2
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    • pp.142-147
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    • 1987
  • Fourteen kinds of medicinal plants were screened for determining inhibitory activities on monoamine oxidase B. The extracts of Artemisia Messer-Schmidtiana (herba), Chrysanthemum indicum(flos), Ericibe obtusifolia (radix et rhizoma) and Sophora japonica (flos) strongly inhibited the enzyme. Among them, Chrysanthemi flos was chosen for elucidating its active principles, and some flavonoids were isolated and identified as acasetin (I), 5, 7-dihydroxy chromone (II), diosmetin (III), apigenin (IV), eriodictyol (V) and luteolin (VI).$IC_{50} were determined as following: 1, 2.46;II, 0.19; III, 2. 11mM, and the others showed weak inhibition.

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Antioxidative Compounds isolated from the Stem Bark of Eucalyptus globulus (유칼리나무의 수피로부터 분리한 항산화활성 물질)

  • Lee, In-Kyoung;Yun, Bong-Sik;Kim, Jong-Pyung;Chung, Sung-Hyun;Shim, Gyu-Seop;Yoo, Ick-Dong
    • Korean Journal of Pharmacognosy
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    • v.29 no.3
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    • pp.163-168
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    • 1998
  • Seven antioxidative compounds were isolated from chloroform and ethyl acetate extracts of the stem bark of Eucalyptus globulus (Myrtaceae). They were identified as rhamnazin (1), rhamnetin (2), naringenin (3), eriodictyol (4), quercetin (5), taxifolin (6) and dihydrokaempferol-3-rhamnoside (7) on the basis of various spectroscopic analyses. These compounds inhibited lipid peroxidation with $IC_{50}$ values of 0.08-30 ${\mu}g/ml$.

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