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Examination of Berberine Dye using GC-MS after Selective Degradation Treatments (GC-MS를 이용한 Berberine 염료의 퇴화 거동 연구)

  • Ahn, Cheun-Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.33 no.12
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    • pp.2002-2010
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    • 2009
  • The degradation behavior of berberine is examined using GC-MS to select the fingerprint products that can be used to identify berberine dye in badly faded archaeological textiles. A total of $100^{\circ}C$ thermal and $H_2O_2/UV/O_2$ degradation systems were used to degrade berberine chloride 0.1% solution up to 408 hours. The samples were analyzed using the GC-MS. Dihydroberberine, 2-pteridinamine, 6,7-dimethyl-N-[(trimethylsilyl) oxy]-, and 8-methoxy-11-[3-methylbutyl]-11H-indolo[3,2-c]-quinoline, 5-oxide were detected as the major products of thermal degradation and identified as the fingerprint products for berberine dye at the early stage of degradation. Isobenzofuran-1,3-dione,4,5-dimethoxy-, 9H-fluorene,3,6-bis(2-hydroxyethyl)-,1,3-dioxolo[4,5-g]isoquinolin-5(6H)-one,7,8-dihydro-, and 3-tert-butyl-4-hydroxyanisole were detected as the major products generated by the $H_2O_2/UV/O_2$ degradation and identified as the fingerprint products for berberine dye under severe degradation conditions.

Separation of Chromophoric Substance from Amur Cork Tree Using GC-MS (GC-MS를 이용한 황벽의 색소 성분 분리 거동)

  • Ahn, Cheun-Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.33 no.6
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    • pp.980-989
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    • 2009
  • Amur cork tree was extracted in methanol with the purpose of investigating the most effective extraction procedure for detecting the chromophore using the GC-MS analysis. Different procedures of waterbath and hotplate extractions were carried out and five different GC-MS instrument parameters including the operating temperatures in the GC capillary column and the MSD scan range were tested for their efficiencies. Berberine was determined by the detection of dihydroberberine at 15.0 min r.t. Hotplate was a better device for extracting amur cork tree than waterbath shaker either with or without presoaking in the room temperature. Water was not an adequate extraction medium for the berberine detection. The most effective GC-MS parameter was Method 4; the initial temperature at $50^{\circ}C$ followed by the temperature increase of $23^{\circ}C$/min until $210^{\circ}C$, then increase of $30^{\circ}C$/min until the final temperature reach at $305^{\circ}C$, then hold for 14 minutes to maintain the total run time 24.12 minutes. The MSD scan range for Method 4 was $35\sim400$m/z.

The potentiality of color preference analysis by EEG (뇌파분석 통한 색상의 선호도 분석 가능성)

  • Kim, Min-Kyung;Ryu, Hee-Wook
    • Science of Emotion and Sensibility
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    • v.14 no.2
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    • pp.311-320
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    • 2011
  • To quantitatively analyze the effects of color stimulation which is one of the major affecting factors on human emotion, we studied the relationship between color preference and the Electroencephalography (EEG) to 3 color stimuli; bright yellow red (BYR), deep green yellow (DGY), and vivid blue (VB). Physiological signal measured by EEG on the color stimulation was closely related with their well-known colorful images. The brain become more activated with decreasing the color temperature (BYR${\geq}$DGY>VB), and the right brain is more sensitive than the left. On the whole, the EEG values of the frequency bands are in order to beta ${\geq}$ theta and alpha > gamma. As decreasing the color temperature, beta wave increased (BYR${\geq}$DGY>VB), and alpha, beta and gamma waves increased with increasing the color temperature (BYR${\geq}$DGY>VB). The relationship between the color preference and EEG values showed EEG gets more activated at some frequency bands when the color preference becomes higher. In conclusion, the specific frequency band could be activating by a color stimuli which had showed higher the preference. It means that these color stimuli can apply for various industries such as beauty industry, interior design, fashion design, color therapy, and etc.

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Separation of Chromophoric Substance from Sappanwood under Different Extraction Conditions (염료 추출조건에 따른 소목의 색소성분 분리 거동)

  • Ahn, Cheun-Soon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.31 no.12
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    • pp.1653-1661
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    • 2007
  • The research aimed to establish the standard extraction procedure for examining brazilin, the major chromophoric substance of Sappanwood, using GC-MS with the ultimate goal of identifying the sappanwood dye in severely faded archaeological textiles. The amount of brazilin represented by the GC abundance was the largest when acetone was used as the extraction medium, followed by methanol. Shaking plate operated at room temperature was more effective than the waterbath shaker which was operated at $30^{\circ}C$. In both cases, the extraction method which incorporated one hour pre-soaking before the 12 hours of actual extraction resulted in a larger amount of brazilin detection than the extraction procedure without the one hour pre-soaking. In case of water extraction, pH 5 resulted in the most effective pH level for the extraction of brazilin, The best GC-MS parameter for detecting brazilin was to set the column temperature initially at $50^{\circ}C$. gradually increase to $210^{\circ}C$ at a $23^{\circ}C/min$ rate, finally increase to $305^{\circ}C$ at $30^{\circ}C/min$ rate, and hold for 14 minutes, and the MSD scan range at $75{\sim}400m/z$.

Comparative Analysis of Korean and American Body Sizes & Shapes using 3D Scanned Anthropometric Data (한국과 미국 성인의 3차원 인체 치수 비교)

  • Yi, Kyong-Hwa;Istook, Cynthia;Kang, Yeo-Sun;Choi, Hei-Sun
    • Journal of the Korean Society of Clothing and Textiles
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    • v.31 no.6
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    • pp.892-901
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    • 2007
  • This research is the initial step for establishing a convertible body sizing table applicable to the Korean and American female apparel industry. All 3D female measurement data of the two countries were obtained from Size Korea Project and SizeUSA Project. The sample subjects used in this study were 1,988 Korean and 6,306 American females. Thirty-four(34) variables were chosen as the principal measurements in making garments. The conclusion of this research was as follow: First, it was determined that American women were larger and longer than Korean women in all measurement except shoulder slope measurement. Second, according to the differences analysis of the each age group in Korean Females, all measurements except hip girth had significant differences among the age groups. In case of American females, all measurements except arm length(shoulder to wrist) had significant differences among the age groups. Third, in the comparison of differences between the age groups of the two countries' women, some dimensions varied significantly with age, while others did not show any statistical significance among the age groups. Fourth, according to the t-tests of same age groups between Korean & American female measurements, American female measurements were larger and longer than Korean in all measurements except crotch length total, shoulder slope, hip girth-bust girth and hip girth-waist girth.

Analysis of Degradation Products in Madder Dyed Fabrics in Selective Degradation Conditions (퇴화조건에 따른 꼭두서니 염색물의 퇴화물 연구)

  • Ahn, Cheun-Soon;Obendorf, S.-Kay
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.12
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    • pp.1608-1618
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    • 2005
  • The purpose of this investigation was to investigate the degradation products of the dye component extracted from madder dyed fabrics using the GC-MS analysis and to evaluate the change of color due to degradation treatment. Four different degradation protocols were used in this study,; refrigeration at $7^{\circ}C$ (LT), room temperature (RT), oven treatment at $100^{\circ}C$ (OV), and $H_2O_2/UV(PER)$ method. Degradation times for each thermal system were 6 hour, 24 hour, 48 hour, 1 week, 2 week, 4 week. Alizarin was detected from the control and degraded samples of both alizarin dyed and madder dyed fabrics. Benzoic acid, 2, 4-di-tert-butylphenol, phthalic anhydride were detected as the degradation products for both alizarin dyed and madder dyed fabrics. The result suggest that these products can be used as the fingerprints of GC-MS analysis for the identification of madder dye in archaeological textiles. Both alizarin dyed and madder dyed samples became less red and less yellow after degradation. In the PER degradation system madder dyed sample showed the greatest color difference even after 1 week of degradation treatment. Further research is necessary for investigating the color change in the exhumed textiles, which is caused by the dual action of dye fading and the staining of organic matters in the soil.

Glutamine Deprivation Inhibits Invasion of Human Prostate Carcinoma LnCap Cells through Inactivation of Matrix Metalloproteinases and Modulation of Tight Junctions (글루타민 결핍에 따른 Tight Junction 및 MMPs 활성 조절을 통한 전립선 암세포의 침윤 억제 현상)

  • Shin, Dong Yeok;Choi, Yung Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.8
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    • pp.1167-1174
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    • 2013
  • Cancer cells exhibit increased demand for glutamine-derived carbons to support anabolic processes. Indeed, the spectrum of glutamine-dependent tumors and the mechanisms through which glutamine supports cancer metabolism remain areas of active investigation. In the present study, we investigated the effects of glutamine deprivation on the correlation between tightening of tight junctions (TJs) and anti-invasive activity in human prostate carcinoma LnCap cells. Glutamine deprivation markedly inhibited cell motility and invasiveness in a time-dependent manner. The anti-invasive activity of glutamine deprivation was associated with an increased tightness of the TJ, which was demonstrated by an increase in transepithelial electrical resistance (TER). The activities of matrix metalloproteinase (MMP)-2 and MMP-9 were inhibited in a time-dependent fashion by glutamine deprivation, which was correlated with a decrease in expression of their mRNA and proteins and up-regulation of tissue inhibitors of metalloproteinases (TIMPs) expression. Furthermore, glutamine deprivation repressed the levels of the claudin family members, which are major components of TJs that play a key role in the control and selectivity of paracellular transport. Moreover, the levels of E-cadherin, a type I transmembrane glycoprotein, and snail, an epithelial to mesenchymal transition regulator and zinc finger transcription factor, were markedly modulated by glutamine deprivation. Taken together, these findings suggest that TJs and MMPs are critical targets of glutamine deprivation-induced anti-invasion in human prostate carcinoma LnCap cells.

Analysis of Tack Properties of Aramid/Phenolic Prepreg (아라미드섬유/페놀수지 프리프레그의 Tack성 분석)

  • Hong, Tae Min;Lee, Ji Eun;Hong, Young Ki;Lee, Jung Soon;Cho, Dae Hyun;Lee, Seung Goo
    • Journal of Adhesion and Interface
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    • v.14 no.3
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    • pp.117-120
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    • 2013
  • The prepreg material is a sheet of the reinforcement pre-impregnated with a resin. In this study, two types of prepreg were prepared with a general phenolic resin and the polyvinyl butyral (PVB) modified phenolic resin, respectively, with resin content of 40 wt%. After resin impregnation, the prepregs were heat treated in an oven to make them the B-stage. Surface morphology of the prepreg was observed by using a scanning electron microscope (SEM). Tack property of the prepreg is one of the major properties that govern the ability of prepreg to be laid up. In this study, the tack of prepreg was measured under various test parameters by a probe tact test. Test parameters were contact time, contact force and debonding rate. Most of the tack properties of the prepreg increased with the test parameters. Then tack properties exhibited a linear behavior with test parameters before a saturation point. Also, the tack of prepreg was investigated in relation with the fibrillation phenomena involved in the prepreg surface with the debonding rate.

A Study on the Excavated Clothes of Lady Shim Cheongsong in Jecheon (제천출토 청송심씨(1753~1810) 출토유물)

  • Chang, In-Woo;Park, Bong-Soon
    • Journal of the Korean Society of Costume
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    • v.64 no.2
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    • pp.150-162
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    • 2014
  • This study examined the excavated clothes of Lady Sim Cheongsong(1753~1810) in Jecheon which were excavated in 2012. Lady Sim died at the age of 57 and the excavated relics belonged to the 18th and 19th centuries. The excavated relics consisted of 9 pieces of three-kind-clothes. They showed various qualities such as Plain-Silk, Plaited-Silk, Patterned Twill, Plain Twill, Plain Satin, and Satin Damask. Through comparing them with the other excavated clothes of the 18th and 19th centuries, we can comprehend the periodical changes of the excavated Jegori and Yeomo (the hat for a dead woman). The excavated Jegori shows the difference of length and form from the other Jegori of the 18th century. The total length of the excavated Jegori ranged from 24 cm to 25 cm, which is 10 cm shorter than that of the other Jegori of the 18th century. The excavated hat for a dead woman shows the changes of the form and needlework. The form of a rectangular cover was changed into that of a round shape. In regards to the sewing composition, the way of inserting the cover into Mosin(the body of the hat for a dead woman) was replaced by that of connecting the cover into Mosin. The excavated clothes show three kinds of textile fabrics: plain silk fabrics and plaited silk, plain twill and four-leaf-patterned twill, eight-leaf-plain satin of life-lettered textile and five-leaf satin damask, and plain satin. Especially, the combination of eight-leaf satin and four-leaf twill with mixed textile is considered as a fabric of high quality. The excavated clothes of Lady Sim Cheongsong showed a periodical change which was different from the clothes of the 18th century in terms of the formal composition of Jegori and Yeomo. Regarding Women's Jegori a short length and slim and long sleeves are changed into short and tight Jegori, which signaled the specific change of Jegori aesthetics. The significance of the excavated clothes of Lady Sim Cheongsong lies in its role as the bases for understanding the couture culture of the 19th century.

Far-infrared Emission Characteristics of ZrC Imbedded Heat Storage Knitted Fabrics for Emotional Garment (탄화지르코늄 함유 감성의류용 축열/발열 편물의 원적외선 방출특성)

  • Kim, Hyun-Ah
    • Science of Emotion and Sensibility
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    • v.18 no.1
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    • pp.49-58
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    • 2015
  • This paper investigated far-infrared emission characteristics of ZrC imbedded heat storage knitted fabrics for emotional garment. For this purpose, ZrC imbedded heat storage PET was spun with high viscosity PET imbedded ZrC powder on the core part and low viscosity PET on the sheath part by conjugated spinning method. Ingredient analysis and far-infrared emission characteristics assessment of spun filament were carried out by EDS and FT-IR spectrometer. Two kinds of knitted fabrics were made using texturized ZrC imbedded PET for measuring thermal characteristics of ZrC imbedded heat storage PET. Zr peak was certified by EDS measurement and it was confirmed that content of Zr was 19.29%. Far-infrared analysis revealed that emission power at the range of wavelength, $5{\sim}20{\mu}m$ was $3.65{\times}10^2W/m^2$, and emissivity was 0.906. Heat storage analysis by KES-F7 system revealed that $Q_{max}$ of ZrC imbedded PET knitted fabric was lower than that of regular PET one and warmth keepability rate was higher than that of regular one, which means that ZrC imbedded PET knitted fabric has heat storage property. Thermal conductivity of ZrC imbedded PET knitted fabric was higher than that of regular PET one which was caused by high thermal conductivity of Zr itself. Hand property of ZrC imbedded knitted fabric was not inferior compared to regular PET knitted fabric, which preferably was found to be dependent on knit structure and surface property.