• Title, Summary, Keyword: esterase

Search Result 454, Processing Time 0.057 seconds

Studies on Esterase of Pieris rapae L. I. Changes of Esterase Activity and Zymogram Pattern During Development and Purification (배추흰나비(Pieris rapae L.)의 esterase에 관한 연구 I. 변태에 따른 esterase의 활성변화 및 zymogram pattern의 변화와 정제)

  • 박철호;김학열;여성문
    • The Korean Journal of Zoology
    • /
    • v.33 no.3
    • /
    • pp.330-336
    • /
    • 1990
  • Changes in esterase activity and zymogram pattern during development in Pieirs rapae L. were investigated and three esterases (E2, E6 and E11) from the late 5th instar larvae were purified. Esterase activity in whole body increased rapidly during 5th instar larval stages and reached a peak at the late 5th instar larval stage. The number and intensity of esterase band from whole body and midgut also showed a peak at the late 5th instar larval stage. Purification of esterase was performed using gel filtration on Sephadex G-lOO, ion-exchange chromatography on DEAE-trisacryl and preparative electrophoresis. The final purities of these enzymes were about 30 to 60-fold.

  • PDF

Evaluation of Toxicity of 23 Pesticides against Harmonia axyridis (Coleoptera: Coccinellidae) Eggs and Adults: Effect on Esterase Activity, Hatchability, and Fecundity (포식성 무당벌레(Harmonia axyridis) 난(卵)의 일부살충제와 살균제에 대한 esterase 활성 및 산란율, 부화율 조사)

  • Cho, Sae-Youll;Park, Young-Man;Park, Yong-Chul
    • The Korean Journal of Pesticide Science
    • /
    • v.11 no.2
    • /
    • pp.117-124
    • /
    • 2007
  • Esterase activity was observed after pesticides treatment in eggs of H. axyridis to select low toxicity pesticide. Egg esterases of H. axyridis were examined using an esterase substrate(${\alpha}$-naphthyl acetate). Three esterase isozymes were detected and the activities were inhibited by organophosphorus insecticide (Chlorpyrifos and Phenthoate), organochlorine insecticide(Methidation), triazole fungicide(Hexaconazole and Triflumizole), and pyrimidine fungicide(Nuarlmol). Fecundity and hatchability in adults and eggs of H. axyridis were examined on selected pesticides. Fecundity and hatchability were significantly reduced from H. axyridis adults and eggs treated with the pesticides and the fungicides showed strong inhibition of esterase isozymes activities. However, we also observed the pesticides and the fungicides showed low or non-inhibition of esterase isozymes activities affected on fecundity and hatchability in adults and eggs.

Esterase Isozyme Banding Pattern in Leaf and Stem of Legume Plants (콩과식물의 잎과 줄기의 Esterase Isozyme Banding Pattern에 관한 연구)

  • 이성규
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.11 no.4
    • /
    • pp.199-202
    • /
    • 1991
  • The esterase isozyme of several legume plants were separated and visualized by horizontal starch gel electrophoresis using enzyme-specific staining. Extracts used were prepared from fully expanded young leaf and stem of six legume species which were red clover(Trifolium Pretense L.), ladino clover(Trifolium repense L.), wild white clover(Trifolium repense L.), alfalfa(Medicage sativa L.), mimosoides(Cassia mimosoides var nomame Makino), and amoena(Vicia amoena Fisch). The number of band, Phenotype and staining intensity of esterase isozyme in leaf and stem varies depending on the plant species. However, there are little difference between leaf and stem esterase isozyme in same species except alfalfa. And in the leaf and stem of mimosoides and amoena showed not any esterase(Fig. 2). Among the examined plants, the highest staining intensity and the rapidest migrating esterase isozyme was Est 1.

  • PDF

Changes of Esterase Isozymes During the Development from Plodia interpunctella (Hiibner) (화랑곡나방의 발생에 따른 Esterase Isozymes의 Pattern변화)

  • Park, Hee-Yun;Lee, Hyung-Chul;Yoo, Chong-Myung
    • Journal of the Korean Society of Tobacco Science
    • /
    • v.20 no.1
    • /
    • pp.80-86
    • /
    • 1998
  • Changes in activity and classification of esterase isozymes during the tire cycle or Plodia inteipunctella (Hiibner) were investigated by the native polyacrylamide gel electrophoresis. The stage specificity in esterase activity and isozyme pattern was observed throughout the larvalpupal-adult transformation. The activity esterase was highest at the 3-day old adult stage, and the lowest level at the prepupal stage. A total of 12 esterase bands were identified throughout the development, and the bands showing high enzyme activity was observed in the middle part of gel. Twelve esterases on the basis of inhibition by the three types of inhibitors (organophosphates, eserine sulfate and sulfhydryl reagents) were classified into three class, namely, carboxylesterase (CE), arylesterase (ArE) and cholinesterase (ChE), and these classes contained 7, 3 and 2 isozymes, respectively.

  • PDF

Purification and Characterization of Acetyl Xylan Esterase II from Escherichia coli Cells Harboring Recombinant Plasmid pKMG7 (재조합 균주 Escherichia coli가 생산하는 Bacillus stearothermophilus Acetyl Xylan Esterase II의 정제 및 특성)

  • 김희선;서정한;최용진
    • Microbiology and Biotechnology Letters
    • /
    • v.23 no.4
    • /
    • pp.454-460
    • /
    • 1995
  • Acetylxylan esterase II was produced by Escherichia coli HB101 harboring the recombinant plasmid pKMG7 which contained the estII gene of Bacillus stearothermophilus. Optimal medium for the production of the acetylxylan esterase by E. coli HB101/pKMG7 was determined to contain 0.5% galactose, 1% yeast extract and 1% NaCl. The enzyme produced was purified to homogeneity using a combination of 20-50% ammonium sulfate precipitation, DEAE-Sepharose CL-6B chromatography and Sephacryl S-200 gel filtration. The temperature and pH optimum of the esterase were 45$\circ$C and pH 6, respectively. The essential amino acids for the esterase activity were found to be methionine, serine, and cysteine. Molecular weight of the esterase was determined to be 28 kDa by SDS-polyacrylamide gel electrophoresis, and 120 kDa by gel filtration. This suggests that the functional enzyme is a homomeric tetramer. The esterase had an isoelectric point of pH 3.4. The N-terminal amino acid sequence of the enzyme was Ala-Leu-Phe-Glu-Ser-Arg-Phe-Phe-Ser-Glu-Val-Leu-Gly-Leu.

  • PDF

Studies on the Rearing with Artificial Diet in the Silkworm, Bombyx mori L. -Electrophoretic Sepatarion of Esterase or Phosphatase along the Growth of Larvae- (가잠의 인공사료육에 관한 연구 -유충발육에 따른 Esterase 및 Phosphatase의 전기영동상-)

  • 김주읍
    • Journal of Sericultural and Entomological Science
    • /
    • v.21 no.1
    • /
    • pp.21-28
    • /
    • 1979
  • The Electrophoretic separation in agarose gel on the esterase and acid phosphatase of blood, midgut and silk gland was carried out with 2 original variginal varieties and 7 F$_1$ hybrids. 1. The midgut of larvae fed on mulberry leaves showed one or two more esterase bands than that of larvae fed on artificial diet. 2. The midgut of C 15 larvae being excellently respondent to artificial diet showed one or two more esterase bands than that of larvae being bad respondent to artificial diet. 3. Electrophoretic separation of esterase bands appeared to be greatly different among newly hatched larvae, 1st and 2nd install larvae of F$_1$hybrids. However the difference among the silkworm varieties was not recognized. 4. According to the change in rearing temperature, the number of the active band of midgut esterase was varied. At the temperature of 28$^{\circ}C$ 5 active esterase bands were found. At temperature of 35$^{\circ}C$ 4 bands were noted at 3rd install and 6 or 7 bands at 4th instar. 5. No similar esterase bands conld be found among midgut, blood and silkgland. There are five esterase bands in the midgut, one in blood and three in silkgland. 6. There was rather small difference in acid phosphatase types of midgut and blood according to varieties and rearing temperature. No active band was shown in silkgland. In midgut, there was one acid phosphatase band at 3rd install, two at 4th instar and three at 5th instar. In blood, One active band at 3rd or 4th instar and three bands at 5th inatar were detected.

  • PDF

Effect of Esterases from Rice Wine Yeast on the Ethyl Caproate Production during Rice Wine Brewing. (청주 제조 중 Ethyl Caproate 생성에 미치는 청주효모 Esterases의 영향)

  • 이종훈
    • Microbiology and Biotechnology Letters
    • /
    • v.26 no.1
    • /
    • pp.50-54
    • /
    • 1998
  • Ethyl caproate is one of the important flavor compounds produced during the brewing of rice wine. The rice wine yeast and koji were reported to produce the esterases which synthesize and also hydrolyze ethyl caproate. From the results of monitoring the esterase activities of rice wine yeast and koji, their roles for producing ethyl caproate during brewing were postulated. In case of rice wine yeast, the production of esterase synthesizing ethyl caproate was influenced by the substrate, caproate but that of esterase hydrolyzing ethyl caproate was promoted by ethyl caproate but inhibited by caproate. The production of esterases of koji were not influenced by the substrates for ethyl caproate production but influenced by the growth of koji. The maximum concentration of ethyl caproate produced by rice wine yeast was 0.4 ppm in this research but the production of ethyl caproate by koji was not detected under our experimental conditions. Considering the results of this research, ethyl caproate is not produced by the esterases of koji during brewing but produced by the esterases of rice wine yeast. The growth of rice wine yeast represses that of koji because of the high concentration of ethanol produced by rice wine yeast. The esterases of rice wine yeast may decide the production of ethyl caproate during brewing.

  • PDF

Studies on the Insecticide Resistance of the German Cockroach(Blattella germanica L.). III. Comparison of Esterase Activity (바퀴(Blattella germanica L.)의 살충제 저항성에 관한 연구. 3. Esterase활성비교)

  • 방종렬;김정화;이형래
    • Korean journal of applied entomology
    • /
    • v.32 no.3
    • /
    • pp.265-270
    • /
    • 1993
  • The German cockroach(Blattelia germanica) population~ were successIVely selected with ch\orpyrifos and permethrin during the six generations. The resulting resistant $R_{chtorpenfos}$(Rc) and $R_{permethnn}$(Rp) stra.ins were studied to investigate the esterase activity by spectrophotometer, filter parper test, and electrophoresis. Esterase-$\alpha$ activities by filter paper test showed 2.65 and LBZ times higher in the Rc and Rp strains than the susceptible strain, respectively. ln the spectrophoLometer method, the esterase activit18s to $\alpha$-and $\beta$-naphthyl acetate were increased 2.34 and 5.28 times in the Rc than susceptible strain, and 1.48 and 2.92 times in the Rp Limn susceptible stram, respectlvely. Zymogram patterns of eslerase isozyme by agarose gel electrophoresis showed totally five bands. The Rc and Rp strains showed two additive bands as, Est-2 and Est-3, which were not shown in the susceptible strain. but the Rp strain dId not show Est-5 bands which was COlumon in the Rc and susceptible strams.

  • PDF

Esterase Production and Culture Characteristics of Bacteria Isolated from Acid Hydrolysed Soybean Protein (산분해 대두 단백질로부터 분리된 Esterase 생성균의 생육 및 효소생성 특성)

  • Oh, Nam-Soon
    • Applied Biological Chemistry
    • /
    • v.40 no.6
    • /
    • pp.484-489
    • /
    • 1997
  • The characteristics of growth and esterase activity of bacterial strains isolated from acid hydrolysed soybean protein were examined. All the isolated strains having decomposition activity of p-hydroxybenzoic acid butyl ester and esterase producing activity were identified as Bacillus sp. by morphological and biochemical methods. The specific growth rates, esterase activities and p-hydroxybenzoic acid butyl ester decomposition activities of isolated strains were $0.844{\sim}1.213\;h^{-1}$, $21{\sim}222\;mU/ml$ and $5.4{\sim}8.1\;mU/ml$, respectively. In the fermentation of Bacillus sp. KB8 strain which had the highest esterase producing activity, growth, extracellular excretion and intracellular synthesis of esterase were inhibited by adding NaCl in the culture broth. Esterase producing activity gradually increased after late exponential growth phase, until maximum value of 420 mU/ml reached after 64 hours culture period. Esterase of Bacillus sp. KB8 strain was stable up to $50^{\circ}C$ for 30 minutes, but was inactivated by heating for 30 minutes at $70^{\circ}C$. The enzyme activity exponentially decreased during the incubation time at the temperatures of $60^{\circ}C$ and $65^{\circ}C$.

  • PDF

Optimization of Induction Conditions for Bacillus-derived Esterase Production by High-cell Density Fermentation of Recombinant Escherichia coli (재조합 대장균의 고농도 배양과 유도조건 최적화를 통한 Bacillus 유래 esterase의 생산)

  • Kang, Seung-Hoon;Min, Byung-Hyuk;Choi, Hong-Yeol;Kim, Dong-Il
    • Microbiology and Biotechnology Letters
    • /
    • v.45 no.2
    • /
    • pp.149-154
    • /
    • 2017
  • To increase the efficiency of esterase production by Bacillus, high cell-density culture of recombinant Escherichia coli through fed batch fermentation was tested. Cells were cultured to $OD_{600}$ of 76 (35.8 g/l DCW) with dissolved oxygen level controlled to least above 30% air saturation by supplying pure oxygen. Cells were cultured to an $OD_{600}$ of 90 (42.4 g/l DCW) with glucose feeding controlled to at least 1 g/l. However, the cells reached stationary phase at the late stage of culture, despite glucose being supplied. Cells were cultured to an $OD_{600}$ of 185 (87.3 g/l DCW) by supplying additional medium with fortified yeast extract. To increase the productivity of the recombinant protein, cell growth and esterase productivity based on induction time were evaluated. Late exponential phase induction for esterase production in fed batch fermentation resulted in maximum optical density $OD_{600}$ of 190 (89 g/l DCW) and maximum esterase activity of 1745 U/l, corresponding to a 5.8-fold enhancement in esterase production, compared to the early exponential phase induction. In this study, we established fermentation methods for achieving maximum production of Bacillus-derived esterase by optimizing IPTG induction time in high-cell density culture by supplying pure oxygen and a nitrogen source.