• Title, Summary, Keyword: fipronil

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Efficacy of Maxforce and Avion gel baits containing fipronil, clothianidin, and indoxacarb against the German cockroach (Blattella germanica)

  • DAVARI, Behroz;KASHANI, Susan;NASIRIAN, Hassan;NAZARI, Mansour;SALEHZADEH, Aref
    • Entomological research
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    • v.48 no.6
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    • pp.459-465
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    • 2018
  • The present study evaluated the efficacy of Maxforce gel baits containing 0.01% fipronil (Maxforce), 0.05% fipronil (Maxforce Magnum), 1.0% clothianidin (Maxforce Impact), and 0.6% indoxacarb (Avion gel bait; all from Bayer Environmental Science, Clayton, CA, USA) against reference and wild strains (Mobasher, Andisheh, Tamin-e Ejtemaei, and Maskoni) of German cockroach collected in 2016. Test design and bait placement were according to World Health Organization (WHO) procedures for efficacy achieving a 95% mortality rate. Although cockroaches fed on all gel baits, gel baits containing clothianidin and indoxacarb were eaten at higher rates than gel baits containing 0.01% and 0.05% fipronil. The cumulative mortality rates reached 100% for all cockroach strains at 32, 28, 28 and 40 h after eating baits containing 0.01% fipronil, 0.05% fipronil, clothianidin, and indoxacarb, respectively. Efficacy (a 95% mortality rate) after exposure (consumption) of gel baits containing 0.05% fipronil and clothianidin was seen after 28 h, compared with 32 h for 0.01% fipronil and 40 h for indoxacarb. In conclusion, commercial gel baits have improved and may be more effective than spray formulations against German cockroaches.

Development of Analytical Method for Fipronil Residues in Agricultural Commodities Using GC-ECD/MS (GC-ECD/MS를 이용한 농산물 중 Fipronil의 잔류 분석법 개발)

  • Ahn, Kyung-Geun;Kim, Gyeong-Ha;Kim, Gi-Ppeum;Hwang, Young-Sun;Kang, In-Kyu;Lee, Young Deuk;Choung, Myoung-Gun
    • Korean Journal of Environmental Agriculture
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    • v.34 no.4
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    • pp.309-317
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    • 2015
  • BACKGROUND: An analytical method was developed using GC-ECD/MS to precisely determine the residue of fipronil, a phenylpyrazole insecticide used to control a wide range of foliar and soil-borne pests.METHOD AND RESULTS: Fipronil residue was extracted with acetone from representative samples of five raw products which comprised hulled rice, soybean, Kimchi cabbage, green pepper, and apple. The extract was diluted with saline water, and fipronil was partitioned into n-hexane/dichloromethane (20/80, v/v) to remove polar co-extractives in the aqueous phase. Florisil column chromatography was additionally employed for final purification of the extract. Fipronil was separated and quantitated by GC-ECD using a DB-17 capillary column. Accuracy of the proposed method was validated by the recovery from crop samples fortified with fipronil at 3 levels per crop in each triplication.CONCLUSION: Mean recoveries ranged from 86.6% to 106.0% in five representative agricultural commodities. The coefficients of variation were less than 10%. Limit of quantitation of fipronil was 0.004 mg/kg as verified by the recovery experiment. A confirmatory technique using GC/MS with selected-ion monitoring was also provided to clearly identify the suspected residue. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of fipronil in agricultural commodities.

Development of Analytical Method for Fipronil and Fipronil-Sulfone in Animal Serum by LC-MS/MS (LC-MS/MS에 의한 동물 혈청 내 피프로닐 및 피프로닐 설폰 분석법 개발)

  • Lee, Jeongsun;Park, Na-Youn;Jung, Woong;Kho, Younglim
    • Journal of the Korean Chemical Society
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    • v.63 no.6
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    • pp.415-419
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    • 2019
  • Fipronil is an insecticide that belongs to the N-phenylpytazole and has been used mainly for an insect pest control. However, it is known that acute poisoning of the human body causes various symptoms such as dizziness, muscle weakness, dyspnea, skin irritation, and increased heart rate. Lately, eggs containing fipronil have been distributed and toxic problems are spreading around the world. In this study, we tried to develop analytical methods to evaluate the exposure of fipronil and fipronil sulfone in animal serum samples. The differences according to mobile phase and the results of liquid - liquid extraction and solid phase extraction pretreatment method were compared. Distilled water (A) and acetonitrile (B) were selected for the mobile phase, and the pretreatment method was determined by solid phase extraction. As a result of the method validation, the intra-day / inter-day accuracies were 82.2~114.1% and the precisions were less than 20%. The detection limit was 0.027 ng/ml for fipronil and 0.087 ng/ml for fipronil sulfone. The linearity obtained was satisfying, with a coefficient of determination (r2) higher than 0.99. The concentrations in some animal sera were determined using the methods of analysis for fipronil and fipronil sulfone in animal sera developed in this study. Using the method developed in this study, it could be used as an analytical method for human bio-monitoring of fipronil and fipronil sulfone as well as animal serum.

Susceptibilities of German Cockroach, Blattella germanica to Insecticides According to Application Methods (바퀴에 대한 처리방법에 따른 살충제 감수성)

  • Han Jongbeen;Kim Gil-Hah
    • Korean journal of applied entomology
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    • v.43 no.3
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    • pp.241-247
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    • 2004
  • Susceptibility of Blattella germanica male adults to 61 commercial insecticides was evaluated by diet dipping method. Among them three insecticides of dichlorvos, fenitrothion, and fipronil showed over $90\%$ mortality. The insect was more susceptible to dichlorvos and fenitrothion in filter paper contact method than in diet dipping one, but fipronil was vice versa. Toxicities of the three chemicals were evaluated by application parts of the such as head, thorax, abdomen, and legs. Dichlorvos and fenitrothion were more toxic when applied to head, and flpronil was thorax. $LT_{50}$ values by diet dipping method showed that fenitrothion and fipronil acted more rapidly than boric acid and hydramethylnon. The values of the former two were 1.05 and 0.98 days, and those of the latter two were 3.92 and 2.26 days, respectively.

Risk Assessment of Fipronil on Honeybee (Apis mellifera) (Fipronil의 꿀벌 (Apis mellifera)에 대한 위해성 평가)

  • Kim, Byung-Seok;Yang, Yu-Jung;Park, Yeon-Ki;Jeong, Mi-Hye;You, Are-Sun;Park, Kyung-Hun;Ahn, Young-Joon
    • The Korean Journal of Pesticide Science
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    • v.13 no.1
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    • pp.39-44
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    • 2009
  • This study was conducted to evaluate the actual risk of fipronil on worker honey bees (Apis mellifera L.) through acute contact toxicity test, acute oral toxicity test, toxicity of residues on foliage test, and small scale field test. The $48h-LD_{50s}$ of fipronil SC on honeybee were $0.005{\mu}g$ a.i./bee in acute contact toxicity test and $0.004{\mu}g$ a.i./bee in acute oral toxicity test, respectively. In toxicity of residues on foliage test, fipronil showed over 90% of mortality during 28days after treatment at recommended application rate. The $DT_{50}$ of dislodgeable foliar residue was 9 days. Finally, In small scale field test, fipronil showed similar toxicity in the residues on foliage test. It was concluded that fipronil has very high acute toxicity and long residual toxicity to honeybee. Therefore, fipronil is highly toxic to bees exposed to direct treatment or residues on blooming crops or weeds. Do not apply this product or allow it to drift to blooming crops or weeds if bees are visiting the treatment area. To protect honeybee and wild pollinators from outdoor use of fipronil, ultimately it should need to limit for only indoor use to prevent pollinators from unintentionally exposure of fipronil.

Efficacy of Fipronil-applied Canine Hair against House Dust Mites (Fipronil 적용 개 피모의 집먼지 진드기의 살진드기에 대한 효과)

  • 강선미;이충호;박옥지;임지혜;윤희정;권오경
    • Journal of Veterinary Clinics
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    • v.19 no.2
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    • pp.215-218
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    • 2002
  • It is known that house dust mites which settle on sofa, carpet, and dust in the house ignite asthma and allergic rhinitis. Dermatophagoides farinae and Dermatophagoides pteronyssinus distribute widely and densely in Korea. In this study we carried out to identify that shed hair of dogs which was applied topically with fipronil ($\Frontline^{circledR}$) kill house dust mites. We co-cultured house dust mites with fipronil-applied hair of dog during 12 hours, and then compared the death rate of mites here with that cultured without hair. The larger amount of hair, the more mites were killed. The death rate of mites was higher than control significantly until 2 weeks after $\Frontline^{circledR}$ application. Hair which had been shed at 3 days after $\Frontline^{circledR}$ application always killed the smaller number of mites than not shed. The present study suggested that the use of $\Frontline^{circledR}$ to our pets control house dust mites effectively.

Ingestion Toxicity of Fipronil on Reticulitermes speratus kyushuensis (Isoptera: Rhinotermitidae) and Its Applicability as A Termite Bait

  • Kim, Si Hyun;Chung, Yong Jae
    • Journal of the Korean Wood Science and Technology
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    • v.45 no.2
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    • pp.159-167
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    • 2017
  • Historical wooden buildings in Korea are being damaged by Reticulitermes speratus kyushuensis Morimoto, a type of subterranean termite, and the scale of this damage is increasing gradually because of global warming. This study evaluated the ingestion toxicity of the phenylpyrazole insecticide fipronil to R. s. kyushuensis and its applicability as termite bait with the aim of controlling termite colonies more efficiently. An ingestion toxicity assessment was conducted and the $LT_{50}$ was determined to be 4.43 day at concentrations of 10 ppm, indicating a slow-acting effect; therefore, 10 ppm was selected as an appropriate dose. A field applicability assessment was conducted in which the number of foraging workers and the rate at which the termites fed decreased 2 weeks after baiting, and termite colony activity was no longer apparent after 4 weeks demonstrating the efficacy of fipronil at eliminating colonies. Taken together, these results, indicate that low-dose fipronil eliminated R. s. kyushuensis colonies faster than insect growth regulators; therefore, it is expected to be useful when trying to conserve historical wooden buildings.

Sol-gel TiO2/Carbon Paste Electrode Nanocomposites for Electrochemical-assisted Sensing of Fipronil Pesticide

  • Maulidiyah, Maulidiyah;Azis, Thamrin;Lindayani, Lindayani;Wibowo, Dwiprayogo;Salim, La Ode Agus;Aladin, Andi;Nurdin, Muhammad
    • Journal of Electrochemical Science and Technology
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    • v.10 no.4
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    • pp.394-401
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    • 2019
  • The unique study of TiO2 sol-gel modified carbon paste electrode (CPE) nanocomposites have been developed for electrochemical sensor detecting fipronil pesticide compound. We develop the easy synthesized TiO2 via a sol-gel method and modified in CPE which applied electrochemical system as cyclic voltammetry (CV) because the concentration is proportional with current peaks. We discover the TiO2 optimal mass used of 0.1 g which is compared with 0.7 g carbon and 0.3 mL paraffin. It has high-current anodic (Ipa) of 1.13×103 μA and high-current cathodic (Ipc) -0.96×103 μA in scan rate of 0.5 V/s. The limit of detection (LOD) of fipronil has been determined of 34.0×10-5 μM in percent recovery of 0.8%. Its high-stability for lifetime TiO2-CPE nanocomposites was expressed for 13 days which mean that can be used for detecting fipronil pesticide.

Field trial on the control effect of fipronil bait against German cockroaches

  • Ree Han-Il;Lee In-Yong;Jeon Soung-Hoo;Yong Tai-Soon
    • The Korean Journal of Parasitology
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    • v.44 no.3
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    • pp.255-257
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    • 2006
  • A field trial on the control effect of fipronil poison bait against German cockroaches (Blatella germanica) was carried out at different restaurant types in Sinchon, Seoul, Republic of Korea. Monitoring was performed applying food baited traps for 2 days per week. Reduction rates of German cockroaches by applying fipronil baits were 90.9% at Korean restaurants, 96.4% at Chinese restaurants, and 89.4% in beer hall kitchens after 4 weeks of the treatment. Overall average of the reduction rate was 93.9%. As the natural reduction rate at untreated restaurants was 11.5% after 4 weeks, a correction of the average reduction rate by applying the Abbot formula was 93.1 %.