• Title/Summary/Keyword: exudate

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Changes of Characteristics in Salted Baechu(Chinese Cabbage) and Its Exudate during Long Term Storage (장기저장중 절임 배추와 그 삼출액의 특성변화)

  • Han, Eung-Su;Seok, Mun-Sik;Park, Ji-Hyeon
    • Korean Journal of Food Preservation
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    • v.5 no.2
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    • pp.162-166
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    • 1998
  • Chemical, physical md microbial analysis of salted baechu(Chinese cabbage) and its exudate were conducted during storage in large plastic bags(LDPE, HDPE, PVC-box) at 0$^{\circ}C$. Salinity was slightly lowered for 2 weeks storage and maintained that level thereafter in salted haechu, but in its exudate increased rapidly for 2 weeks storage and decreased thereafter. In all treatment pH decreased to 5.0 in salted baechu and to 4.5 in its exudate for 6 weeks storage. Reducing sugar content of salted baechu was 1.5∼2.0 fold higher than that of its exudate, and decresed slowly in both. Total viable cells increased rapidly for 2 weeks but maintained that level thereafter and lactic acid bacteria increased continuously until 6 weeks. Lightness of exudate decreased rapidly in PVC-box, but decreased slowly in LDPE. Compression force of salted baechu increased continuously untill 8 weeks except for PVC-box.

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Analysis of Activative Inhibitors of Chrysanthemum from Root Exudate of Allium fistulosum (대파 뿌리 분비물내의 국화 생장 억제 활성물질 분석)

  • 최상태;안형근;박인환
    • Asian Journal of Turfgrass Science
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    • v.13 no.3
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    • pp.171-176
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    • 1999
  • Chrysanthemum showed worse grow of wilt to death during summer at the field which is Allium fistulosum (welsh onion) plants had been cultivated. This study was carried out to analysis of activative inhibitors of chrysanthemum from root exudate of Allium fistulosum. Bioassay experiments with welsh onion root exudate were conducted and the biologically active compounds were determined. The results were obtained as follows. The root exudate of welsh onion inhibited root and hypocotyl growth of chrysanthemum and lettuce at low concentration(10ppm). The inhibitory effects was higher in closed bottom box but with drain hole than in open bottom box plot. The inhibitory substance contained in root exudate was analysed as vanillic acid. This phenolic acid was also detected in stem-leaf and root of welsh onion.

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The Analysis of Canavanine Content in Leaves, Roots, and Xylem Exudate of Canavalia lineata (해녀콩(Canavalia lineata)의 잎, 뿌리 및 도관액에서 Canavanine의 함량분석)

  • 박경순
    • Journal of Plant Biology
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    • v.33 no.2
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    • pp.119-126
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    • 1990
  • The content of canavanine was measured and analyzed in leaves, roots and xylem exudate of Canavalia lneata. In non-nodulated plants, the cotyledons were removed after a week of sowing and the plants were grown for 3 weeks. The quantity of canavanine measured by canavanine specific-PCAF colorimetric assay was 9-10 $\mu$mol/g fresh wt. in leaves, 5-6 $\mu$mol/g fresh wt. in roots, and 0.3-0.5 $\mu$mol/ml in xylem exudate. When free amino acids of leaves, roots, and xylem exudate were analysed by HPLC, the relative proportion of asparagine plus glycine was the highest and canavanine was high secondarily. And the relative proportion of canavanine among total free amino acids was 30-35% in leaves and roots, and 12-13% in xylem exudate. In non-nodulated plants grown for 8 weeks, the canavanine content of each part was similar to that of 3-week-old plants. By the formation of nodules, the canavanine content of leaves, roots, xylem exudate, and nodules decreased apparently. In xylem exduate, the nitrogenous compounds were also analyzed. The relative contents of NO3-, free amino acids, and ureides(allantoin and allantoic acid) were 60-80%, 20-30%, and 5%, respectively. From these results, it can be assumed that canavanine is synthesized in the root of plant and nodulation affects the canavanine content. It is obvious that canavanine is considered one of the reduced-N forms transported via xylem.

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Potentiation of Inducible Nitric Oxide Expression by Indomethacin in Carageenin-treated Rat Paw Inflammation (Carrageenin으로 흰쥐 발 염증으로 Indomethacin에 의한 유도성 nitric oxide synthase의 발현증가)

  • 원혜영;강건욱;김영미;김낙두
    • YAKHAK HOEJI
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    • v.43 no.2
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    • pp.214-220
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    • 1999
  • Present study was aimed to examine whether indomethacin affected the production of NO in the rat paw exudate by carrageenin. Paw edema and nitrite/nitrate levels in the paw exudate were maximal after 4 h and remained elevated up to 10 h, whereas indomethacin (10 mg/kg, po) significantly inhibited the carrageenin-induced paw edema and levels of nitrate in the paw exudate. However, paw edema and nitrite/nitrite levels were increased thereafter for 10 h. Indomethacin also enhanced the expression of iNOS mRNA and protein 4 h after carrageenin infection. Indomethacin inhibited the level of $PGE_2$ in the paw exudate in a time-dependent manner. These results suggest the possibility that indomethacin may potentiate expression of iNOS and subsequently increase nitrite/nitrate level in the late phase of carrageenin-induced rat paw inflammation possibly by suppressing cycloxygenase activity.

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A Case of Atopic Dermatitis Patient with Exudate Using Korean Medicine Treatment and Western Treatment (한양방 치료를 병행한 삼출액을 주소로 하는 아토피피부염 치험 1례)

  • Kim, Hye-Hwa;Park, Soo-Yeon;Kim, Jong-Han;Jung, Min-Yeong;Choi, Jung-Hwa
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.28 no.4
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    • pp.156-164
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    • 2015
  • Objectives : This study is clinical report of one atopic dermatitis patient with exudate.Methods : We treated a 16-year old woman patient with acupuncture, pharmacopuncture, herbal medicine, herbal bath, aroma cream therapy and western medicine. The improvement of the patient was evaluated by SCORAD INDEX and photography.Results : The SCORAD Index score of admission day at hospital was 73.5, and the SCORAD INDEX score of discharge day was 11.4. Amount of exudate and the score of oozing were decreased.Consults : The complex treatments of korean medicine and western medicine was effective to improve of atopic dermatitis patient with exudate. And the influence of weather condition to prognosis of atopic dermatitis with exudate was observated. It is necessary to have more observations and case reports.

Diagnostic Significance of Cholesterol in Pleural Effusion (흉막액내 Cholesterol치 측정의 진단적 의의)

  • Yeo, Dong-Seung;Lee, Dong-Il;Lee, Soo-Keol;Moon, Chang-Hyung;Park, Soon-Kew;Shin, Young-Kee
    • Tuberculosis and Respiratory Diseases
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    • v.39 no.3
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    • pp.248-254
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    • 1992
  • Background: Pleural effusion is one of the most common clinical problems in pulmonology because of high prevalence of pulmonary tuberculosis and bronchogenic carcinoma in Korea. The differential diagnosis between pleural transudate and exudate is very important, but it is very difficult in some cases. Methods: In order to assess the clinical usefulness of cholesterol levels for the differential diagnosis of pleural transudate and exudate, we measured pleural fluid cholesterol levels by enzymatic method in 45 patients who were admitted due to pleural effusion. Results: The mean cholesterol level of transudate was $33.1{\pm}12.9\;mg%$, tuberculous exudate was $97.3{\pm}28.2\;mg%$ and malignant exudate was $97.3{\pm}28.2mg%$. When the cut-off value of pleural cholesterol level was 60 mg%, one case (6.7%) of tuberculous exudate and two cases (13.3%) of malignant exudate were incorrectly classified, but all cases of transudate were classified correctly. When the cut-off value of pleural/serum cholesterol ratio was 0.3, one case (6.7%) of transudate and two cases (13.3%) of malignant exudate were incorrectly classified, but all cases of tuberculous exudate were classified correctly. When the cut-off value of pleural cholesterol level to differentiate pleural transudate from exudate was 60 mg%, sensitivity was 90% and specificity was 100%. When the cut-off value of pleural/serum cholesterol level to differentiate pleural transudate form exuidate was 0.3, sensitivity was 93% and specifiity was 93%. Conclusions: From the above results, it can be concluded that measurement of pleural fluid cholesterol levels is useful for the differential diagnosis between pleural transudate and exudate.

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Passive Transfer of Immunity against Clonoychis sinensis by Peritoneal Exudate Cells in Mice (복강삼출세포를 이입받은 마우스에서의 간흡충에 대한 면증응답)

  • Gwon, Tae-Chan;Gang, Jin-Mu;Choe, Dong-Ik
    • The Korean Journal of Parasitology
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    • v.25 no.1
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    • pp.45-50
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    • 1987
  • This study was undertaken to evaluate the role of peritoneal exudate cells in the transfer of immunity against the liver cuke, Clonorchis sinensis in the inbred BALB/c mice. Ten donor mice were divided into 2 groups. One group consisted of 5 mice was infected orally with 20 metacercariae of C. siitensis, and the other group was injected intraperitoneally with 20 excysted larvae. Thirty days after immunization, the peritoneal exudate cells tore obtained from the donor mice. Twenty recipient mice were divided into 4 equal groups for the purpose of primary immunization. The mice of Group I were injected intraperitoneally with $2{\times}10^6$ peritoneal exudate cells of the donor mice infected orally, those of Group II were injected intraperitoneally with $2{\times}10^6$ peritoneal exudate cells of the donor mice injected intraperitoneally. Those of Group III were injected orally with 20 metacercariae of C. sinensis. The group IV mice served as controls. Four days after the primary iMmunization all recipient mice were challenged orally with 20 metacercariae of C. sinensis, and then killed 30 days after the challenging infection. When the peritoneal exudate cells were injected into the recipient mice, pronounced reduction in eggs per gram of the feces was found in the mice o( Group I and Group II, but no reduction in those of Group III. In the worm burdens of C. sinensis, the number of flukes found in the mice of Group II was only significantly less than those in the control group (IV). In addition the number of plaque forming cells per spleen in the mice of Group II was found larger than those in Group I. It is likely that donor peritoneal exudate cells transferred to the recipients might result in the production of relative immunity.

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Diagnostic Significance of Total Lactate Dehydrogenase(LD) and LD Isoenzyme Measurement in the Body Fluids (체강액에서 총 Lactate dehydrogenase 및 Lactate dehydrogenase 동위효소 측정의 진단학적 의의)

  • Jeon, Chang-Ho;Bae, Eun-Kyung;Hong, Seok-Il;Kim, Chung-Sook;Lee, Young-Hyun
    • Yeungnam University Journal of Medicine
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    • v.3 no.1
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    • pp.193-199
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    • 1986
  • Body fluid Lactate dehydrogenase and its isoenzyme measurement was performed in 132 patients: 8 cases with peritonitis, 21 cases with malignant ascites, 43 cases with liver cirrhosis, 48 cases with tuberculous pleuritis, 12 cases with malignant pleural effusion respectively. Body fluid protein and glucose contents, red blood cell counts, white blood cell counts, cytologic examination were also performed as a comparative study. The results were as follows: 1. Measurement of total LD and protein amount could differentiate between transudate and exudate in the ascitic fluids. 2. In the malignant exudate of ascites and pleural fluid, the activity of LD2 isoenzyme was statistically increased compared with that of inflammatory exudate and the activity of LD4 isoenzyme was also increased compared with that of serum(P<0.05). 3. The inflammatory exudate of pleural fluid and ascites demonstrated the increase of LD5 isoenzyme activity stastistically compared with that of serum and malignant exudate(P<0.05). 4. A difference of total LD activity between malignant ascites and inflammatory ascites was significant statistically, while this was not observed in the pleural exudate. 5. Total LD and LD5 isoenzyme activity didn't correlated with the number of white blood cells in the exudate.

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The Effect of Root Exudate and Chemotaxis on Host Recognition in Soybean-Bradyrhizobium Symbiosis (대두(大豆)-근류균(根瘤菌) 공생(共生)에서 뿌리분비물(分泌物)과 화학주성(化學走性)이 숙주인식(宿主認識)에 미치는 영향(影響))

  • Kang, Sang Jai;Park, Woo Churl
    • Current Research on Agriculture and Life Sciences
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    • v.11
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    • pp.121-132
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    • 1993
  • This study was carried out to research the effect of the chemotaxis of Bradyrhizobium japonicum KCTC 2422 and its mutant toward soybean root exudate and to elucidate the effect of the lectin of host specificity (Host Recognition) in soybean-Bradyrhizobium symbiosis. The results obtained were as follows: The homogeneities of the purified lectins from soybean and pea seed was ascertained chromatographically and electrophoretically. Gel electrophoresis of soybean lectin in the presence of sodium dodecyl sulfate appeared a single protein band, whereas pea lectin appeared two protein bands. Soybean lectin from 2 cultivars formed immunoprecipitin arcs at same position with anti-soybean lectin rabbit IgG, but pea lectin did not form immunoprecipitin lines with anti-soybean lectin rabbit IgG. Chemotactic responses of KCTC 2422, LPN-100 and LCR-101 toward proline in capillary assays were 3.1, 1.3 and 1.0-fold above background, respectively. The chemotactic responses of KCTC 2422, LPN-100, and LCR-101 toward Paldal crude root exudate in capillary assays were 3.5, 1.4 and 1.4-fold above background, respectively. The present work shows that B. japonicum and its mutants are capable of very different responses toward root exudate fraction. The chemotactic responses of KCTC 2422 was most with neutral fraction, least with anionic fraction and intermediate with cationic fraction. The nitrogenase activity of soybean nodule was shown in 15days after inoculation with LCR-101. However, we couldn't find out the nodules when soybean was inoculated with LPN-100. From these result we can suppose that the chemotaxis of Bradyrhizobium plays inportant the role of forming the nodule (host recognition) in the soybean-B. japonicum symbiosis.

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Effect of Chemotaxis on Nodulation in Bradyrhizobium-Soybean Symbiosis (근류균의 화학주성이 근류형성에 미치는 영향)

  • Kang, Sang-Jai;Park, Woo-Churl
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.2
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    • pp.136-146
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    • 1994
  • To research the effect of chemotaxis of Rhizobia toward the root exudate on nitrogen fixing ability in soybean Rhizobia symbiosis system. Root exudate from seedlings of Glycine max. L was collected aseptic conditions. B. japonicum KCTC 2422 induced the formation of symbiotic nitrogen fixing nodules on the root of soybean plant and possessed motility and chemotaxis toward the 2mM proline. LPN-100 mutant was $Nod^-$, $Che^+$, and LPN-101 was $Che^-$, $Nod^+$ strains. Physiological properties of mutants were similar to parent strain. The crude root exudate was tested for its chemotactic ability using the capillary tube method. Chemotactic responses of RCR 3407 toward crude root exudate were 2.2, 2.6, 2.9, those of KCTC 2422 were 2.3, 2.9, 3.0, respectively. The crude root exudate was fractionated into neutral, cationic and anionic fractions. Chemotactic responses of KCTC 2422 was least with anionic fraction, most with neutral and intermediate with cationic fraction. B. japonicum KCTC 2422 was attracted by carbohydrates, amino acids and carboxylic acid. Carbohydrates and amino acids were good chemoattractants and carboxylic acids were intermediate chemoattractants. The peak concentration was $10^{-3}M$ for ribose, glucose, glutamine, aspartic acid and carboxylic acids, with exception of xylose, arabinose, tryptophan, which elicited maximum responses at $10^{-4}M$. The formation of nodules and nitrogenase activity of soybean inoculated with KCTC 2422 was determined in 7days after inoculation, and those of LPN-101 was detected in 15days after inoculation, but LPN-100 didn't form of nodules in soybean plants.

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